魏榮璋

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / The investigation was conducted to study the properties and pathogenesis of Aeromons hydrophila which was isolated from disease fresh water prawn(Macrobrachium rosenbergii). Three strains,860312,860327and870121 used in study were isolated from Ping-Tong,southern Taiwan.These strains were identified to be Aeromons hydrophila-like according to a series of biochemical tests using API 20E kits、BIOLOG GN system,and biochemical plate and tube tests.The reactio...

Wen-Bin Kao, 高文彬

碩士 / 國立清華大學 / 生命科學系 / 89 / At the start of the 20th century, scientists have hypothesized that learning and memory could be encoded in the strength of the synaptic connections between neu- rons（Tanzi, E., 1893）. In 1970s, scientists reported that high-frequency stimulation input to the dentate gyrus produced a long-lasting enhancement of field potential (Bliss and Lomo, 1973), they called long-term potentiation(LTP). Most of the LTP phenomens are related to the glutamate receptors. NMDA recepto...

Wen-Chi Wei, 魏紋祈

碩士 / 國立中山大學 / 海洋資源學系研究所 / 89 / Abstract Recent researches have pointed out that most of marine bacteria are uncultivable. However, majority of prior researches about bacteria flora in cultivated ponds used cultivating method to researches. That means those researches ignored uncultivable bacteria in ponds and caused inaccuracy in counting bacteria. Therefore we used analyzing bacteria 16S rDNA sequences to study composition of bacteria in cultivated ponds in place of traditional bacteria taxonom...

Chi-Hsiang Hung, 洪基翔

碩士 / 國立中山大學 / 海洋生物研究所 / 89 / This study aims to elucidate the process and mechanism of sperm penetration through eggs of Penaeus monodon. Sperm penetration of the vitelline envelopes (VEs) of P. monodon eggs were observed with the scanning electron microscope. The characteristics of sperm proteases in the sperm extracts from seminal receptacles of females were analysized. In P. monodon, mating and sperm transfer to the thelycum of female occur soon after maturity moult. Females store the sperm i...

Xu, Jin-Rong, 許晉榮

碩士 / 國立臺灣大學 / 漁業科學研究所 / 81 /

Yuen-Fen Chang, 張月芬

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 100 / This study observed the changes on grill, hepatopancreas, and midgut while shrimp (Litopenaeus vannamei) exposed to copper (0.5 and 1 ppm) and zinc (0.5 and 1 ppm) environment with exposure time (1, 2 and 5 days). The results show the grill and hepatopancreas had significant damage while shrimps exposed at the copper solution. The shrimps exposed at the zinc solution, the midgut had significant damage than the other tissues. But shrimp exposed at solution that...

Jia-HungLin, 林家弘

碩士 / 國立成功大學 / 生物科技研究所 / 102 / Until now, the mechanism of shrimp immune response still not fully understood. Previously, we found that shrimp has a hypervariable molecule called Down syndrome cell adhesion molecule (Dscam) which can recognize different pathogens. Based on Dscam structure and immune properties, Dscam has been proposed to be an antibody-like molecule in arthropod. In this study we use white spot syndrome virus (WSSV) as pathogen stimulator to observe the Dscam’s cytoplasmic tail v...

CHUANG KAI CHUAN, 莊凱荃

碩士 / 逢甲大學 / 化學工程學系 / 102 / Haematococcus pluvialis contain with abundant natural astaxanthin, which is full of antioxidant capacity that can quench free radicals, improve immune system, and prevent aging and cancer. Up to now, natural astaxanthin has been discovered as a most powerful antioxidant in natural resources. In this study, it’s divided into two steps to cultivate Haematococcus pluvialis. Before the first step, we found that replacing fresh medium regularly can nurture Haematococcus plu...

Ming-Hung Huang, 黃明宏

碩士 / 嘉南藥理科技大學 / 生物科技系暨研究所 / 93 / White Spot Syndrome Virus (WSSV) is a large, circular dsDNA virus , which may infect shrimps and other crustaceans. This virus is a member of the genus Whispovirus within a new virus family called Nimaviridae, referred to the thread-like polar extension on the virus particle. The complete sequences of the WSSV genomes have now been published as three different isolates. However, most of WSSV open reading frames , which can encode proteins, have no homology to a...

