Liang-Yu Hou, 侯亮瑜

碩士 / 國立臺灣大學 / 化學工程學研究所 / 105 / We have proposed a novel DSEC (direct saponification-esterification conversion) strategy that could directly disrupt cells and convert lipid into biodiesel efficiently in a single vessel under the conditions of high water and fatty acid. It is advantageous at least for several reasons. First of all, DSEC eliminates lipid extraction and purification steps in the conventional process, which usually accounts for more than half of production cost. Secondly, compared wi...

Yi-Chun Yao, 姚怡君

碩士 / 國立臺灣大學 / 生物環境系統工程學研究所 / 105 / Agricultural Wastes due to the sources of scattered, lack of centralized collection and recycling mechanism is not perfect, generally more in the origin of the direct pile, composting or burning, etc., in order to solve the shortage of agricultural waste recycling and decomposition surplus of material problems remaining, enlarging the volume of leaching fallen leaves, rice straw and tea residue with distilled water by the ultraviolet disinfection sterilization ...

Hsueh-Ting Wang, 王雪婷

博士 / 國立臺灣大學 / 食品科技研究所 / 105 / Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structu...

Chen, Cheng-Li, 陳政莉

碩士 / 慈濟科技大學 / 放射醫學科學研究所 / 105 / Abstract The purpose of this study was to investigate the healing potential and possible mechanism of enteromorpha prolifera extraction (EE) and enteromorpha prolifera polysaccharides (EP) on the wounds treated with UV irradiation. In this study, human foreskin fibroblast cells (HS-68) were used to simulate wound healing. The experimental conditions were divided into six groups, including control group, enteromorpha prolifera extraction treatment group, enterom...

WANG, DONG-SHENG, 王東生

碩士 / 國立臺南大學 / 生物科技學系碩士班 / 106 / This thesis comprises two parts to study two proteins responsible for redox reactions: The first part describes the biotransformation of a commercially available rude extract of soy isoflavones by recombinant Escherichia coli expressing a tyrosinase from Bacillus megaterium. The original crude soy extracts contains significant amounts of the isoflavone glycosides daidzin and genistin. After biotransformation, two major products were isolated and identified as 3'-h...

CHENG, CHIA-HO, 鄭佳和

碩士 / 大仁科技大學 / 製藥科技研究所 / 106 / Fucoidan, which had various biological activity, was found mainly in various species of brown seaweed and have been found in animal species, including the sea cucumber. Based on health belief model (HBM), this study aimed to explore and clarify the adults’ fucoidan buying behaviors. In this study, 379 convenient samples around Taiwan were solicited, and then data were analyzed by using descriptive statistics, dependent samples t-test, independent samples t-test, chi...

CHEN, TZU-LIN, 陳姿陵

碩士 / 弘光科技大學 / 化妝品應用研究所 / 106 / Ultraviolet (UV) light is ubiquitous in our environment. Excessive absorption of UV will generate free radicals that cause injury or inflammation of the skin and scalp, and lead to aging or even cancer. The main purpose of this study was to investigate the antioxidative effects of extract from Chlorella pyrenoidosa and to investigate the effects of the extract on the growth of mouse fibroblast (3T3) and human hair follicle dermal papilla cells (HFDPC) and the mecha...

Chien, Ya-Yun, 錢亞筠

碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 105 / Diatoms, a kind of single-celled algae, are the dominant phytoplanktons in the ocean. Each diatom has a cell wall, also known as frustule, which is made of biosilica (SiO2) and bearing the species-specific pattern. Based on the pattern of fructule, diatoms may be denoted as Coscinodiscophyceae and Pennales. Currently, the study of fructule formation was focused on Coscinodiscophyceae, especially in Thalassiosira pseudonana. Some proteins isolated from fructule...

Hsiao-Cheng Cho, 卓筱錚

碩士 / 國立臺灣海洋大學 / 食品科學系 / 104 / Dengue virus (DENV) is a mosquito-borne pathogen that infects over 50 million people every year worldwide, causing hemorrhage and even mortality. Several bioactivities have been described for brown seaweed, including antiviral activities, anti-inflammatory properties and enhancement of immune responses. Therefore, the purpose of this study is to assess the protective effect of Sargassum hemiphyllum extracts on dengue virus type II (DENV2) infection. The yield of Sar...

Lee, Yu-Ru, 李育儒

博士 / 國立屏東科技大學 / 生物資源研究所 / 105

Chen, Hung-Yu, 陳竑宇

碩士 / 國立清華大學 / 工程與系統科學系 / 105 / Since 1980s, the average of global temperature increased year by year due to burning lots of fossil fuel caused a huge amount of carbon dioxide emission. Carbon capture and storage system (CCS) was developed in order to decrease the amount of carbon dioxide in atmosphere, yet its operation cost was high. Therefore, we can utilize plants doing photosynthesis to absorb the carbon dioxide in atmosphere. Microalgae have greater photosynthesis efficiency and growing rat...

