Ying-Lin Tsai, 蔡瑩霖

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / The aryl hydrocarbon receptor repressor(AHRR) is a new member of the bHLH-PAS superfamily. In mammalian and several teleost species, AHRR acted as a repressor of AH receptor (AHR) which competed with AHR for binding to ARNT, and made it unavailable to AHR. The AHRR-ARNT complex also bound to the xenobiotic response elements (XREs) but did not activate the target genes. In zebrafish, the two AHRR orthologs, AHRR1 and AHRR2, have also been suggested to work in a nega...

Jung-Jiu Wang, 王中櫸

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / Two growth trials were conducted to investigate the effect of dietary protein and lipid on growth and body composition of juvenile tomato　clownfish (Amphiprion frenatus). In Experiment 1, we produced six isoenergetic diets that protein was ranging from 30 to 55%, and use fish meal for protein and cod liver oil for lipid with cellulose and α-starch to adjust the energy. Each diet has triplicate groups of tomato clownfish (initial weight: 0.27±0.01 g per fish) were fe...

Chan-hsin Liu, 劉俊訢

碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 / Osteoporosis is a degenerative bone disease of bone that leads to an increased risk of fracture, and characterized by a reduction in bone mineral density due to a loss of calcium and collagen. Recent studies indicate that collagen possessing a wide range of biological activities including curing rheumatoid arthritis and prevent osteoporosis. Collagen was usually found in chicken and bovine. However, there are misgivings, for example: Avian Influenza and Bovine Spongi...

蘇明偉

碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 /

Hung Yu Hsu, 許紘瑜

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / This study was to find out the effects of dietary YUPE (Yucca plant (Yucca shidigera) extract) on growth, survival, feed efficiency, protein efficiency, oxygen consumption, and oxygen consumption of fry and juvenile of Snubnose pompano (Trachinotus blochii). Experiment I: Juvenile fish were fed diets containing YUPE at 0, 150, and 300 mg kg-1 for 6 weeks. No differences in survival and growth were found among treatments. The resulting fish were kept in tightly ...

Chun-Kai Huang, 黃俊凱

碩士 / 東海大學 / 畜產與生物科技學系 / 98 / Abstract Cytochrome P450 1B1 (CYP1B1) is a monooxygenase which is expressed in eukaryotic endoplasmic reticulum (ER). CYP1B1 protein converts estradiol to 4-hydroxyestradiol by hydroxylation to reduce estrogen activity in animals. CYP1B1 proteins have been achieved catalytic activity in ER membrane, so this study was to use baculovirus expression system to express bioactive CYP1B1 protein. The western blot analysis results showed recombinant CYP1B1 protein was expres...

Hsien-Chieh Chiu, 邱顯傑

碩士 / 國立臺灣大學 / 獸醫學研究所 / 88 / In recent years, viral nervous necrosis (VNN) caused mass mortalities of hatchery-reared grouper larvae in southern Taiwan. The disease was characterized by vacuolation in the spinal cord, brain and retinal tissue in histopathological examination. By using two pairs of specific primers (F1-R3 & F2-R3), the RT-PCR had amplified two predicted DNA fragments (T2 870 bp & T4 421 bp) from RNA extracts of diseased grouper larvae. The homology (78 %-98 %) of T2 fragment to th...

Chang, Kai-Ming, 張凱明

碩士 / 國立臺灣大學 / 漁業科學研究所 / 88 / Abstract In vertebrates, the insulin-like growth factor system (IGF system) plays an important role in early embryo development and postnatal growth. The IGF system consists of the following parts. (1) ligands: insulin-like growth factor (IGF-I and II). (2) binding protein: insulin-like growth factor binding protein (IGFBPs, IGFBP-1 to -6). (3) protease: insulin-like growth factor binding protein protease (IGFBP protease, IGFBPP). (4) receptors: ins...

chia-chang chiang, 江佳昌

碩士 / 國立臺灣大學 / 漁業科學研究所 / 88 / Some freshwater teleosts are capable of adapting to seawater when challenged; however the related energetic and physiological consequences are still unclear. The present study was conducted to examine changes in brain and gills parameters (Na+,K+-ATPase, creatine kinase, creatinine and ATP) and expression of Na+,K+-ATPase and creatine kinase (CK) in tilapia (Oreochromis mossambicus) during acute responses to transfer from fresh water (FW) to seawater (SW). After tran...

