Chiu-Ti Wu, 吳裘第

碩士 / 臺灣大學 / 海洋研究所 / 95 /

Ko-Ping Sun, 孫可蘋

碩士 / 臺灣大學 / 海洋研究所 / 95 / Microalga, Nannochloropsis oculata, is the commonly useful foodstuff for finfish and shellfish larvae in aquaculture. In this study, we attempt to generate transgenic N. oculata strain which can enhance the quality of larvae. White spot syndrome virus (WSSV) causes severe mortality in shrimp industry. VP28 is a major envelope protein of WSSV and enables to stimulate disease-resistant ability of shrimp. We constructed a plasmid, pCB740-VP28, in which the VP28 cDNA is driv...

Hsin-Hui Wu, 吳欣慧

碩士 / 淡江大學 / 水資源及環境工程學系碩士班 / 96 / The global fossil fuel consumption is demanding and supposed to be used up by A.D. 2050(Ginzburg, 1993) while economy and population are continuously growing. Substitution of the energy resources have been researched for decades including the wind-powered energy, solar energy and the production of biodiesel mainly by agricultural activities. The microalgae have been suggested as one of a good resource of bio-fuel production due to its higher photosynthesis effic...

Tai-Chang Jiang, 江泰成

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / The purpose of this research was to clarify the antioxidant activity of Spirulina platensis water soluble extractive, and the feasibility of utilizing it as cosmetics base. After adding five volumes of 95% ethanol, the hot water extractives from algae was centrifugated at 10000 rpm, 30min and the crude polysaccharides in the precipitation and prolamin in supernatant were separed. The tentative compositions of crude polysaccharides and prolamin were determined. The an...

Hsin-Hui Wang, 王星惠

碩士 / 國立中興大學 / 生命科學系所 / 98 / Spirulina platensis, a blue-green alga, is a rich source of γ-linolenic acid (GLA, 18:3n-6) which has been demonstrated to exhibit many beneficial health effects. GLA from glycerides, especially triglycerides (TG), is absorbed more easily than that from free fatty acids (FFA) or ethyl esters. In the first part of this study, we investigated the effects of light sources and the carbon and nitrogen supply on the growth and GLA content in S. platensis. The algae were cu...

Tzu-Chi Lin, 林子琦

碩士 / 國立臺灣大學 / 食品科技研究所 / 90 / Bifidobacteria is regarded as one of the important probiotics. In this study, Bifidobacterium infantis CCRC 14633 and B. longum B6 were first encapsulated in alginate beads and oil-alginate beads. Susceptibility of these encapsulated bifidobacteria to simulated gastric fluid and viability of the encapsulated bifidobacteria during storage were examined. It was noted that the encapsulation efficiency of both test organisms in alginate or oil-alginate beads w...

HUANG, QING-HUA, 黃慶華

碩士 / 國立海洋大學 / 水產食品科學研究所 / 80 /

Yu-Ting Shih, 施郁庭

碩士 / 國立成功大學 / 環境工程學系碩博士班 / 95 / This research is to investigate the distribution of benthic macroalgae in head waters of Tseng-Wen Reservoir and Nan-Hua Reservoir. Biomass and abundance of macroalage were investigated per month in five sites selected from head water of each reservoir during May 2006 to March 2007. Spirogyra sp. and Cladophora sp. were rarely found in rainy season, May to September, whereas they grew abundantly in other months. Also, their spatial distribution was heterogeneous b...

Dai-Shiuan Lin, 林岱璇

碩士 / 國立嘉義大學 / 水產生物學系研究所 / 95 / The present study was to examine the immune responses of hard clam (Meretrix lusoria, 37 ± 2 mm shell length) that fed different diets of microalage（Chaetoceros muelleri, Tetraselmis chui and mixture of C. muelleri + T. chui or unfed treatments）at different temperatures. Clams that had been acclimated to different temperatures (20℃、25℃or 30℃), were examined for the immune parameters（morphology of haemocytes, total haemocyte counts, different haemocyte counts, pheno...

