Yi-Chun Lai, 賴怡君

碩士 / 國立中興大學 / 農村規劃研究所 / 96 / On September 21/1999, the Chi-Chi earthquake caused loss of over 2000 lives and disastrous damages to properties, facilities including houses, roads, and bridges for many cities and rural communities in central Taiwan. Se-Shui community in the Da-Yen Village is one of these who suffered the most severe detrimental impacts by the 921 earthquake. With great help from various levels of governments and non-govermental organizations(NGO), the people in the Se-Shui dev...

I-hsiao Chung, 鍾宜孝

碩士 / 國立成功大學 / 醫學檢驗生物技術學系 / 95 / 10-formyltetrahydrofolate dehydrogenase (FDH) converts 10-formyl- tetrahydrofolate (10-formyl-THF) to tetrahydrofolate (THF) and participates in the biosynthesis nucleotide precursor. It has been shown that FDH was down regulated during cell proliferation while over expression of FDH induced cell cycle arrest and apoptosis in cancer cells. Therefore the expression regulation of FDH is believed to tightly correlate to tumorgenesis and embryonic development. The aim...

Hung-chang Lin, 林宏彰

碩士 / 國立成功大學 / 醫學檢驗生物技術學系 / 95 / 10-Formyltetrahydrofolate dehydrogenase(FDH), a folate-requiring enzyme in one-carbon metabolism, catalyzes the NADP+-dependent oxidation of 10-formyltetrahydrofolate (10-CHO- H4PteGlu) to form CO2 and tetrahydrofolate. This is the the only pathway responsible for formate oxidation in methanol intoxication in primates. Mammalian FDH is a homotetramer composed of 99 kDa subunits. Each monomer consists of a small N-terminal domain and a big C-terminal domain. The...

Shu-Yung Chiu, 邱淑雍

碩士 / 國立嘉義大學 / 獸醫學系研究所 / 96

Li-Yun Juan, 阮莉芸

碩士 / 國立彰化師範大學 / 生物學系 / 96 / Protein phosphatase 4 (PP4) complex is a member of phosphoprotein phosphatase family. Its mainly function is to remove phosphate groups from phosphorylate serine/threonine in proteins. Through this way, PP4 is able to regulate a variety of cellular functions. PP4 complex is consisted of two subunits, one is termed catalytic subunit (PP4C) which can transfer phosphate group from substrates, the other is termed regulatory subunits (PP4R1, PP4R2, and PP4R3) which regulat...

Lin-Yoan Chou, 周林源

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 94 /

Chain-zin Liou, 劉千子

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / Abstract Common carp always has high Zn in its tissue. Crucian carp (Carassius auratus) and common carp both belong to the Cyprinea of Cyprinidae. In order to know whether high Zn also exists in crucian carp, the distribution and characteristics of the Zn in crucian carp were studied. It was found that in the digestive tract tissue and kidney of crucian carp, the Zn concentrations were high, 807±503 and 126±54 μg/g fresh tissue, respectively. However, in hepatop...

Chih-Chieh Chang, 張智傑

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / Abstract Common carp is highly anoxia tolerant. It was known that high tolerance to anoxia in common carp is related to its high concentration of Zn in the digestive tract tissue. Besides, it is also known that the high Zn in common carp located in fibroblasts. This research studied the relation of zinc, hydroxyproline, proline and Zn-binding protein in the digestive tract tissue of common carp during anoxia. The experiment result showed that when dissolve oxygen in ...

Chun-Kai Fang, 方俊凱

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / The aim of this study was to establish the isolation conditions to characterize the physical and properties of collagen from the skin waste material of hybrid tilapia (black tilapia (Oreochromis mossambicus) cross red tilapia (Oreochromis nilotica)) by solvent extraction. The physical-chemical properties include amino acid analysis, circular dichroism (CD) , sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), denaturation temperture (Td) to charact...

