陳胤維

碩士 / 國立彰化師範大學 / 台灣文學研究所 / 97 /

稲井, 啓之

Kyoto University (京都大学) / 0048 / 新制・論文博士 / 博士(地域研究) / 乙第13252号 / 論地博第24号 / 新制||地||94(附属図書館) / 京都大学大学院京都大学大学院アフリカ地域研究専攻 / (主査)教授 木村 大治, 准教授 大山 修一, 准教授 安岡 宏和, 教授 市川 光雄 / 学位規則第4条第2項該当

Tsai,Wei-Shiun, 蔡偉勳

碩士 / 國立彰化師範大學 / 生物學系 / 101 / Crustacean hyperglycemic hormone (CHH) was originally identified in a neuroendocrine system－the X-organ/sinus gland complex. In this study, a cDNA(Prc-CHH) encoding CHH precursor was cloned from the hemocyte and hematopoietic cells (HpT cells) of the crayfish Procambarus clarkii. Analysis of tissues by a CHH-specific enzyme-linked immunosorbent assay (ELISA)confirmed the presence of CHH in hemocytes and HpT, the levels of which were much lower than those in the sinus...

Chen, Yun, 陳昀

碩士 / 國立彰化師範大學 / 生物學系 / 106

Lin Line-Jiun, 林玲君

碩士 / 國立彰化師範大學 / 生物學系 / 98 / Six transcripts, each encoding a distinct CHH (crustacean hyperglycemic hormone) or CHH-like (CHH-L) peptides, Procambarus clarkii - CHH1, CHH1-L, truncated-CHH1 (t-CHH1), CHH2, CHH2-L, and t-CHH2, were identified in the crayfish. These transcripts were generated by alternative splicing of RNA precursors of two CHH genes (chh1 and chh2). The present study examined the effects of white spot syndrome virus (WSSV) on the CHH and CHH-like peptide system. Hemolymph CHH1...

Shao-Chen Chang, 張韶真

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 96 / A survey was conducted to examine the diversity, and temporal and spatial variation in the decapod (crabs) community structure in sandy shore, boulder shore, and estuary habitats in Homei, Taipei County, northeastern Taiwan from January 2007 to December 2007. A total of 21 species were collected in the estuary, including 11 grapsid species, 3 eriphiid species, 1 porcellanid species, and 4 species of hermit crab. The shore crab, Ptychognathus barbatus, was the m...

Shao-Yen Huang, 黃劭彥

碩士 / 國立彰化師範大學 / 生物學系 / 100 / In the purpose, analysis of 2 mGC genes, PcGC-M2 and PcGC-M2B, were cloned from the muscle of the crayfish in our previous studies and evaluated for their binding affinities of polymorphic CHH peptides to PcGC-M2 and PcGC-M2B are compared. The 2 mGC proteins are identical in containing the same extracellular binding and transmembrane domains, while PcGC-M2 also contains a kinase homology domain and a cyclase catalytic domain and PcGC-M2B only a truncated kinase homol...

Kuo-Wei Tsai, 蔡國瑋

碩士 / 國立彰化師範大學 / 生物學系 / 93 / Crustacean hyperglycemic hormone (CHH) is a neuropeptide original identified from the X-organ (XO)-sinus gland (SG) complex in the crustacean eyestalk. In the present work, three full-length CHH cDNAs were cloned from the mud crab Scylla olivacea eyestalk ganglia (ES), thoracic ganglia (TG), and per- icardial organ (PO) by RT-PCR and rapid amplification of cDNA end (RACE). PO-CHH and TG-CHH have the same deduced amino acid sequence. TG/PO-CHH and sinus gland CHH (SG-C...

蔡士甫

碩士 / 國立彰化師範大學 / 生物學系 / 104 / Crustacean hyperglycemic hormone (CHH) is the hormone which is produced and secreted by eyestalk ganglion of crustaceans. The most important physiological function of CHH is influencing the glycogen metabolism in the target tissues (muscle, liver, pancreas and other tissues) and promoting the glucose mobilization. It may affect crustacean immune function indirectly. The previous study showed that the CHH could promote the glycolysis and the pentose phosphate pathway ...

