WenRen Gua, 高文仁

碩士 / 國立屏東科技大學 / 水產養殖系所 / 98 / 不公開

Wen-Hui Lin, 林文惠

碩士 / 國立屏東科技大學 / 獸醫學系所 / 95 / Taura syndrome ( TS ) and white spot syndrome ( WSS ) spreaded throughout the world, and have been detected in cultured and captured shrimp, crabs and other arthopods. In this study, a total of 220 pacific white shrimp ( Litopenaeus vannamei ) cultured farms in Taiwan and 20 shrimps were random sampled from each farm to detect taura syndrome virus ( TSV ) by reverse transcription polymerase chain reaction ( RT-PCR ) and white spot syndrome virus ( WSSV ) by polymeras...

Chiung-Hui Tsai, 蔡瓊慧

碩士 / 國立屏東科技大學 / 水產養殖系 / 93 / LGBP cDNA was cloned from the haemocytes and hepatopancreas of white shrimp Litopenaeus vannamei by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers. Analysis of nucleotide sequence revealed that the cDNA clone has an open reading frame of 1101 bp encoding a protein of 367 amino acids including a 17 amino acid signal peptide. The calculated molecular mass of the mature proteins is 39.92 kDa with an estimated pI of 4.37. Two putative i...

Wei-Shiou Chuang, 莊偉秀

碩士 / 國立中興大學 / 植物病理學系所 / 101 / Fusarial wilt of watermlon is caused by soil-borne fungal Fusarium oxysporum (Schl.) f. sp. niveum (E. F. Smith) Snyder & Hansen, and it is hard to be controlled. Chemical fungicides and grafting bottle gourd (Lagenaria siceraria Ser.) are mainly used to control the disease in Taiwan. In order to search alternative methods, the purpose of this study is to use amendments and microorganism to develop a bioformulated cultural medium for controlling watermelon fusarial ...

SHU-LING, HSIAO, 蕭淑玲

碩士 / 輔仁大學 / 餐旅管理學系碩士在職專班 / 105 / In recent years, result from the increasing penetration and utilization of the internet, the consumption behavior of countryman on the internet has been increased also. Therefore, the study is aimed at the influence of the consumers between advertising appeals and advertising effects on the users to execute the online food purchase behavior. In addition to explore two factors, the type of spokesperson and physical risk, to measure the physical risk cognition deg...

Huang, Huai-Ting, 黃懷葶

博士 / 國立臺灣海洋大學 / 海洋生物研究所 / 103 / White spot syndrome virus (WSSV) is a large enveloped DNA virus, and it causes a serious disease that has led to severe mortalities of cultured shrimps in many countries. To determine the mechanism of virus entry into the cell and to establish an antiviral strategy, the cell receptor for virus entry and receptor binding protein should be determined. In this study, a surface protein, named glucose transporter 1 (Glut1), which could interact with WSSV envelope prote...

Hsieh, Jen-Fang, 謝仁方

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / Immunity parameters and the expression levels of immune-related proteins including lipopolysaccharide and β-glucan binding protein (LGBP), peroxinectin (PX), intergin β(IB), prophenoloxidase (proPO) I, proPO II, α2-macroglobulin (α2-M), cytosolic mangangese superoxide dismutase (cytMnSOD), mitochondria manganese superoxide dismutase (mtMnSOD), catalase, glutathione peroxidase (GPx), lysozyme, and penaeidin 3a were examined in white shrimp Litopenaeus vannamei reare...

Kao, Szu-Chia, 高似嘉

博士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 104 / The Sergia 1ucens and Pasiphaea japonica are the main species of the sergestid shrimp fisheries in the I-Lan Bay of northeastern Taiwan. In this study, a multidisciplinary observation was carried out on board “Ocean Research II” in I-Lan Bay in northeastern Taiwan, recording acoustic, biological, and hydrographic data. The 120 and 38 kHz acoustic volume backscattering strength (SV) data of Simrad EK60 echosounder were simultaneously collected with the 105 biol...