Sheng-Ching Wang, 王聖欽

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / Abstract This study investigated the antioxidative activity in the hydrolysates of egg albumin protein (EAP), whey protein concentrate (WPC), soy protein isolate (SPI) and Tilapia muscle protein (TMP) by using the crude extract of tilapia viscera as the enzyme source. Protein substrates were hydrolyzed at 50℃ for 12 hours at different pH values (4.0, 7.0 and 9.0).The lyophilized hydrolysates were then measure for the scavenging effect on α,α-diphenyl-β-picrylhy...

Hui-Jung Pu, 濮譓蓉

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / Two experiments were conducted to evaluate the sparing effect of two dietary vitamin C derivatives, L-ascorbyl-2-monophosphate-Na (C2MP-Na) and L-ascorbyl-2-monophosphate-Mg (C2MP-Mg), on iron (Fe) requirements of juvenile grouper (Epinephelus malabaricus). Experiment I. Basal diet with 127 mg Fe/kg and 76 mg Fe/kg which were thought to be adequate and inadequate, respectively of Fe requirements for grouper, each supplemented with either adequate, 3X adequate and 5X ...

Yao-Sheng Chen, 陳耀甥

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family that includes NGF, NT3, NT4/5 and NT6. BDNF play a critical role in the regulation of neuron survival and growth. BDNF gene has a complex structure with multiple regulatory elements and multiple promoters that are differentially expressed in central or peripheral nerve tissues. In this study, we have designed primers for the amplification of BDNF exon 2 and used this DNA fragment as a p...

Hsin-Chi Liao, 廖信吉

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / Previously, we have isolated KLF8 cDNA from zebrafish and it encodes 348 amino acids that sharing high (63%) amino acid sequence similarity with human KLF8. Zebrafish KLF8 protein has three tandem Cys2His2 zinc-finger motifs at the carboxyl terminus. In addition, a repressor motif (PVALS/T) is located at the N-terminus of KLF8 protein. To explore the function of KLF8 on zebrafish development, we used KLF8 specific antisense morpholino oligomer to knock-down KLF8 ex...

Jia-Jiun Tsai, 蔡佳均

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / The research made a study of the five days feeding course in tilapia with 25, 50, 75, 200 mgkg/day oxytetracycline additive feed and withdrawal time for 20 days. At day 0,1,2,3,4,5,6,7,9,13,17,21,25, examined the drug residues in fish muscle and serum. In addition, at day 0,1,2,3,4,5,6,7,9,13,17,21,25, examined non-specific immune immunological parameters and physiology parameters such as superoxide anion (O2-) production ratio,total WBC count, superoxide dismutase ...

Lee-I-Yang, 李毅揚

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / This study investigated the efficacy of Aeromonas hydrophila vaccines prepared from different culture media, in tilapia. The main content included the detection of protease activity and haemolytic units variation of ECP of Aeromonas hydrophila grown on different media, survival rates of tilapia preimmunized with various vaccine, rocket immuno-electrophoresis and agglutinating activity of tilapia antisera. The medium, TSA + O.salts + 2 % glucose + 100 μM 2, 2-dipyr...

Ann-Chang Cheng, 鄭安倉

博士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / Abstract The effects of energy generated from fishmeal protein replace by other energy sources in orange-spotted grouper (Epinephelus coicoides) based on isoenergy were investigated. Two major experiments were conducted with control diets containing either higher or lower protein content than the requirement of grouper, respectively. In the experiment of control diet containing lower dietary protein (CP 45% from fishmeal protein) than the requirement, the results in...

Yi-Hua Chen, 陳怡樺

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 93 / Retinoic acid (RA) is metabolite of vitamin A, and is indispensable for cell differentiation, and cell proliferation during vertebrate embryonic development. Endogenous RA is a morphogen, while exogenous RA is a teratogen. During early heart development, RA signaling pathway can regulate cardiomyocyte population size and subsequently it regulates heart looping process. RA is indispensable signal molecular for the normal development of cardiovascular system. Therefo...

