Han-KaiJiang, 江翰鍇

碩士 / 國立成功大學 / 生物科技研究所 / 102 / In recent years, nervous necrosis virus (NNV) and Iridovirus infection caused high mortality and serious losses in Taiwan grouper industry. Therefore, the development of the multivalent recombinant subunit vaccines would be an ideal strategy for disease control and prevention. Firstly, to evaluate the efficacy of vaccine, we determine the half lethal dose of NNV and Iridovirus on giant grouper (Epinephelus lanceolatus). The results showed that the LD50 in NNV was 10...

Yao-TingChang, 張耀庭

碩士 / 國立成功大學 / 工程科學系碩士在職專班 / 102 / The goal of this study is to investigate the effect of light intensity, water recirculating system and nutrient concentration on the growth of Chinese cabbage in an aquaponic system. The hardware architecture is designed based on the hydroponic cultivation technique which is applied in the aquaponics system of three irrigation water recirculating systems, including water cycle, overflow and ebb-and-flow systems. Moreover, white-light-emitting diodes are used to ...

Pei-Chun Hsu, 許培群

碩士 / 中國醫藥大學 / 營養學系碩士班 / 100 / Houttuynia cordata Thunb is a medicinal plant used in traditional Chinese medicine. The major aim of present study was to examine the effects of Houttuynia cordata Thunb aqueous extract (HCE) in mice treated by high fat diet. Butter at 15% (w/w) was used to prepare high fat diet. C57B6 male mice were divided into five groups: (1) normal group (normal diet and water); (2) high dose HCE group (normal diet and 8% of HCE); (3) high fat diet group (15% butter and water)...

Po- Rung Jiang, 江柏蓉

碩士 / 國立彰化師範大學 / 生物技術研究所 / 101 / Thioacetamide (TAA) is a hepatotoxin that can cause hepatocellular carcinoma in mice, but little is known about its toxic effect on embryonic development. In the previous study, we treated zebrafish embryos with 0-32 mM TAA for 0-72 h, the survival rate were decreased to about 80% and the edema in pericardial sac (eps) were shown. For understanding the mechanism about TAA teratogenesis, we used DNA microarray to analyze the gene expression in zebrafish embryos whe...

Meng-Ru Li, 李孟儒

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 101 / Two feeding trials were conducted to evaluate the dietary zinc (Zn) requirement for the optimal growth of hybrid tilapia with casein or soybean meal (containing 1.95% endogenous phytic acid) as a source of protein . Experiment 1: Casein was the major protein source. Diets supplemented 12 g/kg sodium phytate (phytic acid contented 10 g/kg). Hybrid tilapia juveniles of approximate 0.93 g body weight were fed casein based diets supplemented with 0, 30, 60, 90, 120, ...

Zhang Feng-Ming, 張峯銘

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 101 / The present study found that 96 h-LC50 of Cd was 100 μM and 80.3 μM on goldfish and common carp, respectively. The objective of this study was to clarity what are the possible reasons of the Cd-resistance different between the goldfish (Carassius auratus) and the Common carp (Cyprinus carpio). Our results showed that: (1) MT contents of goldfish was 2.1 fold higher than the carp before Cd exposure, and the goldfish induced MT expression were 1.9, 4.4 and 1.0 fold...

Siou-LianHu, 胡秀蓮

碩士 / 國立宜蘭大學 / 生物技術研究所碩士班 / 101 / Grouper iridovirus (GIV), belonging to the Ranavirus genus of the Iridoviridae family. GIV is one of the most important viral pathogens of grouper, particularly at the fry and fingerling stages and causing heavy economic losses. In this study, the cloning, characterization, and expression analysis of ORF055L, ORF056R, and ORF076L of GIV were investigated.The ORF055L is 1128 bp and encode a putative protein of 376 amino acids with a predicted molecular mass of 41....