Liu, Pei-Wen, 劉姵妏

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / This study investigated the pathogenicity and virulence factors of Vibrio strains Lvhpvpa、VnLvvpa 2、Abvpa1 and HlLvvpa isolated from white shrimp (Litopenaeus vannamei) and abalone (Haliotis diversicolor supertexta). After detection of biochemical characteristics and specific genes etc, these Vibrio strains were confirmed to be Vibrio parahaemolyticus. In addition, after checking by the presence of specific genes of AHPND strain, Lvhpvpa and Abvpa1 were identified ...

Chiu-Hsia Li, 李秋霞

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / This study monitored the immune response of white shrimp Litopenaeus vannamei ( 10.42 ± 1.39 g ) and its resistance against Vibrio alginolyticus when the shrimp were injected with hot-water extract of Sargassum duplicatum or Petalonia binghamiae at a dose of 2、6、10 or 20 μg g-1. The immune parameters included total haemocyte count (THC), phenoloxidase activity, respiratory burst (release of superoxide anion), superoxide dismutase activity, phagocytic activity and cl...

Yu-Wen Fu, 傅郁文

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / Abstract This study examined the immune responses of white shrimp Litpenaeus vannamei and its resistance against Vibrio alginolyticus when the shrimp were fed diets containing the hot-water extract of Gracilaria tenuistipitata and Gelidium amansii at 0、0.5、1.0及2.0 g kg-1. The immune parameters included total haemocyte count (THC)、different haemocyte count (DHC)、phenoloxidase activity、respiratory burst (release of superoxide anion)、superoxide dismutase (SOD) act...

Ching-Hsu Chang, 張清旭

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / The purpose of this thesis fed diets with 2, 8 and 32 ppm of cortisone and 20-hydroxyecdysone during the 30 days culture period. To know the effects of dietary steroid hormones on PO activity and O2- production in the haemocytes, total haemocyte count (THC), Superoxide dismutase (SOD) activity in the hepatopancreas, Glutamate oxidacetate transaminase activity (GOT), Glutamate pyruvate transaminase activity (GPT), and plasma Cholesterol, Triglycerides, Total protein...

Chun-Hung Liu, 劉俊宏

博士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / Abstract Shrimp disease caused serious economic losses in world shrimp culture industry. These may be relevant in the control of infectious disease in imtensive farming of economically important shrimp. However, it is important to understand the immune mechanism of shrimp for prevent disease. In this project, white shrimp Litopenaeus vannamei was selected as a model species. The study is to focus on the cell adhesion protein, peroxinectin, which regulates and ampli...

Chia-Wei Hsiao, 蕭嘉韋

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / Abstract Two experiments were conducted to investigate the effects of K-diformate on the weight gain, survival and chemical compositions of muscle and liver of cobia, Rachycentron canadum. In the first trial, triplicate groups of cobia (6.9±0.1g initial body weight) were fed diets including four supplemental K-diformate levels (0, 0.5, 1 and 1.5 g/kg). Fish meal was used as a dietary protein and the mixture of 2:1 cod liver oil/corn oil used as a dietary lipid. No...

Tseng, Kuei-Chi, 曾貴祺

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 102 / White shrimp Litopenaeus vannamei is a important species of aquaculture in the world. White shrimp juveniles (0.6 ± 0.05 g) which had been reared at different pH leavel (pH 6.8、8.1、9.2) for 24 weeks were examined for the survival, growth, immune activation, immune parameter, resistance against Vibro alginolyticus and expressions of lipopolysacchairde-β-glucan binding protein (LGBP), C-type lectin, peroxinectin (PX), integrin β, serine proteinase, prophenoloxidase　(...

Po-Yuan Chang, 張博淵

碩士 / 嘉南藥理科技大學 / 生物科技系 / 101 / WSSV is a new large double-stranded DNA virus that infects aquatic crustaceans, and causes damages and losses to cultured shrimp. The around 300-kb genome of WSSV Taiwan isolate ( WSSV-TW ) encodes 532 putative open reading frames ( ORFs ). Some of them have been characterized encoding structural proteins of WSSV virions and many nonstructural proteins transiently expression during virus infection. It is study, time-course study using by real-time PCR showed that th...

