Chia-Xi Chen, 陳佳熙

碩士 / 國立嘉義大學 / 食品科學系研究所 / 95 /

林呈翰

碩士 / 國立嘉義大學 / 水產生物學系研究所 / 95 /

Ming-Yuan Lin, 林明轅

碩士 / 臺灣大學 / 漁業科學研究所 / 96 / IGF2 mRNA binding protein 2 (IMP2) was first identified as an auto-antigen p62, which was aberrantly expressed in the human hepatocellular carcinoma (HCC). The roles of IMP2 in HCC development, from chronic liver disease such as fatty liver, cirrhosis to HCC, are still poor understood. Up to now, no any animal model of IMP2 have been reported. In this study, I first intended to establish IMP2 transgenic zebrafish lines by Tol2 transposon-mdiated transgenesis to study t...

Sheng-Fei Chuang, 莊盛斐

碩士 / 國立彰化師範大學 / 生物技術研究所 / 97 / Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. We have expressed zebrafish Prx2&6 in Escherichia coli. BL21(DE3). The recombinant zebrafish Prx6 has been purified in affinity chromatography of HisTag binding resin and the yield Prx2 and Prx6 about 0.05 and 0.025 mg/per 100ml. We have do protein identification with MALDI-MS/MS. Purified Prx6 lacks emzyme activity because of oxidation of its single conserved cysteine. Prx6 activity wa...

Wu Chia-Jen, 吳嘉仁

碩士 / 國立臺灣大學 / 漁業科學研究所 / 90 / Freshwater (FW) teleosts are capable of acclimating to seawater (SW) following such a transfer from FW. However, their osmoregulating mechanisms are still unclear particularly those in the brain. Previous study shows that Na+-K+-ATPase activity is coincidental with creatine kinase (CK) activity during SW (25 ppt) transfer. The present study was made attempt to examine acute changes in phophatidylinositol -3 kinase (PI-3 kinase), β-catenin, β-spectrin, CK-Mi, CK-B and...

Gong, Shu-Fan, 龔書凡

碩士 / 輔仁大學 / 西班牙語文研究所 / 82 /

Wen-Kai Yang, 楊文凱

碩士 / 國立中興大學 / 生命科學系 / 93 / Sailfin molly, an exotic fish in Taiwan, is an euryhaline teleost which can survive in either freshwater or seawater environments. Because the mollies are aquarium fish, they have a few variations in appearance. In order to achieve accuracy of the experiment, we performed measuring morphometric characters and contrasting the sequences of mitochondrial NADH dehydrogenase subunit 2 (NADH2) to identify species. The results indicated that the sailfin molly is the most simi...

Li-Hua Chiu, 邱麗華

碩士 / 輔仁大學 / 食品營養學系 / 93 / Chinese soft-shelled turtle (SST) is one of highly economic products in cultivation in Taiwan, it have many kinds of physiological functions. This study was to investigate the antihypertensive effect and inhibition of angiotensin converting enzyme (ACE) activity of hydrolysate of SST powder (PCT-SST) derived from hydrolysis of pepsin, trypsin and chymotrypsin. The SST powder showed limited inhibition of ACE (IC50 = 16.7 mg/mL). The inhibitory activity of ACE in hydrolys...

Yu-Yung Jian, 簡裕勇

碩士 / 逢甲大學 / 土地管理所 / 93 / Abstract It is necessary to think how the community tourism development should use its local precious resources to create its activity value and avoid being substituted by the external culture. Nevertheless, many issues were encountered when developing community tourism, they were: a lack of integration and preservation in community resources, residents seldom participate in tourism activities, competition among tourism operations, lack of benefit sharing and feed-b...

Chang, Kaung-Huey, 張光慧

碩士 / 國立中興大學 / 分子生物學研究所 / 86 / Filamenous phage ΦLf specfically infects xanthomonas py campestris ,the causative agent of black rot in crucifers ΦLf contains a citcular single-stranded DNA genome of 6,008bp ,uses a replicative form RF as the intermediate of replication, and manifests a non-Lytic life cycle . Like Ff phages (fl,fd and M13), ΦLf has similar biological proerties and genomic organization i,e,in the order gII-gX-gV-gVII-gIX-gVIII-gIII-gVI-. AII the open reading frames for ΦLf have al...

