Hou-Sheng, Lin, 林火盛

碩士 / 國立宜蘭大學 / 綠色科技學程碩士在職專班 / 101 / Recent ten years, massive soil outflow on upstream of Shimen reservoir, increased sedimentation seriously year by year, reduces service life of the reservoir, and the ability of the water supply and flooding prevention. If we can add the wastes in the reservoir silt, prepared the lightweight aggregate that made resources and reutilization effectively, the product would produce building materials of lightweight function of sound absorption, heat insulation, vibr...

Jun Yi Lee, 李俊毅

碩士 / 大葉大學 / 環境工程學系碩士班 / 101 / In this study, light indensity distribution in a cylindrical photobioreactor was examined for cultures of Tetraselmis Chui by measuring light intensity in situ in the photobioreactor. Simulation for effects of light intensity, biomass concentration and radias of cylindrical photobioreactor on growth were investigated using a Haldane growth model for cultures of Tetraselmis Chui. Major events as following： （1）Biomass concentration and radius of a cylindrical photobi...

Jhin-heng Chuen, 闕志衡

碩士 / 大葉大學 / 環境工程學系碩士班 / 101 / In this study effects of culture conditions on growth of Nannochloropsis sp. were examined using response surface methodology and a mathematical modal was established to find the optimal condition for biomass production. The appropriate range of each factor was determined for growth with the method of one-factor-at-a-time. Then central composite design was set up for factors of sodium bicarbonate concentration,salt concentration and pH based on the results obtained....

Liu, Yun Chi, 劉芸綺

碩士 / 明志科技大學 / 生化工程研究所 / 100 / In order to develop the microalgae strains which produce highly efficient and critical enzyme during lipid synthesis and metabolism, this study utilizes the genetic engineering approach to designs a novel expression vector for higher efficiency of oil production in Microalgae. in this research, we develop a series of expression vectors, four promoters (PCaMV35s, PRBCs, PAMT, PNR) were selected for driving the foreign genes (GPAT, LPAAT, PAP, DGAT) from Chlamydomonas...

Chu-Sian Jhu, 朱俶嫻

碩士 / 國立東華大學 / 海洋生物科技研究所 / 99 / In the present study, we investigated the correlation between lipid components and cell proliferation among differernt clades (Clade B1, E1 and C1) of Symbiodinium. The clades B1, E1 and C1 of Symbiodinium were cultivated for three days under 12L/12D photoperiod and cell proliferation was analzyed using flow-cytometer. The cell cycle propogation of the Symbiodinium clade B1 and E1 can be synchronized under 12L/12D photoperiod. 40~60％ of clade B1 underwent cell prol...

Wei-Cheng Lin, 林威成

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / Gymnodinium catenatum is dinoflagellates formed chain-likes colony, which could produce paralytic shellfish poison (PSP). The aquatic animals which filter the foods will accumulate PSP in their bodies, and humans may be poisoned by the way of food chain. G. catenatum was discoveried in Maxico by Graham in 1943, and the food poisoning incidents appeared in Argentina, Uruguay, Australia, Morocco, Spain, Japan, and Philippines during recent twenty years. In recent years...

Wen-Hsien Hsu, 許文獻

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99

Chin-Nien Chuang, 莊謹年

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / Fucoidan is a complex sulfated polysaccharide with a wide variety of biological activities including anti-oxidant, anti-tumor, immunomodulatory, anti-complement, blood lipids reducing and anti-virus. The six hallmarks of cancer is grown signal autonomy, evasion of grown inhibitory signals, evasion of apoptosis, unlimited replicative potential, angiogenesis and metastasis. Malignant tumor is the leading cause of high death in the world. In fact, metastasis is the majo...

Ying-Chen Yang, 楊瀅臻

碩士 / 國立臺灣大學 / 環境工程學研究所 / 99 / In order to enhance the efficiency of anaerobic digestion, the effects of ozonation and high-pressure extraction were studied on microalgae. High-pressure extraction was performed with mildly elevated pressure in consecutive cycles of compression and decompression as pretreatment. Anaerobic digestion converts the organics present in microalgae into biogas, but the efficiency and the digestion rate are limited by the hydrolysis. Biodegradability of microalgae could b...