Nian-Zu Li, 李念祖

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / The marketing price of atydae shrimp(cherry shrimp, Neocaridina denticulata) is greatly influenced by its pigmentation, which is directly influenced by changes in chromatophores.In this thesis, effects of background lightness, hue, illumination period and wavelength on the pigmentation of chromatophores in atydae shrimp are investigated, which include:I. The effect of background lightness on the state of chromatophores and pigmentation of atydae shrimp under three t...

Kuan-Fu Liu, 劉冠甫

博士 / 國立臺灣大學 / 動物學研究所 / 99 /

Zhi-Yong yang, 楊志勇

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 99 / White spot syndrome virus (WSSV) is the viral pathogen of shrimp white spot disease and causes large economic losses to global shrimp farming. In this study, Escherichia coli system was chose to express two WSSV envelope proteins, VP28 and VP36B, inoculated white shrimp (Litopenaeus vannamei) as subunit vaccine. Shrimps were divided into 3 groups and intramuscularly injected rVP28, rVP36B or PBS two times at intervals of 3 days. After vaccination, sampling of h...

SUN, XIONG-CAI, 孫雄材

碩士 / 國立中興大學 / 食品科技研究所 / 74 /

Chiou, Saw Hau, 邱少華

碩士 / 大葉工學院 / 食品工程研究所 / 85 /

楊孟琳

碩士 / 國立海洋大學 / 食品科學系 / 90 /

Tseng I Ting, 曾怡婷

碩士 / 國立海洋大學 / 水產養殖學系 / 90 / Abstract This paper deals with the effects of intrinsic factors like gender, size, molt stages and feeding rate, and the effect of extrinsic factors like ammonia, nitrite and copper sulfate on the immune response of Litopenaeus vannamei. Immune tools used are total haemocyte count (THC), hyaline cell, granular cell, phenoloxidase, respiratory burst and superoxide dismutase (SOD). L. vannamei challenged with Vibrio alginolyticus were placed in water containg...

Jeng,Jia-Yu, 鄭嘉裕

碩士 / 國立高雄海洋科技大學 / 漁業生產與管理研究所 / 97 / 1. Low temperature is the main cause affecting milkfish(Chanos chanos) cultivation. Ten tests of temperature reduction have been done repeatedly in this research from 25℃ to 11℃. At 16℃, the whole group of milkfish drops to the pool bottom and one of them dies. At 15.0℃, 2% of them die. At 14.5℃, 10% die. At 13℃, 100% die. As a result, 13℃ is the fatal temperature for milkfish. 2. Output of single milkfish cultivation in Taiwan is 36,500 tons. Total stoc...

Wen-Chih Chang, 張文智

博士 / 國立中興大學 / 食品科學系 / 91 / The production of inexpensive antifungal hydrolytic enzymes is an element in the utilization of shellfish processing wastes. In this study, shrimp and crab shell powder prepared by treating shrimp and crab processing wastes with boiling and crushing was used as a substrate for the culturing of an antifungal chitinase producing microorganism. Bacillus cereus YQ308 produced antifungal hydrolytic enzymes, including chitinase, chitosanase and protease, when it was grown in...

Yu-Chen Yuan, 袁又宸

博士 / 國立臺灣大學 / 動物學研究所 / 92 /

Shao-En Peng, 彭紹恩

博士 / 國立臺灣大學 / 動物學研究所 / 88 / Investigation of the host range of WSSV with the highly sensitive and specific tool of WSSV diagnostic PCR revealed that WSSV could be detected in cultured shrimp (Penaeus monodon, Marsupenaeus japonicus, Fenneropenaeus penicillatus, Metapenaeus ensis, P. semisulcatus, Litopenaeus vannamei) and crab (Scylla serrata). It also confirmed that WSSV exists in wild-caught shrimp (P. monodon, M. japonicus, F. penicillatus, M. ensis, P. semisulcatus) and crabs (Portunus sangu...

Li-Hao Chen, 陳力豪

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 100 / Shrimp white spot syndrome virus (WSSV) is one of the most devastating viral pathogens of cultured shrimp worldwide. Although adaptive immunity is believed to exist only in higher vertebrates, recent published papers showed the ability of WSSV structural protein VP28 to vaccinate shrimp and other crustaceans against WSSV. This study attempted to identify the joining proteins of the mentioned shrimp quasi-immune response at the translational level by proteomic ...

