En-Min You, 尤恩民

博士 / 國立臺灣大學 / 動物學研究所 / 96 / The black tiger shrimp (Penaeus monodon) is an ecologically and economically important marine crustacean and is widely distributed in the Indo-Pacific region. Here I investigated the genome of P. monodon in two aspects, i.e., (1) the genetic diversity of wild populations, (2) the construction of a genetic linkage map. There are 4 chapters in this thesis. Chapter 1 contained the paper review of the research topics, the research methods, and the research aspects. In ...

Shr-Lung Jau, 趙士龍

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / The Sergia 1ucens is the main species of the sergestid shrimp fisheries in Taiwan. There had very little basic studies about the Sergia 1ucens around the northeastern waters of Taiwan since most of the documented data were upon that in the southwestern waters of Taiwan. Current investigation focused on the seasonal variation in its population dynamics with data biological and ecological parameters in surrounding waters of Gui-San Island, northeastern Taiwan. The inv...

Yu-Wen Yang, 楊宇雯

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / This study investigated the effects of bacterial cells and extracellular products (ECP) of Vibrio alginolyticus on clotting factors and hemocyanin of white shrimp Litopenaeus vannamei. The oxyhemocyanin in hemolymph of white shrimp differed at different molt stage (A、B、C、D stage) with lowest concentration at A stage and highest at D stage. The changes of genetic expression (mRNA) of the coagulogen, transglutaminase and hemocyanin in hepatopancreas of white shrimp di...

Guan-James Wu, 吳冠政

博士 / 國立臺灣海洋大學 / 食品科學系 / 94 / The aims of this research are to investigate the hydrolytic activity of cellulase on chitosan, and to evduate in vitro and in vivo the immunomodulation activity of the hydrolytic products. The optimal reaction pH and temperature for cellulase digestion of chitosan with 95% deacetylation degree (DD95) were pH 5.2 and 55oC, respectively. Its hydrolystic activity increased with the increasing DD of chitosan. Using fed-batch technique, the productivity of the chitosan hy...

Chun-Wei Chen, 陳純葳

碩士 / 國立中興大學 / 植物病理學系所 / 94 / Tomato Fusarium wilt is an important disease during summer season in Taiwan. Tomato seedlings were planted in nature soil and infested soil respectively treated with ten different organic amendments for seven days. Amendments of soil with rice husk, shrimp and crab shell powder, and oyster shell powder were able to enhance tomato growth and reduced disease severity of tomato Fusarium wilt. Application of shrimp and crab shell powder was significantly effective in ...

Yeong-Chang Luo, 羅永昌

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 92 / The white shrimp, Litopenaeus vannamei, is distributed along the eastern Pacific coast from northern Mexico to northern Peru. This species inhabits the lagoon and river mouth with a wide range of salinities and dissolved oxygen. For further comprehension about shrimp at various salinities, we tried to evaluate the white shrimp’s growth, including its survival rate, growth rate, feed conversion rate and condition factor of juveniles at 4 different salinities (0,...

Shi-Hong Wang, 王世弘

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / Abstract This study examined the immune response of the white shrimp Litopenaeus vannamei, and its resistance against Vibrio alginolyticus when the shrimp were injected with chitin and chitosan. L. vannamei injected with chitin at dose of 4 μg g-1 or 6 μg g-1 were examined for the immune parameters after 1, 2, 4 and 6 days. L. vannamei injected with chitin at a dose of 6 μl g-1 increased significantly its total haemocyte count (THC) after 2 days. L. vannam...

Long-Uong Wang, 王融雍

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / Abstract The objective of this research is aimed to document the immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus under the changes in salinity and temperature. White shrimp L. vannamei held in 25 ‰ were injected with TSB-grown V. alginolyticus（1 x 104 cfu/shrimp), and then transferred to 5, 15, 25 and 35 ‰. Over 48-96 h, the mortality of V. alginolyticus-injected shrimp held in 5 ‰ and 15 ‰ was significantly highe...

