Liang, Shu-Yang, 梁書暘

碩士 / 弘光科技大學 / 生物科技研究所 / 105 / Thousands of years ago, Terminalia chebula was used to prevent neural disease. In India, because of its high medicinal value, it is used to treat various diseases. We use scientific methods to understand the pathway of T. chebula preventing Pa r k i n s on ’ s di s e a s e （PD）. Rat adrenal medulla pheochromocytoma（PC12 cell）is used to simulate neurons in nigrostriatal system. To simulate PD injure pathway, rotenone and paraquat are used as damage agents. PD is a de...

XU,Fang-Yuan, 許芳源

碩士 / 銘傳大學 / 生物科技學系碩士班 / 104 / The feasibility of aquaponics farming in business requires cost considerations. In this study, we tried to integrate the cultivation of wasabi and eel as the basis for aquaponics. The study was conducted from December 2015 to June 2016. The result shows that only four eels died in the two systems, it also tells us that eels are able to stand the variability of environmental factors. On food conversion rate, both systems were above 1.43, this result meets the expecta...

An, Ping-Zao, 安平兆

碩士 / 國立屏東科技大學 / 動物疫苗科技研究所 / 103 / Tilapia is one of the most important species in the aquaculture industry in Taiwan. However, the Streptococcus iniae often causes streptococcosis in fish farming and results in serious economic losses. Virulence factors, such as glyceraldehyde 3-phosphate dehydrogenase (GAPDH), play critical roles in the host-invasion process to assist bacteria to escape from host immune system. Thus, GAPDH protein could be a key candidate in the vaccine development against S. i...

Chung, Chung-Min, 江仲民

碩士 / 國立屏東科技大學 / 生物科技系所 / 103 / Over the decades, nanotechnology has become a rapidly developing industry, therefore nanoparticles concentration increased in the air, causing potential risk to organisms. TiO2-NPs have been widespread used in consumer products, such as cosmetics, sunscreens and building paints. According to statistics, manufacturing products containing TiO2-NPs stand at 5000 t/a for the current production. The phototoxicity of titanium dioxide nanoparticles (TiO2-NPs) can produce ...

Chen, Nien-Chi, 陳念岐

碩士 / 國立屏東科技大學 / 水產養殖系所 / 103 / Abstract: The study was to evaluate the effects of water temperature on growth, feed intake and body composition of juvenile barramundi (Lates calcarifer). Triplicate groups of fish (initial wt:10 g) were reared in recirculating system with five temperature (23 °C, 26 °C, 29 °C, 32 °C and 35 °C) for 30 days. Survival (100%) were the same (p > 0.05) among all treatments. Feed intake and specific growth rate (SGR) were the highest (p < 0.05) in 29 °C and 32 °C rear...

Tsung-YuHan, 韓宗佑

碩士 / 國立成功大學 / 生物科技研究所 / 104 / In vertebrates, body axis formed during early embryogenesis. According to the earlier studies, Wnt signaling pathway controls dorsal-ventral body axis in blastrula and controls anterior-posterior body axis in gastrula. In human, Myc gene is at down stream of Wnt signaling pathway and the product of Myc gene is a transcription factor that involved in regulating many cell functions including cell proliferation or cell cycle progression. In previous study, Dawid’s lab ...

Zhu Yi Ting, 朱怡婷

碩士 / 國立高雄海洋科技大學 / 海洋工程科技研究所 / 97 /

Shin-Hong Yeh, 葉信宏

碩士 / 國立屏東科技大學 / 獸醫學系所 / 101 / Bacillus spp. infection was an Emerging disease in soft-shell turtle. Total of 34 cases（56 turtles）collected, all turtles had bacillus spp. infection, especially in those aged over one year（53/56, 95%）. Bacillus-infected turtles showed the clinical signs of swollen and whitish neck and eyes. The gross lesions included hepatic congestion and enlarged spleen. Histopathological features were characterized by the formation of multiple focal necrosis and bacterial clumps...

