Mei-feng Huang, 黃美鳳

碩士 / 國立中山大學 / 生物科學系研究所 / 104 / Because establishment of blood vessels in vertebrates is highly conserved in evolution, and extremely important in embryogenesis, zebrafish have become a widely accepted model that has been used to identify many genes involved in vascular development. Using zebrafish, we found that transcription factors islet2 and coup-TF1b act via the Notch signaling pathway to control vein and intersegmental vessel (ISV) growth. We further investigated the downstream targets of i...

Sen-yuan Chung, 鍾森元

碩士 / 國立中山大學 / 生物科學系研究所 / 104 / Quinone (Quinone) is a general term for a class of organic compounds containing conjugated cyclohexadiene-dione (conjugated diene cyclohexene), or cyclohexadiene dimethylene (cyclohexadiene dione dimethylene) structure. Previous studies have pointed out that quinone has biological and pharmacological activity, thus many derivatives of quinone were developed for clinical use. Akt is also called Protein Kinase B (PKB). Akt signal pathway plays an important role in ce...

Yen-Hui Kung, 龔彥回

碩士 / 國立臺灣大學 / 應用力學研究所 / 104 / There are lots of vortex energy in the nature, for example, the known tornadoes and the whirlpool scaring swimmer. I want to explore new method of vortex energy harvesting. According to Inverted-flag experiments and the experiment of flapping fish model behind semicircular cylinder, I produce the Karman vortex street by triangular prism and put an inflexible inverted flag which is free-rotating. Change free stream velocity, length of flags and the distance between i...

Huang, Jing-Yi, 黃靜怡

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / Tilapia is the high economically important as food fish species for aquaculture in the world. It has good environmental resilience, strong reproductive capacity, high yield and high survival advantages. Growth trait is an important economic characteristic of selective breeding for aquaculture breeders. However, the phenotypic variation of the normal somatic growth of fish is a remarkably complicated biological phenomenon that involves interactions between external ...

Wu, Sz-Ching, 吳思磬

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / In this study, we’re focusing on the grouper iridovirus (GIV), study the changes of other pathogenic virus such as nerve necrosis virus (NNV) and Taiwan grouper iridovirus (TGIV) for the propagation of in vitro and in vivo test and cause of death in histological differences during the period of co-infection. Therefore, we divided six groups included group of GIV, NNV, group of low virus titer GIV with high virus titer TGIV (GlTh) , group of low virus titer GIV with...

Chen, Kuang-Yao, 陳光耀

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 103 / Till 2013, there are 27 families 56 genera 69 species of freshwater and estuarine fishes including 9 families 12 genera 12 species of primary freshwater fishes had been recorded from the Kinmen islands. The current project would process the native population monitoring of the endangered cyprinid species, Metzia mesembrinum as well as the native, rare inland-water fish ecological survey of Kinmen islands, Fujian Province, R.O.C. The native habitats with the fish po...

Min-Hung Tsai, 蔡旻宏

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 97 / Grey mullet (Mugil cephalus) is one of the most important economic fish in the coastal waters of Taiwan. They are divided into at least two groups (migratory and residential type). To find out the level of genetic divergence and gene flow between them, we use mtDNA 16S RFLP to identify migratory and residential type and investigate genetic variations of the 5 microsatellite loci. We analyze 173 grey mullet fishes from 3 different locations of Taiwan, where are PuD...

Pei-Ru, Tsai, 蔡佩如

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 97 / Cadmium, a heavy metal, is known to influence the offspring through maternal transfer. Most studies focus on the maternal cadmium influences the relative heavy metal tolerance and production of offspring. Researches on the physiological mechanism of the infecting-cadmium parental generation to offspring and influence on the specific gene expression were nevertheless numerous. Therefore, the aims of this study were to confirm the effects of different doses of cadmi...

Tsung-Che Hsieh, 謝宗哲

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 93 / In embryogenesis of zebrafish, primordial germ cells (PGCs) formed four clumps before 1000-cell stage. PGCs would migrate and form two bilateral rows of cells close to the future gonad site. We micro-injected with a BstBI-cut pZBrBISRK plasmid and generated 5 transgenic line. One of which, ZBrBISRK2, having 45 % heredity rate, the highest among 5 transgenic lines, was selected. The nanos mRNA was detected not only in future gonad but also in head, trunk, and tail...

Hsin-Yi Chen, 陳欣宜

碩士 / 中山醫學大學 / 營養科學研究所 / 92 / In this study, the search tool BLAST (Basic Local Alignment Search Tool), based on NCBI (National Center for Biotechnology Information) was used against nucleotide and protein databases. The analysis found a hydroxysteroid dehydrogenase (HSD) of zebrafish (NCBI accession numberAL672176/ CAD54662) and human (NCBI accession number NM_014234/ NP_055049). The HSD cDNA of zebrafish and human were successfully amplified with a continuous open reading frame of 771 bps and 7...