Pei-Chia Chuang, 莊珮嘉

碩士 / 國立臺灣大學 / 環境工程學研究所 / 99 / The algal bloom is a serious problem in reservoirs, not only would increase the cost of water treatment, but also result in various problems due to different algal species. Blue-green algae are often the dominant species. Microcystis is one of blue-green algae, and often dominates in reservoirs. We are also concerned about on Microcystis due to some species of Microcystis is able to produce toxins which pose serious health risks to human and animals. Microcystis is ...

Chuan-Ling Chou, 周傳鈴

碩士 / 國立臺灣大學 / 生態學與演化生物學研究所 / 101 / Diatom communities are often used as bio-indicators in reflecting water quality of the contemporary environment and for the reconstruction of the paleoenvironmental conditions. Also, the biogeography of diatoms has been studied and used in some applications. However, little is known about the biogeographic pattern of diatoms in Taiwan. The objectives of this study were to analyze the biogeographical pattern of diatom communities along the Tamsui River of northe...

Phoebe Beverly Chen, 陳品如

碩士 / 國立臺灣大學 / 食品科技研究所 / 101 / Neuroinflammation may play a role in the pathogenesis of Parkinson’s disease (PD), which is a common neurodegenerative movement disorder caused by the loss of nigral dopaminergic neurons. Major current treatment of PD is L-dopa, which can be the substitute of dopamine but unable to supress the neurodegeneration in the brain. Therefore, searching for components which can prevent the loss of dopaminergic neurons via regulating neuroninflammation may be a new strategy ...

Po-Hsun Lu, 陸博勛

碩士 / 義守大學 / 材料科學與工程學系碩士班 / 99 / Energy demand were increased with the improvement of quality of life, looked for the green energy was impartment agenda in the world. Several microorganisms such as green algae, cyanobateria heterocystous, cyanbacteria nonheterocystous and photo-synthetic bacteria were cultured to generate hydrogen gas. The advantages of the Chlamydomonas reinhardtii microbial fuel cell include simple structure, low cost, transformation of carbon dioxide into nutrients and unconfi...

Chih-Hsin Chen, 陳智信

碩士 / 義守大學 / 生物醫學工程學系碩士班 / 99 / A controlled pulsatile drug release platform combined with focused ultrasound and alginate microcapsules was established, and enhancement of anti-inflammation efficiency was achieved. An electrospray device (ESD) was employed to fabricate alginate microcapsules with good sphericity and uniform size. These microcapsules were used as carriers for diclofenac (75 % of encapsulation ratio), which was employed as a model drug to evaluate the efficiency of anti-inflammato...

Shih-Yuan Wang, 王識淵

碩士 / 輔英科技大學 / 生物科技系碩士班 / 99 / The objective of this study is to generate transgenic microalgae capable of producing phytase. Two acid phosphatase gene (PH01) expression vectors, pC35S-PH01driven by the 35S promoter and pHR-PH01 driven by Hsp70A-RbcS2 fusion promoters, were constructed and introduced into Chlorella sp. T-89 by electroporation. Stable transgenic lines were obtained by hygromycin selection. Successful transduction and integration of the transgene into host genomes were detected by...

Pao-Mei Yang, 楊寶美

碩士 / 國立中興大學 / 食品暨應用生物科技學系所 / 98 / Abstract Microalgae have drawn more and more attention as a promising source of biodiesel, so that rapid screening for algae strains with enhanced lipid production becomes essential. The main motivation of this study is to evaluate the feasibility of using flow cytometry for rapidly detecting the oil content in cultivated Chlamydomonas reinhardtii. Traditionally, the lipid content in microalgae samples was determined using extraction and weighing procedures, whi...

Ai-Ping Huang, 黃愛蘋

碩士 / 國立中興大學 / 環境工程學系所 / 98 / Microalga is a photosynthetic microorganism that uses the solar energy to combine water with CO2 create biomass. They can utilize NH4 +, NO3 -, and PO43- as growth nutrients, and remove these inorganic nutrients from wastewater. Microalgae have been suggested as very good candidates for fuel production. In this study the nitrogen and phosphorus assimilation and lipid production by microalgae using wastewater were studied in a 5 L photobioreactor in order to reach the...

