Fu-Hsiung Huang, 黃富雄

碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 / Grouper (Epinephelus coioides) is highly valuable in the aquaculture industry and is amounted to the large types in demand of seafood consumption in Taiwan. Using proteomic approach, this thesis sets two research goals on analyzing the white muscle proteomes of groupers fed by different diets. The first is to understand the biological implications of some differentially expressed proteins, the second is to find proteins with functionality, eventually would help the g...

Zen-Kuei Chang, 張仁魁

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 97 / Cholangiocellular carcinoma (CCA), the second most common primary liver cancer, is associated with a poor prognosis. It has been shown that the molecular mechanism of CCA formation harboring alteration of a number of key regulators for tumorigenesis remains unclear. Here we have generated a zebrafish model that developed CCA with novel gene IMP2 overexpression in liver. We would like to study the function and molecular mechanism of IMP2 in liver tumorigenesis. Tran...

Chi-Huan Lu, 呂笈歡

碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 / The Lutjanidae fish is one of carnivorous coral reef fishes that distributes around the sea area of Taiwan, and it is also the commercially important fish species in Taiwan. Some of large-sized Lutjanidae fish like Etelis carbunculus and E. coruscans, may accumulate high level of vitamin A in the liver and it will cause hypervitaminosis A if ingesting incautiously. In December 2007, one case of hypervitaminosis A was due to ingesting the liver of large-sized Lutjanid...

Yi-Sih Huang, 黃奕絲

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / Viral nervous necrosis disease may cause serious economic threat to grouper culture industry. The disease may cause high mortality（80-100％）particularly in larva and juvenile. Therefore, the control and prevention of this disease is very important. Except improving culture conditions, screening broodstock fish or raising healthy fry. Using vaccine is an effective method to control the disease. The present study used E coli system to express grouper nervous necrosis v...

Chia-Ying Chu, 朱家瑩

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / In this study, the grouper iridovirus of Taiwan (TGIV) was propagated in large numbers, and use of PEG (Polyethylene glycol) for virus concentration and sucrose discontinuous gradient for purification. Then the purified TGIV was used as an antigen to immunize hens by intra-muscular injection. Immunized egg yolks were collected, hydrated and spray-dried to prepare egg powders. The ELISA titers of IgY was found higher than 1?32000 in two boosters and the egg powders ...

Sh-Chien Shu, 許世勤

碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 / Grouper has high economic values in marine food consumption and the aquaculture industry. Especially orange-spotted grouper (Epinephellus coioides) is the major cultured and consumed species in Taiwan. Proteomics was used as a tool to collect the bio-information data of grouper muscles to be utilized in the grouper industry, by which this study begins with grouper skeletal muscle proteome mapping. We select dorsal white muscle and used three methods to fractionate mu...

Yen-Yu Chen, 陳彥妤

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / Two experiments determined the changes of urinary and plasma lysine concentrations by force-feeding the diets (Experiment 1) and caudal vein injection of lysine solution (Experiment 2) to gain more insight into the ability of tilapia(Oreochromis niloticus) to utilize different levels and lysine sources in kidney threshold for urinary lysine excretion. In Experiment 1, after 24 hours fasting, tilapias were force-fed 0.3% body weight of control (fish meal protein) ...

Jr-shiang Yang, 楊智翔

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / Grouper is one of the most important aquacultured fish in Taiwan and disease prevention is a vital issue to grouper culturing as infectious viral diseases had caused great damages in grouper aquaculture. It has been demonstrated that viperin can be induced by type-I/II interferon and viruses, its antiviral activities also be proved. The goal of present study is cloning and characterization of viperin gene in orange-spotted grouper (Epinephelus coioides) and study it...

Hui-Ling Cheng, 鄭惠玲

碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 /

Chien-An Chen, 陳建安

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / In order to investigate relationships between gene defects and human diseases, a recessive embryonic lethal H002 ENU mutant fish showing heart defect was previously obtained by ENU (N-ethyl-N-nitrosourea) treatment of Tg(BMP4:EGFP) transgenic fish. 24 hpf H002 mutant embryos possess cloudy brain. 48 hpf H002 mutants show decreased size of brain with circulation defect, reduced sizes of retinas and total body length. At the same time, some 48 hpf H002 mutants are a...

