Kuan-Ming Huang, 黃冠銘

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 99 / Cadmium (Cd) is a carcinogenic heavy metal which may exert its carcinogenicity by decreasing the efficiency of DNA repair. Our previous studies indicated the ability of Cd ion to down-regulate the gene expressions of MutS homolog 2 (MSH2) and MSH6 that initiates DNA mismatch repair (MMR) of simple mispairs and small insertion-deletion loops in vertebrates using zebrafish (Danio rerio) embryo as a model organism. This study explored the mechanisms of Cd-induced inhi...

Yi-Pei Lin, 林宜霈

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 99 / The purpose of this thesis is study what Progranulin (Pgrn) by GH. Pgrn is a growth modulating factor released by a variety of cells because of the potential functions displayed in the regulation of development, wound healing and progression of several cancer types. In our previous study, we found that hepatic Pgrn and IGF-1 were co-induced with human growth hormone (hGH). Although it is well known that activation of IGF-1 by growth hormone is via JAK-STAT5b signal...

Hsi-Wen Pang, 龐皙文

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 99 / In recent years, non-alcoholic fatty liver disease (NAFLD) has become the highest prevalence of chronic liver disease, and its also been highly correlated with some other human chronic disease, e.g. obesity, diabetes or hypertension. High-fat diet and alcohol intake will produce fatty acid accumulation in liver, the main reason for fatty acid abnormal accumulate in the liver might be hepatic lipid metabolism dysfunction. Carbohydrate response element-binding protei...

歐庭瑜

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / Spermidine/spermine-N1- acetyltransferase 1 (SSAT1), a polyamine catabolic enzyme in animals, was found to take parts in not only polyamine homeostasis but also many other physiological events, such as lipid metabolism and hypoxia response. To understand the regulatory mechanisms and the enzyme properties of SSAT1 would help to elucidate its diverse physiological functions. First, we try to view this issue from an evolutionary perspective. Using cross species gen...

Chia-Hsiang Hsu, 許家祥

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / This investigation elucidates the interaction of dietary vitamin C (L-ascorbyl-2-monophosphate-Na, C2MP-Na) with inorganic copper (Cu, CuSO4) and organic Cu (Cu-peptide) on the growth and immune responses of grouper, Epinephelus malabaricus. The amount vitamin C and Cu added to the basal diet in representing the adequate dietary level for grouper were derived from previous results. Basal diet containing either adequate or 2X adequate organic and inorganic Cu were eac...

Yi-Hua Wang, 王怡驊

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / The effects of supplemental crystalline amino acids in feed on ammonia excretion、blood parameters and plasma amino acids in Tilapia ( Oreochromis niloticus × O. aureus). All diets were formulated as the crude protein content of 30%, crude lipid content as 6%, divided into fish meal protein group (Control), vegetable protein source group (Basal) and plant protein sources added crystalline lysine group (Lys, Lys 4 ×, Lys 8 ×). In the first experiment, the Tilapia feed...

Ping-Shen Shih, 施並伸

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / The objective of the present study was to investigate the effect of bath treatment of different concentrations (0.5, 1.5, 3 mg/L) of benzalkonium chloride (BKC) on its residue depletion in red tilapia (Oreochromis spp.), water and soil. In red tilapia, fish following bath treatment with different does of BKC had water replacement after 24 houres. Sample of gill, serum, liver, skin, muscle were collected at 2, 4, 8, 16, 24, 48, 120, 192, 360, 720, 1440 hours post dos...

Che-Wei Hu, 胡哲瑋

碩士 / 國立臺灣大學 / 漁業科學研究所 / 99 /

Yu-Ling Yen, 顏郁玲

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / This investigation elucidates the interaction of dietary vitamin C (L-ascorbyl-2-monophosphate-Na, C2MP-Na) with inorganic Manganese (Mn, MnSO4) and organic Mn (Mn-peptide) on the growth and immune responses of grouper, Epinephelus malabaricus. The amount vitamin C and Mn added to the basal diet in representing the adequate dietary level for grouper were derived from previous results. Basal diet containing either low (1/2 adequate) or adequate organic and inorganic M...