Shu-Chiu Shie, 謝淑秋

碩士 / 國立中山大學 / 海洋生物研究所 / 99 / Chlamydomonas reinhardtii can utilize CO2 for autotrophic growth (HSM plus 5% CO2) or acetate for mixotrophic growth (TAP). This study was to elucidate the differential regulation of methionine sulfoxide reductase (MSR) gene expression between HSM plus 5% CO2 and TAP cultured cells, and also to determine the difference of gene expression in response to high light (1,000 μE m-2 s-1). The role of photosynthetic electron transport (PET) in the regulation of MSR gene exp...

Chuang, Meiyu, 莊梅鈺

碩士 / 靜宜大學 / 食品營養學系 / 100 / Leukemia is the twelfth of cancer causes of death in adult and the first cancer of death in children in Taiwan. Recently, there is an increasing interest in the study how to prevent or delay the leukemia development. Caulerpa microphysa (grape algae) belongs to the Chlorophyta division. In this study, we examined the antioxidant activities of Caulerpa microphysa pepsin-digested extract (CME) and its possible apoptosis mechanim in leukemia cells (WEHI-3 and HL-60). The ...

SU, JIA-LING, 蘇佳玲

碩士 / 國立中興大學 / 食品科技研究所 / 74 /

Huang, Sheng-Ping, 黃聲蘋

碩士 / 國立臺灣大學 / 海洋研究所 / 69 /

Teh-Yang Lin, 林德揚

碩士 / 國立陽明大學 / 醫學工程研究所 / 89 /

Shesu-Wen Tsai, 蔡曉雯

博士 / 國立陽明大學 / 醫學工程研究所 / 87 / The aim of this study is to prepare a collagen-based microcapsule for cell culture using alginate as an encapsulation medium. Furthermore, theoretical modeling has been conduct to simulate the concentration distribution of growth factor within the cell culture matrix. Collagen is the major structural protein in the extracellular matrices of connective tissues. With proper treatment, its immune response can be eliminated and provide a better biocompatibility. Purified...

Chien-Hsiung Hsu, 許建雄

碩士 / 大葉大學 / 環境工程學系碩士班 / 94 / The increase of the concentration of carbon dioxide in the atmosphere has been considered as the main cause of global warming. Fixation of CO2 using photosynthesis by microalgae is a kind of potential approach. The study aims to cultivate Tetraselmis Chui using bicarbonate ion as carbon source which is formed by alkaline absorption. Effects of culture conditions on the growth of Tetraselmis Chui were performed in batch cultures. The main results and conclusion as...

何詠寧

碩士 / 國立中正大學 / 分子生物研究所 / 90 / Abstract Protein phosphatase 1 (PP1) and 2A (PP2A) are the major eukaryotic serine/threonine protein phosphatases that play a critical role in regulation of diverse cellular functions. Both phosphatases are present in cell by association with the different regulatory subunits to form the various holoenzymes. Therefore, identification of PP1 or PP2A holoenzymes will extend our understanding of the important regulatory molecules that regulate cell signalin...

Shih-Yuan Chen, 陳士元

碩士 / 國立嘉義大學 / 水產生物學系研究所 / 94 / Abstract The aim of this study approaches the feasibility of using microalgae to uptake NH4+ in water. There are four parts in this study: the first is to approach the effect of temperature on growth rate and NH4+-uptake rate of four microalgae; the second is to approach the effect of culture media, algal growth stages and inoculated algal concentrations of algae on the growth of Tetraselmis chui; the third is to approach the effect of light-path length of algal i...

