Chu-Ying Cheng, 鄭竹螢

碩士 / 國立中興大學 / 食品暨應用生物科技學系所 / 100 / Glutaredoxins (Grxs) are ubiquitous oxidoreductases in aerobic organism. They catalyze glutathione-dependent reactions to reduce protein disulfide linkage caused by oxidative stress. A full length cDNA encoding a glutaredoxin gene with 321 nucleotides was isolated from Chlorella sorokiniana T89. Nucleotides sequence analysis showed that it deduced 106 amino acid residues, the molecular weight of protein is predicted approximately 11.6 kDa. The deduced amino...

Chen Hsi, 陳曦

碩士 / 國立雲林科技大學 / 環境與安全衛生工程系 / 105 / Microalgae biofuels is currently still facing technical and economic difficulties on commercial roads, where the harvest cost only accounts for 20% of the total cost of technology. The two major reasons to explain why harvest cost is lower than expected is because the diameter of microalgae is extremely small with a typical length of 3 to 30 microns which makes collection is increasingly more difficult, and the fact that the surface potential of micro algae is ...

Ho, Tsung-Lin, 何宗霖

碩士 / 國立暨南國際大學 / 土木工程學系 / 105 / Diatomaceous earth is used extensively in concrete and mortar for weight- reduction and heat insulation. With the focus on reducing thermal conductivity and maintaining sufficient compressive strength, this study examined the effect of diatomaceous earth on the physical- mechanical properties and thermal conductivity of clay-based ceramics. Two clay raw materials were used for comparison. The microstructure and physical-mechanical properties of fired samples were e...

Jin-Mien Chiu, 邱靖棉

碩士 / 國立中興大學 / 基因體暨生物資訊學研究所 / 99 / The heat-talent Chlorella sorokiniana T89 was cloned from Chiayi paddy field. The genomic DNA and cDNA annotated data was unpublished so far. The lipid content of C. sorokiniana T89 was up to 30% and rich in unsaturated fatty acids which were suitable as biodiesel and functional foods. Culturs of C. sorokiniana T89 at different growth stages were used for RNA extracted and converted into cDNA for future sequencing analysis. Then get the contigs sequence data by ...

CING-REN PENG, 彭慶仁

碩士 / 國立臺中教育大學 / 科學應用與推廣學系環境教育及管理碩士班 / 102 / Emissions form factory’s combustion fossil fuels are regarded as the main culprit of (CO2) emissions, Microalgae is shown good conversion efficiency for CO2 absorption, yet the problem is whether or not the chlorella vulgaris can survive in the environment of flue gas where it is constantly filled with high concentration CO2 and SOx with low pH. What are the optimal conditions for the growth of Chlorella vulgaris? In this study the Chlorella vulgaris...

Ya-Ting Chang, 張雅婷

碩士 / 國立臺灣科技大學 / 化學工程系 / 103 / Cyanophycin granule peptide (CGP) is a nonribosomally synthesized polypeptide. The polymer we used is composed of aspartic acid as the backbone, arginine and lysine as the side chain. Chondroitin sulfate, a kind of glycosaminoglycan abundant in the cartilage tissue of human, is a linear polysaccharide consisting of repeating disaccharide units of glucuronic acid and galactosamine, which is commonly sulfated at C-4 and/or C-6 of galactosamine. In this study, we fabri...

Chien-Che Hung, 洪健哲

碩士 / 國立臺灣科技大學 / 化學工程系 / 103 / Cyanophycin granule polypeptide (CGP) is a non-ribosomal protein synthesis, first found in 1887 BC when Borzi observed the Cyanobacteria under a microscope. Because the yield rate of CGP is too low by culturing Cyanobacteria, we implant the cyanophycin synthetase gene of Cyanobacteria into Escherichia coli (E. coli) and use Isopropyl β-D-1-thiogalactopyranoside (IPTG) or Lactose induction to increase the yield rate. Nature CGP consist of aspartic acid (Asp) and ...