Jing-Jie Shen, 沈靖傑

碩士 / 淡江大學 / 物理學系碩士班 / 101 / Magnetic nanomaterial has extensive potential for many applications, such as magnetic recording media, localize heating treatment, and drug delivery systems for pharmaceutical cure in vivo. However, the toxicology and biocompatibility of magnetic nanoparticles(NPs) should be studied. In this work, the security and impact of magnetic NPs, iron oxide, were investigated by injecting and feeding iron oxide NPs into zebrafish embryos and adult zebrafish to check the etholo...

Juan, Fu Kuan, 阮甫寬

碩士 / 國立政治大學 / 外交學系戰略與國際事務碩士在職專班 / 101 / ADIZ (Air defense identification zone) is setting up for the purpose of ensuring the national security, to grasp the origin and attempting of aircraft near its territory. As for the Diaoyutais, an island group that is part of Taiwan, it becomes a focus among mainland China, Taiwan and Japan, as a territorial dispute, owing to series factors in history. Based on the trend of international reality, this study would try to analyse the issue of ADIZ among main...

江盛宗

碩士 / 國立政治大學 / 國家安全與大陸研究碩士在職專班 / 101

Tsung-wei Lin, 林宗威

碩士 / 國立中山大學 / 海洋生物研究所 / 100 / As one of the major preys of many important economic fish species such as swordfish and dolphinfish in waters off estern Taiwan, flyingfish belongs to low-end consumers in the food chain with the function of maintaining the stability of the Kuroshio marine ecosystem. Hirundichthys oxycephalus is the primary component of flyingfish-egg fishery captures in the northeastern waters of Taiwan, and is also one of the dominant species of flyingfish in eastern waters of Tai...

Shih-Ting Wu, 吳詩婷

碩士 / 國立中山大學 / 海洋生物研究所 / 100 / This research studied the dietary effect of arachidonic acid (ARA) on growth performance and immune responses in juvenile grouper (Epinephelus coioides). The test diets used casein as protein source, and contained 9 g/100g lipid. Cod liver oil and arachidonic acid oil were used to adjust the ARA levels to 0, 0.2, 0.4, 0.6, 0.6, 0.8, and 1.0 g/100 g diet in the 5 test diets, respectively. Result of 12-week feeding trial show that the ARA supplementation had no signif...

Bing-Hsien Liu, 劉柄顯

碩士 / 國立體育大學 / 休閒產業經營學系碩士班 / 101 / The researcher found that teaching and learning front craw stroke (freestyle) is hard to changing breath, because with too much strength and not efficacy and unbalance body while swimming. Terry Laughlin invested the total Immersion (Fishlike) Swimming Education Method (TIFSEM). After a long term observed on teachers and students’ front craw stroke (freestyle) teaching and learning process, TIFSEM had a better improvement. Although TIFSEM does improve most o...

Shih-Feng Pan, 潘世鋒

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 101 / Multicellular organisms to eliminate unneeded and damaged cells in a process by apoptosis. When the organism to external stimuli, such as UV irradiation or drug-induced, resulting in cell DNA damage. Organism will initiate apoptosis to clear damaged cells. In addition, apoptosis during embryonic development are also involved in the pattern of individual organisms sculpture, and new cells and apoptotic cells must be in equilibrium in the organism. Many diseases hav...

Shih-Hao Tu, 涂士豪

碩士 / 國立臺灣海洋大學 / 食品科學系 / 101 / Farmed grouper is a high economic value species, the yield of farmed grouper is accounting for 95% of total fishery production in Taiwan. Fresh raw grouper is chopped as fish steak, and its appearance features might be removed, making difficult in identifying the species. The DNA-based method for the identification of grouper species has been established, but distinguished by protein method is still lack. This study is to identify farming grouper species by protein-...

Hong-Han Chen, 陳弘瀚

碩士 / 國立臺灣海洋大學 / 食品科學系 / 101 / Abstract This study investigated the effect of air exposure on the proliferation of erythroid cells in the head kidney and blood of common carp. In order to produce a controlable stress conditions, we exposed the fish to air for 2 h then them returned to the aquarium for another 2 h. This process was repeated one to six times. The common carp experienced to different stressed degree, i.e., control (resting fish), A1, A2, A3, A4, A5, A6 (air exposure 2h ×1, ×2, ×3, ...