Shu, Chung Wan, 徐崇婉

碩士 / 國立海洋大學 / 水產食品科學系 / 85 /

Yu, Yi-Ping, 游宜屏

碩士 / 國立海洋大學 / 水產食品科學系 / 85 /

Sun Ryan, 孫咸義

碩士 / 東吳大學 / 微生物學系 / 86 / In the crustaceans, the nonspecific immune response is the primary defense system and the cellular immunity is more important. To study and clarify the roles of different haemocytes in cellular immune responses, separation and classification of haemocytes are essential. Four monoclonal antibodies, including Z5E10、Z6A5、Z6A6 and Z6H8, developed in our laboratory are specific binding to the different haemocyte types of tiger shrimp (Penaeus monodon), respectively.In this st...

LIN, ZHE-LI, 林哲立

碩士 / 國立臺灣大學 / 生化科學研究所 / 80 /

Teng-ChunTung, 董騰駿

碩士 / 國立成功大學 / 生物科技與產業科學系 / 107

Ting Yang, 楊庭

碩士 / 國立宜蘭大學 / 生物機電工程學系碩士班 / 105 / The objective of this research was to develop a remote monitoring APP implemented in a light-weight shrimp peeler. The light-weight shrimp peeler can be divided into five units: shelling, squeezing, cleaning, driving and discharging unit. The control parameters of the best peeling rate were obtained by the three kinds of shrimp in Whiteleg shrimp, Mino Nylon shrimp and Spear Littoral shrimp. In line with the development trend of Internet of Things and Industry 4...

CHEN, JHENG-SIOU, 陳正修

碩士 / 國立高雄科技大學 / 企業管理系 / 107 / In the past, numerous studies have examined the effect of service quality on repurchase intentions. However, they have not examined the effect mentioned above in the context of the fast fried restaurant. This study aims to explore the impact of service quality on repurchase intentions and perceived value is set as the mediating variable. The customers having visited Mr. Shrimp Restaurant are the subjects for the empirical study. The total sample size was 200. Using ...

Chi, Chia-Chun, 紀佳君

碩士 / 國立屏東科技大學 / 水產養殖系所 / 106 / This study examined the mechanisms of action of Bacillus subtilis E20 in activating the immunity of shrimp via dietary administration. The white shrimp were firstly divided into two groups, one group was fed a control diet (a basal diet) and the other fed an E20-containing diet (a basal diet with 109 CFU kg-1 of B. subtilis E20 added). After the 8-week feeding regimen, the composition of hepatopancreas metabolite in shrimp were investiagted using 1H nuclear magneti...

Huang, Chun-Jen, 黃群仁

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 106 / This study aims to investigate the effects of immunostimulants (PROMIX Q5) on non-specific immune responses in white shrimp (Litopeneaus vannamei). Total haemocyte count (THC), phagocytic rate (PR), phagocytic index (PI), superoxide anion production (O2-), phenoloxidase activity (PO), phenoloxidase gene expression were determined as the indicators of immune activity. Experiment I: shrimp haemocytes were cultured with different concentrations (0, 0.1, 0.3, 0.5, 1, 3...

Chung-Ming Chunh, 鍾傑名

博士 / 義守大學 / 資訊工程學系 / 106 / In order to avoid the marine’s products face global exhausted and extinct, and reduce the neediness for ensuring the quality of fishes, those are the main purposes of monitor the quality of fishes. If we can preserve properly after the consumption of marine products for a period, it can be extending the usage period of the marine industry and improve the validity period of it. In the face of customization of the global fishing market, demands of customers grow stably, ...

Huang, Yu-Kai, 黃俞凱

碩士 / 國立屏東科技大學 / 資訊管理系所 / 105 / The pet markets develop rapidly in the world, the pet market ranking third is ornamental fish market, only next to the dog and cat market. According to the data of the Food and Agriculture Organization (FAO), the peripheral products of ornamental fish industry and aquarium equipments promote the gross output value to as high as US$15 billion. According to the statistics of the Ornamental Fish Association, Taiwan has exported 18 million ornamental shrimps annually s...