Shih-En Dai, 戴士恩

碩士 / 國立嘉義大學 / 生物資源學系研究所 / 97 / Streams in the mountainous regions of Taiwan are often modified by series of artificial embankments and low dams to reduce the risk of bank erosion after heavy rain. However, these artificial structures change stream habitats and factors affecting periphyton. The purpose of this study was to study the effects of grazers and nutrients on periphyton in both stream sections. We used microcosm experiments to manipulate grazer and nutrient in the natural and engineered ...

Pei-Shan Wu, 吳佩珊

碩士 / 嘉南藥理科技大學 / 藥物科技研究所 / 95 / Alginate is a natural polysaccharide found in brown algae. Alginate is widely used in the pharmaceutical and food, and has been employed as a matrix for the entrapment of drug. Nicotine has some unpleasant side effects, including bad taste and short half-life for some individuals. It can be used in smoking-cessation therapy and acted as a model drug in the study. The purpose of this study was to develop calcium alginate microcapsules with improved properties for co...

Ming-Larng Hwang, 黃敏郎

碩士 / 國立中興大學 / 植物學系 / 82 / From different stage of the cell cycle using synchronous culture method, Chlorella sp. strain DT cells were desiccated to dry and recultured in liquid medium. Physiological activity of cells from the beginning periods cell cycle was restored. The pigments of the fresh cells and desiccated cells sampled from different time of the cell cycle were analyzed. Only the desiccated cells f...

Chun-Lang Chen, 陳俊郎

碩士 / 國立彰化師範大學 / 生物學系 / 96 / Cadmium (Cd) not only affects human health, but also is toxic to aquatic organisms, inhibiting their growth and changing their physiology and metabolism. Many studies showed that Cd-induced toxicity in plant is linked to lipid peroxidation and increases in malondialdehyde (MDA) content. The green algae Chlorella sp. is easy to culture with a short reproductive cycle, and therefore is a good experimental material. The objective of this study was to examine the effects ...

Tsai, Hung - Chi, 柴宏琪

碩士 / 國立中興大學 / 食品科學系 / 86 / The effects of antimicrobials such as nisin, chelators ( EDTA and HMP ), organic acids ( acetic acid, lactic acid and potassium sorbate ), and their combinationsystems on the growth of E. coli O157:H7 in ground beef were investigated. In addition, the efficacy of immobilized antimicrobials against this gram-negativepathogen by using an edible gel, alginate, as immobilizer was eval...

Yen-Zon Wang, 王彥容

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 /

Chen, Wei-Chen, 陳韋辰

碩士 / 國立臺灣海洋大學 / 食品科學系 / 107 / This study used enzyme-assisted extraction on phycoerythrin (PE) and phycocyanin (PC) from Porphyra dentata, and extracted Porphyra residues by lactic acid fermentation to produce biopreservative phenyllactic acid (Ph-LA). Using 0.5% (w/v) Porphyra powder to induce marine bacteria Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108 to produce curd enzyme, results showed two marine strains could produce better agarose 3.73 U/mL and 1.17 U/mL on 1 day and ...

Chen, Ciao-Yun, 陳巧芸

碩士 / 國立臺灣海洋大學 / 食品科學系 / 107 / This study is aim to investigate purification and storage of phycobiliproteins from Gracilaria tenuistipitata produced by enzyme-assisted hydrolysis and analyze characteristic and bioactivities of mixed agaro-oligosaccharides from Gracilaria tenuistipitata residue. Using G. tenuistipitata to induce marine bacteria Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108 could produce crude enzymes of agarase, amylase, cellulase, xylanase, ι-carrageenase, λ-ca...

LIN, XUAN-HUI, 林宣慧

碩士 / 國立高雄科技大學 / 化學工程與材料工程系 / 107 / The phycobiliproteins are mainly classified into R-phycoerythrin (R-PE), C-phycocyanin (C-PC) and allophycocyanin (APC), which can be applied to fluorescent probe, natural pigment and healthy foods. The traditional purification of phycobiliproteins is complicated and time consuming. The development of adsorption recovery program helps to simplify separation and purification steps. In view of this, this study developed an adsorbent capable of affinity to phycobi...

