Ming Yuan Lee, 李銘遠

碩士 / 弘光科技大學 / 化妝品科技研究所 / 102 / Alginate is a widely used polymer in many fields due to several advantages such as easy availability, cost effectiveness, biodegradability and biocompatibility. Although sphere is the common shape of alginate beads, the shape and size are important factors for applications. There are two experiments in this thesis. The first experiment investigated effects of crosslinker type (CaCl2, BaCl2), crosslinker concentration (1, 10 wt/v %) and temperature (8°C, 25°C) on al...

Yu-Tang Chang, 張育堂

碩士 / 國立中興大學 / 生物科技學研究所 / 101 / Diatoms are single-cell algae found in almost all types of waters. The goal of this study is to establish a system that includes efficient ways to collect diatoms from natural water sources, to isolate them into single species, to identify and classify them and to store and preserve them. Diatoms were collected from marine water sources collected from a coral rock found in Kenting Area, Pingtong County, Taiwan and separated into single-cell diatom cultures using a ...

Keng-Ming Wu, 吳耿銘

碩士 / 國立中興大學 / 生物產業機電工程學系所 / 103 / The purpose of this study was to develop a microwave sterilization system used to improve time consumption and resolve the chemical residues problems of microalgae culture medium treated by the traditional autoclave and sodium hypochlorite methods, respectively. The feasibility experiment of continuous microwave sterilization using microalgae suspension combined with five species of microalgae, Isochrysis galbana (CCAP 927/1), Porphyridium purpureum (CCAP 1380/3...

WU,LING-LING, 吳玲玲

碩士 / 國立清華大學 / 輻射生物研究所 / 79 /

CHEN, ZHONG-XIN, 陳忠信

博士 / 國立臺灣大學 / 政治研究所 / 74 /

GAO, WEN-YUAN, 高文媛

碩士 / 國立臺灣大學 / 地質研究所 / 74 /

Chi-Han Kao, 高啟瀚

碩士 / 中原大學 / 生物環境工程研究所 / 103 / With great industrial developments in Taiwan, raw materials and plenty of compounds are used in the processes which result in more complex compositions of wastewater. In fact, excess nitrogen-containing compounds can cause water pollution, such as Eutrophication and algal blooms. Actually, our government had promulgated the standards of ammonium concentration in wastewater discharging to prevent our environment from damaging. In the other hand, compared to corn and ...

CHEN, ZU-DE, 陳祖得

碩士 / 國立臺灣大學 / 農業化學研究所 / 76 /

洪瑋婷

碩士 / 國立海洋大學 / 食品科學系 / 91 / Abstract The objective of this study is to explore the physicochemical and rheological properties of chitosan — sodium alginate dilute solution. The dilute solution is definded as solution’s concenation lower than reciprocal of their characteristic concentration(c*). The characteristic concentration of chitosan and sodium alginate in acetic acid solutions were determined from their intrinsic viscosity([η]) by capillary viscometry. Chitin was extracted fr...

WANG SIN PING, 王新評

碩士 / 大葉大學 / 環境工程學系碩士班 / 102 / Microalgae can be easily cultivated, produce a variety of active substances and pigments , absorb carbon dioxide and heavy metals and can be used for biomass energy production . The effect of medium composition on the growth of Nannochloropsis sp. was investigated in batch cultures using the modified Walne medium containing different ingredients. The results of the study include : 1. Effect of salt concentration on the growth of Nannochloropsis sp. The higher growt...

Lin, Hui-Chu, 林惠珠

碩士 / 明新科技大學 / 土木工程與環境資源管理系碩士在職專班 / 103 / Organic ingredients containing diatomite filter cake (hereinafter referred to as waste diatomite) is waste produced from epoxy process. Landfills is the current major disposal of this type of waste which is high cost and not environmentally friendly . This study was to explore the use of high-temperature pyrolysis treatment to remove the organic matter contained in waste diatomite, and recycling used in the epoxy filtration process . The samples were coll...

Yu-Shan Huang, 黃于珊

碩士 / 淡江大學 / 水資源及環境工程學系碩士班 / 103 / Phenolic compounds are commonly used in many industries, which discharge into wastewater with the characteristics of toxic and low biodegradability. Physical or chemical treatments are costly and easily generate secondary pollutants, while biological methods produce large amounts of waste sludge, which cost much during the final disposal. Microalgae have been proven to have the ability to remove the organic carbon, nitrogen, and phosphorous in the wastewater, an...

