Jhih-Jyun He, 賀之軍

碩士 / 國立宜蘭大學 / 生物技術與動物科學系 / 103

Ke, Jia-teng, 柯家騰

碩士 / 國立屏東科技大學 / 水產養殖系所 / 104 / Dopamine (DA) was found to influence the immunological responses and resistance to pathogen infection in invertebrates. To clarify the possible modulation of DA through dopamine receptor (DAR) against acute environmental stress, the levels of DA, glucose and lactate in the haemolymph of Macrobrachium rosenbergii under hypo- (15 °C) and hyperthermal (34 °C) stresses were measured. Prawn received different dopamine receptor antagonists, such as SCH23390 (D1 DAR antag...

Guo-Jun Liao, 廖國均

碩士 / 弘光科技大學 / 生物科技研究所 / 104 / Abstract This research paper using Homocysteine and Glutamate induced cytotoxicity of PC12 cell line, and investigate the Astaxanthin effect of their protection.In humans, Homocysteine is a metabolic intermediate of methionine. Adverse metabolic effects have high Homocysteine concentrations. Homocysteine is easily oxidized to form reactive oxygen species, generated cytotoxicity.Or through a series of reaction to Homocysteine thiolactone, leading to protein misf...

蕭舜如

摘　要 電子商務行動化已變成趨勢，而消費者個人間的C2C交易市場也越來越受到注目，尤其是讓使用者輕鬆上架商品、簡化繁複流程的新型態行動拍賣App，更是熱門議題。本研究欲了解台灣目前C2C行動拍賣App中，最具話題性及代表性的「蝦皮拍賣」之顧客體驗流程，研究方法採半結構式深度訪談法，以使用網路購物經驗超過五年以上且使用蝦皮經驗滿三個月以上、同時具有買家跟賣家雙重身分之使用者為研究對象。 本研究主要發現如下：1.使用蝦皮的正向使用經驗在於有清楚直觀好用的使用者介面、擁有即時性及雙向互動並保有使用者隱私性的即時通訊「聊聊」功能、採用可降低風險的第三方支付、因免運費帶起的方便性物流選擇-超商寄貨取貨、讓賣家寄件步驟簡化的整合寄件物流以及使資訊透明的物流追蹤。2.初次使用動機則是受到免運費吸引及社群口碑推薦影響。3.持續使用因素除了涵蓋正向使用經驗因素外，還包括常有免運費或運費補助的促銷活動、同時身兼買賣家身分、因平臺上商品及賣場多而選擇性高及免費的商品置頂推廣功能。4.蝦皮的待改進項目則在於商品上架過程從簡易變複雜、評價多為制式評價因此參考價值有限、推播通知字數多易遭使用者忽略。本研究並且透過...

Su, Liang-Ju, 蘇亮如

碩士 / 國立屏東科技大學 / 動物科學與畜產系所 / 104 / The trial was conducted to study the effects of dietary astaxanthin (AST) supplementation on laying performance, egg quality, and yolk characteristics of laying hens. A total of ninety Leghorn layers at 36 weeks of age were randomly assigned into 5 groups according to the egg production rate. Each group of hens had three replicates with 6 layers. A corn-soybean meal basal diet was offered in the control group; 4 experimental diets were added with 50, 100 and 500...

林文祈

碩士 / 佛光大學 / 學習與數位科技學系 / 99 / By introducing Boshiamy input method to the fifth and sixth graders, this study tried to solve the problem of students’ Chinese character recognition. The participants of this action research were the 28 fifth and sixth graders in the researcher’s class. By using the Boshiamy input method practice program, the students involved in a two-semester instruction course. While the first stage focused on the instruction of Boshiamy input method, the second stage focused on ...

