許峻源

碩士 / 國立陽明大學 / 醫學工程研究所 / 86 /

Jann-Jyh Yeh, 葉展志

碩士 / 臺北醫學大學 / 醫學科學研究所 / 97 / In this study, we evaluate a novel delivery system for oral vaccines, TFP-modified liposomes entrapped in acid-induced alginate (ALT) of biodegradable polymers, which is conceived from a combination of the polymer, the lipid–based delivery system and immunostimulant. We utilized liposomes (multilamellar, MLV) of 95 % PC to encapsulate 106.0 EID50/mL inactive H5N3 virus (LV group) and coated with Tremella fuciformis polysaccharide (TFP), purified from hot water extrac...

Ai-Ling Liang, 梁愛羚

碩士 / 國立中興大學 / 食品暨應用生物科技學系 / 96 / The purpose of this study was to investigate the stability of fucoxanthin in different solvent systems and the cellular uptake in two types of liver cells. A ethanol was used first to prepare a 10 mM fucoxanthin solution, which was then further diluted to 1.0 mM in ethanol (Stock A) or in 100% FBS (Stock B). A 10 μL Stock A was added to 10 mL DMEM medium containing 10% FBS (A1) or without containing FBS (A2). Likewise, A 10 μL Stock B was added to 10 mL DMEM medi...

Su-Ying Wong, 翁愫霙

碩士 / 國立中興大學 / 化學工程學系所 / 96 / In this study, the effect of nCDHA on chondrogenic differentiation of rat adipose-derived adult mesenchymal stem cells (ADAS) in alginate gel was evaluated. nCDHA/alginate composites containing different amounts of nCDHA were prepared and used to encapsulate rat ADAS. The chondrogenic differentiation of ADAS was enhanced after 7 days and the total collagen content increased after 14 days at 500 ppm of nCDHA. Cell proliferation was promoted in alginate with 100 ppm of...

Chih-Chieh Chen, 陳至潔

碩士 / 國立中興大學 / 食品暨應用生物科技學系 / 96 / Lactobacillus acidophilus possesses lots of important physiological functions beneficial to human health and numerous dried products which containing it are now popular for use as dietary adjuncts for humans and animals. However, low numbers of active L. acidophilus cells were found in the dried products. The freeze-drying method traditionally used for cells dehydration is slow and needs high cost. Therefore, competitiveness could be enhanced by means of the impr...

Huan-Chieh Chen, 陳煥傑

碩士 / 國立中興大學 / 食品暨應用生物科技學系 / 96 / Lactobacillus acidophilus is a popular strain of lactic acid bacteria and often added in consumer foods. L. acidophilus processes lots of important physiological functions beneficial to human health and numerous foods which containing it are now popular for use as dietary adjuncts for human. Freeze-drying is a popular method used in the culture preservation of microorganisms. The processes of freeze-drying might cost a lot of energy consumption and its freezing o...

Sheng-ji Lee, 李勝記

碩士 / 國立成功大學 / 工程科學系碩博士班 / 95 / This study describes the process of formation of monodisperse calcium alginate microcapsules by using microfluidic T-junction geometries. We focus on the investigation of the velocity fields and pressure fields. The flow inside the module is studied by means of computational fluid dynamics (CFD-RC®). Based on sheath force, Water-in-Oil (W/O) emulsions of an aqueous solution of a biopolymer are emulsified in an oil phase. In the external gelation method, these fine ...

Chin-Hua Hung, 洪金樺

碩士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Abstract There have been two food poison incidents due to ingesting the purple clam in Taiwan, and the causative organism was identified as the toxic dinoflagellate Alexandrium minutum. The toxin production of the dinoflagellate A. minutum was affected by nutritional and environmental factors. The toxic dinoflagellate A. catenella was belonged to Alexandrium spp. and had usually been reported in cold waters in several regions of the world. However, outbreaks of A. c...

Chia-Fen Lee, 李佳芬

碩士 / 國立臺灣大學 / 微生物與生化學研究所 / 92 / Photodynamic antimicrobial chemotherapy (PACT), derived from photodynamic therapy (PDT), utilized photosensitizer (PS) and visible or ultraviolet light in order to give a phototoxic response, normally via oxidative damage to microorganisms. Because neither of the two major factors (lights and PSs) of the PACT is inherently bactericidal, it could be a useful and simple way to treat and control antibiotic-resistant bacteria, such as methicillin-resistant Staphylococ...