Tien Yi-Chun, 田逸群

碩士 / 國立彰化師範大學 / 生物學系 / 102 / CHH (crustacean hyperglycemic hormone) was originally identified in a crustacean neurosecretory complex, the X-organ/sinus gland. Previous studies, CHH might directly or indirectly regulate carbohydrate metabolism, thereby affecting the immune system of crustaceans. Therefore, this study first established RNA interference on Procambarus clarkii, and confirmed CHH gene silencing by real-time quantitative polymerase chain reaction. It might cause physiological changes ...

宮珮真

碩士 / 國立彰化師範大學 / 生物學系 / 97 / Crustacean hyperglycemic hormone (Crustacean hyperglycemic hormone1, CHH1) is a neurohormone originally identified in the X-organ sinus gland (XO-SG) of the eyestalk ganglia. According to the combined data from our previous and this studies, at least six CHH transcripts were present in the crayfish (Procambarus clarkii) : CHH1, CHH1-L (CHH1- like), t-CHH1 (truncated-CHH1), CHH2, CHH2-L (CHH2- like), t-CHH2 (truncated-CHH2). I studied the structural organization of CHH...

Hsin-Ju Wu, 吳欣儒

碩士 / 國立彰化師範大學 / 生物學系 / 97 / CHH (crustacean hyperglycemic hormone) is a neurohormone produced and secreted in the X-organ/sinus gland complex (XO/SG) of the eyestalk ganglia in crustacean. CHHs process molecular polymorphism, multiple functions and express in various tissues. Six CHHs mRNA (CHH1、CHH1-L、t-CHH1、CHH2、CHH2-L and t-CHH2) were discovered in the crayfish Procambarus clarkii (Yasuda et al., 1994；Kupper, 2002；Wu, 2005；Kung, 2009). In this study, western blot analyses indicated L/D-phe3 C...

Chih-Chun Chang, 張智鈞

碩士 / 國立彰化師範大學 / 生物學系 / 96 / Sco-CHH and Sco-CHH-L (CHH-like peptide), two structural variants of the crustacean hyperglycemic hormone family identified in the mud crab (Scylla olivacea), are identical up to the 40th residue, but different from each other in the remaining sequence. In this study, recombinant proteins (rSco-CHH and rSco-CHH-L) were produced by an E. coil expression system, refolded, purified, and confirmed by Western blotting and mass spectrometric analyses. Purified rSco-CHH was ...

Jia-Ying Jiang, 江佳穎

碩士 / 國立彰化師範大學 / 生物學系 / 96 / Over the years, researchers have found there are different CHH (crustacean hyperglycemic hormone) molecules in crustaceans. According to those studies there were six CHH/CHH-like peptides in crayfish (Procambarus clarkii ) ：CHH1、CHH1-L、CHH2、CHH2-L、Hem-CHH1 and Hem-CHH2. Methods of molecular cloning resulted in full length sequence of hemocyte CHHs (involve untranslated region, UTR). Moreover with RT-PCR analysis, these CHH/CHH-like peptide genes were widely expressed ...

Chi-Chih Juan, 阮季芷

碩士 / 國立彰化師範大學 / 科學教育研究所 / 89 /

Ting-Yu Chen, 陳亭宇

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 101 / The neuropeptide F (NPF) are proposed by some to be the homologs of vertebrate neuropeptide Y. Feeding regulate on genes are widely distributed in the central and peripheral nervous system.The neuropeptide F (NPF) could mainly regulate food intake, metabolism, reproduction, and stress reaction in invertebrate. Our experiment research discussed the expression of feeding regulator-Neuropeptide F gene on Litopenaeus vannamei and Eriocheir sinensis feeding behaviors. T...