Chia-Ling Huang, 黃嘉伶

碩士 / 國立臺灣科技大學 / 化學工程系 / 103 / Cyanophycin (CGP) and hyaluronic acid (HA) are capable of good biocompatibility and biodegradability, and there are pros and cons of both biomaterials. The structure of CGP is positive charged, favorable for cell-adhesion, under neutral environment, but the limitations of water solubility and mechanical strength result in the applications are yet widely available. HA is flexible, hydrophilic and similar to the structure of extracellular matrix, while the shortage of...

Lei Tang, 湯蕾

碩士 / 國立中央大學 / 環境工程研究所 / 104 / The purpose of this study was to investigate the nitrogen and phosphorus removal efficiency from wastewater by immobilized microalgae, which mainly used to overcome the problems of collecting and separating Chlorella sp. after wastewater treatment. The experiments were carried out with synthetic as well as real wastewater in batch modes under different operation parameters. The results of synthetic wastewater tests indicated that the maximum 70.5% of ammonia remova...

Yen, Shao-Chieh, 顏紹傑

碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 103 / Genome editing is considered as an important technology to study a gene’s function. Among genome editing tools, recently developed CRISPR/Cas9 system is considered the most powerful one, since a single-stranded guide RNA (sgRNA) and one nuclease (Cas9) are the only two components needed to make a double strand break (DSB) in a specific site on chromosomes. As a consequence, DSB may induce nonhomologous end-joining (NHEJ) or homology-directed repair (HDR) that ...

Ting-Wen Chen, 陳亭妏

碩士 / 國立中興大學 / 食品暨應用生物科技學系所 / 101 / The gene of glutaredoxin (Grx) from Chlorella sorokiniana T89 (CsT89) has been cloned and overexpressed successfully in E. coli. The recombinant protein was biochemically characterized. In order to realize the structural and functional relation, in this study, based on the glutathione (GSH) binding sites and conserved residues of glutaredoxin, 12 single point mutations were designed from CsT89 Grx. Twelve mutations were designed as the following rCsT89 GrxK22R,...

Yu-Chien Kuo, 郭育謙

碩士 / 國立中興大學 / 生物產業機電工程學系所 / 99 / The main propose of this study was to establish a rapid and accurate indicator of microalgae growth parameters such as microalgae cell size, cell division rate and absorption spectra. The cell size and cell division rate of five species (Chlorella sp., Nannochloropsis oculata, Isochrysis aff. galbana, Pavlova lutheri and Botrycocccus braunii ) were studied to correspond with microalgae growth phase and cell density, and to determine the feasibility of them as the...

Jia-Hui Lee, 李加惠

碩士 / 國立中興大學 / 基因體暨生物資訊學研究所 / 99 / A cDNA encoding a cinnamyl alcohol dehydrogenase (CAD) gene with 1155 mucleotides was isolated from Chlorella sorokiniana T89. Recombinant CAD protein was expressed in Escherichia coli as a C-terminal histidin-tagged fusion protein and purified by DEAE-Sephacel ion exchange chromatography and TALON affinity chromatography. By these steps, the CAD was purified 1250 fold over the crude protein, and the total recovery was 3%. The optimal pH for the recombinant CAD ...

Meng-Che Li, 李孟哲

碩士 / 國立臺灣科技大學 / 化學工程系 / 104 / Cyanophycin is a non-ribosomal polypeptide that can be found in most cyanobacteria and some heterotrophic bacteria. The structure of natural cyanophycin lacks lysine, and after the genetic recombination, it contains aspartic acid as the main chain, and arginine and lysine as the side chain. The primary amine on lysine makes it easy for chemical modification. This study tried to graft water-insoluble cyanophycin with different oligosaccharides and al...

Chien-Yu Chen, 陳建宇

碩士 / 國立臺灣科技大學 / 化學工程系 / 104 / Thermo-responsive polymer is one kind of smart materials. The changes in temperature can alter the hydrophilic−hydrophobic surface conduct to induce the phase change, and thereby alter polymer shapes. The applications of thermo-responsive polymers have been proposed and demonstrated in different fields, such as temperature-triggered drug release, gene delivery, tissue engineering and bio-separation. In this study we used maltose, maltotriose and maltodextrin to modi...