李薏姍

碩士 / 國立屏東科技大學 / 食品科學系所 / 101 / The thermal gelation of cross-linked milkfish muscle proteins with 1-ethyl-3-（3-dimethylamino-propyl）carbodiimide（EDC）was studied by means of jelly-strength , ATP sensitivity , the activities of ATPases , SDS electrophoresis and chymotryptic digestibility. The treatment with EDC（0.1mg EDC/mg protein）gave higher jelly-strength values to the actomyosin for all the tested temperatures. Gel-disintegration for actomyosin and myosin was completely depressed by EDC. By tr...

江潁彥

碩士 / 國立屏東科技大學 / 生物科技系所 / 101 / Genetically modified (GM) fish have been investigated widely in diverse fish species for improving quality. However, commercially available GM fish are restricted except transgenic ornamental fish because of global concerns the ecological impacts including gene flow and extinction of wild natural fish stock. In this regard, infertility control is a core technology for overcoming this issue. In this study, we use zebrafish to establish a platform technology of infer...

Jung-Kuang Wang, 汪榮光

碩士 / 國立屏東科技大學 / 生物科技系所 / 101 / Anti-Mullerian hormone (AMH), also known as Mullerian inhibiting substance (MIS), is a glycoprotein belonging to the transforming growth factor-β superfamily (TGF-β). Reports have shown that teleost AMH is expressed predominantly in the reproductive tissues and acts an important role in gonad development and sex differentiation. In recently years, although the medium-sized fluorescent angelfish (P. scalare) has been established, it is still refused to commercialize...

Yun-Pei Yang, 楊昀珮

碩士 / 國立屏東科技大學 / 動物疫苗科技研究所 / 101 / Orange-spotted grouper (Epinephelus coioides) is an economically important farmed fish in southeast Asia, including China and Taiwan. Infecting with diseases is causing huge loss in aquaculture industry. In Taiwan, the disease rate of farming fish has increased tremendously due to the way of high-density farming fishery, which resulted in high death rate of fishes and shells. The economic consequence has been devastating. Vibriosis is one of the most serious dis...

Guan-Yu Lai, 賴冠宇

碩士 / 國立屏東科技大學 / 食品科學系所 / 101 / Soft-shelled turtle (Pelodiscus sinensis) is commercially cultured in China, Japan and Southern Taiwan area, and for its good nutriment and medical values. Because the embryo extract from soft-shelled turtle eggs has lots of nutriment for embryo growth, it is beneficial to human health. After soft-shelled turtle eggs form embryo, the eggs were dried in 100℃ oven to make embryo extract (PSE). The PSE contains 29.6% crude protein, in which the main amino acids are gl...

Chen,Ciao Ting, 陳巧婷

碩士 / 國立清華大學 / 生物資訊與結構生物研究所 / 103 / Epilepsy is a brain disorder that develops spontaneously. Recurring seizures are caused by abnormal neuronal activity in the central nervous system (CNS). The complex etiology of epilepsy is shown to contain multiple causes, including chemical induction, brain injury and genetic factors, which further complicate the analysis. Thus, it is still not possible to predict the occurrence of epileptic seizure due to lack of understanding of its disease mechanism. Prev...

Li-Hsin Shu, 舒立欣

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 101 / Metallothionein (MT) has been shown on the heavy metal detoxification. In addition, it will be induced by variety of chemical substances in organisms. In the past studies, it well knows that the physiological roles of MT-2 of zebrafish exposed to cadmium environment, and it may be associated with the cadmium-detoxification, but the physiological roles of similar to metallothionein B (sMT-B) was still unclear. Therefore, the present study will explore the physiolo...

Chuo-nan Huang, 黃卓男

碩士 / 國立臺灣大學 / 獸醫學研究所 / 89 / The preproaerolysin of Aeromonas hydrophila isolated from ayu was cloned and expressed. The complete preproaerolysin gene was 1461 base pairs, which contained 487 amino acids, and the molecular weight was about 59kDa. The recombinant preproaerolysin retained its toxicity after activated with trypsin, and the property was deactivated by treating at 46℃ for 5 min. Hemolytic ability on blood agar and cytolytic ability on Vero cell was noted in vitro. It caused the death ...