Yu-Ming Yang, 楊育明

碩士 / 國立臺灣大學 / 動物學研究所 / 88 / Translational control is very important for oogenesis. Expression of many messages in oocytes is temporally regulated. These messages may not be translated immediately. Instead, they are utilized to produce proteins that participate in the formation of tissues and organs in a particular phase of embryogenesis. Our data indicates that a large amount of the mRNA of chorion components, including ZP2 and ZP3, are transcribed during early stage of oogenesis, yet...

Ching-Shuen Cheng, 鄭清順

碩士 / 國立海洋大學 / 水產生物技術研究所 / 88 / A strong UV-damaged-DNA binding activity was detected in the extracts of zebrafish early embryos ( 3 to 12 hrs after fertilization) The extracts prepared from zebrafish at developmental stages from 12 to 84 hr showed a gradual decrease in binding affinity toward UV-damaged DNA. The extracts of 3 to 12-hr-old zebrafish displayed a preferential binding to a duplex oligonucleotide containing either a cyclobutane pyrimidine dimer (CPD) or a (6-4)photoproduct (6-4 PP), ...

Tin-Hao Wang, 王廷豪

碩士 / 國立海洋大學 / 水產生物技術研究所 / 88 / The partial ORF of superoxide dismutase gene from Vibrio cholerae O1 Ogawa(+), Ogawa(-), and Non-O1 were obtained successfully by PCR techniques using the degenerate Mn-SOD No and Mn-SOD Co primers. The start codon and stop codon of the sod gene from these three Vibrio strains were found using the gene specific primers and random primed gene walking PCR techniques. The full-length sod gene from Vibrio cholerae O1 Ogawa(+), Ogawa(-), and Non-O1 were all composed of ...

Hsieh Yu-Wen, 謝喻文

碩士 / 國立海洋大學 / 食品科學系 / 88 / Abstract The polymerase chain reaction (PCR) technology and sequence analysis were used to determine the amount of genetic variation in a 376 nucleotides region of the mitochondrial cytochrome b gene in fresh, frozen, cooked meat of puffer Takifugu rubripes and fresh, frozen and dried dressed fillets of puffer Takifugu oblongus. It was found that puffer Takifugu rubripes and T. oblongus had their unique genotypes. The range of sequence divergence ...

Ts'ai-Lien Liao, 廖彩蓮

碩士 / 國立臺灣大學 / 動物學研究所 / 87 / Carp elongation factor 1α( EF-1α) and ribosomal proteins( L37 and S17) were isolated from an ovary cDNA library. EF-1α is a 1651bp cDNA and encodes a 458 residue polypeptide. L37 is a 389bp cDNA and encodes 92 residue polypeptide. S17 is a 434bp cDNA and encodes 135 residue polypeptide. They are extremely high homologous to their counterparts in other animals. In this paper, I study the expression change of EF-1α, L37 and S17 during the oogenesis to understand the tra...

Yieh-Her Hsiao, 蕭亦何

碩士 / 國立海洋大學 / 水產生物技術研究所 / 87 / Infectious pancreatic necrosis virus (IPNV) is one kind of the contagious and widespread fish diseases. IPNV belongs to the Birnaviridae family of virus, which has bisegmented ds-RNA genomes and often causes sever damage to the aquaculture industry in Taiwan. The purpose of this study was to investigate whether the expression of hammerhead ribozyme in persistent Tilapia ovary-2 (TO-2) cells can protect the cells from viral replication. Seven target sites...