Pei-Chun Chen, 陳佩君

碩士 / 臺中健康暨管理學院 / 生物科技學系碩士班 / 93 / Abstract The effects of storage temperatures on the composition and antioxidative activities of one kind of Taiwanese yam tubers,Tainung No.1(TNG1) (Dioscorea alata),were investigated at room temperature (20±8℃),17±2 and 10±1.5℃. Measurements of antioxidative activities included reducing power and α,α-Diphenyl-β-pricryl-hydrazyl(DPPH) radical-scavenging activity. The crude lipid and fiber contents decreased with storage time at all three temperatures, but the re...

Hsiu-Yueh Tsai, 蔡秀月

碩士 / 國立高雄師範大學 / 生物科學研究所 / 93 / Tilapia (Oreochromis mossambicus) is among one of the most important species in the aquaculture industry world wide. Streptococcus agalactiae infections have impacted significantly the local tilapia industry in Taiwan. The infection has resulted in high mortality and heavy loss every year, especially during the months with elevated water temperature. Affected fish show hemorrhage on the body surface, darkening of the body, corneal opacity, reduced appetite and sple...

Ying-Chen Chen, 陳瑩珍

碩士 / 國立中興大學 / 農藝學系 / 93 / Thirty-three lines of Hottuynia cordata Thunb. in Taiwan were cultured at the same locality and then used to investigate the intra-specific variation of anatomic characters, mainly included oil cells, starch grains and crystals, with paraffin sectioning. Data was analyzed by cluster and PCA procedure of MVSP software. From the cross sections of tissues, lots of oil cells are distributed in the large parenchyma in contact with the epidermis of leaf, stem and rhizome. Oil...

Linweien, 林偉恩

碩士 / 國立高雄師範大學 / 科學教育研究所 / 95 / Variation of the mitochindrial DNA cytochrome b and population genetics of the grey mullet (Mugil cephalus) in the western Pacific fringe The cirumtropically distributed grey mullet (Mugil cephalus) which occurs in waters surrounding Taiwan on the western Pacific fringe is divided into at least two stocks (migratory and residential), but questions regarding the level of genetic divergence and gene flow between them remain to be answered. To cast more light on this,...

Yao-Lin Chang, 張曜麟

碩士 / 國立高雄師範大學 / 生物科學研究所 / 94 / Numerous studies have shown that high levels of mercury are harmful to both humans and wildlife. The concentration of mercury has been determined in four species of marlins for a survey of the bioaccumulatable contaminant. Samples were prepared using standard digestion procedures and analyzed by flameless atomic absorption. Seventy samples were collected from Southern China Sea. Among the four species analyzed, three species illustrated concentration level of ...

Ta-Kun Shen, 沈大焜

碩士 / 國立臺灣海洋大學 / 應用經濟研究所 / 92 / After becoming a member of WTO, impacts on traditional pattern of production and marketing of grouper farming shall be inevitably brought by global competition and transition in domestic business environment.Therefore, integration of upstream, mid stream, and down stream in production and marketing channels, and planning for a highly efficient supply chain that covers consumer demands, production, and distribution, are the keys to the solution of current problems i...

Huei-Wen Shr, 施惠文

碩士 / 國立臺灣海洋大學 / 應用經濟研究所 / 92 / 英文提要: Taiwan is one of the major farmed-grouper producing countries all over the world and its also high economical aquaculture product in Taiwan. In the recent years its supply and demand increase greatly which change the aggregate structure of seafood market in Taiwan. This study is based on 191 samples on grouper farming from Annual Economics Survey of Offshore Fisheries and Aquaculture in Taiwan, from 1999 to 2002. We provide a comparative analysis of the pr...

Hu-chen chen, 陳玉真

碩士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Abstract Tilapia were fermented with 4% Sucrose, 1% Glucose, 2% NaCl, 50% Distilled water, and lactic acid bacteria (LAB) strains of Pediococcus (Ped.) pentosaceus MFL, Ped. pentosaceus MFS, Lactobacillus (Lb.) plantarum BCRC12250, or their combination, and non-starter. LAB were as starters in tilapia meat paste. After 12 hr fermentation, the pH value rapidly declined to under 4.6 and cut down fermented time to 12 hr. The LAB growth rapidly, became predominant du...