WANG, YU-CHIEN, 王宇茜

碩士 / 輔英科技大學 / 醫學檢驗生物技術系碩士班 / 107 / Background/Aims: Lung cancer is one of the leading causes of global deaths. Though lung cancer often used chemotherapy in clinical, it still has extremely serious toxicity and side effects on the body. Fucoidan is a polysaccharide extracted from Fucus vesiculosus that has a wide range of pharmacological properties. The purpose of our present study was to investigate fucoidan enhance low concentration doxorubicin to inhibit chemodrug doxorubicin resistance of hu...

Chang, Chen-He, 張慶禾

碩士 / 國立清華大學 / 化學工程學系所 / 105 / abstract hide

蔡騰緯

碩士 / 國立中正大學 / 化學暨生物化學研究所 / 107 / This thesis focus on the synthetic application of α1,3/4-fucosyltransferase from Helicobacter pylori DSM6709 (FucTIII). We have successfully constructed three types of recombinant FucTIII with different expression vectors (pET28a、pTXB1、pMAL-c2X) for protein overexpression comparsion. Among these protein expression systems, pMAL-c2X system with C-terminal (His)6-tag was found to increase the water-solubility of FucTIII with the optimal overexpression yield of 6 mg...

GUO,HUI-RU, 郭惠如

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 106

Shiu, Sin-Yun, 許馨勻

碩士 / 國立臺灣海洋大學 / 食品科學系 / 105 / Fucoidan is a complex sulfated polysaccharide that is found in brown algae. Recently, the diverse biological activities of fucoidan have been studied intensively. In particular, the antitumor activity has recently attracted considerable attention. Fucoidan has also been shown to induce a substantial reduction in viable cell numbers and apoptosis of human colon cancer HT-29 cells. Fucoidan polymers can modulate cell growth in different manners depending on their mole...

Tsai, Sheng-Chieh, 蔡尚杰

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 106 / Nostoc commune is an edible terrestrial blue-green alga in Taiwan. There are many active components in Nostoc commune. But, the study of the effects of Nostoc commune in adipose tissue formation is limited. The aim of this study is to investigate the effects of Nostoc commune on lipid accumulation and its potential of product development. In this study, the Nostoc commune was as an experimental material to prepare Nostoc commune powder (NCP) and Nostoc commune...

Chang, Yu-Pei, 張鈺培

碩士 / 國立高雄應用科技大學 / 化學工程與材料工程系博碩士班 / 106 / Abstract Phycobiliproteins are commonly found in blue-green algae and red algae, and are mainly including C-phycocyanin (C-PC), allophycocyanin (APC), and R-phycoerythrin (R-PE). Owing to their distinguished spectroscopic properties, phycobiliproteins have a wide range of application, such as therapeutic reagents, fluorescent immunoprobes, and health foods. Purification of phycobiliproteins usually involves complex and time-consuming operations, resulting...

Lin, Yi-Ling, 林羿伶

碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 106 / Diatom is a major primary producer in the marine ecosystem. It is also responsible for a part of the global carbon cycle. Its abundant unsaturated fatty acids have the potential to develop commercial products. It is a kind of eukaryotic unicellular algae which is very important for technical applications on marine ecology and biotechnology. Most of the past research has focused on the related basic fields of algae ecology and environmental science. In recent y...

Huang, Lin-Yu, 黃稜育

碩士 / 國立臺灣海洋大學 / 食品科學系 / 106

鄭玉松

碩士 / 國立嘉義大學 / 食品科學系研究所 / 104

CHUNG PEI-YU, 鍾霈諭

碩士 / 國立高雄應用科技大學 / 化學工程與材料工程系博碩士班 / 104

Hsin-Pei Tsai, 蔡欣蓓

碩士 / 國立中山大學 / 海洋科學系研究所 / 103 / 暫不公開

Chih-Hsaun Li, 黎志宣

碩士 / 國立臺灣大學 / 生化科技學系 / 102 / Part 1 Soy is a traditional Chinese food, its protein contains a complete essential amino acids as an equivalent of animal protein. Many studies have also pointed out that soy and soy products have many benefits on human health, including the prevention of cardiovascular disease, kidney disease, diabetes, malignancy, obesity, osteoporosis, allergies, constipation, etc and improved health condition. The purpose of this study is using Lactobacillus paracasei subsp. par...