LAN, CHI-CHANG, 籃啟昌

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 104

Hung-Yu Chiang, 江宏宇

碩士 / 國立臺灣大學 / 生化科學研究所 / 104 / Fucosylation is one of the most common modifications of oligosaccharides on glycoproteins and glycolipids. These fucosylated glycans usually function as special epitopes that contribute to specific physiological effects, including host-microbe interactions. Bacteroides fragilis is known to be one of the most common commensal bacteria in the human gastrointestinal tract. To avoid the host’s immune system, B. fragilis uses salvage pathways to utilize fucose from the h...

Chun-Hsiao Liu, 劉俊孝

碩士 / 國立中興大學 / 生物產業機電工程學系所 / 102 / The effects of microalgae post-harvest and its drying models were investigated. In this work, an airlift photobioreactor system for microalgae culture, equipped with a 100 liters tank, a temperature-reducing device, an airlift circulation device, and a movable illumination device, was designed and built. The efficiency of temperature-reducing, airlift circulation, and microalgae culture was tested respectively. The results showed that the temperature-reducing de...

Yu-Hui Chang, 張玉慧

碩士 / 國立臺灣大學 / 臺大-復旦EMBA境外專班 / 104 / Recently, customer are getting concern about the indoor air-pollution issues. The up-to-date reports indicate the majors causes are: 1. Excess or improper decoration which yields VOC and toxic substances. 2. Highly humid environment facilitate the reproduction of dust mite and mold which trigger allergic symptom, such as asthma, dermatosis, etc. Therefore, the cleanness and humidity-control of indoor air are crucial to health. The diatomaceous building materia...

LEE,CHIA-HSIN, 李嘉欣

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 105

Yi-Chien Chen, 陳怡倩

碩士 / 國立臺灣大學 / 漁業科學研究所 / 92 / Abstract Prorocentrum lima and three new species of Prorocentrum were identified according to their external features of cells, such as, shape and size, and ultrastructures of thecal plate ornamentation, intercalary band and architecture of the periflagellar area and grouped into three categoryies. Specimens identified in this research were previousely cloned from samples collected from the coastal area of Taiwan. Idnetification of these pecimens was further support...

Pei-Chi Tu, 涂珮琪

碩士 / 國立臺灣大學 / 植物學研究所 / 91 / Water bloom resulted from Peridinium bipes, a freshwater dinoflagellate, has commonly occurred in Dergie dam of the mid of Taiwan. Allelopathic activity was found in the water-soluble extracts of this alga. This study is aimed to study the toxic effects and to characterize the allelochemicals. Seven of thirteen strains of phytoplankton tested were susceptible to the water-soluble extracts of P. bipes. A further study done with Microcystis aeruginosa showed that the ph...

Ying-Hao Hung, 洪英豪

碩士 / 國立臺灣海洋大學 / 食品科學系 / 93 / Abstract The polysaccharide extracts of Grateloupia (G.) filicina which was purchased from the market were digested by four enzymes, α-amylase, cellulase, β-galactosidase, and agarase that produced from Pseudomonas vesicularis MA103. From the four hydrolysates, two enzymes were selected by higher reducing sugar contents, and the polysaccharide extracts of G. filicina were digested by the two enzymes. The antioxidation of lactic acid fermented the hydrolysates was ...

Chun-Liang Wu, 吳淳亮

碩士 / 國立臺灣海洋大學 / 食品科學系 / 93 / The purpose of this thesis was conferring the effect of extraction conditions on the antioxidative activity of Grateloupia filicina. After homogenized and freeze-dried G. filicina into powder, separately extracted of methanol, ethyl acetate, water, 90% methanol with water, 90% methanol with 0.01M hydrochloric acid, and made the extractions into dry powder, then compared their antioxidative activities on DPPH scavenging, reducing power, ferrous ion chelating, inhibiti...