Chao-Yu Chen, 陳朝煜

碩士 / 輔仁大學 / 生命科學系碩士班 / 104 / White spot syndrome virus (WSSV) is a serious pathogen that can infect almost all crustaceans, like penaeid shrimp, lobsters, and crabs. After infection, the mortality typically reaches 100% within 3 to 10 days and caused great economic losses. The horizontal infection of WSSV may be between the penaeid shrimp by contacting or eating with the infected shrimp or ingesting some crustacea organism which carry WSSV. It was confirmed that the crustacean copepods can serve...

Lin,Si-Wei, 林思維

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 102 / Taiwan, a country renowned for its world-leading production of tiger prawns, has unfortunately been struck with white spot syndrome (WSS), severely reducing production. As the disease is currently incurable, developing a vaccine has become a top priority. This research sought to use probiotics to transport the envelope protein VP28 of the white spot syndrome virus (WSSV) as a type of vaccine. First, the characteristics of Pseudomonas sp. were analyzed, and the...

DAI,NING, 戴寧

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 104 / The outbreak of acute hepatopancreatic necrosis disease (AHPND) from 2009 has caused mass losses in shrimp farms worldwide. The strain of Vibrio parahaemolyticus which obtain a plasmid and secret toxins has been determined as the infectious agent. PCR methods were published and used to perform diagnosis in laboratory. This study developed methods of Loop-mediated isothermal amplification (LAMP) and Recombinase polymerase amplification (RPA) to detect AHPND and...

Yi-Hua Chen, 陳怡樺

碩士 / 國立臺灣大學 / 漁業科學研究所 / 104 / Most of land-based fish farming are freshwater aquaculture. In 2014, land-based aquaculture fish production has accounted for 64% of the total harvest of aquaculture. The aquaculture patterns have gradually become more intensive. The waste water which contains excess nutrients is disposed into the river. It may causes biological and environmental damage. The purpose of this study is to reduce the negative effect of Macrobrachium rosenbergii aquaculture by water-savi...

Deng-Yu Tseng, 曾登裕

博士 / 國立臺灣大學 / 動物學研究所 / 91 / Abstract The reproductive functions and mechanisms regulating vitellogenesis in crustaceas have been the subjects of controversy. We have cloned another type of vitellogenin cDNA (Vg1) another than Vg2 being described previously in freshwater giant prawn, Macrobrachium rosenbergii, and proven that Vg1 and Vg2 are simultaneously expressed. The similarity of the freshwater giant prawn Vg1 and Vg2 is as high as 94%, but the partial sequence of Vg1 fragment...

Nien Wen-Hisu, 粘雯琇

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / There were three experiments in this study. Experiment I was conducted to evaluate the levels of dietary fish oil/corn oil(2:1, w/w) mixture needed for maximum weight gain of juvenile Litopeaneus vannamei. Seven isoengergetic and isonitrogenous semi-purified diets with supplementary lipid levels ranging from 0 to 12% in 2% increments were evaluated with nearly metamorphosed post-larvae. White shrimp fed with diet without the oil supplement had significantly lower we...

Yi-Yu Lee, 李宜諭

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / Methyl farnesoate (MF), an analogue of the insect juvenile hormone III, has been implicated to play a vital role in the regulation of the growth and reproductive development in crustaceans. Farnesoic acid O-methyl transferase (FAMeT) is the key enzyme involved in catalyzing the final step in the MF biosynthetic pathway. In this studies, we report the cloning and characterization of the cDNA encoding the deduced FAMeT of the tiger shrimp (P. monodon) and white shrimp...

Yu-chih Yeh, 葉昱志

碩士 / 東吳大學 / 微生物學系 / 96 / Nonylphenol (NP) belongs to one group of endorcine disrupting chemicals (EDCs). NP effects on procreation, nerve, immunity and survival through accumulating in the organism. The aim of this study was to investigate the effects of NP on susceptibility of pathogen, eight cellular genes expression and sensitivity of giant freshwater prawn (Macrobrachium rosenbergii) via oral treatment. The results of susceptibility showed that 50% of shrimps died after the shrimps has been ...