Tzu-Chieh Hsu, 徐子傑

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / Anemonefish has become one of the hottest species in sea water aquarium in recent years. The study was conducted to determine the optimal dietary protein and lipid levels on growth performance and feed utilization of yellowtail clownfish. In the first trial, white fish meal was used as dietary protein source, cod liver oil was used as dietary lipid source. α-starch and cellulose were used as energy regulater. Yellowtail clownfish (0.69g initial average weight) were ...

Jian-Tong Chen, 陳建同

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / Abstract The experiment was conducted to investigate the effects of dietary supplementation with different carotenoids on skin pigmentation, growth and body carotenoids content of tomato anemonefish, Amphiprion frenatus. We focus on three axes in this study. First, we studied the effect of diets with different astaxanthin level (0、200、400、600、800、1000 or 1200 mg/kg) on 4 months tomato anemonefish (1.36±0.03 g) during 65 days. In the second experiment, diets with...

Wen-Yu Chen, 陳玟妤

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 96 / This study was carried out in two cruises (CR1462 and 1463) from 31-July 2007 to 4-August 2007 on board of the “Ocean Research II”. We investigated the summertime diel assemblages of ichthyoplankton in the Pacific waters northeast of Taiwan. The range of water temperature was from 23.9 to 29.3�aC in the surface at the edge of upwelling, from 28.3 to 30.2�aC in the Kuroshio zone, and from 22.2 to 29.6�aC in the coastal area (I-Lan Bay). Salinity was higher in t...

Yung- Chi Pan Lee, 潘李泳淇

碩士 / 國立臺灣海洋大學 / 海洋法律研究所 / 96 / In 1969, a report by the UN Economic Commission for Asia and the Far East (ECAFE) indicated the possibility of large oil reserves on the continental shelves of the Diaoyutai Archipelago. The report has raised attention from Japan, Taiwan and China. In May 1970, the Japanese government attempted to assume ownership on Diaoyutai Archipelago, which belonged to Taiwan. The government of Taiwan then made a serious statement to express the firm resolve of the country to ...

Yu-Sheng Tsai, 蔡宇昇

碩士 / 國立臺灣海洋大學 / 河海工程學系 / 96 / The precipitation is unevenly distributed over time and space in Taiwan. It leads to frequent floods and droughts. Constrained by Taiwan，s topography and rainfall distribution ,the water-resource management is difficult and needs to be thoroughly planned to cope with rising water demands. In this research, an early warning system was developed and proposed for drought management on the real-time reservoir operation. The system consists of three essential element...

Yun-Sheng Hsieh, 謝韻聲

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 /

Sian-Tai Liu, 劉咸台

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / Abstract Brain is a highly energy consuming organ and sensitive to energy perturbations. Previous studies have found that environmental factors would induce changes in brain intracellular homeostasis and energy parameters. In recent studies, fish appeared to use lactate as an energy substrate during acclimation and exposure to severe environment changes. Nevertheless, the mechanism of the lactate transportation in brain during acclimation in low temperature is...

Yuan Chih Lin, 林淵智

博士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 96 / The sounds of male big-snout croaker, Johnius macrorhynus, produced under hand-held and voluntary conditions (in large aquarium and in the field) were compared. Voluntary calls included “purr” and “dual-knocks”; only purrs were produced when the fish was hand-held. The purr is composed of pulses in which the first inter-pulse interval was 6-9 times longer than the other inter-pulse intervals. Purrs emitted under these conditions did not differ significantly, su...

Yen-Hua Chen, 陳彥樺

博士 / 國立臺灣海洋大學 / 食品科學系 / 96 / Summary Zinc concentration in the digestive tract of common carp is always 3-10 times higher than most animal tissues. It was known that this high zinc came from a 43 kDa zinc-binding membrane protein. An indirect immunoperoxidase staining method using antibody against the 43 kDa zinc-binding protein was applied to the sections of digestive tract of the fish. It was found that the zinc-binding protein is mainly located in the connective tissue of its lamina propri...

Wen Ting Sung, 宋文汀

碩士 / 東海大學 / 生物學系 / 88 / Respiration and ion regulation are the two principal functions of teleostean gills. Mitochondria-rich cells (MR cells; previously known as chloride cells) mainly distribute in the gill filaments of fish. The MR cells proliferate to increase the ionoregulatory capacity of the gill in response to osmotic challenges. Gill lamellae mainly consist of pavement cells, which are the major site of gas exchange. Some species also have MR cells in the gill lamellae. This will greatl...