Che-ChihChang, 張哲輊

碩士 / 國立成功大學 / 生物科技研究所 / 105

Hu, Jia-Yu, 胡家瑜

碩士 / 國立臺灣海洋大學 / 食品科學系 / 106 / Vibrio parahaemolyticus was reported as the major pathogen leading to foodborne outbreaks in Taiwan. Microbial predictive model could be rapidly to describe the growth behavior of V. parahaemolyticus in different temperature level. However, present study of development a specific predictive model in conection with V. parahaemolyticus strains in Taiwan is still few. Thus, this study aim to develop and validate the predictive growth model of pathogenic strains of V. p...

Cheng, Ya-Chi, 鄭雅琪

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 106 / The study was conducted to investigate the effects of dietary supplement of grape extract on the growth performance and lipid peroxidation of white shrimp, Litopenaeus vannamei. 　　The effects of grape (Nor-grape 80), polyphenol-rich feed additive, on the growth performance, immune parameters and lipid peroxidation of 0.02 g white shrimp Litopenaeus vannamei were investigated in experiment I and II. Four experimental diets included 50 (N50), 100 (N100), 150 (N150) a...

Sun, Po-Wei, 孫博威

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 106 / This experiment compares the effects of different feed additives at different time on superoxide dismutase (SOD), glutathione peroxidase (GPx), blood biochemical parameters and growth parameters of white shrimp. There are nine groups of experiment feeds with different additives which are commercial (A), commercial (B), commercial (C), lipoic acid (E), astaxanthin (F), commercial (G), commercial (H), Ganoderma (I), and the control (D), respectively. Triplicated test...

Shi-Wei Lin, 林世偉

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 102 / We have performed the comparative proteomic research which based on the two-dimensional gel electrophoresis and mass spectrometry (2-DE/MS), it is found that the altered level of some identified proteins in shrimp, Litopenaeus vannamei, following a protein-based vaccination might be responsible for conferring resistance against White Spot Syndrome Virus (WSSV). In this study, after the proteomic analysis, the mRNA expression of six chosen proteins included Cal...

Tang, Wei-Chi, 唐偉琦

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 107 / Core Binding Factor (CBF), a heterodimer composed of a DNA binding α-subunit and a non-DNA binding β subunit, plays a vital role in hematopoiesis regulation. We had identified the Litopenaeus vannamei -subunit gene, L. vannamei lozenge (Lvlz), and had a preliminary research on it. This study then aimed at unveiling the possible role of L. vannamei -subunit gene, L. vannamei CBFLvCBF, in shrimp hematopoiesis. We had identified from our Lv hemocyte transcript...

Shih-Ting Kang, 康詩婷

碩士 / 國立臺灣大學 / 動物學研究研究所 / 93 / WSSV396, WSSV395 and WSSV394 are identified in pervious research as the three of thirty-nine structural proteins of shrimp white spot syndrome virus (WSSV). This study focuses on the transcriptional and translational analysis of wssv396, wssv395 and wssv394. The 3’RACE results reveal that wssv396, wssv395, and wssv394 share the same polyA tail. A common ~3.4-kb transcript, which is consistent with the predicted size of a polycistronic transcript encoding these three...

Yu,Yan-Bin, 余彥彬

碩士 / 國立屏東科技大學 / 農企業管理系所 / 103 / Sakura shrimps are precious fishery resources in very few countries worldwide, and they have become one of the important fishery industries in Taiwan. The data from Donggang auction market showed that the average price per case of sakura shrimps has increased from 450 NTD in 1992 to around 5,000 NTD in 2014, and they brought 4.78 billion NTD of total revenues to fishermen. Given that the sakura shrimp producaiton and marketing group in Donggang used the inherent a...

ZHENG,MING-XIU, 鄭明修

博士 / 國立臺灣大學 / 海洋研究所 / 79 /

Chen, Xiang-Yin, 陳香吟

碩士 / 國立臺灣大學 / 食品科技研究所 / 75 /

張鈺驩

碩士 / 國立臺灣大學 / 農業化學研究所 / 75 /