Yu-Chen Lin, 林侑臻

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 101 / The development of grouper culture in Taiwan has been threatened by the infection of nervous necrosis virus (NNV) and caused significant loss especially during the larvae stage. For the disease control and prevention study of NNV, the mechanism of virus infection and host response has gradually understood and developed in recent years. In this experiment, we stimulated the innate immune responses in vivo as prevention the invasion of NNV. Since using herbal extract...

Yu-Keng Lin, 林鈺庚

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 101

Wei-Yi Chen, 陳瑋毅

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 100 / Basic-helix-loop-helix (bHLH) transcription factor plays an important role in the process of vertebrate embryonic development, and the hypoxia -inducible factors (HIFs) play a key role to adapt physiological functions during hypoxic stress. NeuroD is a basic helix loop helix transcription factor that is widely expressed in CNS and neuronal cells. NeuroD regulates cell cycle and control neuronal differentiation. Knock down hif2α diminished survivin (birc5a and birc...

Shih-Han Wen, 溫士漢

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 100 / Recent study revealed that depletion of hypoxia induced factor 1 alpha (hif1α) expression resulted in decrease of GATA binding protein 1 (gata1) transcription, suggesting that hif1α controls gata1 expression. Similar result was also found in humen erythrocyte. Blocking gata1 expression converted hematopoietic stem cell (HSCs) differentiation from gata1-mediated erythropoiesis to spi1-mediated myelopoiesis. There is an antagonistic regulation between gata1 and spi1...

Yi-FanFang, 方苡帆

碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 101 / Innate immune system is important for the disease resistance of fish. The innate immunity of fish is divided in three parts: physical barriers, humoral and cellular components, which are common to resistance variety water-pathogen infection and maintain the healthy statement of fish. Interleukin-6 (IL-6) is a cytokine of the lymphocytes hormone (lymphokine), mainly induce lymphocytes to reaction. It plays important roles in the innate and acquired immunity. In t...

Yung-ChingChu, 朱永靖

碩士 / 國立成功大學 / 機械工程學系碩博士班 / 101 / The effects of combination of straight channels with staggered herringbone grooves and curved channels on the performance of micromixers are investigated by numerical simulations. The validity of numerical simulations is examined by comparing the numerical and experimental results. There are to mechanisms for mixing enhancement in the proposed micromixers. One is the stretch and fold of fluid interface induced by staggered herringbone grooves and the other is th...

Fang-Chi Kang, 康芳齊

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 100 / Bactericidal effect of organic acids (acetic acid, lactic acid, tartaric acid and citric acid) on histamie-producing bacteria was studied. Minimal bactericidal concentrations (MBCs) of organic acids and preservative against histamie-producing bacteria for 24 hour were as followed : 1% acetic acid and tartaric acid, 0.5% lactic acid and 3% citric acid for Enterobacter aerogenes; 1% acetic acid, 0.5% lactic acid, 3% tartaric acid, >3% citric acid for Morganella ...

Chao, Chih-Yun, 趙芷妘

碩士 / 國立宜蘭大學 / 生物技術研究所碩士班 / 100 / Aging refers to a set of processes that lead over time to a gradual increase in vulnerability to be damaged and the probability of death. Fish is a promising model animal for aging research. It is well known that ayu (Plecoglossus altivelis), a common freshwater fish of East Asia, die after spawning and have a life span of only 1 year. The determinants for such a short life span are still ambiguous, but are probably connected with spawning. In the present study, ...

Liou, Jiunyi, 劉俊毅

碩士 / 國立宜蘭大學 / 食品科學系碩士班 / 100 / In this study immature kumquat was extracted by hot water, ethanol, and ethyl acetate. The obtained extracts were added in oil of mackerel, separately, to evaluate the inhibitory activity on lipid oxidation under heating condition. In order to confirm the effective compounds, a semi-preparative C18 column was used to separate and fractionate the target substances. Furthermore, the inhibitory model of antioxidant and identification of the effective compounds were al...