Lung-Ying Hsu, 許瓏瑩

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 92 / ABSTRACT A high expression of heat shock protein 70 (HSP70) was found to arise in zebrafish (Danio rerio) at 72 to 84 hr after fertilization [36, 37]. The spontaneously expressed HSP70 was later shown to comprise predominantly heat shock cognate 70 (HSC70), HSP70 synthesized under nonstressful condition, and very low level of stress-induced HSP 70 [53]. Owing to the constitutive expression of hsp70/hsc70 mRNA in developing zebrafish, the spontaneous production o...

Li-Feng Chir, 闕立楓

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 92 /

Jian-Shiung Wu, 吳建勳

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 92 / Recently, a novel bHLH-PAS transcriptional factor family was revealed to play an important role in vertebrate development. SIM, HIF and AHR can form heterodimers with a central bHLH/PAS partner protein, ARNT, to modulate variety of biological functions, including neurogenesis, angiogenesis and vasculogenesis, and biological circadian rhythm. Previous studies in our laboratory have shown that repression of arnt2a/b/c or hif1a+hif3a by morpholino oligonucleotides (M...

Kai-Chung Ho, 何繼忠

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / 英文摘要 The effects of different storage temperature and time cause on the serum physiological and biochemical parameters of grouper (Epinephelus coioides) were investigated. Ten Adult Epinephelus coioides were used. Epinephelus coioides were bleeded and put every two Epinephelus coioides blood into one test tube. Experiment is divided into A, B, C, D and E five groups. Blood samples were collected and stored in 4 ℃, -20 ℃ and –80℃ respectively. These blood samples we...

Lyu Yu Fen, 呂玉芬

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 92 /

Ya-Ling Tung, 童雅玲

碩士 / 國立臺灣海洋大學 / 食品科學系 / 92 / To improve the quality and functionality and consequently increase the utilization of fish muscle, the mackerel muscle protein was hydrolyzed by the proteases from Aspergillus oryzae Malt Extract broth, and then fermented by lactic acid bacteria. The highest proteases were obtained from Asp. oryzae after 72 hr incubation at 25oC.The acidic protease activity was much higher than neutral and alkaline proteases. SDS-PAGE indicated the obvious degradation of mackerel mus...

Ming-Shyong, Wang, 王明雄

博士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Concentrations of Zn in most tissues of several studied mammalian species are in the order of 10-100 mg/(g wet weight), with little variation among species. The average Zn content of the edible portion of finfish is lower, at 6.5 mg/(g wet weight), with a range of 3-24 mg/(g wet weight). However, the common carp Cyprinus carpio has an extraordinarily high Zn concentration of 300-500 mg/(g fresh tissue) in its digestive tract tissue. Subcellular fractionation of th...

Wei-Ming Wu, 吳偉銘

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 92 / Abstract Krupple-like factors (KLFs) are DNA-binding motif containing transcription factors that found in many organisms. Studies indicate that they play key roles in cell growth, differentiation, embryogenesis, and tumorigenesis. In mouse, KLF4 and KLF5 are expressed in epithelia of gastrointestinal tract, and are required for proliferation and differentiation of gut epithelial cells. Thus, these KLFs are important for digestive tract development. Recently, zebra...

Chang-Hsue Sun, 孫昌旭

碩士 / 元智大學 / 資訊工程學系 / 94 / In our research , we propose to well use the Global Positioning System “ (GPS) [17] and combine with “ Fisheye State Routing “ (FSR) [9] in the Mobile Ad Hoc Network. In the “Table-Driven” Algorithm , FSR[9] can effectively reduce the routing message overhead in the large network by making periodic information update . The size of update messages are smaller as it uses the Fish-Eye Technique [8]. The information exchange will more frequently between the near-by nodes a...