Chih-cheng Yang, 楊智誠

碩士 / 國立臺灣大學 / 動物學研究所 / 96 / Brevetoxins (PbTx) are the major neurotoxin produced by algae when red tide happens and are classified according to their chemical structure named Brevetoxin-A(PbTx-A) and Brevetoxin-B(PbTx-B). Researches in the past believe that these toxins bind to Na+ channel and affect its activation and inactivation. Reports in the past focused on the classification, purification and detection of the toxin. For the electrophysiology study, crafish and squid giant axon were used a...

Yang, Yao-Houng, 楊曜鴻

碩士 / 大葉大學 / 生物產業科技學系 / 96 / This thesis can be divided into three parts. In the first part, Spirulina tinctures were prepared by soaking Spirulina powder in ethanol solutions of different concentrations. The optimum soaking concentration and soaking time were studied by sensory evaluation and the analysis of the yields of some functional components in the tinctures. In the second part, fermented Spirulina wines were prepared using a commercial yeast product (Red Star Distiller’s Active Dry Yeast...

Ya-lan Hu, 胡雅嵐

碩士 / 國立中央大學 / 環境工程研究所 / 97 / Microcystin is a common toxin in the eutrophic lakes and reservoirs. However, the mechanism of the transportation of microcystin in lakes and reservoirs, especially, in micro-environment of the soil/water system is not yet clarified. The adsorption and photodegradation of microcystin poisoning could adversely affect the environment. Hence, the main purpose of this study was focused on the major factors that affect the transportation and fate of microcystin in natural...

SHEN-JHONG CHEN, 陳信中

碩士 / 國立臺灣科技大學 / 化學工程系 / 97 / Cyanophycin is a water-insoluble reserve polymer of cyanobacteria, and is a product of nonribosomal peptide synthesis. We used recombinant Escherichia coli to produce cyanophycin, and extracted cyanophycin from the cells by the acid extraction method, and then analyzed its amino acids composition by high performance liquid chromatography. In order to increase the cyanophycin yield, we supplemented additional amino acids in the medium when induction. After the additi...

Shu-Jyuan Li, 李淑娟

碩士 / 國立東華大學 / 海洋生物科技研究所 / 97 / During a stable symbiosis, the number of Symbiodinium sp. cells residing in a gastrodermal cell of Cnidaria is the same. However, the mechanism of how the cell proliferation of symbionts is regulated remains unclear. The cell proliferation of Symbiodinium sp. in symbiotic Aiptasia pulachella and the protein expression of its CDK A were investigated. In addition, the effects of various light conditions on the cell proliferation of cultured Symbiodinium sp. (both cla...

Chih-Hao Hsu, 許之顥

碩士 / 淡江大學 / 水資源及環境工程學系碩士班 / 102 / Activated sludge is popular method of biological treatment in the sewage management. However, the operation process confronts some problems which would produce lots of CO2, increase large amount of sewage sludge and is difficult to find the final disposal site in Taiwan. Many studies have proven the microalgae can remove carbon, nitrogen and phosphorus of the sewerage and fix carbon dioxide simultaneously. Furthermore, the proliferated microalgae form the treatm...

Chi-Chen Tsai, 蔡其成

碩士 / 崑山科技大學 / 環境工程研究所 / 102 / Cladophora genus is widely distributed in the world's freshwater and marine waters of large benthic epiphytic algae, this study Taiwan's two kinds of widely distributed in estuarine Cladophora ,Cladophora. glomerata and Cladophora. vagabunda observed at different tidal estuary the river algae in vitro extracellular substance (extracellular polymeric substance, EPS) formed clayey layer (mucilage layer) thickness and epiphytic algae on algae (epiphytic algal) changes ...

Yu-han Huang, 黃毓涵

碩士 / 國立成功大學 / 化學工程學系碩博士班 / 97 / Microalgae have received much attention as renewable energy resources, since the photoautotrophic mechanism can convert the atmospheric carbon dioxide into biomass. In order to harvest microalgae with the high productivities of biomass and lipid, design of experiments with regression analysis was applied to optimize the culture conditions of medium. Under the mentioned optimal culture conditions, a continuous culture process was purposed to carry out for harvestin...