Kuan-Lun Huang, 黃冠綸

碩士 / 國立臺灣大學 / 生化科學研究所 / 94 / The ephrins and their Eph receptors are membrane-bound molecules that mediate cell-to-cell signals implicated in the regulation of cell migration and development of nervous system. In many cases, ephrins act as negative regulators that stimulate growth cone collapse and inhibit neurite outgrowth. In this study, to investigate the effects of ephrinBs on neurite outgrowth, I cloned ephrinB genes of zebrafish including ephrinB1, B2a, B2b, B3 and driven them by two neura...

Hao-Seong Hoi, 許巧雙

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 94 / Myf5 is one of myogenesis regulatory factors (MRFs), which plays roles in the specification and differentiation of muscular cells during myogenesis. The expression of myf5 is in a somite- and stage-specific manner under a fine-tuned control mechanism. However, the molecular mechanism of repression of myf5 is still unknown, although repressive element within intron I (+502/+835, I300) of zebrafish myf5 was reported. In this study, we microinjected the upstream reg...

Hui-hsin Chen, 陳慧欣

碩士 / 國立臺灣大學 / 環境工程學研究所 / 94 / Fishery products will accumulate mercury via their food source in contaminated water bodies, and then be consumed by people, and causing the injury to human bodies. This study is aimed to develop the method predicting the mercury concentration in fish and the human risk. The bottom mud samples, water sample, fish, specimen were collected during September 2005 to March 2006. The results showed that the total mercury concentrations of Elops machnata was up to 3.26±0.7...

Hue-Ling Chen, 陳惠玲

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 94 / The hepatocyte nuclear factors-3 (HNF-3) family members HNF-3, HNF-3 and HNF-3 are hepatocyte-enriched transcription factors, they play important roles in controlling development, cell differentiation, organogenesis and in the regulation of metabolism. In our previous study, the expression of insulin-like growth factor-I (IGF-I), IGF-II, HNF-1a, -1b, and -3bwere detected in the liver and gonads of tilapia, and the expression level of HNF-3b was higher than oth...

Wei-Ting Guo, 郭瑋婷

碩士 / 國立宜蘭大學 / 園藝學系碩士班 / 101 / Epinephelus spp. (i.e., groupers) are aquaculture products that are critical to the Taiwanese economy. Recently, the grouper iridovirus (GIV) infection has caused considerable fatality rates in groupers. A commercially available inactive vaccine administered through intraperitoneal injection currently provides effective protection to groupers; however, to combat frequent occurrences of the GIV epidemic, a more convenient and economical vaccine strategy is required. ...

Hong-Yi Lin, 林宏益

碩士 / 國立宜蘭大學 / 園藝學系碩士班 / 101 / Groupers are considered to be the most economically important edible, cultured marine fish in Taiwan. Iridovirus has been identified as the most important pathogens of grouper culture causing major problems at the fingerling stages and severely affected the development of Taiwan grouper aquaculture industry. Now the researching and prevention of iridovirus disease is an urgent issue. This thesis mainly focused on the antibodies production and genes expression of G...

Chia-ChienWu, 吳佳倩

碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 99

Chia-LinChang, 張佳琳

碩士 / 國立成功大學 / 生物化學研究所 / 99

Pei-YingChang, 張霈瑩

碩士 / 國立成功大學 / 水利及海洋工程學系碩博士班 / 99

Chuan-RueiJhao, 趙傳睿

碩士 / 國立成功大學 / 水利及海洋工程學系碩博士班 / 99

Tsuei-han Wu, 吳翠函

碩士 / 靜宜大學 / 食品營養研究所 / 98 / To quantify the dietary manganese (Mn) requirements for juvenile hybrid tilapia, Oreochromis niloticus × O.aureus, organic Mn-peptide was added to the basal diet at 0, 2, 4, 8, 10, 15 and 30 mg Mn/kg diet providing 2.94, 5.34, 7.04, 11.20, 13.03, 18.39, 34.96 mg Mn/kg diet, respectively, 7.28 mg Mn/kg diet supplemented with inorganic MnSO4 was used as reference. There were total of 8 experimental diets, each was fed to triplicate groups of fish (initial body weight 1.0...

楊裕宏

碩士 / 國立海洋大學 / 水產養殖學系 / 88 / Abstract In researching the regulation and function of the gene. it is a powerful method to culture the animal which was transferred a part of an foreign gene when it was still a gamete or zygote. It is also the best method to make the species better. We transferred exogenous gene pTracerTM-CMV-VP2 into the sperm of Haliotis divorsicolor using the electroporation、the incubation、the liposome-DNA injection and the liposome method，and then the sperm was u...