黃順國

碩士 / 國立臺灣大學 / 漁業科學研究所 / 89 / Red striped snapper (Lutjanus erythropterus) grow slowly, it about need two years to reach the marketing size 600-1000g. The aim of this study is to evaluate that the effect of Insulin-like growth factor (IGF) of tilapia procreate from recombinant E.coli add to feed of red striped snapper. Experiment one:Fish meal is the main protein source in diets, the three diets separately control, to add IGF contained in E.coli 0.04g, IGF contained in E.coli 0.4g ...

Yu-Hua Huang, 黃玉華

碩士 / 國立臺灣大學 / 漁業科學研究所 / 89 / The purpose of the present study is to examine the expression and activation of Na+- K+-ATPase α isoforms in tilapia (Oreochromis mossambicus) during seawater (SW) adaptation. Plasma osmolarity and enzyme activity and protein expression of Na+- K+-ATPase in gills and brain were measured in tilapia transferred from freshwater (FW) to 25 ‰ SW. The results showed that plasma osmolarity increased 3 hr after transfer, then started to decrease at 48 hr, and recovered to th...

Yi-Hsuan Chen, 陳以萱

碩士 / 國立臺灣大學 / 漁業科學研究所 / 89 / Crystallins have been recognized as the main structural protein of eye lens in vertebrates. The transparency and refractive properties of the lens are presumably linked to the high concentration of lens crystallin. However, the structure and function of beta-crystallin is the least understood to date among the three major crystallin families. In this study, betaB1-crystallin is molecularly cloned and sequenced from zebrafish. The expression and distribution of betaB1...

Chia-Ling Hsu, 徐佳鈴

碩士 / 國立中山大學 / 海洋生物研究所 / 91 / Dicyemid mesozoans are endoparasites inhabited in benthic cephalopod molluscs. Previous studies of dicyemids were mostly concentrated on their taxonomy. The life cycle remains a mystery since Cavolini observed this animal in 1787. The purpose of this study is to investigate further on the dicyemid life cycle building on the limited knowledge from previous studies, and investigate the infection of dicyemid in octopuses in tropical waters, using the area in the southwe...

Chia-Chin Hsu, 許嘉錦

碩士 / 國立中山大學 / 海洋生物研究所 / 91 / Traditional morphometric method measures the linear distance between two points on the body surface. Statistical techniques, mainly covariance analysis and principle component analysis, are respectively used in single- and multi- variable statistical inferences. Their purpose is to overcome the bias due to allometric growth. Geomorphometric methods (mainly superimposition method) studied the size and shape of organisms was developed at recent decade. These methods no...

Hsu-Hui Cheng, 鄭旭惠

碩士 / 南華大學 / 環境管理研究所 / 91 / The coastal regions of southwestern Taiwan are subjected to arsenic (As) pollution from groundwater. To use the groundwater for aquaculture may cause an overexposure of arsenic.The bioaccumulation, as well as acute and chronic toxicities of As, in aquacultural milkfish (Chanos chanos) from blackfoot disease (BFD) area were studied. Water and milkfish samples were collected from the milkfish farms in the BFD areas of Putai, Yichu, Pemen and Hsuechchia, respectively. The...

Ni-yen Hsieh, 謝妮諺

碩士 / 國立高雄第一科技大學 / 行銷與流通管理研究所 / 102 / (紙本延後至108814止公開)

Ting-yun Wu, 吳婷芸

碩士 / 國立中山大學 / 生物科學系研究所 / 103 / Vascular development is important for vertebrates and regulated by regulators via complicated signaling pathways. Enzymatic protein to control activation of signalings is critical but is not well understood. Zebrafish is a powerful vertebrate model organism to study genetic control vascular patterning. In our previous study, transcription factor Isl2/CoupTF1b regulated vein and ISV formation and genome-wide transcriptional analysis suggested that cpn1 is regulated ...