Su-Hua Wu, 吳素華

碩士 / 國立彰化師範大學 / 生物學系 / 93 / Crustacean hyperglycemic hormone (CHH) is a neurohormone originally identified in the X-organ sinus gland (XO-SG) of the eyestalk ganglia and primarily involved in the regulation of carbohydrate metabolism. Sequence analysis of CHHs isolated from various decapod crustaceans shows that they are peptides of 72–73 amino acid residues. The present study presents data indicating that CHH-like molecules are expressed in the hemocytes of the crayfish Procambarus clarkii. Sig...

Hong-Shin Zou, 鄒浤鑫

碩士 / 國立彰化師範大學 / 生物學系 / 89 /

陳彥州

碩士 / 國立彰化師範大學 / 生物學系 / 100 / CHH (crustacean hyperglycemic hormone) was originally identified in a crustacean neurosecretory complex, the X-organ/sinus gland. In addition, we purified the native CHH1 and CHH1-L (CHH1-like) from neurosecretory tissues. Tissue extracts (sinus gland: SG; thoracic ganglia: TG) were chromatographically separated. CHH1 and CHH1-L, two structural variants of the crustacean hyperglycemic hormone family identified in the crayfish (Procambarus clarkii), and assays of biol...

吳佳倫

碩士 / 國立彰化師範大學 / 生物學系 / 92 / Abstract Nitric oxide (NO) is an important intercellular molecule.. NO is produced during the conversion of L-arginine to L-citrulline by nitric oxide synthase (NOS). Many of the physiological effects of NO are mediated by activation of the soluble form of guanylyl cyclase. Previous study has confirmed the existence of NOS in the eyestalk ganglia of the crayfish Procambarus clarkii. The X-organ/sinus gland complex in the eyestalk is a neuroendocrine tissue. Crustacean...

Shyh-Chi Chen, 陳仕錤

碩士 / 國立彰化師範大學 / 生物學系 / 90 / Biogenic amines, functioning as eurotransmitters/neurohormones, are involved in a wide variety of physiological processes. In crustaceans, several biogenic amines, including serotonin (5-HT), dopamine, and octopamine have been found to regulate the release of several peptide hormones from a neuroendocrine tissues, X-organ-sinus gland complex, located within the eyestalks of decapods. In order to advance our knowledge regarding neuronal regulation of hormona...

楊佩

碩士 / 國立彰化師範大學 / 生物學系 / 89 /

Chia-Hui Cheng, 鄭嘉惠

碩士 / 南台科技大學 / 生物科技系 / 100 / For the biological resource development of algae, to explore algae to serve as renewable energy sources (such as biodiesel and bio-ethanol) is feasible. Especially the rapid growth of microalgae, shows efficient utilization on solar energy more than other grains. In particular, in recent years, global demand for energy, and vigorously developing the traditional agricultural crops (soybeans and corn, etc.) as raw materials for biomass energy sources, coupled with the...

Yu-Wang Su, 蘇育王

碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 107 / Tail-anchored (TA) membrane protein is safely transported to the endoplasmic reticulum (ER) membrane in mammals and yeast via the TRC40/GET3 binding factor. However, the mechanism by which the TA protein is delivered remains unclear in plants. This unique eukaryotic cell membrane transport system correctly distinguishes between different TA proteins for various organelles, including the mitochondria, chloroplast and ER TA proteins. In this experiment, we want...

Liau, Jian-Syun, 廖建勳

碩士 / 國立中正大學 / 化學暨生物化學研究所 / 103 / Green algae is one of the popular nutritional supplements in recent years. Many reports with respect to its pharmacological effects also contribute its attractions. Chlorella-11 peptide is now a known anti-inflammatory, antioxidant bioactive peptide and its primary structure is NH2-Val1-Glu2-Cys3-Tyr4-Gly5-Pro6-Asn7-Arg8-Pro9-Gln10-Phe11-COOH from isolation and purification of green algae protein (Chlorella vulgaris). In this study, the synthesized Chlorella-11 p...