Miao-Hsien Chen, 陳妙嫻

碩士 / 國立臺灣大學 / 生態學與演化生物學研究所 / 94 / Information on habitat use can enhance our understanding to a species’ecological requirements and physiological costraints, then we can make relevant suggestions about conservation and management. Neocaridina denticulata is a very common shrimp speices in Taiwan streams. They play an important role in freshwater ecosystem and an economical species in aquaculture. To understand hibitat use of Neocaridina denticulata, we use electric fishing method to capture shri...

Tai-Jung Wu, 吳岱融

碩士 / 國立臺灣大學 / 動物學研究研究所 / 94 / In this study, we established a high throughput and prompt procedure to purify the native form of ribosomes from Penaeus monodon stomach tissues by means of two-steps ultracentrifugation and discontinuous sucrose density phase. Using Transmission Electron Microscope (TEM) to observe purified protein samples, we discovered the polysome structure in it. These ribosomal proteins were separated by 17.5 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAG...

葉鳳枝

碩士 / 國立彰化師範大學 / 生物學系 / 91 / In crustaceans, circulating hemocytes assume various immune functions. The present study determined the presence and biochemical characteristics of nitric oxide synthase (NOS) and investigated the immune roles of nitric oxide (NO) in the hemocytes of the crayfish Procambarus clarkii. Morphological analysis identified three types of hemocytes, namely, hyaline, semigranular, and granular cells. Biochemical analysis indicated the presence of a Ca2+-independent NOS in the...

Chen, Ying, 陳穎

碩士 / 國立海洋大學 / 水產食品科學系 / 86 /

PAN,ZHI-HONG, 潘志弘

碩士 / 國立海洋大學 / 漁業研究所 / 78 /

CHEN,QIU-JIN, 陳秋錦

碩士 / 國立海洋大學 / 水產養殖研究所 / 78 /

HUANG,YONG-SEN, 黃永森

碩士 / 國立海洋大學 / 水產養殖研究所 / 78 /

GUO,YAO-REN, 郭曜任

碩士 / 國立海洋大學 / 水產養殖研究所 / 78 /

CHEN,XUE-FEN, 陳雪芬

碩士 / 國立海洋大學 / 漁業研究所 / 79 /

LI,FANG-YI, 李芳儀

碩士 / 國立海洋大學 / 水產養殖研究所 / 79 /

ZHANG, CONG-ZHOU, 張聰洲

碩士 / 國立中興大學 / 獸醫研究所 / 75 /

XIE , JIE-SHI, 謝介士

碩士 / 國立臺灣大學 / 海洋研究所 / 74 /

Chien-Hua Chiu, 邱建樺

碩士 / 國立臺灣大學 / 動物學研究所 / 89 /

Zhao-June Chen, 陳昭君

碩士 / 國立臺灣大學 / 動物學研究所 / 89 / The goal of this study is to establish a Capture enzyme-linked immunosorbent assay (ELISA) system for the quantification assay of the whitespot syndrome virus (WSSV). This system is designed as that a monoclonal antibody recognizing a major viral envelope protein, VP 28, was used as the primary antibody and the polyclonal antibodies affinity-purified from WSSV-immunized rats sera as the secondary antibody. First, a standard curve was plotted from purified WSSV contain...

陳亮洲

碩士 / 國立海洋大學 / 水產養殖學系 / 91 / This study investigated the virulence of Vibrio alginolyticus strain 9206 isolated from diseased white shrimp (Litopenaeus vannamei) grown at various temperature and concentration of NaCl. Virulence of the bacteria cells of V. carchariae (strain 9211) or V. paraheamolyticus (strain 9301) in white shrimp disease was also conducted for comparsion. The LD50 values of V. alginolyticus (strain 9206), V. carchariae (strain 9211) and V. paraheamolyticus (strain 93...