Yu-Jen Yeh, 葉郁仁

碩士 / 嘉南藥理科技大學 / 生物科技研究所 / 91 / In this study, a series of alginate/hydroxyapatite(HAP) composite scaffolds were prepared and characterized. HAP was incorporated into the alginate gel solution to improve the mechanical properties and biocompatibility of scaffolds. The scaffold was prepared by a three-step procedure: first gelation of the alginate solution to form a hydrogel with divalent cations (such as Ca2 ＋, Sr2＋and Ba2＋), then freezing, and finally drying by lyophilization to produce a three-...

Jieh-Ming Shieh, 謝介民

碩士 / 大葉大學 / 國際企業管理學系碩士在職專班 / 95 / About the research of the thorough channel structure, carry on the discussion to the division of labor angle between thorough channel members mainly, probe into the influence on the power, atmosphere, conflict and cooperation in the thorough channel of the structure of thorough channel, or probe into the relation on sale throughout the thorough channel and enterprise's whole performance. And the angle circulating with the thorough channel, it is less in differen...

Cheng-Hong Hsieh, 謝政宏

碩士 / 輔仁大學 / 生命科學系碩士班 / 95 / In this study, we intend to isolate microcystin from the mass culture of Microcystis aeruginosa. To establish the microcystis mass culture, we need to scale up the culture from the size of stock culture, 2L flask to 400L glass tank, using enriched media, irradiation and aeration. It was found the increase of culture vessel had lower stationary phase all density. We speculate it was due to the light limitation grow cell density and the length of light path. This is a n...

Jenn-Chyang Chen, 陳振強

碩士 / 國立中興大學 / 植物學系 / 81 /

Yi-Ling Wu, 吳宜玲

碩士 / 國立清華大學 / 分子與細胞生物研究所 / 92 / Helicobacter pylori is a Gram-negative microaerophilic bacterium and is an etiologic agent for gastric diseases such as gastric ulcer, gastric cancer, and duodenal ulcer. H. pylori was reported to form communities of surface-adherent organism embedded in an extracellular matrix, or biofilm that is water-insoluble and HCl-resistant. By scanning electron micrographs, our laboratory has observed how individual, spiral bacteria attached onto solid surfaces and progres...

Ying-Min Cheng, 鄭英敏

碩士 / 國立中山大學 / 海洋生物研究所 / 92 / Marine cnidarian-microalgal endosymbiosis is an ecologically important intracellular association. However, its underlying molecular mechanisms are essentially unknown. In light of the critical roles of host phagocytosis in intracellular fates of a variety of microbes, and the Rab small GTPases as key mediators of host-symbiont interaction, we set out to investigate the potential involvement of Aiptasia Rab proteins in the model photosynthetic endosymbiosis between th...

Cun-Da Tsai, 蔡坤達

碩士 / 中國文化大學 / 生物科技研究所 / 89 / A circadian oscillating protein of 23 kDa (COP23) located in the cell membrane of unicellular cyanobacterium Synechococcus RF-1 exhibits a diurnal rhythm after 12h L/12h D entrainment. The content of COP23 declined to nearly undetectable level at light phase and reached a peak during dark phase. If L/D-entrained culture of Synechococcus RF-1 was transferred to continuous darkness, the degradation of COP23 was prevented. However, if the dark culture was illuminated at...

Chan-Ting Yeh, 葉展廷

碩士 / 嘉南藥理科技大學 / 環境工程與科學系暨研究所 / 94 / This study focused on the isolation of attached diatoms taken from Szu-Tsao Wildlife Reserve and their tolerance of the heavy metals copper and cadmium. The results showed that the attached diatoms could be isolated from the nature and pure culture by the Marine enrichment medium f/2 medium under conditions of 30℃, pH8 and light intensity 100μmol．photons．m-2．S-1. Seven species were identified under the light microscope (LM) and scanning electric microscope(SEM...

Jui-Hsi Weng, 翁瑞禧

博士 / 國防醫學院 / 生命科學研究所 / 92 / Synechococcus sp. RF-1, a unicellular N2-fixing cyanobacterium, can grow photosynthetically and diazotrophically in continuous light. How the organism protects its nitrogenase from damage by oxygen is unclear. In this work, a PAM-101 fluorometer and an O2 electrode are used simultaneously to study the chlorophyll a fluorescence and to monitor O2 exchanges in Synechococcus sp. RF-1 cells. The results found that in addition to CO2, O2 can also act as electron acceptor t...