Jing-Yi Chen, 陳靜儀

碩士 / 國立成功大學 / 化學工程學系碩博士班 / 97 / Polyacrylonitrile (PAN) nanofibrous membrane providing huge surface area could be manufactured by electrospinning as support for enzyme immobilization. The enzyme, Aspergillus niger cellulase was immobilized on electrospun PAN nanofibrous membranes by amidination reaction for long term operation. In addition, covalent bond formation between enzyme molecule and the nanofiber was confirmed from FT-IR measurement. The optimal operation conditions of cellulase immobil...

Yi-Chen Shiu, 徐意淳

碩士 / 國立成功大學 / 地球科學系專班 / 96 / Uranium- and thorium-series have a series of radioactive elements with different half lives. Because the parent nuclides (238U, 234U) have higher solubility than the daughter radionuclides (234Th, 230Th), when ground water flows through the rocks, the uranium isotopes are preferentially transported in natural waters, such as in rivers, lakes, seas. Meanwhile α-recoil effect would cause 234U/238U activity ratio in waters to be greater than 1. Organisms who inhabit nat...

CHANG-HSIN YU, 張欣宇

碩士 / 長庚大學 / 基礎醫學研究所 / 96 / The Gram-negative bacterium, Klebsiella pneumoniae (KP), is an opportunistic pathogen. In Taiwan, KP associated liver abscess has recently become an important emerging infectious disease. Our previous studies revealed that the KP strains isolated from diabetic patients with liver abscess exhibited greater virulence than that from urinary tract infection patients in mice survival test. We also found the capsule of liver abscess-causing KP contained prominent amount of f...

SURYANI, 林佳佳

碩士 / 國立清華大學 / 生物技術研究所 / 93 / Sedoheptulose-1,7-bisphosphatase (SBPase) is an enzyme unique to Calvin cycle and is involved in photosynthetic carbon fixation. Gene encoding the chloroplast SBPase from Chlorella pyrenoidosa 211-8b was cloned. The coding region of this gene contains 7 exons and 6 introns. The full length cDNA is 1572 bp, with an open reading frame of 1197 bp, which can be translated into a protein with 389 amino acid residues. Comparison of the deduced amino acid sequence reveals 7...

Yu-Yun Lin, 林育筠

碩士 / 國立暨南國際大學 / 生物醫學科技研究所 / 94 / Tyrosinase catalyzes the rate-limiting reaction of melanogenesis. The formation of melanin is regulated by the activity or the expression of the enzyme. Although the whole picture of the signal transduction involved in melanogenesis remains unclear, it is suggested that cAMP plays an important role in the biosynthesis. cAMP modulates melanogenesis in three aspects. Firstly, cAMP activates the expression of microphthalmia associated transcription factor (MITF), wh...

Yu-Ting Su, 蘇郁婷

碩士 / 國立成功大學 / 環境工程學系碩博士班 / 94 / Microcystins, their associated toxin producers, and a few algal metabolites in three typical drinking water systems were monitored and interpreted for their relationships in this study. Two conventional and one advanced water treatment plants (WTPs) were examined, in which Shi-Men WTP represents typical conventional processes, Tai-Lake WTP represents conventional processes with flotation and slow sand filtration, and Chen-Chin Lake (CCL) WTP represents advanced t...

Chih-Tung Chiu, 邱智東

博士 / 國立陽明大學 / 醫學工程研究所 / 95 / Collagen and alginate are two of the most important biodegradable and biocompatible macromolecules used for biomedical applications. The development of new methodology for the modification of these biopolymers is vital to the design of tissue scaffolds. The objectives of this study include the developments of (1) alginate wound dressings modified with polyethyleneimine and ethylenediamine, (2) crosslinking process for hydroxyapaite (HAp)/collagen microsphereic bone g...

Chih-Yu Wang, 王智昱

碩士 / 國立中興大學 / 化學工程學系所 / 95 / Abstract Various light-emitting diodes (LEDs) with different light wavelengths and illumination intensities were employed to explore the effects of light source on photoautotrophic cultivation of Spirulina platensis. From the experimental results, red LED exhibited the highest specific growth rate 0.40(d-1) under the condition of 3000 μmol m-2 s-1. Blue LED showed the least efficiency in the conversion of photon to biomass. Hence, a modified Monod model was proposed ...

Ji-Jheng Huang, 黃基政

碩士 / 國立高雄應用科技大學 / 化學工程與材料工程系碩士在職專班 / 104 / Allophycocyanin, existing in several algaes, could be applied in fluorescent probes and diagnostic kits. In order to developing a simplified purification process of allophycocyanin, the adsorption of allophycocyanin by a magnetic polyethyleneimine composite (Fe3O4@SiO2/PEI) was investigated in this work. The adsorption isotherm analysis demonstrated the adsorption of allophycocyanin followed Langmuir isotherm model. The optimum condition for adsorbing a...

