He-Shun Wang, 王河順

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 94 / Abstract Grey mullet (Mugil cephalus) exposed to different salinity (0, 17 and 34 psu) and lead concentrations. The fishes exposed at 17 psu are the most tolerant to lead. The 96-h LC50s were 1.65, 6.25 and 4.39 Pb2+ mg/l following exposed to 0, 17 and 34 psu. Examined the LT10, LT50 and LT100 lethal time is also the same trend with concentrations by 17 psu > 34 psu > 0 psu. Oxygen consumption (O2 mg/g/h) and ammonia excretion (N mg/g/h) of M. cephalus were mea...

Tsui-Yao Chen, 陳翠瑤

博士 / 國立臺灣海洋大學 / 食品科學系 / 94 / Abstract I-Tiao-Gung (Glycine tomentella Hayata) has been wildly cultured in Kinmen and used as a folk medicine for treatment of rheumatism, gout, arthritis, and ache of physique indicative of its anti-inflammatory activities. But its biological activities and pharmacological functions have not been studied systematically. The purpose of this study was to elucidate the long-claimed anti-inflammatory activity of I-Tiao-Gung by in vitro test and to assess its potentia...

Cheng-Jang Jiang, 江承璋

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / Abstract Many academics used zebrafish to study，embryo development and morphogenesis of zebrafish is similar to mammalian，therefore we selected zebrafish for study purpose。Cancer is human major crisis and Hepatocellular carcinoma is also the most magliant cancer in Taiwan area。Establish animal model on zebrafish，By making use of this model to study cancer questions and we select tet-on system to regulate gene activation。We used liver fatty binding protein (L-FABP...

Peng-Chi Chen, 陳鵬及

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / “Chinese herbs nephropathy” (CHN) is a rapidly progressive nephropathy that was first described in 1992. Vanherweghem and his colleagues in Belgian found that women who had followed a particular weight-reducing regimen that included Chinese herbs developed CHN. Further plant biochemistry analyses revealed that the weight-reducing regimen contained the components of aristolochic acid (AA), which has been identified to be one of the causes to CHN. Robisch further sho...

Bo-Hung Lin, 林柏宏

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / The genetic mechanisms of vascular development during embryogenesis and post embryogenesis are largely unknown. We use transgenic zebrafish that express EGFP in blood vessel endothelial cells to study vascular development. Utilizing ENU mutagenesis, we isolated one mutation, called prp （for persistent plexus）that displayed defects in regulating vascular plexus formation in the fins. In prp mutation, vessel plexuses appear constantly in the tips of each fin ray whic...

Shih-Hsiang Lin, 林士翔

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / The zebrafish has emerged as an excellent model organism for study. External development and optical clarity during embryogenesis allow for visual analyses of early developmental processes, and high fecundity and short generation times facilitate genetic analyses. The gene function way is similar to mammal and the organ forms of gene control and embryo's development is similar to other vertebrates. Tetracycline-controlled system (Tet-on and Tet-off) that could be o...

Feng-Ju Hsieh, 謝豐如

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / ABSTRACT Dipyrimidine photoproducts induced by UV irradiation on DNA are primarily removed from UV-damaged DNA by nucleotide excision repair (NER). NER proteins homologous to the human XPA were not detected in the extracts of zebrafish (Danio rerio) embryos, yet these extracts were previously found to contain two 30 to 35 kDa UV-damaged-DNA binding proteins homologous to the 150 kDa vitellogenin1 (Vg1). The objectives of this research were to examine if multiple Vg...

Cheng-Hung Lai, 賴政宏

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / Innate immune system of fish includes antimicrobial and antivirus genes. MX gene is interferon inducible gene. And it’s functions mainly through preventing dsRNA virus from replicating. Antimicrobial peptide (AMP) that can be found among different kinds of organism, plays important roles on first line of defense of pathogen infection. AMP is a physiologic barriers of innate immune. recently, grouper larva was seriously infected by virus and bacteria, hence manipulat...

Tse-Wen Pao, 鮑澤文

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / In the recent year, many iridoviral-like infections has been reported. But the classification of these iridovirus were not clear enough, so this thesis continued the previous research for classification of fish iridovirus. From 1995 to 2004, we collected the iridovirus-like isolates from agriculture grouper, perch, giant grouper, cobia and some other iridovirus-like isolates that collected after that collected afterward. We compared the cytopathological presentation...