林湘婷

碩士 / 弘光科技大學 / 生物科技研究所 / 100 / In the present study, we aimed at developing liposomal delivery system for Astaxanthin (Ast) targeting in ameliorating lipopolysaccharide (LPS)-induced hepatic and renal damages. We hypothesized that encapsulation of Ast in liposomes might promote the therapeutic potential against experimental animals challenged with LPS. For the preparation of liposomal encapsulated Ast, the egg yolk phospholipids (EPC) and Ast were homogenized with high pressure homogenizer to for...

Hsin-YiHung, 洪欣宜

碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 100 / In arthropods, the Down syndrome cell adhesion molecule (Dscam) is believed to be involved in “specific immune priming”. The extracellular region of arthropod Dscam can generate a great number of isoforms via mutually exclusive alternative splicing. This variability has been shown to function as a neural wiring adaptor in neural wiring and an antigen-specific receptor in arthropod specific immune responses. In shrimp Dscam, in addition to the extracellular regio...

Shu-Han Yang, 楊舒涵

碩士 / 國立臺灣大學 / 生化科技學系 / 99 / White spot syndrome virus (WSSV) infection can cause a significant mortality to shrimp cultivation. Our previous study identified the first kruppel-like factor (PmKLF) in Penaeus monodon. Inhibition of PmKLF by double-stranded RNAs decreased the mortality in WSSV-infected shrimps. According to genomic database, the sequences of P. monodon and Litopenaeus vanname display highly identity. In this study, a KLF from L. vannamei was cloned and characterized. The full-lengt...

Ping-Han Huang, 黃柄翰

碩士 / 國立臺灣大學 / 生化科技學系 / 99 / Sp1-like proteins and Kruppel-like factors (Sp/KLF) are highly related zincfinger proteins that have crucial roles in transcription. The characteristic feature of this fami- ly is the presence of three zinc fingers in the C-terminal domain, which bind to GC- box, CACCC-box and GT-box sequence, to mediate transcription. This study reports the cloning and characteristics of a Kruppel-like factor (PmKLF) from shrimp, Penae- usmonodon (PmKLF). The full-length PmKLF cDNA i...

Kun-Lin Li, 李昆霖

碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / Whiteleg shrimp (Litopenaeus vannamei), also known as Pacific white shrimp, is one of the major aquaculture species in Taiwan and around world, while the information of molecular regulation and basic gene knowledge on whiteleg shrimp are poor. In this study, we aim to apply Next-Generation Sequencing (NGS) technology to get the most updated and well annotated transcriptome of the Litopenaeus vannamei. Starting from 168 million illumina read pairs with average length...

Chen shi-chang, 陳世昌

碩士 / 國立高雄海洋科技大學 / 海洋環境工程研究所 / 98 / Estrogen-like and so on chemicals are the most being discussed kinds in the exogenous endocrine disrupter chemicals. These chemicals once enter water, which will easily cause biological illness to the creatures in the water, and what’s more, they may even cause decrease in the biological population so as to break the whole ecological balance. The research takes Macrobrachium nipponense that are the most in quantity and the most widespread in Taiwan freshwaters ...

Ying-Ru Lin, 林映如

碩士 / 國立臺灣大學 / 動物學研究所 / 98 / Abstract Water temperature is one of the most important factors of environment. High temperature (32-33℃) has been proven to reduce the mortality in WSSV infected shrimps, but the mechanism still remains unclear. In previous researches, two-dimensional gel electrophoresis analysis revealed two proteins, NAD-dependent aldehyde dehydrogenases (ALDH) and proteasome alpha 4 subunit that were markedly up-regulated after WSSV infected shrimps at 32℃. Moreover, as referring ...