He-Chuan Sun, 孫鶴娟

碩士 / 中興大學 / 環境工程學系所 / 99 / Nowadays, oil and natural gas storage on earth has been estimated to be depleted in 50 and 64 years, respectively. The quick development of human activities cause aggravation of greenhouse effect by combustion of fossil fuel, coal, oil and nature gas has become a global issue. Biodiesel is a renewable resource of energy that could be sustainable supplied and no net increased release of carbon dioxide and very low sulfur content. It is biodegradable and nontoxic. Thus, ...

Po-shao Chen, 陳柏劭

碩士 / 國立臺灣科技大學 / 化學工程系 / 99 / Bacterial cellulose has recently attracted a great deal of attention for biomedical applications because of its good water retention, mechanical properties, and biocompatibility. However, the lack of suitable functional groups provides a poor surface for cell growth. Soluble cyanophycin is non-ribosomal protein which is composed of aspartic acid, arginine, and lysine. In order to improve cell adhension and growth on bacterial cellulose, we added water soluble cyanoph...

Chiung-yu Cho, 卓瓊玉

碩士 / 國立臺灣科技大學 / 化學工程系 / 99 / Cyanophycin granule polypeptide (CGP) is a non-ribosomal protein found in most groups of cyanobacteria. It was discovered in 1887 by Borzi during microscopic studies of cyanobacteria. Most cyanobacteria harbor a functional cyanophycin synthetase gene (cphA) and synthesize CGP. The branched polymer consists of an equimolar amount of arginine and aspartic acid. In this study, we used Synechocystis sp. PCC 6803 cphA in E. coli BL21-CodonPlus(DE3)-RIL to produce CGP. The...

Yin-Ta Chen, 陳映達

碩士 / 大仁科技大學 / 環境管理研究所 / 94 / Six green algae products and 6 blue-green algae products sold in Taiwanese markets were tested to determine the chemical and microbiological quality, antioxdant activity and antibacterial activity. It was found that the average values of proximate composition in green algae products and blue-green algae products were protein: 40.78 % and 57.36 %, lipid: 14.28 % and 16.57 %, ash: 7.79 % and 8.07 %, moisture: 6.98 % and 7.15 %, carbonhydrate: 30.17 % and 10.79 %, respe...

Chiu-Chu Hwang, 黃秋菊

碩士 / 大仁科技大學 / 環境管理研究所 / 92 / Paralytic shellfish poisoning (PSP) associated with food poisoning incidents have caused two human fatalities and many illnesses in Taiwan. The sourse of PSP in Taiwan was thought to be the toxic dinoflagellate Alexandrium minutum. To understand the trace elements in walne medium for cell growth and toxin production of A. minutum, the different concentration of copper and iron ion, and vitamin B group were added into the walne medium of A. minutum. It was found that ...

Tzu-Jung Wu, 吳紫容

碩士 / 嘉南藥理科技大學 / 化妝品科技研究所 / 98 / In this study,fermentation of the Chlorella and the plants by adding two kind of bacteria in culture medium and analyzed the functional effects of fermentative metabolites.These tests included the antibacterial, antioxidative and anti-aging effects. This experiment used the Bacillus subtilis to break the contents of Chlorella and the plants into small fragments then to ferment these samples by Saccharomyces cerevisiae. The resultants were extracted with ethanol an...

Shao-yu Su, 蘇紹宇

碩士 / 嘉南藥理科技大學 / 藥物科技研究所 / 98 / Wound pain, tissue trauma and infection are the main considerations of wound management, and appropriate dressing selection plays an important role in all. Traditional dressings may adhere to wounds resulting in significant pain and trauma to new tissue upon removal. Currently a lot of research is focused on developing newer dressing materials to treat wounds with coated medical devices to improve the functional and aesthetic outcome of the regenerating skin or sca...