Chian-Chi Huang, 黃千綺

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / IGF2 mRNA-binding protein 2 (IGF2BP2/IMP2) was expressed in fetal liver but little in adult liver while overexpressed in the human liver tumors including hepatocellular carcinoma and cholangiocarcinoma of human. To study the molecular mechanism of IMP2 during the progression of liver tumors, transgenic zebrafish lines Tg(fabp10:tetoff; tre:imp2/gfp) with constitutively zebrafish IMP2 and GFP-overexpressed in the liver had been established in our laboratory by using ...

Wen-Yen Chang, 張文彥

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 99 / MicroRNAs (miRNAs) are short, endogenous non-coding RNAs that regulate gene expression at the post-transcriptional level by targeting the 3’-untranslated region (3’UTR) of mRNAs through a conserved seed sequence. MiR-206 is a muscle-specific miRNA and is highly expressed in skeletal muscle. Although miR-206 plays a key regulatory role in myoblast proliferation and differentiation, the detailed molecular regulatory mechanism of miR-206 in early myogenesis is still...

Ying-Fang Su, 蘇盈方

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 99 / Myf5, one of the myogenic regulatory factors, plays important roles in the specification and differentiation of muscular cells during myogenesis. In zebrafish, an intronic microRNA (miR), miR-3906, located within myf5 intron I, has been reported to silence the translation of its target gene, dickkopf-3-related (dkk3r) gene. Dkk3r, a secretory protein, regulates the phosporylation of p38a to maintain Smad4 stability, which, in turn, enabling the Smad2/Smad3a/Smad4...

Chia-Huei Chen, 陳佳慧

碩士 / 國立臺灣大學 / 漁業科學研究所 / 99 / Grouper aquaculture has been threaten by grouper iridovirus (GIV) and nervous necrosis virus (NNV) for many years, and the insufficient apply of long chain unsaturated fatty acids for grouper metamorphosis development and neural growth, all result in a high mortality rate for grouper fry. Therefore, to culture a high quality grouper fry of specific pathogen free (SPF) becomes an important issue for nowadays grouper aquaculture. Rotifer and artemia nauplius, which are...

Chun-Hung Lin, 林均鴻

碩士 / 國立臺灣大學 / 農業化學研究所 / 99 / Triadimefon and myclobutanil are triazole-containing conazole fungicides widely used in agriculture. Previous studies show that some conazoles (e.g. triadimefon) at sublethal dose induced gene expression and enzymatic activity of cytochrome P450s (CYPs) in the liver of mouse, leading to a decrease in hepatic level of all-trans retinoic acid (atRA), an active form of vitamin A with anticancer properties. This study demonstrates the effects of carcinogenic triadimefon ...

Chia-Yu Chang, 張嘉育

碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / This study focused on the different kinds and concentrations of carotenoid in diet, for the effects of pigmentation of pearlscale goldfish (Carassius auratus), assessment of pigment metabolism, and raise fish pigmentation in the suitable amount. Experimental feeds are supplemented with two different carotenoid: zeaxanthin (hereinafter referred to as Z), synthesis astaxanthin (hereinafter referred as S). Experiment period was total 7 weeks, mean weight of pearlscale ...

Cheng-Hsien Hsu, 許政憲

碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / Grouper is one of warm water coral reef fishes and can survive in a broad range of salinity. It was the popular cultured fish and grows rapidly and has delicious meat. It was also the economic fish well received by consumers of Southeast Asia. The experiment was conducted to study the effects of vegetative ingredients, lipid and n-3 highly unsaturated fatty levels replacing fish meal on growth performance of pearl grouper (Epinephelus fuscoguttatus × E. lanceolatus)...

Han-Wei Wang, 王翰偉

碩士 / 國立臺灣大學 / 臨床牙醫學研究所 / 101 / Bisphosphonates have been predominately applied in the treatment of osteolytic bone diseases, such as osteoporosis, Paget''s disease, multiple myeloma, and cancer-related metastatic bone lesions for the past several decades. Their clinical applications have alleviated the osteolytic symptoms, pain and fractures entailed by those diseases. However, the implications of those drugs have led to new impacts on clinical dental treatments. Recently, an increasi...