Shao-Kun Lee, 李少琨

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / Mitf (Microphthalmia-associated Transcription Factor) is a member of the basic helix-loop-helix leucine zipper (bHLH-Zip) protein family. During the embryonic development, it plays an important role to contol the differentiation from the neural crest cell to melanocytes, mast cells and retinal pigment epithelium cells. It’s also control the survival of melanocytes and regulate the expression of tyrosinase, tyrosinase related protein. PTPS (6-pyuvoyl-tetrahydropterin...

Chien-Wei Tu, 杜健瑋

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / β-glucan and sodium alginate at different dietary doses (0, 0.4, 0.8, 1.6, 3.2 and 6.4 g/kg diets) were administered individually to know the effect on growth, survival rate, non-specific immune parameters and physiology parameters of grouper (Epinephelus coioides) in a long term period (98 days). A same experimental design was administered to know the effect on non-specific immune parameters in a short term period (30 days). The result in experiment I showed that u...

Yu-Ting Zhang, 張鈺廷

碩士 / 國立臺灣海洋大學 / 海洋資源管理研究所 / 94 /

Hoyeh Chang-Thing, 何葉常青

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 93 / Abstract Mugil cephalus exposed to 0(control),1, 5, 10 and 20 ppm Pb2+ for 1,5,10 and 20 days at different salinity (0, 17 and 34 psu) were examined blood oxyhemoglobin, deohemoglobin, methmoglobin, pH, pO2, pCO2, glucose, lactate and GOT(glutamic-oxalacetic transminase). Blood deoxyhemoglobin decreased directly with ambient Pb2+ concentration and exposure time, where as methemoglobin was inversely related to ambient Pb2+ concentration and exposure time. Mugil ...

Hsin–Yu Tseng, 曾心榆

碩士 / 國立高雄師範大學 / 生物科學研究所 / 93 / Abstract The main purpose of this study is to develop in vitro propagation of Pluchea indica (L.) Less, belonging to the family Asteraceae, and examine its response on salinity and drought stress. In vitro culture of Pluchea indica initiated from mature seeds or aseptic cutting plantlets on 1/4MS medium without plant growth regulators showed the best growth and rooting ability. Growth responses of plants cultured on 1/4MS supplemented with different concentration o...

Hsin-Hsiung Lee, 李新雄

碩士 / 華梵大學 / 資訊管理學系碩士班 / 94 / The Internet becomes more popular to promote the higher rapidly development of the new online business model, E-commerce. The E-commerce has brought the more benefits and convenience on the personal consumption, the regional economy, the global logistics as well as the online e-services. However, the online theft and attacks through the Internet tend to be on the rise progressively to gain illegal blood money. The Phishing attack is one of the major fraud crimes, whi...

Lee Jheng-Che, 李政徹

碩士 / 長庚大學 / 復健科學研究所 / 94 / Postural stability during quiet stance has been used as an index to evaluate balance performance in patients with neurological disease and elderly. Especially, postural stability during quiet stance, the degrees of postural sway, have been found to correlated with falls incidence in elderly. Previous studies found that enhanced cutaneous inputs from the feet improve postural stability in young adults. However, the effect has not been confirmed in elderly population. Fu...

Ho, Hsin-Yi, 何心怡

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 94 / Cobia (Rachycentron canadum) is one of the most important cultured fish in Taiwan providing for Sashimi use. Their skin wastes produced from fillet processing contain a great amount of collagen which can be utilized to make high value-added products. Many researches reported that hydrolyzed peptides and proteins have functional properties and can be used as supplement or health food. For the reasons, the aim of this study is to investigate the molecular weight ...

Yen-Hung Yeh, 葉彥宏

博士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Some Chinese people believe that eating the animal gallbladder, such as bile of grass carp (Ctenopharyngodon idellus), snake and chicken, may improve their visual acuity. However, poisoning cases have occasionally occurred when people ingested animal bile, especially grass carp bile. Among those cases, the major symptom of victims was acute renal failure. Disturbance of liver, gastrointestinal tract and central nervous system were also noted. The major toxic material...

Chun-in Lin, 林俊印

碩士 / 國立中山大學 / 海洋生物研究所 / 94 / Two feeding trials were conducted to evaluate the effects of dietary phytic acid contents and removal of phytate from plant protein sources on growth and utilization of phosphorus, zinc and iron in juvenile cobia. In experimental I, test diets were formulated by adding phytic acid, 0, 5, 10, 15 and 20 g/kg diet to the basal diet that used fish meal and wheat gluten as the protein source. Juvenile cobia with an initial weight of 20g were fed the test diets for 8 weeks...