Yuan-ting Hsu, 許媛婷

碩士 / 國立中山大學 / 海洋生物研究所 / 97 / This study has investigated the involvement of photosynthetic electron transport chain on the regulation of gene expression of methionine sulfoxide reductase (UfMSR) in the marine macroalga Ulva fasciata Delile.UfMSRA is from copper stress and UfMSRB ir from hypersalinity stress. UfMSRA is similar to Arabidopsis AtMSRA4 and UfMSRB is similar to AtMSRB1. UfMSRA is specific to the MetSO S-enantiomer and UfMSRB catalytically reduces the MetSO R-enantiomer. Both enzymes ...

Wu Yen-Ching, 吳彥慶

碩士 / 國立臺灣海洋大學 / 食品科學系 / 102 / The pulverized chlorella and freshwater clam were used as materials in this study. Pulverized chlorella were hydrolyzed by cellulase (Cellulase AP3) and protease (Protease N or Prozyme 6) at 50℃ for 1-5 hours. The pulverized Chlorella were hydrolyzed by Protease N and Cellulase AP3, then fermented with lactic acid bacteria up to 40h to obtain chlorella Fermented hydrolysates. The freshwater clam were hydrolyzed by protease at 50℃ for 5 hours. The inhibitory effect ...

Tung-Hei Wang, 王東海

博士 / 國防醫學院 / 生命科學研究所 / 91 / ABSTRACT Synechococcus sp. strain RF-1 is a unicellular freshwater cyanobacterium that fixes N2 aerobically and exhibits a circadian rhythm for nitrogenase activity under a light/dark regimen. Synechococcus sp. strain RF-1 also utilizes nitrate, nitrite, or ammonium for growth. Nitrate uptake in Synechococcus sp. strain RF-1 cells was experimentally induced by nitrate or nitrite and the induction repressed by ammonium. Nitrate uptake was tightly associated ...

Chia-Huan Hsu, 許嘉桓

碩士 / 靜宜大學 / 食品營養研究所 / 97 / Carcinoma is the major cause of death in Taiwan for 24 years. Leukemia is the thirteenth of cancer causes of death in adult and the first cancer causes of death in children in Taiwan. Recently, there is increasing interest in finding natural antioxidant from plants to prevent or delay leukemia development. Chlorella contains much kind of protein, amino acids, antioxidant enzymes and vitamins. This study was aimed to study the antioxidant activity of hot water extracts...

Yu-Ting Huang, 黃鈺婷

碩士 / 靜宜大學 / 食品營養研究所 / 99 / Among Chlorella extracts, hot-water soluble extract accounts for 16-18%, the polysaccharide or glycoprotein from cellulose hydrolysate accounts for 8%, leaving the insoluble part of ~60%. This study was to isolate hot-water soluble extracts (WS) and insoluble (WIS) components of Chlorella sorokiniana with various extraction conditions, including enzymatic treatments (cellulase, chitinase and tannase), different solvents (ethyl acetate and n-hexane), and fractionation (...

Jia-jin Huang, 黃佳進

碩士 / 靜宜大學 / 食品營養研究所 / 95 / Granule Chlorella is a commercial product that exhibits several biofunctions. In this study, we investigated the radical-scavenging effect of water extracts from the granule Chlorella products (GPW). Then, we studied the effects of GPW on the growth and apoptosis induction of mice leukemia cell lines (WEHI-3 cells). Subsequently, the effects of GPW on WEHI-3/BALB/c mice were examined. The results demonstrated that GPW had α,α-diphenyl-β-picryl hydrazyl (DPPH) , 2,2...