Yi-Ruei Chang, 張宜叡

碩士 / 國立臺灣大學 / 農業化學研究所 / 101 / Polyamines (PAs) are organic compound with two of more primary amino groups (such as putrescine, spermidine and spermine), which play vital roles of cells and widely found in the all kinds of species. Spermidine/spermine N1-acetyltransferase (Ssat) is a key enzyme, which maintains PA homeostasis. However, there exist three zSsat1s (1a, 1b, 1c) and two zSsat2s (2a, 2b) in zebrafish, which possess different specificities to the PAs. In order to understand the substrat...

Chin-JungChang, 張晉榮

碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 99 / High diversity and multiple serotypes in bacteria are the main bottleneck of cross-protective vaccine development. In previous studies, our lab isolated three protective immunogens, HSP60, enolase and GAPDH in Photobacterium damselae subsp. Piscicida (Ph. d. p.) by immunoproteomics approach. In other studies, Vibrio campbellii was identified as a pathogen in fish farm in Tainan. These three immunogens were also isolated from this V. campbellii by the same approac...

Wei-Lun Lee, 李偉綸

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 99 / Glucose 6-phosphate isomerase (GPI), alternatively named phosphoglucose isomerase (PGI), autocrine motility factor (AMF) or neuroleukin (NLK), is a sugar metabolic enzyme catalyzing the interconversion between glucose-6-phosphate and fructose-6-phosphate. When secreted out of the cell, it can induce cellular activities of neighboring cells. In zebrafish, gpi was duplicated into gpia and gpib. Compared to gpia, gpib was expressed at earlier stage. Using in situ ...

Hsi-Ling Chang, 張喜寧

碩士 / 靜宜大學 / 生態學系 / 99 / Formosan landlocked salmon (Oncorhynchus formosanus) is an endangered species in Taiwan. Though its conservation has lasted for over twenty years by the government, it is hard to say the survival crisis is solved. Shei-Pa National Park is the current state authority for restoring the salmon. It gradually switches the conservation strategy from the state-controlled, elite-centered, and exclusive management to allowing more local-participation and collaboration with the loc...

Chen,Shihlun, 陳示崙

碩士 / 國立高雄海洋科技大學 / 漁業生產與管理研究所 / 99 / The spotted scat （Scatophagus argus） is an euryhaline fish, which is widely distributed throughout the Indo-Pacific Ocean. It is the one of important commercial species in Taiwan fisheries. In this study, intraspecific variation was examined by using mitochondrial DNA cytochrome b gene in 92 individuals sampled from nine localities in the waters off western Taiwan and southeastern China. A total of 1141 bps 64 polymorphic sites, revealing a moderately high hap...

Dao-Ming Lo, 羅道明

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 98 / Due to the transparent zebrafish embryo makes the dynamic observation possible, and also makes the facilitation to study the influence of drug by soaking. We study the effect of Amiodarone, a type III anti-arrhythmia drug, on heart development. After treating embryos with 15 μM Amiodarone from 10 to 72 hours post-fertilization (hpf), a blood regurgitation between ventricle and atrium was observation owing to the defect of valves. Using whole-mount in situ hybridi...

Yi-Chun Chen, 陳儀君

碩士 / 臺灣大學 / 漁業科學研究所 / 103 / Among the breast cancer subtypes, triple negative breast cancer (TNBC) (ER/PR/HER2) is particularly challenging and difficult-to-treat. Combinations of surgery, hormone, irradiation, and targeted therapies are not usually effective to TNBC patients but chemotherapy was shown good therapeutic efficacy. However, failure in TNBCs treatment by chemotherapy is the cause of multidrug-resistant (MDR) cancer cell developed and healthy cells are disrupted, causing adverse s...

Yi-Jing Syue, 薛宜靜

碩士 / 國立嘉義大學 / 獸醫學系研究所 / 99 / In this study, grouper with enrofloxacin 20 mg / kg oral once added to the feed. Using HPLC, detected concentrations of enrofloxacin and ciprofloxacin distribution, in grouper liver, kidney, muscle and serum. The maximal detective concentration in grouper’s liver was 1.36μg/mL at the 8th hours post dosing enrofloxacin of 20mg/kg. After 12 hours post dosing, the maximal detective concentration in kidney and muscle was 15.49 μg/mL and 2.04 μg/mL, respectively. After 24...