Yi-Chun Lou, 羅昱淳

碩士 / 國立高雄海洋科技大學 / 漁業生產與管理研究所 / 94 / Taxonomically, Selachomorpha is included in subclass Elasmobranchii under class Chondrichthyes, being divided 8 order. The phylogenetic relationships within Selachomorpha are still in vague. Furthermore, the systematics of First order and order remains controversial, we reconstruct their molecular phylogeny by using mitochondrial DNA sequences. Most of the specimens were collected in Nan-Fang-Ao and Cheng-kung Port from September 2004 to August 2005 in this st...

Chuian-Fu Ken, 耿全福

博士 / 國防醫學院 / 生命科學研究所 / 90 / Cu/Zn-superoxide dismutase (Cu/Zn-SOD) and catalase cDNA were cloned from zebrafish using RACE-PCR technique; both of the cDNA coding regions were successfully expressed in Escherichia coli. The wild type Cu/Zn-SOD（ZSOD1） was purified by His-tagged technique and was found labile than plant Cu/Zn-SOD. In an attempt to enhance enzyme stability, a mutant enzyme (ZSODC7A, Cys-7 to Ala) was obtained by site-specific mutagenesis and showed higher thermo-stability than wild-...

Jung-Chen Hsieh, 謝榮振

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 93 / Gonadotropins (GtHs), follicle stimulating hormone (FSH) and luteinizing hormone (LH) play a major regulatory role in reproductive process. They both contain a common glycoprotein α subunit (GtHα, common α) and a hormone-specific β subunit, such as FSHβ and LHβ, respectively. Zebrafish (Danio rerio) was used as a model fish and the technique of RNA interference (RNAi) was employed to knockdown these three glycoprotein subunits. Three short-hairpin RNA (shRNA) expre...

Li Chia-Jung, 李佳榮

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 96 / Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide and belongs to the vasoactive intestinal peptide/secretin/glucagons/GHRH superfamily. PACAP is present in two biologically active forms with 38 and 27 amino acid residues, designated PACAP38 and PACAP27, respectively. The diverse functions of PACAP including the regulation of apoptosis, metabolism, endocrine and immune systems have been widely studied in mammals, however, literature on the...

Meng-Chen Wu, 吳孟真

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / This study investigated the antioxidative activity of freshwater clam, Daiwa freshwater clam, and I-Tiao-Gung extracts together with the influences of them on the liver antioxidant enzyme activity of tilapia at different concentrations. Hot water (FC-HW), hot water-80% ethanol (FC-HW-E), and 80% ethanol (FC-E) extracts were prepared from freshwater clam. The Daiwa freshwater clam samples included hot water extract (DWFC) and its 80% ethanol extract (DWFC-E). I-Tiao-G...

Chia-Ling Wu, 吳佳玲

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Abstract The purpose of this study was to evaluate tilapia as an animal model to prescreen anti-cold stress bioactivities and to look for a biomarker to detect changes physiological caused by dietary intake of hot-water extract of a shellfish, freshwater clam (FC), short-necked clam (SC), oyster (OY) or 95% ethanol extract of I-Taio Gung (GTE) before using rodents or other mammalians as experimental animals. Tilapia (141.1 ± 24.0 g) fed 2% FC, SC, OY diet for 3 month...

Hsin-I Liu, 劉興懿

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / Abstract Aiming at Grouper Iridovirus of Taiwan (TGIV), this research discusses the correlation among temperature, viral pathogenicity and virus-induced apoptosis. First, grouper were intraperitoneal injected with TGIV in concentrations of 103.5 TCID50/fish, and then reared at 18℃, 25℃ and 32℃ respectively. After 2 weeks, the cumulative mortality of virus-infected fish under the above 3 water temperature were 45%, 100%, 78% respectively and control fish were not fo...