Yung-Chang Lin, 林永蒼

碩士 / 淡江大學 / 生命科學研究所碩士班 / 94 / The vertebrate skeleton consists of cartilage and bone. Cartilage originates from chondrocyte and bone from osteoblast and osteoclast. CBFA (core binding factor alpha subunit) belongs to the runt homology domain family, that promotes the osteoblast differentiation and bone formation in early development and also inhibits osteoblast differentiation during late development. This dual functions of the CBFA maintains the balance of the bone formation and resorption. cbf...

Sih-Wei Huang, 黃思維

碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 94 / The objectives of this study were to investigate the difference and interaction of mercury (Hg) and selenium (Se) among fish species and tissues. In addition, by comparing with the detected concentrations in other regions, it would be possible to evaluate the pollution status. In the meanwhile, this study analyzed the edibility of the fish, the interaction between Hg and Se as well as their impacts on fish growth. Ten species of fish, including tenpounder (E...

Ying-ling Hsu, 許櫻齡

碩士 / 國立中山大學 / 化學系研究所 / 94 / none

Yu-Jei Lei, 雷玉潔

碩士 / 中國醫藥大學 / 醫學研究所碩士班 / 94 / The aim of this study was to investigate the neuroprotective effect of selective estrogen receptor (ER) agonists on the nigro-striatal system. Parkinsonism was induced in ovariectomized rats by treatment of rotenone via subcutaneous osmotic minipump (3 mg/kg/day) for 2 weeks. These rats simultaneously received daily injection of estradiol (10 ug/kg) or selective estrogen receptor (ER) agonists （1 mg/kg）. Locomotor activities were observed before and 2 weeks after ro...

Chi-Wei Lo, 羅智緯

碩士 / 國立東華大學 / 生物技術研究所 / 93 / The information related to environmental adaptation-associated genes in fish brain is rare. The aim of this study was to investigate the expressions of salinity adaptation- related genes in euryhaline tilapia (Oreochromis mossambicus) brain upon acute salinity challenge. Isolated whole brain mRNA of freshwater (FW) tilapia and of 25 ppt seawater (SW) transferred at various times (1, 2, 4 or 8 h) tilapia were applied to do PCR-select subtractive hybridization. After c...

Lee Yu-Li, 李毓禮

碩士 / 國立臺灣師範大學 / 設計研究所在職進修碩士班 / 94 / ABSTRACT Colors and veins are the major characteristics of determining the appearance of objects. Veins or quality, shapes and colors are the three major elements of making appearance. Except the shape of object, colors and veins are the most direct elements of operating visual mind. “Light” makes people see everything. There is the light, there is the color. It makes people see various colors, veins and shapes. People enjoy different kinds of beautiful scener...

JENG YIN WEI, 鄭穎蔚

碩士 / 國立臺北教育大學 / 自然科學教育學系碩士班 / 95 / This experiment, from February 2006 to January 2007, was designed to learn the habit and breeding behavior of Macropodus opercularis (Linnaeus) in an artificial environment. Paradise fish’s behaviors were categorized into nine patterns in this experiment. Among these patterns, swimming took 47.6%, feeding took 20.4%, motionless took 17.8%, and the rest behavior patterns including surfing to breath, building nest, taking care of larvae, waving the fins, and wavi...

Chien-Hao, 黃建豪

碩士 / 中山醫學大學 / 生化暨生物科技研究所 / 94 / To date, it is well accepted that Regulator of G-protein signalling（RGS） proteins terminate signalling by accelerating the intrinsic G alpha-GTPase activity and recycling the G-protein complex back to its inactive GDP-bound heterotrimeric configuration. The RGS protein can be divided into 15 subfamilies（RGS1~RGS15） in accordance with the structure and the biological function. The RGS domain, a conserved 120-aa region exists in all RGS proteins, functions as a nega...

Chao-Kun, 劉兆坤

碩士 / 中山醫學大學 / 營養學研究所 / 94 / In this study, the search tool BLAST (Basic Local Alignment Search Tool), based on NCBI (National Center for Biotechnology Information) was used against nucleotide and protein databases. The analysis found a Dimeric Dihydrodiol Dihydrogenase (dimeric DD) of zebrafish (NCBI accession number BC081393/ AAH81393) and human (NCBI accession number NM_014475/NP_055290). The dimeric DD cDNA of zebrafish and human were successfully amplified with a continuous open reading fram...