Ye, Shou-Hung, 葉首宏

碩士 / 國立屏東科技大學 / 動物疫苗科技研究所 / 103 / Toxoplasma gondii, an obligate intracellular protozoan parasite, causes toxoplasmosis in a broad range of warm-blooded animals. Toxoplasmosis often causes abortions and fetal damage in livestock and pregnant women. The animals are infected by eating infected meat, by ingestion of feces of an infected cat, or by transmission from mother to fetus. Currently, only one T. gondii commercial vaccine is available for livestock, and only partial protection in few animal...

Haou-Tzong Ma, 馬豪聰

博士 / 中國醫藥大學 / 癌症生物與藥物研發博士學位學程 / 103

Chieh-Ying Chiang, 江倢瑩

碩士 / 中山醫學大學 / 應用化學系碩士班 / 102 / Alginate(Alg)，which is commonly isolated from brown algae，is an anionic linear polysaccharide composed of two saccharides: epimeric β-D-mannuronate (M) and α-L-guluronate (G). The M and G monomers are covalently bonded through 1,4-glycosidic linkages and arranged into either homopolymeric blocks (MM and GG) or alternating blocks (MGMG) along the polymeric backbone. According to the “egg-box” model，two facing GG blocks can be coordinated with divalent Ca2+ ions, res...

Wei-Jyun Lian, 連偉竣

碩士 / 國立雲林科技大學 / 化學工程與材料工程系 / 103 / In recent years, in situ tissue engineering is a novel technology that the growth factor is added into scaffold before implantation. The chemokine stromal cell-derived factor-1 (SDF-1) has been shown to recruit and mobilize bone marrow stromal cells to the site of injury and is suggested to be a promising candidate for bone tissue engineering. Strontium, a trace element in calcified tissue, plays a crucial role in bone mineralization and remodeling. In this stu...

Chiou, hsiu-hui, 邱秀慧

碩士 / 國立臺灣大學 / 海洋研究所 / 86 /

Chen, Shu-Jyun, 陳書竣

碩士 / 國立臺灣海洋大學 / 食品科學系 / 103 / 5-Fluorouracil (5-FU) is widely used in the treatment of cancer, and fucoidan can function as an adjuvant to regulate immune cells such as enhancing cell maturation and inducing immune responses. In this study, we developed 5-FU coated chitosan/fucoidan (CS/F) functional nanoparticles (NPs), which are expected as a desired treatment to combine immunotherapy with chemotherapy for cancer treatment. As the CS to F weight ratio was 5:1, the NP shows the best performance...

Wan-Ting Kao, 高婉婷

碩士 / 國立中興大學 / 環境工程學系所 / 100 / Microalgae biomass is a potential substrate for bioethanol production because (1) microalgae can exhibit a higher productivity than lignocellulosic crops; (2) cultivation of microalgae can avoid the competition between energy production and food supply when using energy crops; (3) microalgae, compared with terrestrial plants, have a low lignin content to facilitate the lignin removal. In order to generate bioethanol from microalgae biomass, cellulose in the microalg...

黃振平

碩士 / 國立臺灣大學 / 漁業科學研究所 / 87 / Purple clam is a kind of shellfish of high economic importance in Taiwan. Since the paralytic shellfish poisoning (PSP) incidents due to the ingestion of contaminated purple clam occured in 1986 and 1991, the consumption and aquaculture industry of purple clam has been declining. According to the long term survey of toxic shellfish monitoring, it was noted that most of the toxic cases were related to one of the purple clam species, Hiatula rostrata, but none to H. di...

萬英群

碩士 / 國立屏東科技大學 / 動物科學與畜產系所 / 100 / Spirulina (Arthrospira spp., commonly known as cyanobacteria) is an edible alga, which is rich in proteins, essential amino acids, essential fatty acids, vitamins and minerals, and other nutrients. The Spirulina contains a special pigment carrying protein (C-phycocyanin, CPC). The CPC has effects on antioxidation, reduces liver lipid peroxidation, antibacteria, antitumor and immunoregulation, which could be ideal materials for health food. In this current paper,...