Sie Lin Choo, 朱曦林

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / Member of the T-box family of transcription factors, tbx5, expresses in the heart, pectoral fins and eyes of zebrafish during embryonic development. In zebrafish, injection of tbx5 morpholino antisense RNA caused changes of heart conformation, defect of heart looping, pericardium effusion, dropsy of ventral position and decreased in heart rate. Meanwhile, expression of cardiac myogenesis genes amhc, vmhc and cmlc2 was decreased dramatically by the knockdown of tbx5 ...

kun-Chi Huang, 黃崑棋

碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 /

Shan Su, 蘇珊

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 95 / Apoptosis is termed as programmed cell death, which plays a crucial role in the development of central nervous system (CNS). Some pro-apoptosis proteins such as DAPK family, Daxx and Par-4 play an important role in neuronal cell death. These proteins was found to interact with each other. To study the function of pro-apoptosis proteins in zebrafish development and neuronal cell death, we first cloned zebrafish ZIPK, Daxx and Par-4 cDNAs, and the zebrafish DAPK whic...

Min-Fen Hou, 侯閔分

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 95 / The main function of sperm is transporting genetic material into egg. The activity of sperm influences the ability of eugenesis. Sperm moves on by the wiggle of the tail, and there are three parts of sperm-tail. The central one is axoneme which is close to outer dense fiber, and the outside part is fibrous sheath. The promoter of sperm-tail has high specificity in sperm. We made GFP fusion with it, and use the GFP-Sperm-T fusion constract for transgenesis zebrafish...

林啓興

University of Macau / Institute of Chinese Medical Sciences

Chung-Yeh Sun, 孫忠業

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / The purpose of this study is aimed to know the effect of dietary protein on the growth performance and immune response of longtooth grouper (Epinephelus bruneus) and the effect of dietary lipid on the growth performance and body composition of pearl grouper. In the first trial, fish meal was used as dietary protein source, fish oil was used as dietary lipid source, α-starch and cellulose was used as energy regulater. Duplicate grouper of longtooth grouper fed is...

You-Hsin Chang, 張友信

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / PART ONE In our previous studies, we detected a high expression of heat shock cognte 70 (HSC 70) in developing zebrafish and the spontaneous HSC 70 expression was found to be controlled by translational regulation. The purpose of this study was to explore the mechanisms of the translational regulation that produce the spontaneous HSC 70 synthesis. RT-PCR-PAT assay indicated that the poly(A) tail of hsc70 mRNA isolated from 12 to 120 hpf zebrafish carried the sa...

WANG,PEI-QI, 王珮琪

碩士 / 國立中興大學 / 獸醫研究所 / 79 /

Mei-Yun Huang, 黃美韻

碩士 / 淡江大學 / 生命科學研究所碩士班 / 94 / D-amino acid oxidase (DAO) is a FAD-containing enzyme which has been characterized in microorganisms and animals. On early periods, the studies of DAO were focused on analysis of enzymatic activity, large-scale production of DAO enzyme, to produce antibiotic and in treatment of patients who had amino acid metabolism disorders. Recently, the distribution and physiological functions of DAOs have become an important issue to be addressed. Sodium benzoate (SB) is a comp...

Chiu, Man-Ting, 邱曼婷

碩士 / 國立屏東科技大學 / 水產養殖系所 / 102 / The study was to investigate dietary arachidonic acid (ARA)-rich fungal meal utilization and their effects on growth, immune responses and fatty acid profile of juvenile grouper, Epinephelus coioides. In Experiment 1, basal diets containing 20% soybean meal were supplemented with 1% and 5% fungal meal (FS + 1% D and FS + 5% D). All fish meal (F) diet and basal diet (FS) without fungal meal was used for comparison. Triplicate groups of 12 fish (initial wt: 11.56 ± 0...

陳喬富

碩士 / 國立海洋大學 / 水產生物技術研究所 / 90 /

Yu-Chen Lin, 林育珍

碩士 / 臺灣大學 / 口腔生物科學研究所 / 98 / The Tol2 element was isolated from the genome of the Japanese medaka fish. The Tol2 transposable element belongs to the hAT family of transposons including hobo of Drosophila, Ac of maize, and Tam3 of snapdragon. Transposons have been used as powerful tools for genetic studies in Drosophila and other model organisms. Such a tool had, however, not been developed in zebrafish. This is because no active DNA-transposable element has been found from the zebrafish genome. ...