Lee, Sihyi, 李思怡

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 100 / The hepatocyte nuclear factors-3 (HNF-3) family members HNF-3α, HNF-3β and HNF-3γ are hepatocyte-enriched transcription factors, they play important roles in controlling development, differentiation, metabolism and organogenesis. In our previous study, the expression of insulin-like growth factor-I/II (IGF-I/II), HNF-1α, -1β and -3β were detected in the liver and gonads of tilapia, and expreession level of HNF-3β was higher than others and it could be regulated b...

Yi-chen Huang, 黃一誠

碩士 / 國立成功大學 / 地球科學系碩博士班 / 97 / High pressure and ultrahigh pressure metamorphic rocks occur in Yuka, northern Qaidam basin, northwestern China. Many differences in petrography between eclogite in eastern Yuka and western Yuka observed, such as microstructure, grain size, and mineral composition. According to the observation of petrography and mineral composiotion, four metamorphic stages of eclogite in eastern Yuka are recogrized: the stage Ⅰ is epidote - amphibolite facies , the mineral assem...

Reng-Long Yang, 楊人龍

碩士 / 國立成功大學 / 化學系碩博士班 / 96 / Collagens (acid-solubilized collagen, ASC；pepsin-solubilized collagen, PSC) , gelatin and collagen hydolysate were prepared from hybrid tilapia skin and partially characterized. The yield of collagen was improved due to the addition of pepsin in the purification process. Only ASC and PSC possessed a triple helical conformation. Under low temperature condition, the digestion of collagen by pepsin improves its water solubility. On the other hand, collagens exhibited ma...

Po-Shuo Chiu, 邱柏碩

碩士 / 國立臺灣科技大學 / 管理學院MBA / 103 / This paper studies the issues from small and medium enterprises transfer to internationalization in the growth process. The company of this case established in 1977, is a typical small enterprise, which focus on manufacturing different kinds of fuse. The chairman has technical background and with his sustained efforts, this company so as to hold a place in the fuse filed in Taiwan. After the year 2000, when the manufacturers in Taiwan emulatively invested factorie...

Chung-Hsiang Yuan, 袁崇翔

碩士 / 國立臺灣大學 / 漁業科學研究所 / 95 / Following infection, viruses use different mechanisms to control host cell’s genes expression. Some of the host genes may be activated or suppressed. And its makes the host cell to be an appropriate place for virus to replicate and assemble. Recently, viral nervous necrosis disease causes a great damage to grouper aquaculture in Taiwan. The pathogen of this disease has been identified as nervous necrosis virus (NNV). In order to understand the infection characteristi...

Yu-Kai Huang, 黃昱愷

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 95 / Amiodarone is a potent anti-arrhythmic drug and widely used in clinic, but it is classified as FDA category D. Some adverse effects to the fetus have been reported with amiodarone, including fetal hypothyroidism, low birth weight and bradycardia. However, amiodarone is still used in case other agents are failure treatment and the benefit from using this drug in pregnant women outweighs the fetal risk. To develop an animal model for studying the drug effects on em...

Chuan-Yang Fu, 傅傳揚

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 95 / Troponin I (TnnI), a constituent of the troponin complex on the thin filament, provides a calcium-sensitive switch for striated muscle contraction. Cardiac TnnI is a highly sensitive and specific marker of myocardial injury in the acute coronary syndromes. Although TnnI gene has been identified in birds and mammals as it encodes the isoforms expressed in cardiac muscle, fast skeletal muscle and slow skeletal muscle, there is extremely little known about the devel...

Sheng-Yi Chuang, 莊聖儀

碩士 / 國立臺灣大學 / 漁業科學研究所 / 95 / The conservation of primary freshwater fish is strongly related to the continuation of species diversity. Such an issue has often been overlooked in Taiwan over the years. However, with the awakening of environmental proteciton consciousness amd the developing of related laws, the way on how to conserve the primary freshwater fish had become an important academic topic. The purposes of this study are to discuss the relationship between the distribution of prima...