Yeah, Jau Chau, 葉志超

碩士 / 大葉工學院 / 食品工程研究所 / 85 /

Lin, Yong-Chin, 林永慶

碩士 / 國立海洋大學 / 水產養殖學系 / 90 / Abstract Litopenaeus vannamei juveniles ( 3.97 ±1.40 g ) were exposed to different concentrations of nitrite-N (nitrite as nitrigon), using the static renewal method at different salinity levels of 15 ppt, 25 ppt and 35 ppt at 18℃. The 24, 48, 72, 96, 120 and 144 h LC50 of nitrite-N on L. vannamei juveniles were 187.9, 144.2, 92.5, 76.5, 67.1 and 61.1 mg/l at 15 ppt；274.1, 244., 224.8, 178.3, 164.3 and 152.4 mg/l at 25 ppt；521.2, 423.9, 375.0, 321.7, 281.2 ...

Jueen-Ya Chiou, 邱俊雅

碩士 / 弘光科技大學 / 生物科技研究所 / 97 / Abstract The carotenoid pigment astaxanthin has important applications in the nutraceutical, cosmetics, food and feed industries. Astaxanthin is a strong coloring agent and a potent antioxidant .In this study we examined whether astaxanthin had a neuroprotection against H2O2-induced injury in PC12 cells. Calcium and lipid peroxidation play important roles in oxidative stress-induced cellular injury and apoptosis,which ultimately cause cell death. Pretreatment of PC12...

Hsin-ju Sung, 宋昕如

碩士 / 東吳大學 / 微生物學系 / 94 / In the studies of prawns, the expression pattern and regulation mechanism of a specific gene is more often being observed by means of injecting immunostimulants, while the changes of the entire gene expression pattern is less being noticed. In this study, Demethylated nucleic acid CpG ODN 2006 and the lipopolysaccharide (LPS) of the Gram-Negative bacteria cell wall were the two immunostimulants used to inject into freshwater prawn Macrobranchium rosenbergii. The hemocyte...

Pang-hung Chen, 陳邦鴻

碩士 / 東吳大學 / 微生物學系 / 94 / In crustacean, both prophenoloxidase activating system (PAS) and phagocytosis play important roles in the immune system and are believed to be used as the defense indicator (Hernandez-Lopez et al., 1996; Le Moullac, 1998; Rodriguez & Moullac, 2000). Chuo et al., (2004) found that PAS can be triggered via either G protein/protein kinase C (PKC) or cAMP pathway. In (the research of) mammalian, signal pathway of CpG ODN stimulation starting from the acidification of endosom...

周虹君

碩士 / 國立彰化師範大學 / 生物技術研究所 / 95 /

Hui-Fen Liu, 劉慧芬

碩士 / 國立彰化師範大學 / 生物學系 / 92 / In this study, two membrane form guanylyl cyclases (mGC) were cloned from the abdominal muscle of the crayfish Procambarus clarkii－PcGC-M2 (5,158 bp) and PcGC-M2’ (4,989 bp). PcGC-M2, a typical mGC, contains an open reading frame encoding 1,403 amino acid residues. Sequence analysis indicated that PcGC-M2 is composed of an extracellular domain (ECD), a transmembrane domain (TM), a kinase homology domain (KHD), a guanylyl cyclase catalytic domain (GCD) and a C-terminal...

HU YEN TSUNG, 胡延宗

碩士 / 大葉大學 / 資訊管理學系碩士在職專班 / 93 / Chinese Character Input is the basic and important ability to use Chinese Computer. The efficiency of using Chinese computer depends on the performance of Chinese Character Input. Therefore, it is crucial to choose a better-efficient Chinese Character Input system and master it, so that Chinese computer users can lay a solid foundation in using computers. 　　The purpose of this study was to compare the performance of Dy-Sin-Chang-Jie and Wu-Sia-Mi, both of which we...

黃彥棟

碩士 / 東吳大學 / 微生物學系 / 92 / In crustacean, the prophenoloxidase (proPO) activating system plays an important role in the nonspecific defense system. Our previous studies have proven that, in freshwater prawn Macrobranchium rosenbergii, the PO activity is related to the defense functions and can be enhanced by immunostimulants. The activity is easily changed by many intrinsic and extrinsic factors; therefore, it is not suitable to use the PO activity as a criterion for the activation of proPO system...