陳盈廷, Chen, Ying Ting

1982年《聯合國海洋法公約》確立了專屬經濟區的制度後，公海成為海洋生物資源養護與管理的一大缺口，特別是在養護與管理跨界及高度洄游魚群上產生防護的漏洞。1995年國際間通過《聯合國跨界與高度洄游魚類協定》，旨在履行《聯合國海洋法公約》關於養護跨界與高度洄游魚群之規定，並進一步訂立更完善之規範，特別是協定重視各區域性漁業組織之功能，期能透過該些組織，更有效率地管理並養護各區域之跨界與高度洄游魚群。因此，《聯合國海洋法公約》、1995年協定與區域性漁業組織相互間透過法律規範，從架構、實踐與實際執行這三方面試圖形塑一個較為嚴謹及嚴密的網絡。 2004年中西太平洋漁業委員會（WCPFC）成立。協定與委員會透過登臨與檢查的方式，以養護與管理在公海海域之跨界與洄游魚群。然公海自由及公海上由船旗國管轄乃習慣國際法，而為在保育海洋生物資源，在公海上採取相互登臨與檢查為國際海洋法發展之趨勢。由於登臨與檢查乃涉及一國之主權問題，台灣因於國際上之特殊地位，在WCPFC中之登臨與檢查權便為一值得探討之議題。 《WCPFC登檢程序》規定《WCPFC公約》之締約方有相互登臨檢查之權利與義務，而WC...

Su Yu Chung, 蘇昱中

碩士 / 國立體育學院 / 體育研究所 / 88 / A Study of Chain Store Management on Chinese Martial Arts:The Case of Jenq Tzyy Tai Chi Chuan Ru Yu Tai Chi Academy ABSTRACT The purposes of this study was to investigate the success factors of management on Jenq Tzyy Tai Chi Chuan Ru Yu Tai Chi Academy. Through in depth interview from the top managers of this institute, and questionnaire survey from the participants of this institute (260 samples were asked to answered the questionnaires, whil...

Hsiao Szn Wn, 蕭思文

碩士 / 臺中健康暨管理學院 / 經營管理研究所 / 93 / The study is based on integrated community development experience of Sher Suei Community of Dayan Village, Yuchi Shiang, Nantou County. Past literatures related to integrated community development were collected to study the main affecting factors, and questionnaires were designed and performed against residents of Sher Suei Community. We sent out 120 questionnaires and received 81 valid responses, and the return rate was 62.50%. To understand main affecting fa...

I-Jung Pan, 潘弋戎

碩士 / 嘉南藥理科技大學 / 環境工程與科學系碩士班 / 92 / A large number of substances in daily use are known to mimic natural hormones in animals. These chemicals known as environmental hormones (EHs) or endocrine disrupting chemicals (EDCs) include some pesticides, PCBs, components of food packaging materials and certain alkylphenolic substances which arise from alkylphenol polyethoxylates (APEOs) commonly used in detergents, paints and cosmetics. Sewage treatment plants (STPs) received natural and synthetic EHs fro...

Si Ying Chen, 陳思穎

碩士 / 輔仁大學 / 大眾傳播學研究所 / 94 /

王詩雅

碩士 / 國立海洋大學 / 水產生物技術研究所 / 90 / Mismatched nucleotides in eukaryotic DNA are recognized by heterodimeric complexes of proteins homologus to the bacterial MutS protein. We attempted to study gene expression of MutS homologues in developing vertebrates using zebrafish ( Danio rerio ) as a model system. A novel cDNA encoding a zebrafish MutS homologue, zfMSH2, was obtained by reverse transcription followed by rapid amplification of cDNA ends ( RACE ). The zfMSH2 cDNA has an open reading frame of 281...

Hsin-Jung Feng, 馮欣蓉

碩士 / 國立海洋大學 / 海洋生物研究所 / 90 / The purpose of the study is to understand the effect of thermal seawater discharges on the community structure, diet and mortality of fishes in the waters around the second nuclear power plant in Taiwan. The fishes were sampled from the three different habitats, sand-muddy bottom and rocky habitats and estuarine, which have different distances from the thermal seawater discharges. For the community structure, we use ANOSIM (Analysis of similarities), Cluster and Ordi...