Tzu-wei Liu, 劉子維

碩士 / 國立中山大學 / 海洋科學系研究所 / 103 / Water depth and the amount of suspended particles shape the light spectrum of an aquatic environment. In clean open water, the full spectrum is attenuated to blue right as the depth increases. However, shortwave blue light is absorbed by suspended particles in turbid water, leaving a red light dominant photic environment. Gobiidae are distributed widely in various habitats with varying photic environments, making them good candidates to study the relationship of ha...

Lu-Hsueh Huang, 黃律學

碩士 / 國立臺灣大學 / 環境衛生研究所 / 103 / Food restriction, one of the environmental stresses, could produce serious effects to organisms and may cause to population decline and extinction. In different life stages, wild fish often encounter to food restriction because they usually expose to extreme environment or special status, such as climatic change, low temperature, environmental pollution, habitat destruction or spawning migration. Green sturgeon was listed as ‘‘threatened’’ in 2006 under the U.S....

吳佳珍

碩士 / 國立政治大學 / 新聞研究所 / 102 / In March 2013, an international dispute directly related to Taiwan occurred—Japan unilaterally "nationalized" the Diaoyutai Islands. This dispute relates to the sovereignty controversy which invoked radical patriotic movements among the concerned countries. This study examined the Diaoyutai-related news coverage from United Daily News, China Times, Liberty Times and Apple Daily to compare the framing differences among the newspapers. Furthermore, this study tried to u...

Lee,Tsung-Han, 李宗翰

博士 / 國立臺灣大學 / 動物學系 / 84 / MR細胞(mitochondria-rich cells)為硬骨魚類鰓表皮上進行離子與滲透 壓調節的主要部位。初步實驗手先以掃描式電子顯微鏡觀察發現MR細胞在 廣鹽性吳郭魚(Oreochromis mossambicus)鰓表皮上以三種不同的形態存 在，即突起型(wavy-convex type)﹑淺盤型( shallow-basin type)﹑深 洞型(deep-hole type)。穿透式電子顯微鏡觀察結果證實其細胞質中都具 有MR細胞的特徵﹕大量的粒線體以及密佈的管狀系統。隨後發現在離子濃 度不同的低張環境中，吳郭魚MR細胞的形態與數量均有不同之處，而且同 一尾魚為適應環境，其MR細胞的形態與數量產生可逆性的變化。進一步由 離子添加實驗的結果得知，環境中高濃度的鈣離子會誘發突起型的出現﹔ ...

Chen,Jun-Chin, 陳俊欽

碩士 / 國立臺灣大學 / 生化科學研究所 / 84 / This essay explored the gene expression of carp egg membrane proteins during oogenesis. According to the sizes, five groups of carp oocytes were classified:perinucleolus stage, oil droplet- yolk vesicle stage, primary yolk stage, secondary yolk stage and tertiary yolk stage. Total RNA was first extracted from carp oocytes in order to quantify the ZP2、ZP3 and fibroin- like protei...

Tsao,Che-Chia, 曹哲嘉

碩士 / 國立臺灣大學 / 生化科學研究所 / 84 / Hyosophorin是在魚類卵細胞中發現的醣蛋白，為表層囊泡的主要成分。 其中醣類佔醣蛋白總重量的百分之八十至九十，且其蛋白質骨架完全由重 複序列接連排列而成。卵細胞進行表層反應時hyosophorin被釋出到胞外 ，同時各個組成的重複片段被裂解切開。本實驗室在對鯉魚卵巢高量表現 的基因進行初步研究時，發現了具有連續重複序列的cDNA，其序列樣式與 其他魚種的hyosophorin相似。為了驗證此等cDNA是否載錄鯉魚的 hyosophorin，本論文從鯉魚卵巢純化此醣蛋白，結果證實此純化產物的 氨基酸組成及N端的部分氨基酸序列與cDNA所推導者相符合。此外，大腸 菌表現之鯉魚重組hyosophorin所誘導的抗體能被純化的鯉魚hyosophorin 中和，因而...