Chia-Jen Shieh, 謝嘉珍

碩士 / 國立臺灣大學 / 漁業科學研究所 / 88 / The decapeptide gonadotropin-releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. In order to know the important role of GnRH in fish reproductive physiology, the expression levels of GnRH mRNA in brain and ovary of Mozambique tilapia, Oreochromis mossambicus were observed. Previous studies using reverse transcription- polymerase chain reaction (RT-PCR) and Southern blot analysis have demonstrated that there are cGnRH-II, sbGnRH and s...

Li-Fen Lee, 李莉芬

碩士 / 國立臺灣大學 / 漁業科學研究所 / 88 / Overexpression of v-ski, or its proton-oncogene, c-ski, has an effect on proliferation, morphological transformation and myogenic differentiation in fibroblast culture and transgenic animal. As a transcription factor, the biological role of c-Ski depends on the cellular environment and is under deciphering. Two types of c-ski, tski1 and tski2, have been identified in tilapia, Oreochromis aurea. tski transcripts are widely expressed in different adult tissu...

Chien-Ho Liu, 劉健合

碩士 / 國立臺灣大學 / 動物學研究所 / 88 /

Li Chia-Chi, 李佳琪

碩士 / 國立海洋大學 / 漁業經濟研究所 / 88 /

Chin-Shen Chen, 陳進生

碩士 / 國立海洋大學 / 漁業科學學系 / 88 / Taxonmically, Rajiformes is included in subclass Elasmobranchii under class Chondrichthyes, being divided into 4 suborders and 12 families. The phylogenetic relationships within rajiformes are still in vague. In order to infer their phylogenetic relationships, a molecular approach with mtDNA 12S rRNA sequencing of 12 families and 33 species of rajiformes, chimaeras, and sharks, were analyzed. The results suggest that Rajiformes is not a monophyletic group. Subclass El...

Shyh-Jiun Michael Rau, 饒世君

碩士 / 國立海洋大學 / 海洋生物研究所 / 88 /

yeh-yen-hung, 葉彥宏

碩士 / 國立海洋大學 / 食品科學系 / 88 / To elucidate the responsible toxic components in the bile juice of grass carp, the bile alcohol salt from grass carp bile juice was purified and identified. By IR、1H-NMR, 13C-NMR and MS spectrometry analyses, a compound with molecular formula of C27H48SO8 and molecular weight of 532 was obtained from grass carp bile juice and identified as 5α-cyprinol sulfate. In the meantime, the determination method for bile acids in fish bile juices was developed. It ...

Yu-Jen Chein, 簡佑任

碩士 / 國立海洋大學 / 食品科學系 / 88 / Place the “Katsuobushi” at 25oC, RH 80% for a month; isolate the strain that can hydrolyze olive oil by olive oil agar. There are 18 strains that can producing lipase, strain S03、S04、S05、S06 and S16 has better ability to producing lipase that can form blue rings which diameter is 3.0、2.5、2.5、3.0 and 2.8 cm after cultivated in olive oil agar plate at 25oC for 72 hours. Use soybean oil agar plate to test the ability of hydrolyze soybean oil, strain S03、S04、S05、S06 and S1...

Hsin-I, Hsiao, 蕭心怡

碩士 / 國立海洋大學 / 食品科學系 / 88 / Abstract The Investigation was conducted to figure out the causes that Lactococcus lactis subsp. lactis CCRC 14016 can inhibit the formation of histamine in mackerel fillets. Firstly, series of storage test of treated fillets were carried out. Mackerel fillets were treated by soaking in L. lactis subsp. lactis CCRC 14016 cell suspension containing 3﹪salt for 24 hr, and then by blow-drying for 8 hr and finally by storing for 20 days. All the treatment and ...

朱永桐

碩士 / 國立臺灣大學 / 漁業科學研究所 / 87 / Fish were able to adapt to the fluctuating environment by behavioral responses and physiological regulations. The groupers, euryhaline teleosts, were tolerable and survival to the wide salinity range, from 11 to 41 ppt. The approach of salinity shock was employed in this study to comprehend the physiological responses of the groupers, Epinephelus coioides, and specially focused on osmoregulation and carbohydrate metabolism. The acclimated groupers at t...