Shan-Ju Tai, 戴珊如

碩士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Abstract This study was conducted to elucidate the effects of lactoferrin (LF) on growth and immune responses in juvenile grass shrimp, Penaeus monodon. Basal diet supplemented with seven levels (0, 100, 300, 700, 1,000, 2,000, 5,000 mg/kg diet) of LF were each fed to triplicate groups of shrimp (initial weight 0.15 ± 0.01 g) for 8 weeks. Shrimp fed diets supplemented with ≧ 2,000 mg LF/kg had significantly (P＜ 0.05) higher weight gain than shrimp fed diets with ≦ 70...

Chin-Ying Wang, 王姿穎

碩士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Abstract To investigate the utilization of β-carotene and its bioconversion to vitamin A in juvenile grass shrimp (Penaeus monodon), two growth trials were conducted. In experiment I, semi-purified diet contained seven levels of β-carotene (βC) (0, 30,000, 60,000, 90,000, 120,000, 150,000, 200,000 IU/kg diet) together with a reference diet containing 8,400 IU/kg diet of vitamin A (retinyl acetate, VA) were each fed to triplicate groups of shrimp (mean initial weight...

Hao-ling Kang, 康浩琳

碩士 / 國立中山大學 / 海洋資源學系研究所 / 94 / Zero-exchange system doesn’t discharge the nutrient-rich pool water to the environment, so it doesn’t cause the pollution. Besides, nutrients in the water can be re-used and the amount of water used can be reduced. In shrimp pond, ammonia and nitrite will accumulate during the period of cultivation because of feeding large amount of protein-rich feed. In traditional culture system, a great deal of water was used to solve the problem. In zero-exchange system, nitro-...

Tzu-Hsuan Yang, 楊子萱

碩士 / 國立臺灣大學 / 微生物與生化學研究所 / 92 / Antimicrobial peptides (AMPs) were well known to play an important role in the innate host defense system of living organisms. Generally, these defensive peptides are small cationic molecules and act against invading microorganisms such as bacteria, fungi, parasites and virus. Moreover, microorganisms hardly evolve the resistance toward antimicrobial peptides but the conventional antibiotics. Recently, some studies showed that the AMPs could be successfully applie...

Min-DeYeh, 葉民德

碩士 / 國立成功大學 / 生物科技研究所 / 104 / That acute hepatopancreatic necrosis disease (AHPND) can cause up to 100% mortality in post-larvae shrimp makes it a costly problem for the shrimp industry. The etiology of AHPND is Vibrio parahaemolyticus (VP) which has a unique plasmid containing toxic genes, PirA and PirB. Our laboratory has already reported the basic pathogenesis of AHPND. However, the immune response of AHPND-infected shrimp remains unknown. In this study, we focus on two immune pathways, Toll ...

Huang, Chang Yi, 黃長義

碩士 / 國立海洋大學 / 水產養殖研究所 / 84 /

Xu, Jia Zhen, 許嘉珍

碩士 / 國立海洋大學 / 水產食品科學研究所 / 84 /

Huang, Chang-Yi, 黃長義

碩士 / 國立海洋大學 / 水產養殖學系 / 84 / Outbreaks of a disease causing serious losses among population of culturedPenaeus japonicus in Taiwan are characterized by obvious white spots on the carapace, appendages and the iside surface of the body.The diseased shrimp alsodisplay signs of lethargy and reddish coloration of the hepatopancreas.For thisreason,Chou et al.(1995) and Wang et al.(1995)propose the name "white sp...

Chang, Pi-Erh, 張碧兒

碩士 / 國立海洋大學 / 水產養殖學系 / 84 /

Chung-Ming Chen, 陳仲銘

碩士 / 國立臺北教育大學 / 文化創意產業經營學系 / 103 / The paper presents how decoration and marketing strategy attract customers. Another aim is to find out whether the elements are important to a themed restaurant. The study focused on a prawn-themed restaurant, Bai Jia Ban Prawn Restaurant Shanghai branch. After combining the images of bamboo and prawn, new design of restaurant and menu used the combined image. Through the questionnaire survey and analysis, the combined design can impose the restaurant’s impre...