陳彥樺

碩士 / 國立海洋大學 / 食品科學系 / 90 / Summary Zn concentration in the digestive tract tissue of common carp is higher than that in other fish. It was found that this is because Zn was attached to a membrane protein, and the「Zn binding membrane protein」(ZnBMP) might be located on the basolateral plasma membrane of the epithelial cells and surrounding muscle cells which are attached to collagen type Ⅳ. Besides the digestive tract tissue, the Zn concentration in kidney of common carp is also high....

吳萬財

碩士 / 國立海洋大學 / 食品科學系 / 90 / The DNA encoding chicken lung cystatin was ligated into thioredoxin-pET 23a+ expression vector and transformed into Escherichia coli AD494(DE3)pLysS. High level of soluble recombinant thioredoxin-cystatin (trx-cystatin) was expressed in the cytoplasm of E. coli transformant. Comparing with recombinant cystatin (trx-free), a 38.7% increase of inhibitory activity in soluble fraction was achieved by introducing trx fusion protein. The trx-cystatin was purified to electrop...

陳湘穎

碩士 / 國立海洋大學 / 食品科學系 / 90 / Abstract Two consecutive experiments were conducted to investigate the influence of insulin-like growth factor-I (IGF-I) analog (peptide with 10 amino acids, P), insulin (I) and growth hormone (GH) on carbohydrate utilization in juvenile hybrid tilapia, Oreochromis niloticus × O. aureus. Starch and glucose were used as the carbohydrate sources. Eight experimental diets, including starch (S), starch+peptide (S+P), starch+capsulated peptide (S+P+C), starch+cap...

Hui-Yun Tseng, 曾惠芸

碩士 / 中興大學 / 生命科學系所 / 94 / Gene duplication can double the number of genes. One gene may maintain its original function, and the other gene may evolve a new function. Previous studies suggested that gene duplication may increase the species and morphological diversity in teleosts, and therefore isozyme electrophoresis and different genes were used to date the event of gene duplication. Because the basal teleostei (e.g., Elopomorpha) and Chondrichthyes were not included in previous studies, these ...

Yi-Ru Wang, 王一如

碩士 / 朝陽科技大學 / 應用化學系碩士班 / 98 / The phytosterol of Anisomeles indica: β-sitosterol, stigmasterol, at the presently research, people knew that there is not only an effect to lower the cholesterol but also reduce the cause rate of atherosclerosis. Besides, it orders some related of pharmacological activities from the related references, such as HepG2-cells, antiinflammatory effect, immunomodulatory activities, prostate cancer PC-3 cells, thyroid inhibiting, anti-osteoarthritic properties, etc. First...

Ya-Ting Lee, 李雅婷

碩士 / 朝陽科技大學 / 生物技術研究所 / 98 / Anisomeles indica (L.) KUNTZE. (Labiatae) is an annual and biennial herb. In an alternative medicine this plant has been used as damp heat, carminative, stomachic, detoxification, pain relief, rheumatoid arthritis, gastroenteritis and other inflammatory diseases. It is one of the popular Traditional Chinese Medicine (TCM). In the present study, we firstly started with literature reviews on Pentsaology, Pharmacognosy and Pharmacology of A. indica. Then, we collected s...

Hsuan-Mao Wang, 王宣貿

碩士 / 臺灣大學 / 口腔生物科學研究所 / 98 / The Sonic Hedgehog signaling pathway, as well as the Netrin signaling pathway, both play significant roles in early stages of embryonic development. It has been shown that they help building the central and peripheral nervous system. Moreover, as being discovered recently, tissues other than the nervous system can be influenced by their broad effects. According to our previous studies, constructs of plasmid DNA with the IRES (internal ribosomal entry site) system can...

Cheng-Long Wang, 王承龍

碩士 / 臺灣大學 / 生物環境系統工程學研究所 / 98 / Fish communities play a important role in the freshwater ecosystem, and their life-cycles are obviously related to the environmental factors, especially flow conditions. Fish search for appropriate habitats for growing migrating, spawn, and refuge considering current velocity and depth. Therefore, it is necessary to delineate the correlation between the fish distributions and flow conditions. This main issue is to classify the flow conditions in the Datuan stream ...