Shu-lin Cheng, 鄭淑玲

碩士 / 國立中山大學 / 企業管理學系研究所 / 96 / Ever since China opened its door to the world in the ‘90, investors poured into China to “dig gold”. Hence, a new surge of “learning Chinese” has been setting off. According to an unofficial statistics, over 30 million people all over the world are learning Chinese. Likewise, Taiwan is certainly eager to attract Chinese learners while the whole world is infected with “Chinese fever”. As a result, Chinese centers affiliated to higher education institutes in Taiwan...

Tse-Zung Liao, 廖澤蓉

碩士 / 元培科技大學 / 生物技術研究所 / 98 / High incidence rates of cancer in recent years, patient that caused discomfort on side effects and damage of normal cells for radiotherapy. Many researches indicated that the natural antioxidants provided free radicals scavenging ability, antioxidant activity and anti-cancer effect. The radio-protective and radio-sensitivity of catechin, EGCG, gallic acid and 4 samples of purple bud tea ( PTE ), black tea ( TTES No. 8, BTE ), oolong tea ( TTES No.12, MTE ), Spirulin...

Sheng-wen Wang, 王聖文

碩士 / 國立中山大學 / 海洋生物研究所 / 96 / The photoprotect compound mycosporine-like amino acids ( MAAs ) are a family of secondary metabolites can protect organisms exposed to solar ultraviolet radiation ( UVR ), only produced in the algae. Analyzed by HPLC, only Alexandrium minutum Halim ( Dinophyceae ) produces MAAs among seven marine microalgae species. The strains named AM-2、AM-3、AM-4 and AM-6 showed the positive results for producing the MAAs, but not AM-5. Researches on the stability the MAAs extracts...

Yi-Chen Shen, 沈宜臻

碩士 / 國立中興大學 / 化學工程學系所 / 99 / This work investigated column elution chromatography of ultrasonic solvent extracts coupled with supercritical anti-solvent precipitation of carotenoids-containing particulates from microalgal Dunaliella Salina species. The extract contained carotenoids ranged from 61.3mg to 72.5mg using ultrasonic stirred ethyl ether or tetrahydrofuran (THF) extraction. When 6L ethyl alcohol was used in a reverse-phase column to elute 2g of the ethyl ether extract, the purity of tra...

Chei, Chen-Yu, 蔡鎮宇

碩士 / 國立屏東科技大學 / 水產養殖系所 / 104 / The probiotic effects of Bacillus subtilis E20-supplemented diets (control (without probiotics), 108, 109, and 1010 cfu g-1, respectively designated C, E108, E109, and E1010) fed to the grouper, Epinephelus moara, for 56 days were evaluated by tracking the growth performance, non-specific immune responses, and disease resistance against Vibrio alginolyticus. No significant differences in survival of fish were found among groups during the experiment. Growth enhance...

Yang, Yen-Tung, 楊晏東

碩士 / 國立清華大學 / 生醫工程與環境科學系 / 104 / Photoelectrocatalysis which combines photocatalytic and electrocatalytic processes, is not only a novel and feasible method for degradation of Microcystin-LR, but also a powerful method to enhance the photocatalytic activity of photocatalyst. In this study, we have been successfully fabricated zero-valent copper/titanate nanotubes/graphene composite (Cu0/TNT/rGO) and apply to photoelectrocatalytic degradation of Microcystin-LR. At first, we can obtain the titan...

Tsu-Chun Weng, 翁祖駿

碩士 / 國立中央大學 / 生物醫學工程研究所 / 104 / Normally biogas contains ~40% CO2 and it may severely hinder the combustion efficiency of biogas and cause greenhouse effect accordingly. Microalgae have been known to be able to recycle/metabolize CO2 through photosynthesis and produce abundance of chemicals/biologicals in form of biomass and/or biolipids, showing a high potential for use in CO2 removal for biogas purification. However, the amount of CO2 given to microalgae has to be adequately controlled to avoi...