Yi-Hsien Chiu, 邱宜賢

碩士 / 國立海洋大學 / 水產養殖學系 / 88 /

Chi I Lee, 李芷懿

碩士 / 國立海洋大學 / 水產養殖學系 / 88 / Since 1980, technique of transgenic was developing and the successful examples of transgenic animal were more than before. Transgenic animal such as: mouse, Dorsophila and C. elegans were had been succeeding. In recent years, we use fish as a model animal for transgenic and because of their short life cycle, large number spawning and easy to observe their transparent fertilized eggs, especially zebrafish. We found that transgene in fish was unstable and wil...

Yi-Feng Kao, 高翊峰

碩士 / 國立海洋大學 / 水產養殖學系 / 88 /

Chen wen-hsiung, 陳文雄

碩士 / 國立海洋大學 / 水產養殖學系 / 88 / The objectives of the study were to investigate the effects of cold stress on catecholamines, cortisol and immune responses in tilapia (Orechromis aureus). The study was divided into five parts, 1) set up catecholamines assay, induce and purify tilapia IgM antibody, 2) effects of chronic and acute cold stress on catecholamines, cortisol and IgM in tilapia, 3) effects of different stages of acute cold stress on catecholamines, cortisol and IgM in tilapia, 4) in vivo ef...

Liao Chia-Wei, 廖家葦

碩士 / 國防大學政治作戰學院 / 政治研究所 / 102 / The military is an extension of politics and its purpose is to ensure a nation’s core interests—national security and development—are safeguarded. Under this premise, most nations must take into consideration various psychological and material factors to achieve its strategic objectives. As a rising power expanding outwards, China has to increase her contact with major powers like America and Japan because of her political system, historical and geopolitical relat...

Wu Yi-Jin, 吳宜瑾

碩士 / 國立臺南藝術大學 / 音像動畫研究所 / 96 /

Chih-Yu Yang, 楊芷毓

碩士 / 國立臺灣大學 / 漁業科學研究所 / 99 / Fish will change with and increases along with the environmental condition, the community fades and the age structure. Research in fish’s age structure, sex ratio, density, the sphere of action as well as the environment factor influence are called the ecology population. Nanzaigou stream was located at the tributaries of Shihmen Reservoir, it was often over-flooded and caused serious damage along the stream side, so it was restored by the natural ecological engineer...

Zeng,Jyun-Hao, 曾俊豪

碩士 / 南開科技大學 / 電機與資訊工程研究所 / 99 / The demand on oxygen for High-density aquaculture depends on the species, stocking density, body weight, water temperature, and fish in the state vary. In addition to oxygen produced by photosynthesis the oxygen-increasing machine is necessary for maintaining the pool temperature to produce enough oxygen. In general the volume of dissolved oxygen required for the survival of is 5 ppm or more. When the volume of dissolved oxygen is less than 5 ppm will die. The mai...

Hsiao-Yun, 鄭曉芸

碩士 / 中山醫學大學 / 生物醫學科學學系碩士班 / 95 / Homeobox genes are transcription factors that play important roles in the regulation of embryonic development. Iroquois homeobox-like 1 (Irxl1) is a new member of the TALE superfamily of homeobox genes that is most-closely related to the Iroquois class. Recent studies in mice showed that Irxl1 mRNA was highly expressed in craniofacial mesenchyme, somites, limb buds, kidneys and testis cords; however, its function has not been well characterized. To investigate th...

Hseng-Hsiang Huang, 黃聖翔

碩士 / 國立臺灣大學 / 漁業科學研究所 / 96 / Grouper iridovirus (GIV), a double strand DNA virus, encoded 139 open reading frames (ORFs) in its 140 kb genome size. Iridovirus mainly attack immune system such as spleen and kidney of host. The research of virus gene expression profile upon infection had been revealed. However, investigation of host gene expression during virus infection is still unknown. In order to understand virus infection mechanism and gene expressing profile of host, we have constructed GIV-...

Ping-Hsi Yang, 楊秉熹

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 96 / Zebrafish is an excellent alternative animal for using as a bioreactor because of short generation time, light-induced spawning, high fecundity, easy manipulation of gene transfer, and cheap culture system. Bovine lactoferricin possesses antimicrobial activity against a wide range of microorganisms, antiviral, antitumor and immunomodulatory activities. In this study, we generated transgenic zebrafish as a bioreactor to produce recombinant lactoferricin through wh...