徐志文

碩士 / 國立彰化師範大學 / 生物技術研究所 / 95 / A full-length cDNA clone encoding Peroxiredoxin 1 (Prx1), Peroxiredoxin 3 (Prx3) and Arachidonate 5-lipoxygenase (ALOX5) were amplified by PCR from zebrafish cDNA library. The deduced amino acid sequence of Prx1, Prx3 showed high similarity with the sequences of Prx1, Prx3 from various species. The deduced amino acid sequence of ALOX5 showed moderate similarity with the sequences of A5LOX from various species. Furthermore, the coding region of zebrafish Prx1 were i...

Chia-yu Yen, 顏嘉佑

碩士 / 大葉大學 / 生物產業科技學系 / 95 / Milkfish ordinary muscles were used as the raw materials for koji-making. The minced meat from the ordinary muscles was mixed with roasted and crushed wheat (30 mesh) at the meat/wheat ratio (w/w) of 1:1 (group A), 1:0.5 (group B), and 1:0.1 (group C). After inoculating with 0.2% spores of koji mold Aspergillus oryzae, koji was prepared in a koji fermenter by incubating at 35-40℃ and over 95% RH for 36 hours. The koji obtained was then fermented under 20% NaCl for ...

Tseng, Chia-Yu, 曾家鈺

碩士 / 國立臺中教育大學 / 環境教育研究所 / 95 / Because some natural, environmental, cultural and industrial resources facilities near the community are combined and managed improperly, in addition of brain drain of community, the resources facilities become almost no commercial and educational effect, and they become the environment malignant tumor of the community instead. This research, in order to put forward the effective solution to the problem, Fingerling-Cottages of Hwa-Tan Township as an example, it was...

Hwang Meng Shan, 黃孟山

碩士 / 國立中興大學 / 食品科學系 / 81 /

Chun-Chung Chen, 陳俊中

碩士 / 中原大學 / 生物科技研究所 / 97 / Protein expression systems are important tools in modern biotechnology. Many expression vectors provided for the different expression system to produce protein have been developed, except the fish cell systems. The promoter is one of the elements for the expression vectors that regulate gene expression on transcription level and have specific for different type cells. To finding the promoter that is optimal in the different cells, CMV, Krt4, Hsp70, beta-actin and Cmlc2...

Yen, Pei-Jane, 顏碧貞

碩士 / 國立海洋大學 / 水產養殖學系 / 83 /

施淑梅

碩士 / 國立海洋大學 / 水產食品科學研究所 / 83 / The pupose of this study was to elucidate the influence of chromic oxide (Cr2O3) inclusion in the diet on carbohydrate utilization and digestibility by juvenile tilapia, Oreochromis niloticus × O. aureus. 　　In experiment I, eight levels of chromium equivalent (0, 1, 2, 10, 50, 100, 500 and 5000 mg/kg) were each incorporated into the basal diet containing either glucose or starch as the carbohydrate source. There were total of 16 diets, each was fed to triplicate gr...

黃銘志

碩士 / 國立成功大學 / 生物學研究所 / 85 / The purpose of this study was to investigate the effect of different dietary lipid sources on growth performance of tilapia. To understand the relationship between the composition of tilapia sarcoplasmic reticular lipids and the enzymic lipid oxidation in sarcoplasmic reticulum(SR) was also a major purpose for this study. Hybrid tilapia (Orechromis niloticus ×O. aureus) with 3 g mean body weights were given diets containing different lipid sources for 14 weeks. Fiv...

林冠宇

碩士 / 國立彰化師範大學 / 生物技術研究所 / 97 / Extracellular superoxide dismutase (EC-SOD) is one of the important antioxidant enzyme that can scavenge superoxide(O2−•) radical in extracellular matrix. In this study, we cloned zebrafish EC-SOD cDNA sequence by RACE-PCR, the deduced amino acid sequence shows higher conservation with more than 60% similarity when compared with other species. The gene was expressed and purified from E. coli. BL21 (DE3) pLysS, and the yield was 170 μg from 200 mL E. coli. culture. ...

Chu-Ling, Hsu, 徐竹羚

碩士 / 國立花蓮教育大學 / 學習科技研究所 / 97 / Phishing has enormous impact on profit as well as nonprofit organizations. This study aims to investigate anti-phishing performance of universities in Taiwan. Analyzing related information on web sites, we identify the input and output factors regarding anti-phishing investment of the organizations. Experts will grade these factors based on predefined criteria. Since subjective and imprecise judgment can be inevitable, experts’ assessments need to be compromised. T...