Kang, Chia Wei, 康家瑋

碩士 / 國立清華大學 / 工程與系統科學系 / 104 / Cancer is the first leading cause of death in developed countries and the second one in developing countries, accounting for around 13% of all deaths in 2008. Conventional cancer therapy, chemotherapy, the agents are distributed non-specifically where they affect both normal and cancerous cells. Nanoparticles drug delivery carriers, by using both passive and active targeting strategies, can enhance the intracellular concentration of drugs in cancer cells while avoi...

YUAN-CHIEH HSIEH, 謝元傑

碩士 / 國立臺灣科技大學 / 化學工程系 / 104 / Cyanophycin is a nonribosomally synthesized biopolymer, and was originally discovered in cyanobacteria about 100 years ago. The structure of natural cyanophycin consists of aspartic acid and arginine, and is arranged as an aspartic acid backbone with an arginine side chain which is linked to the β-carboxyl group of each aspartate by its α-amino group. The gene transformed into microorganisms can be used to produce large amounts of cyanophycin. In this study, we ...

JYUN-YIN SIAO, 蕭竣尹

碩士 / 國立臺灣科技大學 / 化學工程系 / 104 / In recent years, self-assembled monolayer (SAM) technology has found a wide variety of applications in both nanotechnology and biomedical fields. In this study , we used two kinds of sources to prepare self-assembled films. One is the combination of hyaluronic acid and insoluble cyanophycin, which was obtained by cultivating Escherichia coli carrying BL21-CodonPlus (DE3)-RIL with Synechocystis sp. PCC6803 cphA. The other on...

Liu, Ho-Chang, 劉和昌

碩士 / 國立臺灣海洋大學 / 機械與機電工程學系 / 104 / This theis, use of microfluidic device with optical detection system to measure oxygen variation of Chlorella under different environmental conditions. The microfluidic chip device can makesurements the surrounding oxygen consumption (production) rate, for understand the impact of health chlorella, from different concentrations of herbicide (Diuron) . Herbicide (Diuron) ,for inhibitors the photosynthetic electron transport chain , it will not affect the role of ...

Ming-Kai Liu, 劉明凱

碩士 / 國立成功大學 / 工程科學系碩博士班 / 96 / This study describes the optimal formation of microemulsions with Taguchi method and the generation of monodisperse calcium alginate (Ca-alginate) microcapsules on a microfluidic platform using the commercial optical disc process. Our strategy is based on combining the injection molding process for cross-junction microchannel with the sheath focusing effect to form uniform water-in-oil (w/o) emulsions. These emulsions, consisting of 1.5% (w/v) sodium alginate (Na-a...

Tsai,Cheng-Shun, 蔡承順

碩士 / 國立中正大學 / 化學暨生物化學研究所 / 104 / In recent years, there are many research about green algae Chlorella pyrenoidosa and its extracts. How to extract bioactive proteins efficiently from green algae and to know their physic-chemical characteristics play an important role on drug develop and food industry. In this study, we extracted two major proteins from commercial green algae Chlorella pyrenoidosa. After purifying, we analyzed their qualitative properties and investigated their bioactivity on RAW...

CAI, PENG-CHENG, 蔡朋呈

碩士 / 南臺科技大學 / 生物科技系 / 105

Wei-TingHuang, 黃暐庭

碩士 / 國立成功大學 / 環境工程學系 / 104 / Effect of TiO2 and H2O2 on Microcystis aeruginosa and the degradation of microcystins (MCs) under UV-LED light illumination was investigated. The study is divided into two parts. In the first part, the powder TiO2 was used as photocatalyst, and it is one of the sources to produce hydroxyl radical (･OH). As the powder TiO2 may block the light penetration into deeper water, higher dose of TiO2 applied did not lead to higher production of ･OH. Four main factors namely T...

Fang-YuWeng, 翁芳瑜

碩士 / 國立成功大學 / 化學工程學系 / 104 / In this study, rapid and direct Raman analysis of microalgae was proposed and demonstrated through microwells-assisted filtration using anodic aluminum oxide (AAO) membrane. Instead of evaporation, a droplet of algal solution was dispensed in the polydimethylsiloxane (PDMS) microwells, which was placed on top of the AAO membrane, followed by applying the vacuum. The algal cells deposited inside the microwells and were subsequently analyzed by Raman spectroscopy. The ...