Yuan-Fong Chang, 張元鳳

碩士 / 淡江大學 / 生命科學研究所碩士班 / 95 / MRF4 belongs to the family of myogenic regulatory factors（MRFs）and participates in the differentiation and proliferation of myoblasts. Previous studies in Zebrafish indicated the existence of two different types of MRF4 gene, namely MRF4a and MRF4b. This situation is the same as the three other members of MRF family: Myf5, MyoD, Myogenin of which they share a conserved bHLH domain. Other studies indicated another functional protein sequence is present at the carboxy...

Chen, Jin Ju, 陳金菊

碩士 / 國立中興大學 / 食品科學研究所 / 84 /

Chou Ming-yi, 周銘翊

碩士 / 東海大學 / 生物學系 / 87 / Abstract Calcium ion is essential to larvae development and growth. Most of previous studies on calcium balance examined only the calcium influx and it was assumed that calcium efflux remained minimal. No comparison was done in terms of the rates of modulation between influx and efflux. This was, in part, because the technical difficulty in measuring calcium efflux in fish larvae. The present study, using different calcium concentration as environmental fac...

Hsin-Chih Hsieh, 謝欣芝

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 103 / In order to understand the effect of different dietary proteins on the fatty acid composition of tilapia, there experimental diets containing extracted soybean protein, defatted fish protein or casein were fed to triplicate groups for 8 weeks in the first place. After the growth trial, all fish of three groups of tilapia were starved for 24 hours, and then 6 fish in each group were taken as samples. Liver, abdominal muscle, back muscle, tail muscle and adipose...

ZHANG,MING-HUANG, 張銘煌

碩士 / 國立臺灣大學 / 獸醫研究所 / 79 /

CHEN,LU-HONG, 陳陸宏

博士 / 國立臺灣大學 / 農業化學研究所 / 79 / Vibrio parahaemolyticus(腸炎弧菌) 是嗜鹽性微弧狀桿菌，常在海鮮食品中檢出， 是國內常見之食物中毒病原菌。 為尋求快速檢驗方法，本研究由七段低聚核 酸中，以收集之95株V.parahaemolyti- cus 為檢測對象，其中自臨床檢體分離的有31株，自食品分離的有64株，篩選到一段 具有26個鹼基之5''-GCT-AAC-TTT-GTT-GGT-GAA-GAT-GAA-GG-3''序列，經 P 標識後作 為探針 (稱為probe 5)，以菌落雜交之方法，可檢測V.parahaemolyticus。 本研究同時比較細菌自動鑑定儀 Vitek Jr.迷你化細菌鑑定套組Microbact 24E 和EL ISA 之方法，以ELISA 之檢測率99％最高，其他依次為Microbact 24E 之96％...

GUO,MING-YAN, 郭銘宴

碩士 / 國立臺灣大學 / 食品科技研究所 / 79 /

LIN,ZHOU-WEN, 林周文

碩士 / 國立臺灣大學 / 生化科學研究所 / 79 /

WANG, YOU-CI, 王友慈

碩士 / 中國文化大學 / 海洋研究所 / 75 /

HUANG, REN-BIN, 黃仁斌

碩士 / 國立臺灣大學 / 海洋研究所 / 75 /

HUANG, DE-GIAN, 黃德乾

碩士 / 國立海洋大學 / 水產食品科學研究所 / 75 /

SU, MEI-ZHI, 許美智

碩士 / 國立海洋大學 / 水產食品科學研究所 / 75 /

CHEN, GUI-GIU, 陳桂秋

碩士 / 國立中山大學 / 海洋生物研究所 / 75 /

ZHAN, ZHEN-FENG, 詹鎮鋒

碩士 / 國立成功大學 / 礦冶及材料科學研究所 / 75 /

Wang,Shu-Huei, 王琡惠

碩士 / 國立臺灣大學 / 漁業學系 / 81 /

Huang, Shiao-Wei, 黃曉薇

碩士 / 國立臺灣大學 / 動物學系 / 81 / Human pituitary gland tumor cells (Hpt-1) were transfected with a maternal gene from carp egg. A transfectant cell line Hp-3 lost the ability to growth in soft agar. Immunoprecipitation and western blot analysis revealed that in Hp-3 the protein amount of epidermal growth factor receptor (EGFR) was considerably reduced as compared with the parental Hpt-1 cells, on the other hand ...

Yeh,Pei-Yen, 葉培彥

博士 / 國立臺灣大學 / 動物學系 / 81 / A carp maternal cDNA library was transfected into tilapia ovary cells, and on the basis ofcell clonalgrowth rate, a single clone which showed slower growth rate was selected, and a plasmid, de- signated pSC19, was rescued from that clone. Transfection of pSC19 into a human hepatoma cell line showed the transformed cells lost their tumor cell properties, unable to pile -up andgro...