Cliff, Ji-Fan Lin, 林致凡

碩士 / 國立海洋大學 / 水產生物技術研究所 / 87 / The liver-enriched hepatocyte nuclear factor 1 (HNF1) is an important transcription factor for IGF-I in adult liver. Only one conserved HNF1 binding site was found near the transcription initiation site of tilapia IGF-I gene. To study the role of HNF1 in the signaling pathway of GH-IGF-I axis, degenerated primers designed from known sequences of HNF1 were used to amplify HNF1 partial sequence from tilapia liver mRNA by one step RT-PCR. After gel electrophoresis, RT...

Ging Chung Yu, 俞景中

碩士 / 國立海洋大學 / 食品科學系 / 87 / Abstract The objectives of the study are to explore the feasibility of using same molecular weight (Mw), but different degree of deacetylation (DD) or using different Mw but same DD water-soluble chitosans to prepare active package with antioxidation activity to be used in mullet roe storage. Chitin was prepared from shrimp (Solenocera prominentis) processing waste by chemical process and then alkali (140℃, 50 % NaOH) deacetylation for various time to obtain...

高嘉惠

碩士 / 國立海洋大學 / 食品科學系 / 87 / The heating effect of Far Infrared (FIR) radiation with circulating air was determined by thermal diffusivity, absorptivity and convection heat transfer coefficient. Result from computer simulation shows that the thermal diffusivity has limited effect on the temperature increase comparing to the absorptivity for the geometry of thin fish slices. Surface convection heat transfer coefficient is another key factor. Absorptivity were calculated by matching simulation and e...

Ming-Shyong Wang, 王明雄

碩士 / 國立海洋大學 / 食品科學系 / 87 / Zinc concentrations in most tissues of animals and fishes were around 10~50 ppm; however, those in the digestive tract tissue of common carp were found to be extraordinarily high, being 300-500 ppm. It is also known that the nuclei/cell debris fraction was the major fraction responsible for these high concentration of zinc. In order to survey the specificity and affinity of zinc binding substance(s) in the digestive tissue of common carp, 65Zn was added to the Debris F...

Huang Ting-Shih, 黃丁士

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / Production of live food organisms is the key to the success of larval rearing. Culture duration and media and nutrition of the food fed to the live food organisms all can affect the quality of the live food organisms, which in turn affects the health and survival of the larva. Rotifer is an important live food organism for marine fish larva. However, HUFA (Highly Unsaturated Fatty Acid) content of rotifer is usually not sufficient to satisfy the nutritional requiremen...

李佳育

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / Aeromonas hydrophila strain AH20 was isolated from Corydoras aeneus which shown the symptom of hemorrhage. A plasmid, pPH4, which carry a unique aerolysin sequence was labeled by DIG and used as a probe to hybridize strain AH20 chromosomal DNA. Homologous DNA residing in the AH20 genome could not be detected. Enzymatic activity of either protease or phospholipase C, both are postulated to be virulent factors to destroy red blood cell (RBC) membrane and consequently re...

Yi-lun Tsai, 蔡伊倫

碩士 / 國立中山大學 / 海洋生物研究所 / 89 / Abstract This study uses different morphometric methods to study morphological variation in the population of Engyprosopon grandisquama near the southwestern coast of Taiwan. In addition to the traditional multivariates method , geomorphmetric methods, including the Baselined method and General resistant fit method were used and the results were compared. Results from ocular-side and blind-side analysis are similar. Appearance among locations is different. Overall, ...

Chen, Rong Zong, 陳榮宗

碩士 / 國立臺灣大學 / 漁業科學研究所 / 82 /

Cheng-Yao Yang, 楊程堯

碩士 / 國立臺灣師範大學 / 生命科學研究所 / 102 / I. The Expression Pattern of Trip6 in Mouse Brain Trip6 (Thyroid receptor-interacting protein 6) is a focal adhesion molecule and its function is related to cell adhesion, cell migration and gene transactivation. Trip6 has been identified as a “stemness” gene which expresses higher in different lineage of stem cells. To investigate the role of Trip6 in neural stem cells, we examined the expression level of Trip6 in the brains of embryonic and adult mice, and found...