Wei-Ta Chen, 陳威達

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 95 / six1 gene belongs to the sine oculis/six gene family that plays important roles in morphogenesis, organogenesis, and cell differentiation. Studies in Drosophila have revealed that six family, eyeless (pax family), eyes absent (eya family) and dachshund (dach family) form a synergistic regulatory network to trigger compound eye organogenesis. six1 knockout mice showed that knockdown of six1 causes extensive muscle hypoplasia which affects most of the trunk muscles...

許殷瑋

碩士 / 國立臺灣師範大學 / 地球科學系 / 95 /

Yu-Da Zheng, 鄭育達

碩士 / 國立嘉義大學 / 水產生物學系研究所 / 95 / Metallothionein（MT） was first found in equine kidney. Up to the present, it has been investigated around for 50 years. In teleost, the physiological functions of MTs hasn’t been completely cleared. The past studies, most were almost focused on metallothionein 2（MT-2）and fewer studies on similar to metallothionein B（sMT-B） research in zebrafish .The physiological functions of MT-2 and sMT-B of zebrafish were compared after challenged with cadmium and cold shock. Dur...

Shao-Ju Weng, 翁紹儒

碩士 / 國立嘉義大學 / 水產生物學系研究所 / 95 / Two types of mullet, the migratory (M-type) type with Pgi-A100 and resident (R-type) with Pgi-A135, live in different temperature environments around the coastal and estuaries of Taiwan. In this study, we experimented on mullet with 3 genotypes, Pgi-A 100/100, 100/135 and 135/135, and quantifying expressive differences of HSP70 to infer the physiological response. We can go a step further to identify two group of resident type and one migrant type using mtDNA 16s...

Yen-Chun Chen, 陳讌君

碩士 / 國立宜蘭大學 / 生物技術研究所碩士班 / 96 / This study demonstares the induction of apoptosis in BM (barramundi muscle) and BSB (barramundi swim bladder) cell lines, by grouper iridovirus (GIV) infection. The apoptosis was detected and confirmed through various methods. At MOI of 10, cytopathic effect (CPE) characterized as cell shrinkage and rounding was first observed in both GIV-infected BM and BSB cells at as early as 45 min pi (post-infection). GIV infection appeared to progress more rapidly in BM cell...

Chia-Nan Chien, 簡嘉男

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 96 / In rapid growth of farming industry, many dealers are still managing in such a traditional way for home-farmer style that it is easily impacted by production and marketing unbalance. In order to improve management efficiency of perch farming industry and corresponding strategy, this study research production and marketing of perch farming industry and investigate SWOT analysis with external and internal factors. Furthermore, a precaution strategy has also proposed...

You-Jiun Liu, 劉祐浚

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 96 / This research in made during the period from June to August, 2007. The researcher collected fish specimen, pond water, sediment and fish feed in Sindian village, Dongshih Township and Budi Town of Chiayi County. Moreover, the testing data of those samples was analyzed for their concentration of copper, zinc, lead and cadmium. The result was used for ecological risk assessment in Aquaculture environment and risk assessment in consumers’ health. The research method ...

Yu-Hsuan Kai, 蓋玉軒

碩士 / 國立臺灣大學 / 動物學研究研究所 / 93 / Nervous necrosis virus (NNV) is the most economically important pathogen of cultured groupers in Taiwan causing mass mortality 80~100% in fry and larvae. The aim of this study is to develop inactivated NNV vaccine, and a method of treating or preventing VNN through immersion immunization of grouper fry. The culture supernatant of NNV-infected GF-1 cells was collected after cytopathic effect (CPE) complete, and used for the preparation of inactivated vaccine and for ...

Li-Ling Huang, 黃麗玲

碩士 / 國立臺灣大學 / 動物學研究研究所 / 93 / Nervous Necrosis Virus（NNV）can induce acute infection and mass mortality of grouper fry and larva. The aim of this study is to detect and analysis of the differentially expressed grouper genes after NNV infection. By suppression subtractive hybridization (SSH) and Northern analysis, two cDNA sequences（FB2, FB9） were selected by forward subtraction and two cDNA sequences（R1H1, R2H12）were selected by reverse subtraction. These four cDNA sequences were analyzed by Blas...