Wen-ching Hsu, 徐玟靖

碩士 / 嘉南藥理科技大學 / 化妝品科技研究所 / 99 / In this study, cytotoxic levels of astaxanthin, Glossogyne tenuifolia and Camellia oleifera flowers on 3T3 mouse fibroblasts were measured by MTT assay. Further, we investigated the inhibitory effects on activation of secreted matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in culture medium using gelatin zymography. Collagen protection effect was researched subsequently using collagen assay kits. DPPH radicals scavenging activity, tyrosi...

Chiao-Chuan Han, 韓僑權

博士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 98 / In this study, mtDNA was used to examine the phylogenetic and molecular relationships of the freshwater shrimp family Atyidae. The phylogeographical variations of two landlocked Caridina pseudodenticulata and Neocaridina denticulata were also discussed. Four genera and 32 species of the Atyidae were identified and divided into 6 groups. In addition to 14 previously recorded species, eight of them are new records, while another 10 species remain unconfirmed. Th...

Yi-Houg Chou, 周易宏

碩士 / 大仁科技大學 / 環境管理研究所 / 92 / Culture Penaceus japonicus in shrimp pond by growing plankton and snails without adding any feed, any with food chain. During the period of 30 days to 90 days. The samples include Penaceus japonicus (separate into viscera, shell and muscle), Mugilogobius parvus, Thiara granifera, Chara sp., Entermorpha intestinalis, pond water and sediment were sampled for analysis every 3 days. 180 samples drawn were conducted with ICP-AES element analysis. For analysis were the Ma...

Jun-Hao Su, 蘇俊豪

碩士 / 東吳大學 / 微生物學系 / 98 / Endocrine disrupting chemicals (EDCs) which are xenobiotic chemicals such as phthalate esters (PAEs) can interfere with the endocrine functions of orginasms. In this study, the expressed sequence tag (ESTs) system developed from shrimp Neocaridina denticulate and four PAEs of aquatic pollutants, including diethyl phthalate (DEP), dihexyl phthalate (DHP), dipropyl phthalate (DPrP) and diphenyl phthalate (DPP), were used to study the effect of PAEs on physiological functio...

Ya-Ting Nien, 粘雅婷

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 98

Hui-Ju Chang, 張惠茹

碩士 / 國立嘉義大學 / 食品科學系研究所 / 98 / In this study, persimmon leaves and persimmon are raw materials and the extracts of them are used to be a natural preservative to prolong the shelf life of shrimps. Further persimmon leaves and persimmon extracts are divided into different fractions by XAD column and determine their antibacterial ability and composition. Persimmon leaves and persimmon get from bull heart persimmon (Diospyros kaki L. cv. Bull heart) as raw materials. The extracts of persimmon leaves ...

Chun-Ping Chu, 曲君平

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 100 / White spot syndrome virus (WSSV; genus Whispovirus, family Nimaviridae) is a widely occurring virus which attacks cultured shrimp and many other crustaceans and has caused severe mortalities and huge economic losses for the global shrimp farming industry. Currently, no effective method is available for treating this disease caused by WSSV infection. However, recent studies have reported host cell proteins related to WSSV infection. This study, cloned full length o...

pay-jung huang, 黃姵蓉

碩士 / 佛光人文社會學院 / 教育資訊學研究所 / 94 / The purpose of this study is to find out the influences of two factors on learning the Boshiamy Chinese key-in system. The factors include the practice methods and the basic English ability of the participants. The practice methods include the Root method and Character-splitting method. Since the root of the Boshiamy Chinese key-in system is based on the figures, phonetic sounds or meaning corresponding to 26 English letters, the second purpose of this research...