Jiang, Zong Fen, 蔣宗芬

碩士 / 國立海洋大學 / 水產食品科學研究所 / 84 /

Zeng, Yi Ting, 曾儀婷

碩士 / 國立海洋大學 / 水產食品科學研究所 / 84 /

Zhang, Jia Ming, 張嘉銘

碩士 / 國立海洋大學 / 水產食品科學研究所 / 84 /

Lin, Ya Hua, 林雅華

碩士 / 國立海洋大學 / 水產食品科學研究所 / 84 /

Zhang, Sheng Xiong, 張勝雄

碩士 / 國立海洋大學 / 水產食品科學研究所 / 84 /

Li, Min Xian, 李民賢

碩士 / 國立海洋大學 / 水產食品科學研究所 / 84 /

Jean Chuen Tan, 簡春潭

博士 / 國立海洋大學 / 漁業學系 / 84 / onomy;allozyme;mtDNA;nucleotide sequencing;Taiwan 本論文使用形態學方法、同功異構■澱粉膠電泳法及粒線體 DNA核 ■酸序列分析法探討台灣周邊海域鯛亞科 5種魚類之種間親緣關係，並 使用後兩種方法探討黑鯛屬 4種魚類之種內族群遺傳。 鯛亞科5 種魚之種間親緣關係，在屬之水平上，三種方法之結果頗 為一致；但在黑鯛屬內 4魚種間之親緣關係上，則同功異構■澱粉膠電 泳法與粒線體 DNA核■酸序列分析法所獲得之結果一致，但形態學方法 所獲得之結果則與前二方法不一致。此不一致可能係不同形質之演化並 不一定是平行一致的，即所謂的鑲嵌演化所致。依據鯛亞科5 種魚之地 理分布及種間親緣關係，推論鯛亞科魚類之發源地在東印度群...

Shyu, Yuh-Ching, 徐玉青

碩士 / 國立海洋大學 / 水產養殖學系 / 84 / ABSTRACT Gene targeting is a technique for homologous recombination through the gene introduction, and can be applied on the recombination of gene knock-out and gene therapy. Previous studies on gene targeting were usually focused on murine embryonic stem cells to cultivate chimeric mice, which is helpful forfunction analysis of mouse genes. However, since up to now, no stud...

Chou Li-Wei, 周立偉

碩士 / 國立體育學院 / 運動科學研究所 / 87 / The muscle eccentric contraction immediately followed by concentric contraction is called " stretch-shortening cylce "（SSC）. In muscle performing SSC, it produces larger force output when comparing with non-SSC activities. This is due to the effects of elastic energy and myoelectrical potentiation. The purpose of this study was to analyze the SSC muscle performance in soleus muscles and compare the differences among squatting jump, countermovement jump and drop jump....

Shu-Chi Hsu, 許舒琦

碩士 / 國立中山大學 / 海洋資源學系 / 87 / Three groups of mullet (Mugil cephalus) including migratory, local and aquacultural mullets, were collected from Taiwan Strait, Da-pong Bay (S.W. Taiwan) and fish pond (S. Taiwan) respectively. Metal concentrations of Zn, Fe, Mn, Cu were analyzed in muscle, gonad and liver. In addition, the Ag, As, Cd, Co, Se and V concentrations in liver were also analyzed. The purpose of this study was to investigate the relationships between the metals concentration and ovarian mat...

Hsien-Chung Liao, 廖顯忠

碩士 / 國立中山大學 / 化學系 / 87 /

Hung-Ching Chiu, 邱洪磬

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 103 / Chromatophores distribution in the dermis determines the pattern and which is important functions in protection against UV irradiation, camouflage, kin recognition, shoaling and sexual selection in fish. The pigmentation is affected by the expression of coloration related genes, such as tyrosinase, tyrosinase-related protein 1/2 (TRP1/2), microphthalmia-associate transcripition factor (Mitf), sox10 and dopachrome tautomerase (Dct). These genes can induce differ...