Huang, Xie-Tian, 黃謝田

碩士 / 國立海洋大學 / 水產養殖研究所 / 81 /

Kuo-Ming Yang, 楊國明

碩士 / 國立海洋大學 / 漁業學系 / 81 /

Huang, Shieh-Tyan, 黃謝田

碩士 / 國立海洋大學 / 水產養殖學系 / 81 /

Jeng, Shan Ru, 鄭絢如

碩士 / 國立海洋大學 / 水產養殖學系 / 81 /

XU, RONG-BIN, 徐榮彬

碩士 / 國立中興大學 / 獸醫研究所 / 81 /

Shi, Zhi Yun, 施志昀

博士 / 國立海洋大學 / 漁業科學研究所 / 82 /

Huang, Qian Fang, 黃千芳

碩士 / 國立海洋大學 / 海洋生物研究所 / 82 /

Lu, Wen Jie, 盧文杰

碩士 / 國立海洋大學 / 水產養殖研究所 / 82 /

Chen, I Shiung, 陳義雄

碩士 / 國立中山大學 / 海洋資源學系 / 82 / The gobies of genus Rhinogobius inhabit in streams, rivers ,lakes and estuaries of East Asia region which contain both land-lock and diadromous type of life histories. In Taiwan, they arethe most dominant group of benthic fish fauna in the freshwater and brackish waters. But only a few papers has been published their systematics or ecological studies. Thus, the purpose ofthis stu...

Wur, Fehng Your, 吳鳳酉

碩士 / 國立中山大學 / 海洋生物研究所 / 82 / Three experiments were conducted in FRP tanks with artificial shelters to study the effect of shelters on Penaeus monodon. In one experiment, distribution of P. monodon on artificial shelters was examined. Distribution of P. monodon in horizontal shelter changed with time of day. It tends to stay on the bottom in the morning and at noon but spreaded to the ot...

Chou, Wei Jung, 周偉融

碩士 / 國立中山大學 / 海洋生物研究所 / 82 / The present research investigated the absorption and metabolism of L-[1-13C]methionine in Penaeus monodon to understand the reasons why the prawn can not efficiently utilize crystalline amino acids supplemented in diet.Two experiments were conducted. The first experiment studied the change of L-[1-13C] methionine concentration in the hemolymph of Penaeus monodon (20- 30g) within...

Chen-Chu Chiang, 蔣真祝

碩士 / 國立宜蘭大學 / 生物資源學院碩士在職專班 / 104 / Astaxanthin is one kind of carotenoid and it is the strongest antioxidant activity among the natural materials. Haematococcus pluvialis has the highest astaxanthin content. However, H. pluvialis has a thick cell wall, which causes resistance of solvent extraction of astaxanthin. The objectives of this study were to establish extraction and analysis methods for astaxanthin from H. pluvialis, and to analyze the antioxidant activity and stability of extract. Final...

Shih-Hung Chang, 張世弘

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 96 / Brine shrimp nauplii (Artemia sp.) are mostly used as the livefeed for many cultured marine shellfish and finfish larvae, and it has also been used in the biological study as a model organism. Yet, the transgenesis of artemia has not well documented. Here, we applied electroporation on artemia cysts to generate the transgenic artemia. After the artemia cysts were decapsulated, the cysts were then electroporated with two plasmids, in which one contained GFP report...

Yen-Hsun Chen, 陳彥勳

碩士 / 國立臺灣大學 / 漁業科學研究所 / 97 /

Yi-Ting Yang, 楊怡婷

博士 / 國立臺灣大學 / 生命科學系 / 103 / Penaeus monodon nudivirus (PmNV) is the causative agent of spherical baculovirosis in shrimp (Penaeus monodon). This disease causes significant mortalities at the larval stage and early postlarval (PL) stage and may suppress growth and reduce survival and production in aquaculture. The nomenclature and classification status of PmNV has been changed several times due to morphological observation and phylogenetic analysis of its partial genome sequence. In this study, w...

Chen, Mei - Hui, 陳美慧

碩士 / 崑山科技大學 / 環境工程研究所 / 102 / Macrobrachium nipponense extensively inhabits rivers and lakes in Taiwan. In assisting resource evaluation and management, the study assessed the reproductive biological characteristic of M. nipponense by gathering samples in Sihchong River of Pingtung twice a month from March 2012 to May 2013. Within the specimens, 104 are spawning shrimps (N= 2,474). Average length (L) and weight (W) are 2.7 cm to 7.9 cm and 0.09 g to 2.58 g respectively with power function of W= ...