Ya-wen Hsu, 許雅雯

碩士 / 國立陽明大學 / 生理學研究所 / 93 / Muscarinic acetylcholine receptors (mAChRs) are G-protein-coupled receptors with seven putative transmembrane domains. They play important roles in neuronal and non-neuronal functions, such as cell proliferation, differentiation, apoptosis, etc. In the past decades, zebrafish has become a model organism to study vertebrate development. We use the zebrafish as model to study the possible role of M2 mAChR (M2R) in blood and blood vessel formation in embryos. Previously,...

Yu-Hui Li, 李郁蕙

碩士 / 國立中興大學 / 生命科學系所 / 94 / Cadmium(Cd) is a non-essential metal for the organisms, widely used in industrialized countries. The Itai-itai disease caused by cadmium contamination has made a serious impact on human health for its bioaccumulation through the food chain. Cadmium represents a heavy metal pollutant in Taiwan. Under the Cd2+ exposure, fresh water fish experiences growth inhibition and even death. Homeostatic mechanisms are required for all organisms to minimize the toxicity of cadmium...

Shao-Yang Hu, 胡紹揚

博士 / 國立臺灣大學 / 微生物與生化學研究所 / 92 / The mature tilapia insulin-like growth-2 (IGF-2) cDNA was cloned into pET28b(+) vector and expressed in Escherichia coli. In flask culture, the transformant E. coli BL21(DE3) harboring pET-IGF2 expressed the recombinant IGF-2 as inclusion bodies, and the content was as high as 10.3% of the total protein content after 9 hr 0.06 mM IPTG induction. For production of recombinant IGF-2 in E. coli, pH-stat fed-batch cultures were used to achieve a high cell density cult...

Geng-ChengTsai, 蔡庚呈

碩士 / 國立成功大學 / 生物科技研究所 / 104 / Tumor necrosis factors-α (TNF-α) is a multi-function cytokine, which plays an important role in innate immunity. Previous research indicated that orange-spotted grouper (Epinephelus coioides) has two types of TNF-α, TNF-α1 and TNF-α2. The stimulation of recombinant TNF-α1 and TNF-α2 has shown the cross activation in each other. In this study, the recombinant TNF-α form homotrimer when interacts with cross-link reagent. In double immunofluorescence staining shows col...

Tsfng-Ming Lu, 盧增明

碩士 / 國立中興大學 / 水土保持學系所 / 102 / Rural rejuvenation fund was set up by the central competent authority. A bottom-up governance structure concept is worth identity. However, the effectiveness and impact of regulations arising rural rejuvenation is quite novel issues. Various aspects of the field are to be more in-depth research. Therefore, five indicators, namely, improve the overall environment of rural communities and public facilities, industrial activation, cultural preservation and utilization...

Hsu-Chin Kuo, 郭旭芩

碩士 / 國立屏東科技大學 / 環境工程與科學系所 / 102 / The distributions of endocrine disruptors were conducted in the riverine environment in many reports. However, little is known about the induction of vitellogenin in male fish by estrogenic chemicals in Taiwan’s river. This study aimed to determine the concentrations of endocrine disrupting chemicals: estrone(E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), diethylstilbestrol (DES), nonylphenol (NP), octylphenol (OP) and bisphenol-A (BPA) in up...

Huai-Ren Fan, 范懷仁

碩士 / 國立屏東科技大學 / 景觀暨遊憩管理研究所 / 102 / B&;B Industry have become a new tourism trend in the ever-changing, innovation-seeking modern society of Taiwan. The fierce and rapid competition has resulted in changes in the B&;B Industry sector as professional B&;B Industry operation replaces the former mode where B&;B Industry merely provide accommodation. Many B&;B Industry operators have come to the understanding that their B&;B Industry may be unlikely to attract guests if the B&;B Industry operation re...

WEN-LING LIN, 林玟伶

碩士 / 國立屏東科技大學 / 動物疫苗科技研究所 / 101 / Viral infections have been increasingly reported in cultured marine fish. Nervous necrosis virus (NNV) is one of the most devastating pathogens, infecting a variety of marine fish all over the world and bringing about high mortality in the larval grouper. Viral nervous necrosis (VNN) or viral encephalopathy and retinopathy (VER) is one of the most important viral diseases in aquaculture, and has caused mass mortality in more than 34 species of fish at larval and...