Yang, Jer-yen, 楊哲彥

碩士 / 國立海洋大學 / 生物技術研究所 / 85 / Transferring the heterologous gene into the embryos for getting the transgenic animals is a good way not only to research the function and expression of the gene but to improve the species. By the ways like microinjection, particle gun and electroporation, we introduce tilapia IGF-I, (Beta-galactosidase and GFP gene into the zebrafish embryos so that we can detect the tilapia ...

Huang, Li-Wen, 黃莉雯

碩士 / 國立臺灣海洋大學 / 食品科學系 / 108

Lin, Yi-Wen, 林怡彣

碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 107 / Nervous necrosis virus (NNV) is the causatived agent of viral nervous necrosis symptom of more than 120 species of both marine farmed and wild larvae and juvenile fish. NNV belongs to the beta subgroup of the virus family Nodaviridae. The nodavirus genome consists of two positive-sense single-stranded RNA molecules. RNA1 encodes an RNA-dependent RNA polymerase (RdRp) , while RNA2 encodes the viral capsid protein (CP). In addition, a subgenomic RNA transcribed ...

Ma, Zhong-yang, 馬忠陽

碩士 / 國立臺灣海洋大學 / 應用經濟研究所 / 107 / Food poisoning incidents and food safety issues have always been one of the topics of concern to all countries. These issues are highly concerned in Europe and the United States. With the recent emergence of food safety problems and food poisoning incidents, food safety and hygiene control are highly regulated. Especially in recent years, the food safety of seafood products has increased due to the increase in related incidents, and the issues related to seafood p...

Hsieh, Hsin-Yin, 謝昕穎

碩士 / 國立臺灣海洋大學 / 電機工程學系 / 107 / Currently, all information about the fishes in the National Museum of Marine Biology &Aquarium (NMMBA) in Taiwan is provided through on-site explanation boards or audio tours. However, with so many different fish species in a large fish tank, the explanation board has to be enormous and takes up a lot of space, and an audio tour could also be very manpower demanding. A potential solution to this problem is to develop a fish identification system which can identify ...

Zhang, Pei-Hua, 張沛華

碩士 / 國立臺灣海洋大學 / 資訊工程學系 / 107

Su, Yu-Pu, 蘇昱溥

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 107 / As the global climate change, the water resource reduces. Developing tilapia seawater culture can reduce the consumption of water resource and increase the production of tilapia. Seawater tilapia has the advantages of good flavor and good meat quality. Produce of tilapias in brackish water enhance the economic value of tilapia farming. The purpose of this study was to develop molecular markers of salinity tolerance regulated genes by using transcriptome sequencing ...

Huang, Rong-Da, 黃榮達

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 107 / This study was divided into two parts. The first part presented biological and economic information of the milkfish farmers in Taiwan. Farm survey on the milkfish was conducted and the studied variables included biological variable, cost variables and profitability variables. The analysis on the milkfish farming was divided to eight groups based on two major factors including farming strategies, and geographical location. Different statistical methods were applied ...

Chang, Chi-Hsuan, 張綦璿

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 107 / 英文摘要 Blue marlin (Makaira nigricans) play an important ecological and economic role in pelagic ecosystems, exerting top down control on their prey in waters off eastern Taiwan. Rich food resources were found in the Kuroshio Current, but little is know about the feeding ecology of the area. This study aims to understand the trophic level of the blue marlin and the relationship to ecology of Kuroshio Current. Results from the index of relative importance (IRI) ...

Kang, Chia-Hui, 康家慧

碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 107

Yu-ying Li, 李鈺瀅

碩士 / 國立中山大學 / 中國文學系研究所 / 106 / This study will explore the records of whales from Chinese literature, and classify them according to their historical materials. Conducting a study of the whale culture using the " Circuit of Culture " approach of cultural studies. First, focus on the whale appearance, haunting and economic value. Then, through the image description of the whales by the ancients, sum up the interpretation meaning of the whale in literature, and the cause of the formation of the le...