Tzu-Chien Peng, 彭子倩

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / Astract Spirulina platensis, the blue-green microalga, has been the materials for commercial high-quality health food, due to it has high content of proteins as well as minerals, vitamins and fatty acids, etc. Beside this, its polysaccharide could inhibit metastasis and invasion of tumor, also inhibit the replication of some enveloped virus. Therefore, it has high values of medical therapeutics. The effects of varied light intensities, temperatures and salinities o...

Chung-Wei Lai, 賴仲威

碩士 / 中原大學 / 生物環境工程研究所 / 101 / To develop biomass energy is getting serious because of shortage of fossil fuel and low polluting production such as sulfites and carbon dioxide especially treating microalgae as a material. The microalgae are capable to transform the inorganic and organic carbon into lipids via photosynthesis which compositions are not only similar to diesel but also produce less pollution. In this study, we had selected and purified the superior algae to construct our experimental...

Yi-Hsin Tsai, 蔡宜欣

碩士 / 國立體育大學 / 運動科學研究所 / 103 / Purpose: Laminaria Japonica Fucoidan (FD) is a well-known bioactive constituent of seaweed extract which possess a wide spectrum of activities in biological systems including anti-cancer, anti-inflammation and modulation of immune systems. However, there are few studies for the use of FD as supplement for exercise performance and physical fatigue. We aimed to evaluate the potential beneficial effects of FD on ergogenic and anti-fatigue functions following physiologi...

Ying-Siou Chen, 陳盈秀

碩士 / 國立彰化師範大學 / 生物學系 / 102 / Chlorella pyrenoidosa is an economic alga and has been defined as a green healthy food by Food and Agriculture Organization of the United Nations (FAO). It is also widely applied as an important ingredient for food industry. Given the same organic cultivation is a growing trend in recent years. Creating a safe, high-functional nutrition, and rich flavor agricultural products, which can be safe to eat and cost-effective for the life is not the only one thing. Creating...

Syuan-Jyun Lin, 林炫君

碩士 / 淡江大學 / 化學工程與材料工程學系碩士班 / 100 / Dynamic microfiltration with a rotating-disk is used for the separation of microalgae from harvest suspension in this study. The effects of operating conditions, such as disk rotating speed, suspension feed rate, transmembrane pressure on the filtration rate, membrane fouling, cake properties and microalgae rejection, are discussed both experimentally and theoretically. Since the shear stress acting on the membrane surface may be increased by increasing the dis...

Hung-Cheng Lin, 林鴻成

碩士 / 崑山科技大學 / 環境工程研究所 / 100 / The species of Cladophora (Cladophoraceae: Cladophorales: Ulvophyceae: Chlorophyta) are worldwide distributed. They present in sea water, brackish water and fresh water. There are more than 400 species in the world, mostly present in sea water. In Taiwan, 13 species have been published so far, and all of them present in sea water. The yearly fresh water quality of Taiwan is rather hard (The main fresh water area lies between the south parts of Maoli to Hengchun Peni...

CHEN,DING-SIANG, 陳鼎翔

碩士 / 大葉大學 / 環境工程學系碩士班 / 102 / Effects of sodium bicarbonate and sodium nitrate concentrations on the growth rate of Nannochloropsis sp. and lipid content were investigated under different dilution rate using nitrate as the nitrogen source in this study. The main results are as follows. (一) Volumetric productivity of biomass The highest volumetric productivity of 0.31 g/L/day was obtained with the feed concentration of 0.1 g/L sodium nitrace under the dilution rate of 0.04 1/hr in the continuous ...

Ming-Jhan Hu, 許銘展

碩士 / 大葉大學 / 生物產業科技學系 / 101

LO,CHANG-YU, 羅章鈺

碩士 / 大葉大學 / 環境工程學系碩士班 / 101 / Sodium bicarbonate was used as the carbon source for batch cultures of Nannochloropsis sp. in this study. Factors investigated for growth and lipid accumulation of Nannochloropsis sp. were culture pH, concentrations of sodium bicarbonate and sodium nitrate. Higher biomass concentrations, biomass productivity and lipid content were obtained from cultures without the pH control. The highest biomass concentration was 0.8 g/L obtained in culture with the controlled cond...