Tin-Yao Chou, 周廷耀

碩士 / 國立中山大學 / 海洋生物研究所 / 91 / The concentration of carbon dioxide in natural seawater is sufficient for microalgal growth, but insufficient for high algal-density culture due to limitation of photosynthesis in the artificial medium. This study was adjusted the initial pH to 5.5, 6.5 and 7.5 by adding CO2 or HCl in 1L flask cultured stagnantly with continuous illumination. The best growth of Isochrysis galbana was found in the culture (CO2-6.5i) with initial pH 6.5 using CO2 adjustment and maintai...

Yuting Ko, 柯瑜婷

碩士 / 國立彰化師範大學 / 生物學系 / 91 / This paper reports the physiological change of various developmental stages and the dought-tolerance mechanism due to stress in synchronized Chlorella which under intermittent illumination of 14 hr light and 10 hr darkness. Protein level obviously increased at the beginning of the light period. Cells began to synthesize starch, soluble sugar and sucrose after 8 hours illumination, and accumulate to maximum at the 16th hour of the cell cycle. Cell volume and fresh weig...

陳政國

碩士 / 國立中興大學 / 植物學系 / 90 /

Ed-Haun CHang, 張義宏

博士 / 國立臺灣大學 / 農業化學研究所 / 89 / Increased concentration of carbon dioxide in the atmosphere is considered to be one of main causes of global warming problem. Among various approach methods for CO2 reduction, biological CO2 fixation by microalgal photosynthesis has been proposed as an economically feasible method. To investigate the biological fixation of carbon dioxide, more than 200 microalgae isolates were screened from the lakes, ponds, sediments, hog wastewater, paddy fields, hot spring and sea...

Yi-Chun Hsieh, 謝宜君

碩士 / 國立中央大學 / 光電科學與工程學系 / 103 / The main purpose of this study is to propose a new method to measure the reaction of photosynthesis and respiration of microalgae to determine whether the light is good for the growth of microalgae when it’s lighted by different spectrum of LED light sources. In our experimental set up we use optical microscope and digital camera to capture the video of chlorella, and we choose the peak of absorption spectrum of chlorella in blue, red and yellow LED light sources,...

Lee-Kuo Kang, 康利國

碩士 / 國立中興大學 / 植物學系 / 89 /

Wang, Yun Shen, 王永昇

碩士 / 輔仁大學 / 生物學系 / 89 / Abstract The utilization of carbon sources by the green algae Chlorella sp. isolated from the Yuanyang lake was studied. The growth of algal cells in the presence of concentrated lake water containing organic carbons was compared with those cultivated with CO2 as the sole carbon source ( i.e. autotrophic ). Analysis of the δ13C in the cells ascertained that algal cells grew heterotrophically when culture medium was depleted in CO2 . In the case, values of cel...

Chen, Horng-Ming, 陳弘民

博士 / 國立臺灣師範大學 / 生物學系 / 84 / The prokaryote Synechococcus RF-1 exhibits circadian rhythms of nitrogen fixation and leucine uptake after being entrained in a diurnal light/dark regimen. The rhythms can also be established by small temperature changes, in either a raised- or a lowered- temperature cycle, within the growth permissive range. At the biochemical level, the synthesis of more than ten of its ...

Chin-Jen Liu, 劉志仁

碩士 / 東吳大學 / 微生物學系 / 83 /

Tseng, Yuh Hua, 曾玉華

碩士 / 文化大學 / 生物科技研究所 / 83 /

Pen, Shih-yi, 彭世宜

碩士 / 東吳大學 / 微生物學系 / 85 /

Lin, Xiao-Zhen, 林筱蓁

碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 103 / Fucoidan is a sulfated polysaccharide and has been demonstrated having a variety of immunomodulatory effects, such as promoting activation of T cells and enhancing anti-tumor responses, inhibit cancer cell drug efflux pump of multidrug resistance. Cisplatin is a widely used as chemotherapy drug in the treatment of human solid tumors. Thakral et al. showed that the interaction of the platinum (II) atom with the negative charge of the anion polymer could cause s...