Chen-I Ju, 陳奕如

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Chlorella, rich in proteins, peptides, chlorophyll, vitamins and minerals, is a very good material for the production of functional foods. To hydrolyze the cell wall of Chlorella, a cellulase-producing bacterium, Cellulomonas sp. was isolated and used to produce cellulase for the hydrolysis of chlorella cell wall. The cellulase activity could reach 1320 U/ml after 3 day fermentation in a 5-L fermenter at 100% DO and 50 rpm. No significant difference in cellulase acti...

Hung, Chin-Chieh, 洪勤絜

碩士 / 國立臺灣海洋大學 / 食品科學系 / 107 / Lactic acid is not only the main raw material of polylactic acid, but also widely used in industry such as pharmaceutical, food, and plastics industries. Green alga Ulva sp., which contains high amount of polysaccharides and proteins, has the potential to be an alternative substrate for lactic acid fermentation. This study intends to use green alga Ulva sp. for lactic acid fermentation. The encapsulation efficiency of immobilized Lactobacillus rhamnosus BCRC 10940 a...

Hen-ShengWang, 王瀚生

碩士 / 國立成功大學 / 環境工程學系 / 106 / Toxic cyanobacterial blooms are ubiquitous in both freshwater and brackish water throughout the world. Cyanotoxins, microcystins and cylindrospermopsin for example, may cause liver and/or renal diseases, posing a great threat to human. The risks of cyanobacteria to human health have been mainly assessed for the exposure pathway of drinking water. However, in a few recent studies, inhalation of aerosolized cyanotoxins and cyanobacteria is considered to be another path...

Shu-Mi Wu, 吳書蜜

碩士 / 靜宜大學 / 食品營養研究所 / 95 / It has been demonstrated that the Spirulina showed good antioxidant capacity, anti-inflammation and neuroprotective effect. The purposes of this study were to examine the effects of Spirulina on learning and memory ability, antioxidant status, immune function and sperm quality in senescence accelerated prone mice (SAMP8). Three-month-old SAMP8 mice were divided into control and 50 mg/Kg BW, 200 mg/Kg BW and 800 mg/Kg BW water extracts of Spirulina groups, while SAMR1 m...

XU, MING-GUANG, 徐明光

碩士 / 國立臺灣大學 / 植物學研究所 / 75 /

Yi-Ching Yang, 楊宜靜

碩士 / 臺北醫學大學 / 保健營養學研究所 / 98 / Hypertension is one of the main factors causing cardiovascular disease. In vitro study has reported that some ACE-inhibitory peptides may be produced by enzymatic digestion of chlorella. In the present study, we investigated the effects of chlorella on blood pressure and cardiorenal remodeling in rats with Nω-nitro-l-arginine methyl ester hydrochloride (L-NAME)-induced hypertension. Rats were fed a diet containing L-NAME (40 mg/kg body weight) with or without chlore...

Hsin-Yi Cheng, 鄭欣宜

碩士 / 靜宜大學 / 食品營養學系 / 99 / The increase of oxidantive stress with aging has been proposed to be the contributor to the age-related hearing imparment. Over accumulation of reactive oxygen species (ROS) in inner ear was suspected to acclerate the atrophy of hair cells and spiral ganglion cells, and induce cell apoptosis. The supplementation of antioxidants has been proved to reduce the oxidantive stress, and slow the aging process. Spirulina platensis showed good antioxidant capacity, anti-inflamma...

Ying Wu, 巫熒

碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 / The inhibitory effect of Chlorella sorokiniana hydrolysates (hydrolyzed by cellulase and protease) on activity of α-glucosidase and α-amylase were investigated. When adding of 5% cellulase and 2% protease in the three Chlorella hydrolysates (pulverized cell, unpulverized cell and residue), the hydrolysate (pulverized cell) showed the highest yield being 42.0% after hydrolysis for 3 h. As the hydrolysates (pulverized cell) were hydrolyzed for 5 h, the peptide and free...