Yi-Hao-Tsai, 蔡怡豪

碩士 / 國立臺北科技大學 / 土木與防災技術研究所 / 92 / The Jiji earthquake, measured and reported by the Central Weather Bureau, had the maximum horizontal ground acceleration of 989.2gal, and vertical ground acceleration of 519.4gal. The vertical acceleration value is approximately one half of the horizontal value, V = H/2. A big vertical motion may throw objects into the midair and then let them fall down to the earth, resulting in a suddenly collapse of objects. The research focuses on the influences of the verti...

Chun-Hung Lu, 呂俊泓

碩士 / 國立臺灣大學 / 漁業科學研究所 / 92 / Type I (acidic) and type II (basic) keratins, two major classes of intermediate filament proteins, is encoded by at least 30 genes and are mainly expressed in epithelial cells in mammals. The regulation of keratin expression and transcription factors have been studied in mammals. However, the role of regulation is still unclear although there are many studies on the expression pattern of keratin in zebrafish (Danio rerio). In this report, we characterized an upstream...

Yu-Ting Jeng, 鄭宇婷

碩士 / 國立臺灣大學 / 漁業科學研究所 / 92 / The tetracycline (Tet) –controlled system is well developed in many transgenic organisms. Yet, this system has not been reported in zebrafish. DNA construct, rtTA that is driven by cmlc2 promoter acts as an activator of TetO, was microinjected into the one-celled stage zebrafish embryos and there of germ-lines were generated. We examined the germ-line transmitted F2 embryos; we found that the increase of GFP expression was tightly dependent on the higher Dox dosage (...

Shu-Chien Chung, 鐘淑鈐

碩士 / 國立臺灣大學 / 微生物與生化學研究所 / 92 / Insulin-like growth factors (IGFs) were discovered in human in 1963 and named because of the similarity to insulin in chemical structures and functions. IGFs commonly exist in vertebrate and play an important role in the metabolism and stimulate mitosis in cells. Recently, many efforts have been made to express IGFs gene in E. coli. Production of high yields of recombinant proteins in heterologous hosts such as E. coli and their recovery from the resultant biomass...

Chia-Lin Wang, 王嘉琳

碩士 / 國立臺灣大學 / 漁業科學研究所 / 92 / The tetracycline-controlled myocardium expression system was used to study zebrafish cardiac troponin C (cTnC), a calcium binding protein specifically expressed in heart. GFP reporter and antisense strand of cTnC were constructed on both side of the bi-directional promoter, which was response to the doxycycline (Dox)-induced transactivator driven by a heart –specific promoter of cardiac myosin light chain 2. GFP reporter gene was specifically expressed in the heart o...

Wei-Chuan Chiang, 江偉全

博士 / 國立臺灣大學 / 海洋研究所 / 92 / Age-growth and reproductive biology of sailfish were studied and the results were applied to yield per recruit (Y/R) and spawning stock biomass per recruit (SSB/R) analysis to assess the current stock of sailfish in the waters off eastern Taiwan. Length and weight data were collected monthly from a total of 6,279 sailfish (1,927 females and 4,352 males) landed in Shinkang Fish Market during July 1998 to December 2002. The fish sizes ranged from 78 to 239 cm LJFL (lower...

Jenn-Jye Shiao, 蕭振杰

碩士 / 國立中興大學 / 農藝學系 / 93 / Summary The twenty-five collections of Houttuynia cordata Thunb. collected from Taiwan were used as materials to investigated the variation of plant parts , crop seasons and growth stages for shoot dry matter, the contents of flavonoid glycosides and total polysaccharides. In addition, the estimators, such as heritability, correlation analysis and the principal component analysis were estimated. The results were summarized as follows： 1. After investigation, the dr...