Meng-Kai Lu, 呂孟鎧

碩士 / 中山醫學大學 / 醫學檢驗暨生物技術學系碩士班 / 101 / Copper is an essential element in living organisms as it is an important cofactor for many enzymes. At low concentration, copper is required for normal cell growth, whereas it is extremely toxic at a higher concentration. Atox1, a copper metallochaperone, plays an important role in the copper homeostasis. Atox1 transfers copper to the copper exporting P-type ATPases ATP7A and ATP7B. Recently, a novel function for Atox1 as a transcription factor (TF) for SOD...

Meng-Yen Ho, 何孟諺

碩士 / 中山醫學大學 / 醫學檢驗暨生物技術學系碩士班 / 101 / Glucose-6-phosphate dehydrogenase (G6PD) is the rate limiting enzyme in pentose-phosphate pathway. G6PD catalyzes glucose-6-phosphate to 6-phosphogluconolactone and concomitantly produced Nicotinamide adenine dinucleotide phosphate (NADPH). In cells, the NADPH serves as the reduced energy to eliminate the reactive oxygen species (ROS) and oxidative damage. In clinical, recent studies reveal the G6PD deficiency is associated with cancer, cardiovascular disease...

Shi, Yi Ru, 施怡如

碩士 / 國立臺灣大學 / 漁業科學研究所 / 83 / The grey mullet, Mugil cephalus L.,is one of commercially important teleosts suitable for culture. The ovarian maturation of female could be induced out-of-season by the enviromental manipulations of photoperoid and temperature. The steroid profiles of serum and the characteristics of serum steroid-binding proteins in grey mullet during oocyte maturation induced by hCG were monitored. S...

Hong, Shang Ren, 洪尚仁

碩士 / 國立臺灣大學 / 漁業科學研究所 / 83 / In this study the assessment of the early reduction of the Japanese anchovy (Engraulis japnoica) in I-Lan Bay was based on the nutritional status of the newly hatched larvae. The Japanese anchovy immigrate to I-Lan Bay to lay eggs in the spawning season. During the spawning season of 1994, from February to April, I took fortnight plankton samples, six times in total. Anchovy larvae were sorted from...

Zheng, Jian Guo, 鄭建國

碩士 / 國立臺灣大學 / 漁業科學研究所 / 83 / The cold front attacks Taiwan in winter causes lots of milkfish dead and badly influences this fishery field. This research is to find out the solution to this top urgent problem. The lipid deposited in fish affects their construction and physical characteristics may solve this cold problem to fish. The experiment was performent in two parts. Firstly the study was conducted to examine the different oils of n-3HUFA and secodly to examine cholesterol supplementation of...

Lin, Wen Xing, 林文興

碩士 / 國立臺灣大學 / 漁業科學研究所 / 83 / Deformed thornfish Terapon jarbua were abundantly found at the effluent of nuclear power plant NO.2 in summer 1993. Many possible reasons were pointed out, this study was set up with emphasis on two main factors: thermal discarge and chemical pollutant. After the environmental survey and the detection on deformed thornfish,zinc and tributyltin were found as the main pollutants at the effl...

SHEN, KANG-NING, 沈康寧

碩士 / 國立臺灣大學 / 漁業科學研究所 / 85 / Sicyopterus japonicus, a common species of amphidromous Gobiidae fish, widespread in the stream of eastern Taiwan. In this study we used theinformation achieved from otolith microstructure and microchemistry ( Sr:Ca ) of the fish collected in the Long-Long brook and its estuaryfrom September 1995 through April 1997 as well as environment factors to investigate the early l...

KUO, MING-WEI, 郭明偉

碩士 / 國立臺灣大學 / 漁業科學研究所 / 86 / In this study, the partial cDNA encoding for the sGnRH precursor,cGnRH-II precursor and sbGnRH precursor were isolated from tilapia brain using RT-PCR and 5''-RACE. The partial 145 bp cDNA encoding sGnRH precursor is composed of the biologically active sGnRH, the cleavage site (Gly-Lys-Arg), and a partial GnRH- associated peptide (GAP). The partial 366 bp cDNA encoding cGnRH-IIprecursor is ...