Chi-ChiaLin, 林琪家

碩士 / 國立成功大學 / 地球科學系碩博士班 / 100 / The calcium oxide is a potential CO2 sorbent due to its great CO2 adsorption capacity. However, the surface area and porosity of CaO are eliminated by the sintering effect during the sorption/desorption process, then the adsorption capacity and the stability would be declined. To overcome these drawbacks, CaO is combined with the diatomite to cease the side-effect of sintering. Diatomite is a porous material with high specific surface area, surface electronegativi...

Jung-CheKuo, 郭榮哲

碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 100 / In the laboratory previously research, we have found that applied a marine microalga Picochlorum sp. S1b in tanks of grouper (Epinephelus coioides) larvae which significantly reduce the number of vibrio density in the water. We speculated that S1b produce vibrio static ingredients, compete-specific nutrition or some bacteria symbiosis with S1b inhibit vibrio growth. We selected two species of vibrio, Vibrio harveyi strain BCRC12907 and V. campbellii strain BCRC1...

Bo-Yu Lin, 林柏諭

碩士 / 國立臺灣海洋大學 / 輪機工程系 / 99 / Supersound and microwave were used to assist the lipid extraction of microalgae by solvents in this study. Crude lipid extracted quantity between the supersound and microwave-assisted lipid extraction under various solvents, extracting time and power were compared. The effect of emulsified solvent on the crude lipid extraction ratio was studied as well. The experimental results show that Isochrysis galbana extracted by microwave using the solvent mixture of n-heptane...

林睫侖

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / This study investigated the hot water extration of Chlorella pyrenoidosa (Chlorella pyrenoidosa extract, CpE) for inhibiting influenza A virus infection on in vitro and in vivo model. In vitro cell aaay, the sequence of CpE treatment is according to the adsorption step of virus infection; then divide into before virus adsorption (pre-treatment) and after virus adsorption (post-treatment). We use cell viability assay (MTS assay), cytopathic effect (CPE) assay, plaque ...

Wen-Chien Yen, 顏妏倩

碩士 / 國立臺灣大學 / 法醫學研究所 / 99 /

Yung-Cheng Shin, 辛永誠

碩士 / 國立臺灣大學 / 生化科技學系 / 99 / SUMO (small ubiquitin-like modifier) was originally discovered in 1996, as a peptide associating with RanGAP1 (Ran GTPase-activating protein 1). More studies subsequently found that SUMO can modify many proteins and influence many cellular processes of gene expression and signal transduction in eukaryotes. However, investigation of sumoylation on plant systems, especially on unicellular green algae, remains little. Thus, this work was aimed to study the SUMO system in...

Chih-Chung Chou, 周至中

碩士 / 國立臺灣大學 / 環境工程學研究所 / 99 / The reason for the dominance of Microcystis in stratified water bodies found in previous studies is not clear. It is still difficult to predict when Microcystis will dominate or to provide a good preventive strategy without the understanding of the controlling factors of the growth of Microcystis. The purposes of study include establishing the methods for analysising the density of algal cells, better understanding the buoyant mechanism of Microcystis and establishi...

Tian-Jiun Yu, 余天峻

碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / The freshwater cyanobacterium Microcystis aeruginosa has been extensively studied. The use of strain M‧TY-1in our laboratory is to produce MC-LR. However, the fractions without MC-LR was not studied. It is known that Microcystis aeruginosa produces many classes of natural products. Several hypotheses on the function of these compounds in the physiology and ecology of cyanobacteria have been discussed, mostly related either to grazing protection or to allelopathy. He...