Jheng-Yao Huang, 黃政堯

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 101 / The objective of this study is to explore the energy metablism of tilapia (Oreochromis mossambicus) upon acute salinity exposure. This study is divided into four groups of treatment include: first through brackish water (20 ‰) adapt one or two days and then transferred to 28 ‰ seawater (respectively to BW1d-SW; BW2d-SW representation); transferred directly to 20 ‰ brackish water group (FW-BW); transferred directly to the group of 28 ‰ seawater seawater (FW-SW). A...

Liu Jia-Hao, 劉家豪

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 101 / Metallothionein (MT) has been found over 50 years, There were many isoforms of MT in zebrafish, but most studies always focused on the MT2. The similar metallothionein B (sMT-B) was found in 2005. However, it was not clear about its roles on fish. Thus, the aim of the present study was to compare the physiological role of the both isoforms of zebrafish MT. The present study will detect the mRNA expression of MT-2 and sMT-B with a real time PCR upon cold stress (a...

Jia-Jen Chen, 陳佳珍

碩士 / 國立臺灣大學 / 漁業科學研究所 / 89 / The utility of vegetable protein sources in the diet for freshwater fish has been studied by many researchers. These studies were conducted to determine the value of heated (120 ℃, 40 min ) lupin seed meal (HL) as replacement for fish meal (FM), soybean meal (SM) and rice bran (RB) in the diet of tilapia (Oreochromis mossambicus). On the other hand, insulin-like growth factor (IGF) was incorporated in the diet containing 25 % HL and un-treatment lupin seed meal (UL) ...

BorJyh Guan, 官柏志

碩士 / 國立臺灣大學 / 漁業科學研究所 / 89 / Abstract Infectious Pancreatic Necrosis Virus (IPNV) belonging to the Birnaviridae with bisegmented double-strand RNA genomes is one of the widespread fish pathogen and could infect many economically important finfish and shellfish. According to previous study in our laboratory, IPNV infection could induce apoptosis in chinook salmon embryo cell line CHSE-214. To clarify the regulation mechanism in IPNV-induced apoptosis, this thesis is attempted to i...

Yu-Tuan Tsai, 蔡育端

碩士 / 國立中山大學 / 化學系研究所 / 91 / none

Chia-ling Su, 蘇佳鈴

碩士 / 南華大學 / 旅遊事業管理學研究所 / 91 / This study aims to explore the relationship between image, satisfaction and loyalty for the tourists who attending the Ping-Tung Toro Cultural Festival in 2002. Some multivariate analysis techniques including descriptive statistics, factor analysis, and one-way ANOVA are used in this study to analyze the socio-demographic profile of tourists. LISREL is applied to establish the relationship model and investigate the relationship among the latent variables under study...

Zhen-Rui Wang, 王振叡

碩士 / 國立臺灣大學 / 地理環境資源學研究所 / 91 / Many in situ experiments have detected high concentrations of xenoestrogen in the river downstream from high magnitude of human activities. The objective of this study is to understand the risk for fish exposed to this kind of “stress”. First, a “dose and response” experiment was carried out for nonylphenol in male guppy (Poecilia reticulata). Second, Taoyuan County was selected as a study region, because there is high density of population and textile industry de...

Yi-Fan Chen, 陳逸帆

碩士 / 國立高雄大學 / 生物科技研究所 / 95 / PTEN is a tumor suppressor, it also regulates cell size and cell cycle. Organogenesis requires the increases of cell size and cell number as well as the support of vascular system. VEGF, a selective mitogen for vascular endothelial cells, is important for the development of vascular system. But it is not clear if PTEN controls tissue mass by suppressing angiogenesis provoked by VEGF and their correlations on the postnatal tissue development in fish. We used a primiti...

Chen Chia Huang, 陳加晃

碩士 / 國立中興大學 / 生命科學院碩士在職專班 / 92 /

Hu, Fang-Yu, 胡芳瑜

碩士 / 國立交通大學 / 環境工程系所 / 103 / Semiconductor manufacturing industry is flourishing in Taiwan, which effluent is harmful to environment due to the existence of various substances, such as NH4+, TMAH and Cu(II). Although the amount of the individual substance in the effluent is low, the combined effects on aquatic organisms should be concerned. The aim of this study is to examine individual and combined toxicity of NH4+, TMAH and Cu(II) on Daphnia magna and zebrafish (Danio renio) embryos in the syn...