Hung, Chien-Fei, 洪千斐

碩士 / 國立臺灣海洋大學 / 食品科學系 / 103 / Algae are widely distributed throughout global waters, and the Phaeophyta possess a polysaccharide called fucoidan which has many bioactivities. Fucoidan is water-soluble, and its main components are fucose and sulphate groups. The bioactivities of fucoidan are associated with its structure, monosaccharide composition, the content and the location of sulphate groups, and its molecular weight. The purpose of this study is to investigate the best method for extracting...

Tsai Chih Cheng, 蔡智成

碩士 / 崑山科技大學 / 環境工程研究所 / 104 / Produced by microbial extracellular polymeric substance(EPS) has been regarded as an important renewable resources and technology in recent years, many studies exploring valuable EPS, and has a production potential of algae. Spirogyra spp. is a genus of filamentous green large algae commonly found in fresh water. Under the natural environment, Spirogyra spp. secretes large amounts of EPS and forms a sticky mucilage layer. This study discovered that salinity increa...

Yi-Huei Cheng, 鄭伊惠

碩士 / 國立屏東科技大學 / 水產養殖系 / 94 / Benthic diatoms are the principal food source for postlarval abalone in hatchery; the survival and growth of postlarvae depend on species and density of diatom. How to manage the diatom film is a key issue for successful postlarval rearing. Postlarval abalones have highly survival rates fed by the cultured benthic diatoms, but their settlement on plates and metamorphosis vary with diatom species. The morphology of diatoms could vary with the culture conditions; in ad...

Chiung-Dai Huang, 黃瓊代

碩士 / 大仁科技大學 / 食品科技研究所 / 104 / Microalgae products have been widely used in food, medicines, biomass energy and environmental purposes. In this study, the nutritional compositions, active ingredients and anti-oxidative abilities of microalgae - Chodatella sp. were analyzed, hydrolysis conditions were investigated. Finally, the chodatella sp. hydrolysate was compared with commercial products, such as chicken or clam extracts. The nutritional composition of chodatella sp. contains high amounts of p...

Lin, Lin-Yen, 林伶燕

碩士 / 輔仁大學 / 生命科學系碩士班 / 102 / Nannochloropsis oculata can be considered as a potential biomass for aquaculture, greenhouse gas reduction, healthy food and bio-diesel production. However bacteria, fungi and viruses often caused decreased productivity in large-scale cultivation. The first aim of this survey is using Photodynamic treatment (PDT) techniques to inactive the completely organisms that may interfere the productivity of N. oculata. LED, a cheaply and energy efficiently light source were u...

Guan-Ting Lin, 林冠廷

碩士 / 明志科技大學 / 化學工程系生化工程碩士班 / 103 / In this work, stirred fluidized bed hydrophobic chromatography was employed to purify the C-phycoayanin (CPC) from disrupted Spirulina platensis. To investigate the adsorption and desorption kinetic and thermodynamic studies, the experiments were carried out in a small stirred tank system. The results were further applied to investigate the dynamic adsorption and elution behaviors in small scale packed bed (i.d. 16 mm) chromatography under the various operating...

Chi-Huan Lee, 李季桓

碩士 / 國立宜蘭大學 / 化學工程與材料工程學系碩士班 / 103 / To make use of Taiwan’s natural resources for sustainable development, exploration of abundant water resources (e.g., indigenous microalgae) around Taiwan’s water bodies would be of great significance. This study also chose eastern Taiwan’s water bodies to screen high suctioning microalgal species for high-value product generation via electrochemical impedance inspections. Next, GC analyses upon various biomass could be applied to determine whether the capabi...

CHENG,YU-CHUNG, 鄭有中

碩士 / 中華大學 / 土木工程學系碩士班 / 103 / The purpose of sewage treatment is to reduce the concentration of pollutants in sewage to make its effluents to comply with National Discharge Standards, Taiwan Environmental Protection Agency and not to cause secondary pollution. The quality in design of sewage treatment plant has strong impact on treatment efficiency and stabilization, and the costs of plant set-up and operating. However, domestic wastewater treatment plant design practice is often not based on th...

Kuan-Yu Lin, 林冠佑

碩士 / 中華大學 / 土木工程學系碩士班 / 102 / In today's environment, energy depletion has increasingly become a serious problem. All the countries in the world are, thus, looking for highly effective alternative energy sources. A wide range algae are denoted with its many features. Parts of them can be cultured to produce biomass energy, such as biodiesel with the quality better than one made from the general feed stocks, becoming the key source of supply for today's raw energy demand. This study chose Nannoch...