Lin, Ruei-Shiuan, 林睿軒

碩士 / 國立臺灣大學 / 動物學系 / 85 / Protein geranylgeranyltransferase I was purified from the eyes of shrimp Penaeus japonicus by the sequential column chromatography on HiTrap Q, Superdex 200, Mono Q and peptide- coupled affinity column. The purified enzyme was found to have relative Mr of 66 kDa as estimated by SDS-PAGE. Since the active protein geranylgeranyltransferase I from Superdex 200 was found to have relati...

Lu, Chia-Wen, 盧佳彣

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 103 / Progranulin (PGRN), a multi-functional gene, can regulate cell proliferation, survival, wound healing, embryonic development, and inflammation. PGRN consists of multiple Granulin (GRN) units. In this study, we identified PGRN gene in white shrimp (Litopenaeus vannamei), designated Lvpgrn, which is frist discovered in Crustacea. We used RACE-PCR to obtain the full-length cDNA of Lvpgrn from white shrimp haemocytes. The full-length cDNA of Lvpgrn was 2640 bp in lengt...

Pei Lin Ho, 何珮琳

碩士 / 國立屏東科技大學 / 水產養殖系所 / 98 / The purpose of this study was to isolate and identify a beneyicial bacterium, Bacillus subtilis E20, from natto (fermented soybeans), and incorporate it into shrimp (L. vannamei) feed to promote shrimp (L. vannamei) growth performance. A protease-producing bacterium, E20, isolated from natto was identified as B. subtilis by an API 50 CHB kit and the 16S rDNA sequence. B. subtilis E20 was able to grow at a broad range of temperatures (10–50℃), pH values (5–10), and N...

chiu hung tien, 邱泓典

碩士 / 國立屏東科技大學 / 水產養殖系 / 92 / Physiological and immunological factors in the hemolymph of white shrimps, Litopenaeus vannamei, were analyzed at before treatment and at 2, 4, 8, 16 and 24 hrs after injections of 0, 10-8, 10-7 and 10-6 mole dopamine. phenoloxidase activities, superoxide anion contents, total haemocyte count, Superoxide dismutase activity, phagocytosis activities and clearance efficiencies were decreased significantly. Hemolymph glucose, lactate, sodium ion, chloride ion, osmolariti...

Ching-Yi Lai, 賴靜怡

碩士 / 國立屏東科技大學 / 水產養殖系 / 92 / Prophenoloxidase activation system which plays an important role in non-self recognition and immune response, is considered as a major defense mechanism in invertebrates especially in insect and crustacean. A close relationship between the proPO activation system expression and the immunity in shrimps has been reported in the past. We have cloned prophenoloxidase gene from the white shrimp (Litopenaeus vannamei) using reverse transcription polymerase chain reaction (...

Po-Chih Chuang, 莊博智

碩士 / 國立屏東科技大學 / 獸醫學系 / 93 / Viruses are the most major pathogens of shrimp, and they can induce high morbidity, mortality and growth retardation in cultured shrimp. Among them, white spot syndrome virus ( WSSV ), infectious hypodermal and haematopoietic necrosis virus ( IHHNV ), Taura syndrome virus ( TSV ) and yellowhead virus ( YHV ) often cause heavy economic losses. The present study is to investigate the viral diseases of exported broodstock shrimp by means of pathologic and molecular metho...

Chiang, Pei-Chi, 姜佩琪

碩士 / 國立屏東科技大學 / 水產養殖系 / 95 / Blood coagulation is an essential immune mechanism for aquatic animals with an open circulatory system such as crustaceans. This research is aimed to comprehend the molecular characteristics of the clottable protein in white shrimp, Litopenaeus vannamei, through molecular cloning of its cDNA and tissues expression, profiles. The expression of CP transcripts under different temperature, salinity and ammonia and post-Vibrio alginolyticusc injection were also investgate...