Meng-Chuan Hsieh, 謝孟娟

碩士 / 國立嘉義大學 / 食品科學系研究所 / 102 / The purpose of this study was to investigate the effect of purple yam dietary supplement on the growth parameters and antioxidant activity of juvenile hybrid tilapia, Oreochromis niloticus × O. aureus. All of 405 juvenile hybrid tilapia with 0.95g mean body weight were distribute to 27 aquarium. The fish were divided into nine groups, control group, low (10% ; F10%) and high (28%; F28%) yam flesh groups, low (5% ; FE5%) and high (10% ; FE10%) 50% ethanolic yam fles...

張瀛玹

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 102

Ming-Hsiang Hsueh, 薛名翔

碩士 / 崑山科技大學 / 企業管理研究所 / 103 / The study is to explore the business growth and strategic management of four mullet processing enterprises in Kaohsiung by employing Yin’s (1996) qualitative case study. It tends to discover how these firms to face globalization and develop effective strategies for business growth in the very traditional local business with geographic boundary. The study conducts "Structure-Conduct-Performance (SCP)" framework for research model. First, the study investigated the b...

Ya-Ging Liu, 劉雅菁

碩士 / 國立彰化師範大學 / 生物技術研究所 / 101 / Thioacetamide (TAA) is a hepatotoxin that can cause hepatocellular carcinoma in mice and zebrafish, but little is known about its toxic effect on embryonic development. In the previous study, we have showed TAA can enhanced ROS generation and induced the edema in pericardial sac (eps) formation, but the cellular mechanism was still unclear. In this study, we try to identify the cells which involved in TAA induced eps formation, and overexpression of manganese supe...

Shu-Yu, 劉書瑜

碩士 / 中山醫學大學 / 生物醫學科學學系碩士班 / 99 / CUG-binding protein 2 (CUGBP2) is a member of the CUG-binding and ETR-3 like factors (CELF) protein family, and functions as mRNA alternative splicing regulator. Previous studies have shown that CUGBP2 is a trans-acting factor associated with human diseases, such that its mis-regulation results in aberrant splicing of various genes. Due to the alternative splicing regulation activity of CUGBP2, its function is also very important in fetal development. Orthologous...

Yu-Sheng Deng, 鄧育昇

碩士 / 中國醫藥大學 / 生態暨演化生物學研究所碩士班 / 99 / In order to understand the metal accumulation and detoxification in tilapia (Oreochromis mossambicus) under various chronic copper (Cu) exposures, we investigated the subcellular distributions of Cu. The tilapia were exposed to 0, 0.1, 0.3, and 0.6 ?慊 ml-1 Cu for 28 days followed by a depuration biosaays of 7 days in water. The relative Cu distribution between the metabolically active pool (MAP) and the metabolically detoxified pool (MDP) were analyzed. The bi...

Hsu,fukai, 許富凱

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 102 / The aim of this study was to investigate the effect of different dietary proteins on the growth and postprandial changes in free amino acids in the blood of tilapia. The growth performance and free amino acids analyses were performed in the first, fourth and eighth week of the growth trial respectively. In the growth trial, three isonitrogenous and isocaloric experimental diets containing extracted soybean protein ( ESP ), defatted fish protein ( DFP ) and cas...

Yu-Huan Hsu, 徐于歡

碩士 / 朝陽科技大學 / 應用化學系生化科技碩博士班 / 102 / Activated T lymphocytes play a pivotal role in the pathogenesis of cell-mediated autoimmune diseases and chronic inflammatory disorders. T cell triggered by the activation of the T cell receptor/ cluster of differentiation 3 (CD3) complex and costimulatory receptor CD28 leads to the expression of cytokines such as interleukin-2 (IL-2), IL-4 and IL-13. Transcription factors nuclear factor of activated T cells (NFAT) and nuclear factor-kappa B (NF-κB)play essent...