Chiu-li Kao, 高久理

博士 / 國立中山大學 / 生物科學系研究所 / 103 / Nasopharyngeal carcinoma (NPC) is a head and neck cancer that rarely occurs in most parts of the world. In contrast, the highest incidence was found in the southern Chinese population of Guangdong, Inuits of Alaska, and native Greenlanders. Pluchea indica (L.) Less is a well-known indigenous medicinal plants that grows in littoral areas of tropical regions of countries such as Malaysia, Indonesia, Australia, Taiwan, India, and Mexico. The plants have been tradition...

Hsin-Yi CHOU, 周心怡

碩士 / 國立中興大學 / 獸醫病理學研究所 / 89 / Abstract Fish immunity is influenced significantly by the fluctuation of environmental temperature due to fish belong to the poikilothermic animals. It is generally accepted that higher temperatures would enhance the specific immune responses whereas lower temperatures adversely affect their expression. However, few data are available to explain the temperature effects on nonspecific defense mechanism in fish. The aim of this study is to evaluate the ef...

Liu,Tang-Bi, 劉棠必

碩士 / 國立臺灣大學 / 動物學系 / 84 / A phosphotyrosyl protein phosphatase (PTPase) was purified from the hepatic nuclear matrix of Tilapia mossambica with a final specific activity of 5,095 units/mg of protein. 2 % CHAPS and 2M NaCl were applied to extract the matrix bound protein and one affinity column,two anion exchangers plus one gel filtration column were conducted consecutuvely to purify the enzyme. The PTPase h...

Chen, Jeng-Ping, 陳正平

博士 / 國立臺灣大學 / 海洋學系 / 84 / Gymnapogon sp.1 and G. sp.2, one new record Apogon albomarginatus, and one questioned species A. bandanensis are newly added. Totally, there are 2 subfamilies, 13 genera and 77 species of Apogonidae recorded in Taiwan. In the present paper, the references of apogonids in Taiwan are revised, a diagnostic key and four photos of newly added species are provided. Thus, the...

Shen, Bi Zheng, 沈必正

碩士 / 國立海洋大學 / 水產養殖研究所 / 84 /

Chen, Kang-Ching, 陳康青

碩士 / 國立海洋大學 / 海洋生物研究所 / 84 /

Wang, Ying-Wei, 王英偉

碩士 / 國立海洋大學 / 水產養殖學系 / 84 /

Shieh, June-Shyang, 謝俊祥

碩士 / 國立海洋大學 / 水產養殖學系 / 84 /

Zhi-Hao Lee, 李志浩

碩士 / 國立澎湖科技大學 / 水產資源與養殖研究所 / 102 / This study explores the effect of dietary protein and lipid on the growth performance, body composition, liver and effects on hepatic lipid peroxidation of juvenile rock porgy (Oplegnathus fasciatus). In the first experiment, all five diets were estimated to be isoenergetic diets containing different dietary protein levels (35%;40%;45%;50% and 55%). Fish meal and cod liver oil were the main dietary protein and lipid source. The initial weight of fish is 8.0...

Chen, Jyh-Yih, 陳志毅

博士 / 國立臺灣大學 / 動物學研究所 / 85 /

Chen, Chih-Yu, 陳治宇

博士 / 國立臺灣大學 / 動物學系 / 86 / Blooming of Alexandrium minutum Halim in aquaculture ponds are always followed by the mass mortality of fish or accumulation of PSP toxins in the purple clam (Hiatula rostrata Lighttoot). This research focused on the application of HPLC on PSP toxin composition in A. minutum, in purple clam that fed on the dinoflagellate, in ivory shell ...

張世倉

碩士 / 國立海洋大學 / 漁業科學研究所 / 86 / Wushykeng Creek is a 12-kilometer long mountain stream, a tributary to the Daan River, situated in the Taichung county in the central portion of Taiwan. In 1987-1989 the Taiwan Forestry Bureau constructed seven check-dams (sabo-dams) for the stream bed stabi ization, with nine fishways. From July 1995 to May 1997, the stream survey was conducted at eight stations to collect data on physical environment (stream flow, stream width, water depth, water velocity, bottom s...

彭翠儀

碩士 / 國立海洋大學 / 水產養殖學系 / 86 /