Huang, Huei-Ru, 黃蕙如

碩士 / 國立臺灣海洋大學 / 食品科學系 / 102 / Cyanophycin is a product from cyanobacteria, which is also called blue-green algae. Cyanophycin is composed by L-aspartic acid and L-arginine in impartial moles. The molecular weight of cyanophycin is around 25-100 kDa. It can be used to produce polyaspartic acids in industry, and can be widely applied in the biomedical and pharmaceutical manufacturing. Our laboratory had previously cloned the gene of cyanophycin synthetase into pRham N-His SUMO Kan Vector, expresse...

Chun-ChihWu, 吳俊志

碩士 / 國立成功大學 / 化學工程學系碩博士班 / 101 / Microalgae have been considered as the promising feedstock for biofuel production, since they can assimilate atmospheric carbon dioxide and produce high value components. Particularly, Spirulina sp. show high contents of phycocyanin, chlorophyll a, β-carotene, polysaccharides and γ-linolenic acid. The microalgae growth is mainly influenced by the temperature, light intensity, nutrients of medium and inoculum concentration, etc. After the cultivation, harvestin...

Huang-Yu Zeng, 曾皇諭

碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / There are many reasons for skin damage like burns, cuts, abrasions and ulcers. Wounds were vulnerable infected by microorganisms if it is exposed to air for a long time. In the recent, the studies focus on accelerating wound repair. It is known that basic fibroblast growth factor (bFGF) promoted fibroblasts migration, proliferation, and keratinocytes growth and effective helped tissue repair and skin wound healing. However, the use of bFGF is limited by two drawback...

Yi-Lang Feng, 馮一郎

碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / The purpose of this thesis is using carrageenase carIII gene from Pseudomonas vesicularis MA103 as a reporter gene combine with plasmid pNZ8149 from nisin-controlled expression system and transgenic to Lactococcus lactis NZ3900 host cells, then evaluate the characterization of nisin-induced k-carrageenase crude enzyme (PV-Car-C), the composition of oligosaccharide (Car-F5) from Pv-Car-C hydrolyzed k-carrageenan of the antioxidant bioactivity and anticoagnlant bioa...

Ding-Chung Wang, 王鼎中

碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / Abstract Our laboratory had cloned the gene of cyanophycin synthetase into pNZ8149 plasmid, and then expressed in lactic acid bacteria to produce cyanophycin, and found that 30℃ is the optimum growth temperature and the optimal induction was carried out under 250 ng/mL of nisin for five hours after the OD600 reached 0.4. However, recombinant lactic acid bacteria grew in M17-L (M17 medium plus 0.5% lactose) medium, and only resulting in low cyanophycine production. I...

Chia-Wen Yeh, 葉佳紋

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 102 / In this study, the effects of high temperature and high pressurepuffing parameters and two extraction methods (A and B) on the extraction mechanism and yield of fucoidan from Sargassum cristaefolium were investigated. Thin layer chromatography(TLC) and Fourier transform infrared spectrometer(FTIR) methods were used toidentify the characteristics of fucoidan and the antioxidant and antitumor activities of fucoidan were also examined. Results suggested thatpro...

Man-Chun Chang, 張曼君

碩士 / 國立中興大學 / 生物科技學研究所 / 103 / Abstract Diatoms are the majority group of unicellular algae in nature. There are more than 100,000 species and approximately 200 genera so far discovered. Our laboratory collected diatoms and seawater from Kenting, the Port of Taichung and the Penghu islands. Almost all had their 18S ribosomal DNA (18S rDNA) sequence compared in BLAST and an optical microscope and Scanning Electron Microscopy (SEM) were also used to observe diatom shell patterns and frustule micr...