Jie Pang, 龎潔

碩士 / 國立高雄海洋科技大學 / 水產養殖研究所 / 102 / This study evaluated the effects of carotenoids and methyltestosterone on the coloration and growth of red tilapia (Oreochromis sp.). Dietary supplemented with three pigment sources: Arthrospira platensis (10g/kg, AP diet ), oleoresin paprika (10g/kg, OP diet ) and astaxanthin (0.2g/kg, AS diet ). The control diet (CO diet) was no supplementation pigments. In addition, four above-mentioned diets were added with methyltestosterone (10mg/kg) as MAP, MOP, MAS, ME d...

吳敏華

碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / Both clam (Corbicula fluminea) and Chlorella sorokiniana have antioxidative and hypolipidemic activities. The purpose of this study was to elucidate the combination effect of freshwater clam and Chlorella sorokiniana on antioxidative, prolongation of LDL oxidation and preventive of atherosclerosis using tilapia as an alternative animal model. Freshwater clam and Chlorella sorokiniana were extracted with hexane, ethyl acetate, ethanol and water. The in vitro model, s...

吳雅蓉

博士 / 國立臺灣海洋大學 / 食品科學系 / 100

Ahmad Syafiq Ahmad Nasir, 曼沙斐

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 100 / The effects of different dietary protein levels and crystalline amino acids supplementation in plant-based protein diets (soybean protein concentrate and wheat gluten) on growth performance and ammonia excretion of Nile tilapia (Oreochromis niloticus) was studied. There were eight diets containing different levels of plant-based protein (20%, 25%, 30% and 35%) supplemented with essential amino acids (lysine and methionine) namely LM and non-essential amino acids (a...

Ming-Hung Wu, 吳明鴻

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 100 / Two iridoviruses, grouper iridovirus of Taiwan (TGIV) of genus Megalocytivirus and grouper iridovirus (GIV) of genus Ranavirus, have caused serious epidemics in several maricultured fish species in Taiwan. In this study, we used de novo transcriptome analysis and Digital Gene Expression (DGE) technology to investigate the gene expression profile changes in orange-spotted grouper (Epinephelus coioides) spleens after TGIV and GIV infections, separately. After DGE, th...

Wei-Kai Liao, 廖偉凱

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 100 / The transgenic yeast strain LZ-8 was added in feed which according to different protein contents (1.25, 6.25, 12.5 and 37.5 mg LZ-8 protein/kg) feeding grouper (Epinephelus coioides). After fed with feed contains LZ-8 for 1, 3, 5, 7 and 14 days, groupers were injected with NNV and the survival rate was recorded during the experiment. 9 orange-spotted groupers were sampled after 14 days. The results of NNV challenge experiment, 37.5 mg LZ-8 feed fed for 1 day had th...

李舒萍

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 100 / This study evaluated the effectiveness of oral and injected vaccines prepared from formalin-killed Streptococcus iniae cells, as well as the protective effect of Epinephelus coioides fed with egg yolk immunoglobulin (IgY) from hens pre-immunized with the same vaccine-supplemented diets against Strep. iniae infection. In this experiment, the median lethal concentration (LD50) of the bacterium strain LW2010, was 1.89×105 CFU/fish body weight. The symptoms of Streptoc...

Hui-Yen Chin, 陳韋燕

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 100 / Muscle-specific transgenic zebrafish over expressing giant grouper’s growth hormone was established to study the autocrine and paracrine effect of this hormone in vertebrate system. Growth enhancement was observed in the transgenic zebrafish analyzed by phenotype and gene expression analysis. The transgenic zebrafish showed an increase in body weight up to 92% and total body length up to 31% as compared to control. In addition, hypertrophy was observed in the musc...

Hsin-Tien Yuan, 袁心恬

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 100 / This study uses in vitro and in vivo experiment to investigate the effect of Chinese herbal compound (Xiao-Chai-Hu-Tang) on growth and non-specific immune parameters of grouper (Epinephelus coioides). In vitro experiment in this study uses various doses (0、0.1、0.5、1、2、4、8、10 mg/ml) of Xiao-Chai-Hu-Tang extract for the in vitro culture of orange-spotted grouper leukocytes. The result showed that leukocytes of grouper culture with over 1 mg/ml of Xiao-Chai-Hu-Tang ha...