MingYu, 游敏

碩士 / 國立成功大學 / 生物科技與產業科學系 / 107 / Tumor necrosis factor-α (TNF-α) is known as a pleiotropic cytokine in the innate immune system. However, few studies focused on the investigation of TNF-α in teleost. In previous study, two types of TNF-α in orange-spotted groupers (Epinephelus coioides) have been identified, named grouper TNF-α1 (gTNF-α1) and grouper TNF-α2 (gTNF-α2), respectively. Both of proteins shared 35.6% amino acid sequence identity. In the phylogenetic analysis for teleosts, they were cl...

Jhih-Ling Huang, 黃芷翎

碩士 / 國立臺灣科技大學 / 材料科學與工程系 / 104 / In this study, polylactic acid (PLA)/scales powder (SP) composites were prepared by melt blending. The SP content of composites are from 0 to 50 wt%. The thermal stability and the residual amount of the PLA and the PLA/SP composites were studied by using a Thermo gravimetric Analyzer. Moreover, differential scanning calorimeter (DSC) analysis at a rate of 2 ℃/min to discussion melt crystallization behavior, melting behavior and isothermal crystallization kinetics...

Nien, Yu-Ling, 粘育苓

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 104 / This study researches on the early life history of 3 cryptic species of Mugil cephalus juvenile in estuaries around Taiwan. Present study collected monthly juvenile samples from November 2013 to March 2014, and firstly discriminates the mullet juvenile individuals collected in varied estuaries by genotyping for understanding their spatial- temporal distribution. Then analyze otolith daily age and microchemistry to investigate the early life history of differe...

Ting-Shuan Wu, 吳亭萱

博士 / 中山醫學大學 / 醫學研究所 / 101 / Citrinin (CTN) and Patulin (PAT) are secondary metabolites secreted by fungi that frequently contaminate food and feed. The organotoxicity of CTN and PAT has been reported in several animal models, and kidney is the major target organ of these two toxins. However, their early developmental toxicities to embryos remain unclear. Since, zebrafish shows the characteristics of embryo transparency, rapid development, and similarity to human, the zebrafish embryo was used as...

Chao,Hung-Hui, 趙虹惠

碩士 / 國立臺北藝術大學 / 美術學系碩士在職專班 / 107 / Since the early days of my creative consciousness, I have always paid close attention to space and urban issues. After urban field projects and some creation practice experience about internal and external exploring of cities, in addition to examining the socially appropriate positioning of my creations, there has always been an essential reflection on "how the city is shaped". The idea of this creative project started in 2016 when I moved to the Xiao-xi-men vi...

Yu Chang, 張鈺

碩士 / 慈濟大學 / 生物化學碩士班 / 107

Chun-Shu Lin, 林群書

博士 / 臺北醫學大學 / 臨床醫學研究所 / 107

HUANG, PIN-QI, 黃品錡

碩士 / 朝陽科技大學 / 景觀及都市設計系 / 107 / In recent years, the rapid development of global cities has led to a significant reduction in the area of agriculture. How to use urban infrastructure to increase urban micro-farming areas has become an important part of create sustainable cities. In addition, the drastic climate change has affected the unstable production of food and caused food supply crisis. Therefore, many countries continue to promote sustainable agriculture to ensure the stability of the worl...

CHUANG, YU-CHEN, 莊又臻

碩士 / 南華大學 / 文化創意事業管理學系 / 107 / The way in which Wuhu B&B needs to develop a proper management strategy; most operators do not know how to make good use of the rich natural and human resources available, so they cannot effectively develop appropriate management strategies, but the efficiency is also poor, making the characteristics of the B&B impossible. It is manifested and does not attract consumers, so that it loses its competitive advantage. Most of the hotel operators are self-employed. Ther...

Peng, Hui-Wen, 彭慧文

碩士 / 國立臺灣師範大學 / 生命科學系 / 104 / Environmental salinity variations have been proved to stimulate Na+-K+-ATPase (NKA) activity to regulate homeostasis in intestine of euryhaline teleosts. In addition liver can conduct metabolism for supplying intact energy demand. The non-essential amino acid, glutamate, is the most abundant amino acid in organisms. In euryhaline teleosts, glutamate accumulation and related metabolism have been observed in several vital tissues under salinity challenges. However, mo...