Zhen-Hao Liao, 廖振皓

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / The goal of this thesis was to expolore the arsenic methylating ability of marine and freshwater algae. Experimental analysis by hydride generation - packed coldfinger trap - atomic absorption spectrometric (HG-PCFT-AAS) showed that marine alga Tetraselmis chuii cultrued with 0.133�嵱 arsenate for 7 days generated monomethyl arsenic species (MMA) and dimethyl arsenic species (DMA), but freshwater alga Chlorella pyrenoidosa showed no methylating activity under the sa...

Chen,Pin-Wen, 陳品妏

碩士 / 東海大學 / 化學工程與材料工程學系 / 103 / In recent years, with the crisis of fossil energy supply and the requirement of environmental sustainable development, natural resources from fossil, such as petroleum, coal and natural gas, are currently scarce and are to be exhausted in the near future. Moreover, the extensive use of petroleum, coal and natural gas has caused a number of environmental concerns, i.e., the climate change resulting from the global warming effects. Therefore, the development of diff...

Da-Ming Lin, 林達明

碩士 / 國立中興大學 / 化學工程學系所 / 101 / This study investigated ultrasonic solvent extraction, normal phase column elution fractionation and supercritical anti-solvent (SAS) precipitation of fucoxanthin from brown algae of Hincksia mitchellae P.C. Silva. The 5.5 mg/g content of fucoxanthin in algae was evidenced by soxhlet ethyl acetate extraction at 353 K and 16 hours. The 5.4 mg/g content of fucoxanthin of extract was obtained by ultrasonic ethyl acetate extraction at temperature (298 K), solvent to sol...

WAN, YI-CHIEN, 萬宜謙

碩士 / 國立高雄應用科技大學 / 化學工程與材料工程系博碩士班 / 105

Chen, Bo-rui, 陳伯瑞

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 105

Po-Wei Chen, 陳柏維

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 105

Yi-Feng Hsieh, 謝宜峰

碩士 / 國立中興大學 / 獸醫學系暨研究所 / 103 / Protothecosis is an infection caused by unicellular, achlorophyllous, and ubiquitous algae of the genus Prototheca and has been reported in humans and in many other animal species. In 1952, Prototheca was first linked to bovine mastitis by Lerche, and since then there were several studies about outbreaks of bovine protothecal mastitis in many countries such as German, America, China and Japan in the past twenty years. No wonder it has gradually attracted attention ...

Po-Ching Song, 宋柏青

博士 / 國立中山大學 / 海洋科學系研究所 / 104 / Coral bleaching is the consequence of disruption of the mutualistic Cnidaria-dinoflagellate association. Elevated seawater temperatures have been proposed as the most likely cause of coral bleaching whose severity is enhanced by a limitation in the bioavailability of iron. Iron is required by numerous organisms including the zooxanthellae residing inside the symbiosome of cnidarian cells. However, the knowledge of how symbiotic zooxanthellae obtain iron from the ho...

KUAN, AI-WEI, 官艾葳

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 104

Chung, Shun-Min, 鍾順民

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 104

Yi-Siou Li, 李易修

碩士 / 康寧大學 / 休閒資源暨綠色產業研究所 / 101 / This study aimed to build a microbial fuel cell (MFC) system and to perform tests in three sets of environments with different cathode chambers and anode chambers: (1) the anode chamber contained strict anaerobes and the cathode chamber contained Nannochloropsis oculate; (2) the anode chamber contained Aurantiochytrium sp. and the cathode chamber contained buffer solution; and (3) the anode chamber contained Aurantiochytrium sp. and the cathode chamber contained ...

Shi-Hua Gi, 紀熙華

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 93 / Pentavalence arsenic and trivalence arsenic chemical compounds are toxicant in the environment and methylation of arsenic species is a detoxification pathway in some organisms. Experimental analysis by HG-GCT-AAS(Hydride Generation-Gamma Cold Trap-Atomic Absorption Spectrophotometer)showed that marine alga Tetraselmis chuii cultrued with 0.133mM arsenate for 7 days generated monomethyl arsenic species (MMA) and dimethyl arsenic species (DMA), but freshwater alga Ch...