Yu-Ling Peng, 彭于綾

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 100 / Spermidine/spermine acetyltransferase (Ssat), which belongs to GCN5-related N-acetyltransferase (GNAT) superfamily, catalyzes an acetyl-transferring reaction from acetyl-CoA to primary amine substrates. Two ssat isogenes, SSAT1 and SSAT2, were found in human. The substrates of human SSAT1 are spermidine and spermine, thus it regulates polyamine homeostasis. On the other hand, human SSAT2 cannot catalyze polyamine catabolism. Previous reports have indicated that th...

Tzu-Yun Huang, 黃子芸

碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / Ornamental fish are vulnerable to stress during transportation. The aim of this study was to create a natural anti-stress drop for guppy to increase stress tolerance in transport. The main objectives are the following: (1) to design a simulated transport model to evaluate the effect of GTE (Glycine tomentella) extract drop in packing water of guppy (Poecilia reticulate); (2) to standardize the application method of using GTE drop during logistics; (3) to identify th...

Yao-Jang Wang, 王耀徵

碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / The purpose of this study was to identify lipid-soluble and water-soluble functional compounds of sorghum distillery residue (SDR) extracted with different solvents. Tilapia was used as an animal model to evaluate the effect of different functional compounds on cold-adaptation, and to validate the efficiency of the antioxidant compounds extracted that may be responsible for the anti-cold stress effect of SDR. The best extraction solvent was 95% ethanol. Higher func...

林容正

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 100 / The purpose of this study was to investigate the choline metabolism in hybrid tilapia（O.aureus (♀) × O. niloticus (♂)） by two input methods with urine collection, in order to determine the absorption and utilization through the 24 hour course of choline concentration variation in the blood and urine. There were two experiments the forced feed 0.3％ body wet weight ration level experiments and injection experiments. The forced feed groups of fish were feed orally adm...

Cheng, Chao-Jung, 鄭肇融

碩士 / 國立澎湖科技大學 / 觀光休閒事業管理研究所 / 102 / In recent years, under the local government vigorously promoted, many cities and counties to take local industries patterns tourist festival strategy, hoping to use culture, festivals and other ways to attract visitors to participate, and thus enhance local awareness to promote local economic development. The festivals are usually local characteristics and most influential industry in the region as the main object of this study clove fish culture season Chikan...

Hsin-Ping Chien, 簡欣屏

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 101

Huan-Feng,Lin, 林洹峰

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 101 / To lower the cannibalism of grouper fry in indoor nursery farm, grading and net cage are used widely, but the cages block water flow function of removing feces and residue feed. Therefore, intensive labor and time are consumed to clean those waste and change water to avoid spoiled water quality and decrease the possibility of disease outbreak. The laboratory has developed an air left solid substance collector and a rotation solid substance collector powered by wate...

Lee, Kuan-Hsien, 李冠賢

碩士 / 國立交通大學 / 理學院科技與數位學習學程 / 101 / This research aims to investigate the effectiveness and feasibility of the teaching strategy for anti-phishing education, which integrates the concept map approach into tablet PCs to enhance student motivation. A total of 155 students from a junior high school in Taipei city participated in this study. After the students used tablet PCs and learned the basic concept of anti-phishing from the instructor, the students were randomly divided into the control and th...

CHUNG-CHING HSIAO, 蕭崇景

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 100 / microRNA miR-In300 is an intronic miRNA which is located within the first intron (intron-1) of the zebrafish myogenic factor 5 (myf5) gene. When the transcription of myf5 is decreased in 32 hours post-fertilization (32 hpf), miR-In300 is still detectable in brain and muscle until 7 days post-fertilization. We suspeat whether the remnant miR-In300 is able to regulate gene expression and to affect zebrafish myogenesis. To address this issue, we choose the 32 hpf z...