Chia-Wen Hung, 洪嘉文

碩士 / 國立屏東科技大學 / 水產養殖系 / 94 / A coagulation system is essential in both invertebrate and vertebrate animals to prevent excess blood loss from wounds and obstruction of invading microorganisms via the wounds. Transglutaminase (TG) (EC 2.3.2.13) is the only molecule conserved in the coagulation systems of all known invertebrate species. TG cDNA is cloned from the haemocytes of white shrimp (Litopenaeus vannamei) by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers. T...

Jian-Cheng Zhu, 朱簡承

碩士 / 國立宜蘭大學 / 生物資源學院碩士在職專班 / 103 / Sergestid shrimp (Sergia lucens Hansen, 1922) being one of the plankton can be directly used by humans. The main producing areas are the Japanese Suruga Bay and the southwest waters of Tungkang, Pingtung, and the northeastern waters of Gueishan Island, Yilan, Taiwan. The past studies mainly focused on ecological characteristics, reproduction, processing and its related applications. This study will focus on the fisheries production and morphological comparison ...

Huang, Ken-Meng, 黃耿盟

博士 / 國立臺灣海洋大學 / 海洋生物研究所 / 103 / Attempts were first made to identify the possible causative agents and species in a fishing shrimp poisoning case occurred in Keelung, North Taiwan, 2006. The patient’s symptoms were more similar to those of illegal food additive boric acid poisoning or those of legal food additive sulfite poisoning. To establish the species identification of poisoned shrimp sample, 10 kinds of commercial shrimp species were collected, identified species by judging for outside app...

Chang, Chun-Po, 張鈞博

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / The purpose of this reearch is to explore the effect of various feeds on ovary development and maturation of Yamato shrimp (Caridina mutidentata) further to establish breeding techniques and feeding scheme. Moreover, estrogen and androgen was examined to investigate the growth and sex control of juvenile shrimp. Observation of Yamato shrimp ovarian stage 1 development through 66 weeks, it was presented with a significant increase from the 2nd to the 12th week and, ...

Wu, Shu-Han, 吳書瀚

碩士 / 國立臺灣海洋大學 / 食品科學系 / 104

Liang, Zheng-Rong, 梁正融

碩士 / 國立臺灣海洋大學 / 食品科學系 / 104 / Due to polyphenol oxidase enzyme melanosis, microorganism and lipid oxidation, shelf life of shrimp under normal storage conditions is very short. In general, sulfite was used in shrimp to prevent from enzymatic browning and inhibiting microbial growth. However, over used by food processors is the major concern. In addition to this, consumers now concern the impact of chemical additives on the human body. There is a growing trend using natural substances or physical...

Chu,Pei-Yun, 朱珮云

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104

Ying-Yu Liu, 劉盈妤

碩士 / 國立臺灣大學 / 漁業科學研究所 / 104 / This study use phototaxis of white shrimp which attracts shrimp without moltingand reduces them to attack shrimp in molting, so that we can design aculture environment following the habitats of white shrimp to reduce cannibalism of shrimp, and indirectly improve shrimp survival. In this study , we use four color light which blue (442nm), green (525nm), yellow (590nm) and red (639nm) light, three illuminance which 100lux、500lux、1000lux, and four stocking density whic...

Chia-Ling Lee, 李佳齡

碩士 / 國立屏東科技大學 / 水產養殖系所 / 99 / The present main threat for global shrimp industry is still the hazard of viral disease. The immunity of shrimp is just with a non-specific innate immune system. It cannot protect the shrimp against the attack by most of the viruses. It always makes a serious loss of farmers whenever the virus invaded into the shrimp farm. Taiwan’s shrimp aquaculture has been fallen down dramatically by the attack of viruses since 1987, not only the production decline severely, but...