Chia-Hui Wang, 王佳慧

碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 / The inhibitory effects of Chlorella hydrolysates (hydrolyzed by cellulase and protease) against α-glucosidase were investigated and the physiological effect on regulation of blood sugar was also evaluated. The carbohydrate, soluble protein and peptide content of three Chlorella hydrolysates (pulverized cell, unpulverized cell and residue) were increased as cellulase was added to 5%. The hydrolysate (pulverized cell) showed the highest yield being 21.40%, and α-glucos...

Hui-chiao Chung, 鐘惠橋

碩士 / 國立中興大學 / 植物學系 / 87 /

吳岱融

碩士 / 國立嘉義大學 / 應用化學系研究所 / 101

黃騰毅

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 101 / Two genera and thirteen species of the Ulvaceae have been recorded from Taiwan. Based on thallus morphology, the species can be grouped into two types: 1) Blade-type: thalli composed of two-celled layers; 2) Tubular-type: thalli composed of a single celled layer. In the past, the taxonomic studies on the Ulvaceae from Taiwan were largely based on morphological observations resulting some taxonomic confusion and synonymy. In this study, the taxonomy of the Ulvaceae...

Chen, Chia-hsing, 陳佳杏

碩士 / 國立海洋大學 / 海洋生物研究所 / 86 /

Huan-yi Chu, 朱桓儀

碩士 / 靜宜大學 / 食品營養研究所 / 96 / It was demonstrated that chlorella has the effect of antioxidation , antitumor, lowering the blood fat and reducing blood pressure. The purpose of this study was to investigate the effect of different types of chlorella on learning and memory ability and antioxidative status in senescence-accelerated mice. 3 and 6-month-old senescence-accelerated male and female mice were divided into four groups (n=15 per group) : control group, commercial mechanical chlorella powder...

Jian-Gu Lin, 林建谷

碩士 / 靜宜大學 / 食品營養研究所 / 96 / Chlorella vulgaris is one of the popular healthy materials in natural algae, with healthy efficiency potentially relating to glycosidase (α-glucosidase, β-glucosidase and β-glucuronidase) inhibition. Therefore, this study was to investigate the isolation, preparation, characterization, and glycosidase inhibition of various fractions from Chlorella vulgaris. The samples included: hot water extract of Chlorella (WS), enzymatic hydrolyte of Chlorella hot water insoluble (...

Xian-Hui Peng, 彭顯惠

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / A cellulase-producing bacterium, could form a clear zone on carboxy methyl cellulose (CMC) agar plates and designated as C-6, was isolated from soil and identified to be a Cellulomonas sp.. The optimal conditions for its growth were pH 6.8 in nutrient broth with 1.0 % carboxy methyl cellulose (CMC) at temperature of 30 oC and 150 rpm of shaking speed. The maximal cellulase activity was obtained after 96 hr incubation at 30 oC. In order to hydrolyze the cell wall of C...

Ying-Jhih Chen, 陳盈志

碩士 / 國立中興大學 / 食品暨應用生物科技學系 / 94 / The previous studies on the determination of heterotrophic cultivation conditions under dark environment in shaken flasks for a useful algal strain, Chlorella sorokiniana NCHU-6, was continued in this study. Medium optimization using experimental designs was performed further in this study in order to increase further the growth yield and cellular chlorophyll content. Moreover, the optimum medium obtained was examined by using the 5-L table-top fermentor without ...

In-Jye Tsai, 蔡英杰

碩士 / 臺北醫學大學 / 保健營養學系 / 94 / Due to the improvement of life standard, increase of alcohol consumption and obesity. The incidence and prevalence rates of gout among Taiwanese population keep a tendency of growing. The drug for hyperuricemia, Allopurinol, causes serious sensitive reaction to susch patients. Therefore, it would be optimal to moderate the level of higher blood uric acid by the way of adjusting the composition of diet. The effect of oral administration of Chlorella extract with diet ...

Hung-Pin Shu, 許宏賓

碩士 / 國立中興大學 / 生命科學院碩士在職專班 / 93 /