Hsiao-Chen Lin, 林曉珍

碩士 / 國立臺灣大學 / 漁業科學研究所 / 87 / Tilapia, an economically important cultured teleost in Taiwan, has played a significant role in the fisheries development in the past. As a tropical species, they are suitable for culture in the warm waters. However, mass mortality of these cultured tilapia resulted from less tolerable attributes of this species to the cold, has often caused tremendous loss for the tilapia culture industry, when the severe cold current approaches in the winter season. This study is t...

Ching Pei Chen, 陳金貝

碩士 / 國立臺灣大學 / 漁業科學研究所 / 87 / In gill epithelium, mitochondria-rich cells (MR cells) are considered to be the major cell type for ion- and osmo- regulation. In fresh water, gill MR cells are suggested to be responsible for ion uptake. Based on SEM (scanning electron microscope) observations on the apical surface, MR cells are classified into three types, wavy-convex, shallow-basin, and deep-hole types. Fish were acclimated to three hypotonic medium, F-Cl (chloride-free)、H-Na (high Na2SO4) and H-N...

Hsin-Hui Feng, 馮馨慧

碩士 / 國立臺灣大學 / 漁業科學研究所 / 87 / Using rapid amplification of cDNA ends (RACE), full length cDNAs of Na,K-ATPase α1 and α3 subunits of tilapia (Oreochromis mossambicus) were cloned and sequenced. Clone TG33 is 3390 bp in length and encodes a polypeptide of 1023 amino acids, while clone TH3 is 3581 bp in length and encodes a polypeptide of 1010 amino acids. Clones TG33 and TH3 showed 91 ％ and 88 ％ identity at amino acid level with previously described animal Na,K-ATPase α1 and α3 subunits, respective...

羅婉瑜

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / The objective of the study were to investigate the regulation of corticosteriods and sex steriods on the phagocytosis in tilapia and common carp. The peripherial blood leucocytes culture system was developed for the phagocytosis experiment. The study was divided into three parts, 1) phagocytosis in vitro in tilapia, 2) phagocytosis in vivo in tilapia, 3) phagocytosis in vitro in common carp. 　　Five does (1pM, 0.1nM, 10nM, 1μM及0.1mM/well) of corticosteroids and sex s...

Wan -Jane Chou, 邱琬貞

碩士 / 國立海洋大學 / 水產生物技術研究所 / 87 / Abstract A strong spontaneous expression of heat shock protein 70 (HSP 70) was consistently detected in the extraxt of Zebrafish (Danio rerio) at early larval stage (84 h after fertilization), while HSP 70 expression was either at low level or barely detectable in Zebrafish at earlier or later developmental stage. The spontaneously expressed HSP 70 was not recognized by a McAb against HSC 70, suggesting that it may be induced by growth or differentiat...

Dar Yi Wang, 王達益

博士 / 國立海洋大學 / 漁業科學學系 / 87 / In order to investigate the toxic effects of tributyltin (TBT) on marine teleosts, the gill and sensory tissue (vision, olfactory and gustatory) of thornfish (Terapon jarbua Forsskal) were obtained to study the physiological and behavioural changes. 1. Toxic effects of TBT on gill and cornea The gill epithelial cells showed swelling and necrosis after exposure of TBT (10, 50 and 100 mg/l) for 7 d, the oxygen consumption also significantly inc...

黃子欣

碩士 / 國立海洋大學 / 食品科學系 / 87 / In general, the dried squid was consumed after rehydration or roasting. According to suggestion of customer, there were two defects. The first, the texture of conventional soften squid with alkali treatment during rehydration will be losing its original flavor; the second, the roasting dried squid had character flavor, but its texture was too tough to unaccept. In order to improve the texture of dried squid mantle, the squid was treated with different concentration of ...

WeiMing Su, 蘇偉銘

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / The present study is aimed to (1) investigate the interactions between extracellular products (ECP) of pathogenic vibrios and serum components of grouper ; (2) partially purify the protease inhibitor in the grouper serum and (3) discuss its possible role on vibriosis. Grouper serum contains one protease inhibitor which can inhibit ECP protease activity of Vibrio alginolyticus. It could inhibit activity of serine protease but not that of cysteine protease. T...