Chun-HanChen, 陳君函

碩士 / 國立成功大學 / 熱帶植物科學研究所 / 101 / Regulation of the cell cycle is essential to drive cell proliferation. Chlamydomonas reinhardtii is a unicellular green alga that utilizes a specialized cell cycle program, multiple-fission, for cell division. In this study, smt15-1 mutant was isolated as a cell size mutant that had defective putative sulfate transporter and aberrant cell cycle program. smt15-1 had increased amount of total glutathione and failed to fully acclimate to sulfur starvation condition. ...

Yi-HsiangHsu, 徐溢祥

碩士 / 國立成功大學 / 化學工程學系碩博士班 / 101 / This study presents the effects of alternating frequency and duration of electric field on the fluorescence labeling of Chlorella vulgaris by two lipid probes: Nile red and Lipid TOX® Green. The application of electroporation on releasing cellular contents is also investigated as well as the feasibility of this technique in quantifying cell compositions. Conventional gravimetric methods for quantifying lipid amounts inside microalgae are complicated and time cons...

Shang-Rong Wu, 吳尚容

碩士 / 國立臺灣大學 / 生物環境系統工程學研究所 / 98 / Micro-algae have long been important ingredients of health food. In addition, considerable amount of lipid in their cells is found to be a promising source of bio-diesel fuel, therefore ever-increasing budget has been put into micro-algae culture industry in recent years. Nevertheless, given that recording and monitoring concentration of suspending cells remain tasks performed manually, both laborious and time-consuming, the ideal goal of automated micro-algae ...

Kun-Nan Tsai, 蔡昆男

碩士 / 長庚大學 / 基礎醫學研究所 / 89 / A large outbreak of enterovirus 71 occurred in Taiwan in 1998. More than hundred thousands of people got infected, among these with approximately 80 died. Symptoms at early stage include common cold、hand-foot-and-mouth disease ( HFMD ) and herpangna. Complication can be fatal as meningitis,myocarditis, poliomyelitis and flaccid paralysis. To advance the anti-enterovirus drug development is, therefore , important to this matter. Various kinds of phycobiliprotein were fo...

林育德

碩士 / 國立海洋大學 / 食品科學系 / 90 / The aims of this study are to explore the effects of molecular weight, degrees of deaectylation, concentrations of chitosan and gelling pH on physical-chemical properties of complex hydroges prepared from chitosan and alginate. Physical-chemical properties includes dynamic viscoelastic, water absorptivity and thermal properties. Chitin was extracted from red shrimp (Solemocera prominenitis) process waste. Three different degrees of deacetylation chitosans were prepared...

Chen Meiching, 陳美卿

碩士 / 國立中興大學 / 生命科學系 / 91 / In this study there are three sampling field located on each estuary of Ta-Chia-Shi, Ta-Du-Shi, and Chuo-shue-Shi. The epipelic and episamic diatoms were collected seasonally from autumn 2001 to winter 2003. A total of 159 diatom species were observed, and 140 species were indentified. Pennate diatoms were generally the most prevalent of them. The diatom communities of each sampling field could be clustered to three groups by using the software Primer 5.0. The dominant...

Kuo-Wei Chang, 章國威

博士 / 國立中興大學 / 生命科學系 / 91 / Abstract The microscopic planktonic algae of the world’s oceans are critical food for filter-feeding bivalve shellfish such as, oyster, mussels, scallops and clams as well as the larvae of commercially important crustaceans and finfishes. Some microscopic planktonic algae have the capacity to produce potent toxins that may be transfered through fish and shellfish to humans. On a global base, near 2000 cases of human food poisonings (15% mortality) from consu...

王藝蓉

碩士 / 大葉大學 / 食品工程學系碩士班 / 91 / Microalgae is able to accommodate the natural environment well and can grow almost everywhere. Production of lipid through a culture of microalgae is superior to that from the cultivation of animals and plants, because microalgae can grow almost without the limitation of time or space, and can produce the lipids which are necessary to human being. In terms of the growth rate, the microalgae is not as good as fungus, but the cultivating condition for microalgae is m...

李錦堃

碩士 / 東吳大學 / 微生物研究所 / 75 /