Kuo, Chia-Ling, 郭佳陵

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 105 / Panax notoginseng (family: Araliaceae) is a common traditional Chinese medicine that has been shown to improve immune, antioxidant, and anticancer functioning. To investigate the regulatory roles of Panax notoginseng water extracts (PNWE) in the physiological and digestive functions and immune response of white shrimp (Litopenaeus vannamei), they were individually injected with the PNWE at 50, 100, and 200 μg (g shrimp)-1 for 0, 12, 24, 48, 72, 168 and 240 hou...

Su, Che-Wei, 蘇則瑋

碩士 / 國立屏東科技大學 / 食品科學系所 / 104 / The Contents of Abstract in This Thesis: In this study, twenty lactic acid bacteria strains were respectively added into defat soybean meals for fermentation and general ingredients of the fermented defat soybean meals, and the proximate composition were analyzed for screening optimal strains. Antibacterial test and characteristic analysis including growth curve, titratable acidity, and pH value of the selected lactic acid bacteria strains were conducted. Prelimina...

Da-Ji Huang, 黃大駿

博士 / 國立臺灣大學 / 動物學研究研究所 / 93 / The purpose of this study was to investigate the chronic and reproductive toxicity of chlordane and lindane as well as their endocrine disruption effect on Green Neon Shrimp (Neocaridina denticulata), a common habitant in freshwater system of eastern Asia and Hawaii. In this study, the very low experimental concentrations 1 and 10 ng/L of chlordane and 0.1 and 1 μg/L of lindane were used, and biochemical composition change in hemolymph, growth rate, duration of molt...

Yu -Chin Yu, 游玉琴

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 93 / The objective of this study is aimed to understand the toxicity of copper sulfate and its effects on the physiological and immune responses to white shrimp(Litopenaeus vannamei ). The acute toxicity of copper sulfate was experimented, and 50 % lethal concentration(LC50)was further calculated.。Physiological parameters of hemolymph lactate, glucose and triglyceride, and the immune parameters of the activities of phenoloxidase, reactive oxygen intermediate and su...

Yu-Chen Wu, 吳育甄

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / Abstract The present thesis investigated the effects of pathogenic Vibrio harveyi on blood coagulating mechanism and hemocyanin of white shrimp Litopenaeus vannamei. The unclottable hemolymph of shrimps affected by the injection of bacterial cells or extracellular products ( ECP ) of V. harveyi could be re-clottable by the addition of 0.2 U/ml transglutaminase and 4mM CaCl2 ( clotting factors ). This phenomenon could be found by visualizing the occurrence of larger...

Yu-Chun Cheng, 鄭宇君

碩士 / 國立臺灣海洋大學 / 食品科學系 / 93 / Two experiments were conducted to quantify the optimum dietary p-aminobenzoic acid (PABA) requirements of juvenile grass shrimp (Penaeus monodon) and juvenile grouper (Epinephelus malabaricus). Experiment I. Semi-purified basal diet supplemented with p-aminobenzoic acid at 0, 100, 300, 600, 1000, 1500, 3000 and 5000 PABA mg/kg diet providing the actual dietary value of 0, 108, 309, 603, 1027, 1501, 3060, 5233 mg PABA/kg diet, respectively. Each diet was fed to tripli...

Zeng Tai Huang, 黃正臺

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / Abstract There were three immunostimulants, β-glucan, Laminarin and Sodium alginate, have been added to diets with 0g/kg, 1g/kg and 4g/kg respectively and fed shrimps in this study. At day 0、1、2、4、8、16 and 30 after feeding, shrimps were sampled in order to examine the variance of Total haemocyte count (THC)、Phenoloxidase (PO)、Superoxide anion (O2-)、Superoxide dismutase (SOD) 、Total protein (TP)、Transglutaminase (GOT、GPT)、Cholesterol (CHOL)、Triglycerides (TG) and Gl...

Min-Shuo Hsieh, 謝旻朔

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99

Pei-Hsin Huang, 黃姵馨

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / Probiotics, which are micro-organisms and their products with health benefit to the host, have used in aquaculture as a means of disease control, supplementing or even in some cases replacing the use of antimicrobial compounds. Water quality is the most important essentials in aquacluture industry. In past, it has been shown most probiotics can for improving water quality, are phototrophic bacteria and nitrifying bacteria. The objective of presen...