Ying-ChiehChen, 陳英傑

碩士 / 國立成功大學 / 工程科學系碩博士班 / 99 / In this study, the polydimethylsilcoxane (PDMS) microfluidic chip is fabricated by using MEMS, casting molding, and microimprinting technology. The chip is used to study microcapsules of different concentrations. Our strategy is to simulate the velocity distribution of fluids in different microfluidic channels by using computational fluid dynamic (CFD) software. The results of simulation give us insight on how to design the wide of bifurcate channels and fabricate ...

Sun, Cheng-Hsiung, 孫證雄

碩士 / 東海大學 / 化學工程與材料工程學系 / 99 / In recent years, because the algae have a carbon reduction capacity, high lipid content and rich nutrient ingredients, it gradually attracted much attention. In this study, we explore three research directions by the cultivation of microalgae: (1) the effect of Scenedesmus obliquus concentration and lutein content by changing the environment or the medium composition. The results of this study indicate that an increase in CO2 percentage to 2% and light intensity to...

Yu-Ling Dong, 董祐伶

碩士 / 國立臺灣海洋大學 / 食品科學系 / 98 / Dengue virus (DV), mosquito-borne members of the family Flaviviridae, is the agent of the most prevalent viral disease in humans. Of the 1 million annual cases of dengue haemorrhagic fever/dengue shock syndrome, about 2 - 5% is fatal. Now, there is still no vaccine or antiviral drug to treat DV infections, although the sulfated polysaccharide from the algae is known to present anti-dengue viral activity. In this study, the antiviral activity of hot water extracts fro...

Ming-Chou Hsieh, 謝明洲

碩士 / 國立臺灣海洋大學 / 食品科學系 / 98 / Oxidative stress is now recognized as a common pathology that affects up to half of all infertile men. This study extracted powder of Ascophyllum nodosum by ethanol, acetone, supercritical fluid extract (SFE) and ethanol SFE respectively, and obtained ANE, ANA, ANS and ANC. ANA contained the most fucoxanthin content. And ANE contained the highest level of total polyphenolic content, also demonstrated the strong DPPH radical scavenging activity and reducing power. The...

Bi-Ru Lin, 林碧如

碩士 / 國立彰化師範大學 / 電機工程學系 / 100 / The study aims to improve and integrate the measuring methods of closed-path CO2 gas analyzer. A customized closed-path incubator with semicircle transparent acrylic is developed according to the environment to connect the gas with infrared CO2 gas analyzer by pump and gas circulating system at inlet and outlet. Through the non-dispersive infrared detecting technique, the analyzer can measure CO2 concentration and save related information between CO2 concentration ...

Li, Zhi-Xian, 李智賢

碩士 / 遠東科技大學 / 機械工程研究所 / 100 / This1study1investigated1the use1of brewery waste generated1by filtering as poregenerating additives, diatomaceous1earth, and the1 intelligent application1of the2previousresearch on water-saving environmentalplanting pots, andthen1to water1and1energy1conservation,1and1sustainable environmental1protection goals. The experimental1parameters is through1three clay substrate (white clay,1yellow clay, filling soil),1and add1a1different ratio (0wt.%,13wt.%,16wt.%,19wt.%,115...

Hsin-Yu Wang, 王馨玉

碩士 / 康寧大學 / 休閒資源暨綠色產業研究所 / 99 / Algae are not only nutritious, but also economically valuable. There is an abundance of algae in both sea waters and fresh waters. Currently algae are being developed mainly into bait, animal food and cosmetics. Algae with high lipids content are known as oleaginous algae. Recently there have been studies on using oleaginous algae as a source of biodiesel. This study aims to investigate the growth characteristics of oleaginous microalgae, including Nannochloropsis...

Ching-An Lai, 賴鏡安

碩士 / 元智大學 / 化學工程與材料科學學系 / 98 / Bio-energy and biodiesel has been approved to reduce the greenhouse effect and secure the energy supply. However the waste glycerol derived from biodiesel production became an environmental issue. In this study , an approach of using glycerol as the substrate to produce polyunsaturated fatty acid (PUFA) by microalgae was examined. Deoxyribonucleic acid (DHA) is a PUFA which can not synthesized by human but has been demonstrated helpful in preventing heart and circu...