Jian-Ren Chen, 陳建任

碩士 / 國立中興大學 / 化學工程學系所 / 101 / In this study, supercritical anti-solvent pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-β-carotenes. Total concentration of 9-cis (134.7) and trans-β-carotene (204.2) increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for cis and 520.8 for trans) of the elution fraction by 82% ethanol. Supercritical anti-solvent (SAS) method produced particulates containing 932.1 mg/g (355.6 for cis and ...

Pei-San Lee, 李佩珊

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 93 / Distorted DNA helical structures induced by covalent chemical adducts or UV-irradiation are removed by specific DNA repair pathways and these repair systems are initiated by the binding of damage-recognition protein to DNA lesions. Distorted DNA-binding proteins in lower plants are studied in this thesis by electrophoresis mobility shift assay (EMSA), affinity adsorption, 2-D gle electrophoresis and mass spectrometric analysis using the unicellular alga Chlorella p...

Ching Hsin Chiang, 江靜欣

碩士 / 國立臺灣海洋大學 / 食品科學系 / 93 / Blooms of the toxic, paralytic shellfish poisoning (PSP), chain-forming dinoflagellate Gymnodinium catenatum Graham pose a serious risk to human health, aquaculture developments and the coastal environment. G. catenatum was described for the fist time in Gulf of California, Mexico by Graham (1943). During the last 20 years, G. catenatum has been found in an increasing number of locations worldwide, e.g. Spain, Mexico, Japan, Portugal, Philippines, Uruguay, Morocco, A...

Po-Nien Lin, 林柏年

碩士 / 國立中興大學 / 化學工程學系所 / 94 / Base on the donors of articular chondrocytes are limited and hard to expand. To obtain adequate chondrocytes and keep acitivity for autologous chondrocytes implantation or tissue engineering research is very important. Expansion by monolayer culture is mostly used. It is well known that chondrocytes cultured with low seeding density and passaged in monolayer will dedifferentiate. Low seeding density can greatly increase the total cell number with less passaging. If ...

Chin Shan Shih, 施金杉

碩士 / 東海大學 / 化學工程與材料工程學系 / 101 / Due to global warming and the depletion of energy, the applications of microalgae get much attention. Microalgae are autotrophic microorganismshaving extremely high photosynthetic efficiency and arevalued as rich source of lipids,it also has great impact on water purifying. In this study, we investigate the following aspect by cultivating the microalgae: (1) Investigate the impact on the concentration ofChlorella vulgaris EXP-06by using flat-plate photobioreactor w...

Wan-JuChang, 張婉茹

碩士 / 國立成功大學 / 化學工程學系碩博士班 / 100 / Sodium alginate is a water-soluble natural biopolymer. Because of its nontoxicity, biodegradability and biocompatibility, it has been approved by FDA. In this study, we prepared a magnetite-sodium alginate/polyethylene oxide (Fe3O4-SA/PEO) nanofibrous mats. Fe3O4 were synthesized by chemical co-precipitation technique and loaded into sodium alginate-based nanofibers which have been electrospun by blending with a biocompatible, synthetic polymer PEO. To enhance hy...

劉庭儒

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / Chemical factors and stem cells are important elements in tissue engineering. When tissue is damaged, specific chemokines are secreted to attract stem cells homing for tissue repair. Stromal cell derived factor-1 (SDF-1), massively released in injured tissue, affect the behaviors of stem cells and the effect of tissue repair. However, two drawbacks, short half-life and easily degraded by enzymes, limit the application of SDF-1. The aim of this study is to release SDF...

Ya-Ting Yang, 楊雅婷

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / In tissue engineering, growth factors and cells are important elements for repairing nerve injuries. Supplemented with exogenous growth factors is an effective medical strategy for protecting nerve injury and promoting nerve regeneration. It has been demonstrated that basic fibroblast growth factors have great effect on angiogenesis and neuronal cell survival; however, bFGF has two shortages: short half-life and easily degraded by enzymes. Therefore, the aim of this ...