Tzu-Chi Kung, 龔子棋

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / This study uses in vitro and in vivo experiment to investigate the effect of medicinal plant complex (Xiao-Chai-Hu decoction) on non-specific immune response of white shrimp (Litopeneaus vannamei). To determine prophenoloxidase activity (proPO), reactive oxygen species production (ROS), superoxide anion production (O2-), nitric oxide production (NO), phagocytic rate (PR), phagocytic index (PI), superoxide dismutase activity (SOD), total haemocyte count (THC), differ...

Sian-Yin Jhou, 周先音

碩士 / 國立高雄海洋科技大學 / 漁業生產與管理研究所 / 98 / The bottom trawl is one of the important inshore fisheries in Pingtung, Taiwan. However, it frequently damages the breeding habitats and makes a great impact on the fish resources so that the government implemented the closed fishing areas within 3 nms from the coastline for trawlers. In order to understand the differences of catche components between inside and outside of closed fishing areas, this study used the shrimp beam trawl to carry out the experiments...

Ching-Lin Su, 蘇景琳

碩士 / 國立中山大學 / 機械與機電工程學系研究所 / 99 / Fairy shrimp is an anostraca plankton which is raised for watching and scientific study. In recent year, due to the large requirement of fairy shrimp in the market, to increase the hatching rate of fairy shrimp is an important topic. It is found that when an Artemia Cysts hatched by light, the pigment absorption spectra in the Cysts match with the optical wavelength of the experimental incubators. Furthermore, the effect of ultrasound can stimulate the growth or...

Tsai, Wan-Ru, 蔡宛汝

碩士 / 國立高雄餐旅大學 / 台灣飲食文化產業研究所 / 103 / “Three treasures of Donggang – bluefin tuna, sakura shrimp, and oilfish roe.” Donggang is famous for its fishing industry, which is why seafood dominates the majority of its local cuisines. In recent years, Donggang made its local tourism fame by its slogan the “three treasures of Donggang.” As culinary tourism continued to flourish, the local special cuisine tourism also became one of sightseers’ favorites, and caused the rise of the Bluefin Tuna Cultural Fes...

Haiao-wei-jen, 蕭惟仁

碩士 / 大葉大學 / 食品工程研究所 / 89 / This thesis is a study of the utilization of shrimp shell wastes by Monascus spp. to produce antifungal substance. The purification and characterization of fungicides were described. By resulting, we choose Monascus 31499 as the fungicide producer. That inhibitory activity（0.35U/ml）for Fusarium oxysporum was obtained when the strain was grown aerobically in a medium consisting of 1% shrimp shell wastes，0.l% K2HPO4 、0.05% MgS04 ．7H20 、0.001﹪FeSO4．7H2O 、0.3﹪Na...

Yu-Xain Tzeng, 曾裕憲

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 96 / Part 1. Tilapia (O. niloticus (♀) × O. aureus (♂)) is one of the most important aquaculture fish in Taiwan. Taiwan has gained worldwide reputation in frozen tilapia fillet processing technology. This study was aimed to investigate the relation of qualities between raw fish and the frozen fillet, in order to found the certification index. At first, tilapia was scarified and the round fish was stored in ice or at 25°C for due time intervals, and then provided for...

林珍君

碩士 / 大葉大學 / 食品工程學系碩士班 / 91 / The production of useful protease by Monascus purpureus CCRC31499, using shrimp and crab shell powder as the major carbon source, was studied. The optimized conditions for protease production was found when the culture was shaken at 37℃ for 4 days in 100mL of medium（pH7）containing 2﹪shrimp and crab shell powder, 0.15﹪poly-peptone, 0.1﹪yeast extract, 0.1﹪K2HPO4 and 0.05﹪MgSO4 •7H2O. The protease of Monascus purpureus CCRC31499, produced under the optimized ...