Wan Yu Lo, 羅琬瑜

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / The objectives of the study were to investigate the regulation of corticosteriods and sex steriods on the phagocytosis in tilapia and common carp. The peripherial blood leucocytes culture system was developed for the phagocytosis experiment. The study was divided into three parts, 1) phagocytosis in vitro in tilapia, 2) phagocytosis in vivo in tilapia, 3) phagocytosis in vitro in common carp. Five doses (1pM、0.1nM、10nM、1μM及0.1mM / well) of corticosteroids ...

鄭靜憶

碩士 / 國立海洋大學 / 水產養殖學系 / 87 /

Chiung-Yu Huang, 黃炯毓

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / Fishes appear to utilize dietary carbohydrate poorly. It has been shown that carbohydrate is generally the major dietary source of energy in most domestic animals, however, it is considered to be of limited value in the nutrition of fish. Differences in carbohydrate digestion、utilization、absorption and metabolism between fish and mammals the major reasons. Causing dietary differences between these two animal groups. The objective of this experiment was to observe the ...

Jiun - Woei Chang, 張君瑋

碩士 / 國立海洋大學 / 水產養殖學系 / 87 /

Lin Ming-Hong, 林明宏

碩士 / 國立清華大學 / 生命科學系 / 89 / Ligand-gated ionotropic glutamate receptors mediate a majority of excitatory synaptic transmission in the CNS and are involved in neuronal development, excitatoxicity, and synaptic plasticity. Previous studies have revealed that protein-protein interactions with the C-terminal domains of glutamate receptor subunits are involved in the modulation and clustering of receptor function at excitatory synapse. So far, mammalian studies have shown that the AMPA receptor subu...

Yu-Li Chen, 陳悠里

碩士 / 國立中山大學 / 海洋生物研究所 / 89 / This research determined the in vitro, intraperitoneal injection and dietary immunostimulatory effects on the grouper Epinephelus malabaricus of chitosan and its derivatives with different molecular weight, chitosan, polyglucosamine and N-acetyl-chitooligosaccharides. Respiratory burst activity of head-kidney phagocytes isolated from the grouper incubated in vitro with the chitosans at a range of concentrations was studied. Respiratory burst activity generally decrea...

Yu-Hsia Wu, 吳玉霞

碩士 / 國立中山大學 / 海洋生物研究所 / 89 / This research determined the influence of dietary vitamin E levels on the growth, body composition and various health indices of juvenile Epinephelus malabaricus. Five experimental diets were formulated by adding all-rac-a-tocopherol in 0, 200, 400, 1000 and 3000mg/kg feed to the basal feed that used casein as the sole protein source. The juveniles, having an average weight of 30g, were cultivated in an indoor closed recirculation system, with 6 fishes in each tank, ...

Wang, Chu-Hui, 王琡惠

碩士 / 國立臺灣大學 / 漁業科學研究所 / 81 /

Yuan, You-Gang, 袁又罡

博士 / 國立臺灣大學 / 動物學研究所 / 82 /

Che-Yu Li, 李哲宇

碩士 / 國立臺灣師範大學 / 化學系 / 100 / Fluorescent nanodiamonds (FNDs) have recently developed into an exciting new tool for bioimaging applications. The material possesses several unique features including high biocompatibility, easy bioconjugation, and perfect photostability, making it a promising optical nanoprobe both in vitro and in vivo. This work explores the potential application of the novel nanomaterial as a photostable, nontoxic tracer in vivo using zebrafish as a model organism. We introduce...

Ming-Chong Ng, 吳民聰

博士 / 國立臺灣師範大學 / 生命科學研究所 / 100 / In teleost fishes, the lateral (Dl) and medial (Dm) division of the dorsal telencephalon are important in learning and memory formation. Tract-tracing studies revealed that neural connections are formed between these regions via afferent Dl fibers projecting to the Dm. However, research analyzing Dl–Dm synaptic transmission is scant. Ray-finned zebrafish has been a widely used model organism in behavioral research such as addiction, anxiety, and in learning and me...

Chu, Chun-I, 朱駿逸

碩士 / 國立清華大學 / 動力機械工程學系 / 102 / 因申請專利緣故，資料延後公開