Yu-Ting Chiu, 邱郁婷

碩士 / 國立中興大學 / 食品暨應用生物科技學系所 / 98 / Fucoxanthin is the major carotenoid in brown sea algae and has been shown to exert antioxidant activity. However, Fucoxanthin is highly unstable and it may attack by nucleophile molecule to cause the pro-oxidative actions because of its unique chemical structure including 5, 6-monoepoxide. Thus, the purpose of this research was to determine whether fucoxanthin activates cellular antioxidant enzyme expression via up-regulation of Nrf2/ antioxidant-response elem...

Yu-Lu Tseng, 曾宇璐

碩士 / 國立中山大學 / 海洋生物研究所 / 99 / Methionine sulfoxide reductase A (MSRA) and MSRB are responsible for the repairing of methionine-R-sulfoxide (Met-S-SO) and methionine-S-sulfoxide (Met-R-SO) back to me-thionine, respectively. Five MSRA isoforms and four MSRB isoforms are discovered in the unicellular green alga Chlamydomonas reinhardtii. Whether high light regulates CrMSR ex-pression via photosynthetic electron transport (PET) was examined. By checking the se-quence of PCR product of each isoform, q...

Liang-Chi Wang, 汪良奇

博士 / 國立臺灣大學 / 生態學與演化生物學研究所 / 99 / The pollen and diatom preserved in lacustrine sediment are the good indicators for reconstructing the paleo-environment. The successions of pollen and diatom assemblages respectively infer to the vegetation history and aquatic condition as well as elaborate the climate changes. In order to reconstruct the nature history of East Taiwan, the sediments from tree lakes were used namely Liyu Lake of Hualien County, Tsuifong Lake of Ilan County and Dahu Lake of Ilan C...

Peng, Yu-Wen, 彭雨文

碩士 / 國立中興大學 / 植物學系 / 85 / Desiccation-tolerant Chlorella sp. strain DT(from an arid mountainous area of Taiwan) and Desiccation-sensitive Chlorella pyrenoidosa (211-8b, from the AlgaeCollection Center, Univ, Gottingen, Germany) were cultured Chlorella-medium.Only a limited amount of water can be conserved in the DT and 8b cell when dehydrate using an infra-red radiation balance at 42oC for 3 h, and it tooko...

Shin-Shiung cheng, 程信雄

碩士 / 大葉大學 / 環境工程學系碩士班 / 94 / Continuous cultivations in a light/dark dual-tank photobioreactor were conducted to study the effects of culture conditions on the growth of Tetraselmis chui using sodium bicarbonates as the carbon sources. The solution of sodium bicarbonate was used to simulate the alkaline solution after absorbing carbon dixide. The main results and conclusion are as the following： (1) The effects of the concentrations of sodium bicarbonate on the growth of Tetraselmis chui During...

Ting Chang-Kai, 丁中凱

碩士 / 國立雲林科技大學 / 環境與安全工程技術研究所 / 86 / The purpose of this research is to conduct feasibility study on the use of the aerobic conditions for wastewater denitrification treatment with immobilized support of calcium alginate, and to evaluate the influence on micro-porosity, activity and mechanical strength by the additives including polyethylene glycol (PEG) and polyethylene imine (PEI) on the calcium alginate support, in order to screen the method and material for immobilized support with potential ...

Liu Tzu-Ling, 劉芝伶

碩士 / 台北醫學院 / 細胞及分子生物研究所 / 86 / Infantile spasm (IS), is a form of epilepsy specifically occurring at infant s tage, is refractory to conventional anticonvulsant treatment but is exceptiona lly sensitive to adrenocorticotropic hormone (ACTH) treatment. Corticotropin- releasing hormone (CRH), a major regulatory factor of ACTH secretion in pituit ary, has been implicated as an important neuro-hormone participating in seizur e generat...

白松穎

碩士 / 國立清華大學 / 生物資訊與結構生物研究所 / 95 / When cell exposed to extreme environment, both their physiology and morphology change accordingly. In previous studies, we found that Chlorella pyrenoidosa exposed to heat stress (46.5℃) for one hour and cultured again under light or dark environment., their nuclear DNA would be condensed. If recultured under light, both chlorophylls and DNA would degrade. We thus speculated that heat stress plus readtirition might lead chlorella to go through a process of progr...