Lee, Fang-Yi, 李芳儀

博士 / 國立海洋大學 / 水產養殖學系 / 85 / Female-specific protein (vitellogenin) was purified from mature female hemolymph of the freshwater prawn, Macrobrachium rosenbergii by DEAE, hydroxylapatite and another DEAE chromatographic columns. A purified vitellogenin with an apparent molecular weight of 700 kDa was obtained on the basis of by PAGE. The purified vitellogenin was considered as a lipo- glyco-protein on the bas...

Ya-ChuChuang, 莊雅竹

碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 99

Yi-Ming Tai, 戴宜銘

碩士 / 中興大學 / 食品暨應用生物科技學系 / 95 / Astaxanthin, one kind of carotenoids, has been used as a pigment source in the poultry and aquaculture industries to improve the red color of products. Due to the color and antioxidative activity, astaxanthin can also be used as food colorant and antioxidant in medicine. In Japan, astaxanthin had been produced as anticancer agents. Fructooligosaccharides (FOS) are widely found in natural plants and are also synthesized from sucrose by transfructosylation of microbi...

Yuan-Di Tzeng, 曾元帝

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 95 /

Chien-Pang Chung, 鍾建邦

碩士 / 東吳大學 / 微生物學系 / 98 / A specific primer was designed to perform 3’- and 5’-RACE PCR (rapid amplification of cDNA ends by PCR), which was used to clone the full length (780 bp) of cDNA suspected to be an anti-lipopolysaccharide factor (ALF). The polyadenylation signal ranged from nucleotides 752 to 762. For translation of the fragment, the 5’UTR consisted of nucleotides 1 to 482 and the 3’ UTR contained nucleotides 483 to 780. The open reading frame (ORF) included nucleotides 102 to 482, yield...

CHEN, XIU-JIN, 陳秀錦

碩士 / 國立海洋大學 / 水產養殖研究所 / 80 /

LIAO, XIAN-HONG, 廖先鴻

碩士 / 國立海洋大學 / 水產養殖研究所 / 80 /

Hsin Hua Lee, 李欣樺

碩士 / 東吳大學 / 微生物學系 / 90 / In insect and crustacean, propheoloxidase (proPO) activating system plays an important role of the defense and recognition functions. The previous studies have proven that the PO activity is related to the defense function of the giant freshwater prawn Macrobranchium rosenbergii. However the enzyme activity was easily influenced by the environmental conditions. Therefore, the PO activity is not able to be used as a parameter for activation of proPO system. In ...

LIU, BING-ZHONG, 劉秉忠

碩士 / 國立海洋大學 / 漁業研究所 / 73 /

Huei-Mei Shih, 施惠美

碩士 / 國立清華大學 / 生命科學系 / 90 / Short-term potentiation (STP) and long-term potentiation (LTP) are two forms of synaptic facilitation. Much of the experimental work concerns the possible roles of STP and LTP in memory formation. Both STP and LTP can be induced by high-frequency stimulation in hippocampal area CA1 that are the phases of population excitatory postsynaptic potential. Many studies of conditioned that they are the activity-dependent increase in synaptic efficacy and through chemical synap...

Hsing-Wen Hung, 洪幸彣

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Astaxanthin (AST), a non-pro-vitamin A carotenoid, is abundant in aquatic animals such as crab, shrimp etc. It possesses some pharmacological activities, including anti-inflammatory, anti-oxidative and immunomodulatory properties. IgE-mediated allergy owing to hyper-reactive immune responses to antigen in the environment. It shows high relationship beteewn reactive oxygen species (ROS) and allergic response through modulating intracellular signal transduction pathway...

Yu-Ting Huang, 黃鈺婷

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Abstract Recently, oxidative stress has been implicated in the progression of Alzheimer’s disease and antioxidants may protect neural cells from this attack. Astaxanthin (AST) is a carotenoid found in marine animals and alga. Several previous studies have demonstrated that AST exhibits a wide variety of biological activities including antioxidant, antitumor effects. In this study prove 50 □M AST reduce DNA fragmentation caused by H2O2 through inhibition of about 50% ...