Chang-Hung Wu, 吳昌宏

碩士 / 國立臺灣大學 / 生物產業機電工程學研究所 / 93 / The objectives of this research are to improve the design and performance of a TEC cryomicroscope, and to perform experiments on the observation of IIF behavior of zebrafish embryos. The cryomicroscope system includes a cryostage, a microscope with image grabbing system, a current amplifying circuit, and A/D signal processing system. The improvement mainly focused on the design of the cryostage. By using a glass well containing low freezing point liquid such as ...

Yi-Tsun Lee, 李依純

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 93 / Septin is a highly conserved group of cytoskeleton protein that have been found in almost all eukaryotes but not in protozoa or plants. The septin genes were originally identified in Saccharomyces cerevisiae, and have an important role during cytokinesis. The budding yeast mutants that exhibited defects in septins will produce abnormal morphology during the budding process. Recent researches implicate that different Septins have diverse cellular roles including c...

Shin-Jie Huang, 黃信傑

碩士 / 國立臺灣大學 / 漁業科學研究所 / 93 / Mouse double minute-2 (mdm2) gene has been shown to be overexpressed in many cancers that presumably lead to the inactivation of tumor suppressor protein p53. In addition,MDM2 also has p53-independent functions and plays significant roles in cell cycle control, differentiation, cell fate determination, DNA repair, basal transcription, and other processes. Moreover, MDM2 overexpression has been demonstrated to have growth arrest function in different animal model such...

Chien-Ju Huang, 黃倩茹

碩士 / 輔仁大學 / 大眾傳播學研究所 / 94 /

Chia-Hsin Li, 李嘉馨

碩士 / 大葉大學 / 生物產業科技學系 / 94 / Live tilapia (Orechromis niloticus) immediately killed were used as raw materials. Two types round fish and crushed meat prepared after three pieces filleting and crushing of the round fish were stored in a 4℃ refrigerator equipped with electrostatic field induced device (EFID, E-group) to investigate the changes in freshness and quality. Storage in a common refrigerator controlled at the same temperature was used as the contrast (R-group). The results obtained were ...

駱明瑜

碩士 / 國立清華大學 / 分子與細胞生物研究所 / 94 / L-glutamate is the major excitatory neurotransmitter in the vertebrate central nervous system. Ionotropic glutamate receptors are divided into three subtypes, AMPA-, kainate- and NMDA-preferring receptors. Oreochomis mossambicus (tilapia) expresses eight AMPA receptor subunits (fGluR1~4a and fGluR1~4b). The glutamate-activated currents of fGluR1a(i), i represents the flip form, were only detected in the presence of the desensitization blocker, cyclothiazide. T...

Ming-Chang Ou, 歐明昌

碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 94 / In recent years, viral nervous necrosis disease （VNN）is the most acute disease of hatchery-reared grouper in Taiwan . The causative agent of VNN was nervous necrosis virus （NNV）and the mortality of grouper larvae and juvenile during disease outbreak were usually above 90％. NNV belongs to the beta subgroup of the virus family nodavirade. Nodavirade has another subgroup alpha-nodavirus which infect inscets and plants. From the study of alpha-nodavirus, it is known ...

Wen-Ni Chang, 張文妮

碩士 / 國立成功大學 / 醫學檢驗生物技術學系 / 94 / Serine hydroxymethyltransferase (SHMT, EC 2.1.2.1) mediates the principle pathway for incorporation of one-carbon units into cells by reversibly converting serine and H4PteGlu to glycine and 5,10-CH2-H4PteGlu which is required for biosynthesis of many important molecules including nucleotides, amino acid, neurotransmitters and S-adenosyl methionine. The level of SHMT is also found to be elevated in tumor cells. In higher organisms more than one SHMT isoforms are...