YANG, WAN-TING, 楊皖婷

碩士 / 國立臺北護理健康大學 / 運動保健研究所 / 105 / According to related researches, the supplement of astaxanthin may help improving the power of exercise. The study intends to understand the effect of astaxanthin supplement to maximum VO2max, exhaustion time, VE, failure heart rate and LA after exercising of sport performance, affection to their improvement or promotion. The double-blind experiment design is adopted by the study. 14 healthy males aged 20-40 were the subjects and dividend into astaxanthin and co...

I-Hao Chan, 詹益豪

碩士 / 國立中山大學 / 機械與機電工程學系研究所 / 91 / This proposal is about a project to study the biological activities of Artemia caused by ultrasound exposure. Ultrasound is employed clinically, for example, in medical diagnosis as a pulse-echo technique for obtaining information of tissue characteristics. It can also use the high-intensity-ultrasound to destroy the lump and pathogens of human tissues. In the other hand, the ultrasonic experiments of plant or insect tissues that contain gas in intercellular cha...

Ping-Chung, Liu, 劉秉忠

博士 / 國立臺灣大學 / 動物學系 / 84 /

Wang, Kai Hong, 王凱弘

碩士 / 國立海洋大學 / 生物技術研究所 / 84 /

Lu, Chow-Chin, 盧秀琴

博士 / 國立臺灣大學 / 動物學系 / 82 / Penaeus monodon-type baculovirus (MBV) was isolated and purified from the hepatopancreata of MBV- infected Penaeus monodon Fabricius. MBV DNA was extracted and used as a template in polymerase chain reaction (PCR). The primers were chosen from conserved regions of the polyhedrin gene of Autographa califor- nica nuclear polyhedrosis virus (AcNPV). One DNA fragment (674 base pairs)...

Fung, Wai-Chio, 馮偉超

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 103 / 專利申請中

Long Chi Chen, 陳隆吉

碩士 / 國立屏東科技大學 / 水產養殖系 / 91 / To investigate the current aspects of freshwater giant prawn, Macrobrachium rosenbergii, cultured in Taiwan, a three-experiment monoculture system was carried out and all items of cultural parameters were also collected. Three trials with an area of 1) 0.7 ha area with one 0.1 ha pond plus two 0.3 ha ponds, 2) 0.7 ha area with one 0.3 ha pond and one 0.4 ha pond, and 3) 1.0 ha area with one 0.4 ha pond and two 0.3 ha ponds. The raising periods were 357, 224 and 388 d...

Hsu-Tung Yi, 徐東儀

碩士 / 國立屏東科技大學 / 水產養殖系 / 94 / This present study profiles the physiological metabolism of giant freshwater prawn (Macrobrachium rosenbergii) infected with yeast (Debaromyces hansenii). The relationship between the concentration of yeast in prawn hemolymph and physiological parameters, which including hemolymph osmolality, sodium, potassium, chloride, calcium, magnesium, ammonia, glucose, lactate, partial pressure of oxygen (pO2), partial pressure of carbon dioxide (pCO2), acid-base balance and th...

SHIH,HUEI-YUN, 石惠韻

碩士 / 崑山科技大學 / 環境工程研究所 / 104 / The Austinogebia edulis Ngo-Ho and Chan, 1992, also named "Luk-Kong mud-shrimp " is the aquatic delicacy for the inhabitants of Changhua Coastal since ancient times. However,due to the vandalism such as the changes of capture mode,the waterfront development and the establishment of industrial zones in recent years,the inhabitat environment for the coastal creatures has significantly reduced,leading A. edulis is about to disappear in Changhua. However,the traces of A...

Lu, Kuan-Chieh, 呂冠杰

碩士 / 國立交通大學 / 生物資訊及系統生物研究所 / 103 / Neocaridina denticulata, known as black cherry shrimps, has been as test subjects in Taiwan for water toxicity texting experiments for its tribute tendency to response to a variety of chronic or acute toxicity. However, both the genomic and transcriptomic information of such an organism is limited. To further improve the current experiment design and elucidate the mechanism of Neocaridina denticulata’s response to pollutants, we reconstructed the transcriptome ...