LIN, AN-JIE, 林安婕

碩士 / 中臺科技大學 / 醫學影像暨放射科學系暨研究所 / 106 / In the large scale production of microalgae biodiesel, high biomass productivity and high the lipid content of microalgae is the key. In order to obtain such microalgae, the microalgae of FGA326S (Secnedesmus) isolated from our laboratory was induced by ultraviolet radiation and cultured in a two-stage manner, and the lipids distribution of microalgae was observed by Nile red fluorescence staining technique and microscope equipment, and the lipid content was ...

Keng-HsienLin, 林耿賢

碩士 / 國立成功大學 / 化學工程學系 / 105

王政中 WANG, JANG-JUNG, 王政中

碩士 / 國立高雄應用科技大學 / 化學工程與材料工程系博碩士班 / 106 / Hydroxyapatite (HAp) is a kind of calcium phosphate similar to the human bone apatite. HAp has been attracted great attention in scientific community because it has a wide application in bone tissue engineering, remineralizing agent, drug delivery and bioseparation. HAp is also a good adsorbent for purifying biomolecules due to the constitution of calcium, phosphate and hydroxyl groups. HAp was reported to recover phycobiliprotiens, however, the report r...

LEE,JIN-JIE, 李進傑

碩士 / 國立高雄海洋科技大學 / 水產養殖研究所 / 106 / The cnidarians form the amazing coral reef ecosystem mutually with zooxanthellae, its foundation was based on the intracellular symbiosis. By endosymbiosis, zooxanthellae were capable of living in cnidarian host gastrodermal endoderm cells with large numbers. Traditionally, the pH of symbiosomal lumen was thought to be 6-7. However, a recently report by utilizing pH indicators indicated that the pH of symbiosomal lumen in coral cells is below 4, revealing that t...

Li, Wei-Ming, 李偉銘

碩士 / 國立臺灣海洋大學 / 食品科學系 / 106

Chen, Yu-Ting, 陳宇庭

碩士 / 國立臺灣海洋大學 / 食品科學系 / 106 / Radiation-induced fibrosis (RIF) is one of the most common late complications of radiation therapy. This study was aimed to evaluate the possible protective effects of Low molecular weight fucoidan (LMF) against γ-radiation induced damage in vitro. It was observed that the exposure to γ-radiation dose-dependently caused a significant decrease in the cell viability. The pre-treatment and pos-treatment of LMF attenuated 1 Gy cytotoxicity. In addition, LMF also reduce ...

Chia-Jung Hsieh, 謝佳蓉

碩士 / 東海大學 / 生命科學系 / 102 / Cyanidiales is a group of unicellular red algae that can inhibit in the low pH and high temperature environments. Cyanidiales consist of three genera and seven species, namely Cyanidium caldarium, Cyanidioschyzon merolae, Galdieria sulphuraria, G. maxima, G. partita, G. daedala, and G. phlegrea. The biodiversity of Cyanidiales has been well investigated in the volcanic area of central Italy and the Yellowstone National Park of USA by using both environmental sequencing ...

Hu, Xiao-Lan, 胡筱嵐

碩士 / 國立臺灣海洋大學 / 輪機工程學系 / 107 / Microalgae have high oil content. The process of cultivating microalgae can help maintain the biological system of aquatic cycle, while reduce the impact of land use, food competition and seasonality. In order to improve the stability of the algae cultivation process, the fungus and the microalgae are mixed to adjust the growth conditions of the metabolites and the medium composition in the water, so as to promote the propagation of the algae and rapidly increase t...

Cheng, Yu-Heng, 鄭宇恆

碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 107 / According to the patterns of frustule, diatoms can be roughly divided into two groups, centric diatoms and pinnate diatoms. Currently, the biomineralization process of frustule has been studied on Thalassiosira pseudonana, a centric diatom. Several proteins were identified as frustule synthesizing components, such as silaffins (silica affinity proteins). There are 4 silaffins were identified in T. pseudonana. The overall sequence identity of silaffins among di...

Yu-Bin Wang, 王譽賓

碩士 / 國立中興大學 / 化學工程學系所 / 94 / In this study, we focused on skin tissue engineering. We combined alginate and chitosan in preparing the 3D scaffold, and measured the growth of human gingival fibroblasts and human skin fibroblasts in the scaffold. On the other hand, we modified chitosan by incorporating 5 nm gold nanoparticles、silver nanoparticles and FGF1, tested the fibroblasts growth and extra-cellular matrix secretion such as Type I collagen content and GAGs on the films. We also analyzed the a...

Chun-Hung Chang, 張俊鴻

碩士 / 國立中央大學 / 環境工程研究所碩士在職專班-環境工程規劃 / 94 / ABSTRACT Ethylenediaminetetraacetic acid (EDTA) has been used to extract heavy metals from contaminated soils; however, EDTA is not biodegradable and the treated soils might not be suitable for various uses. The objective of this thesis is to study the feasibility of using biodegradable sodium alginate as an extraction agent. The contaminated soil obtained from Taipei contained arsenic of 8089 mg/kg soil, and that obtained from Tainan contained chromi...

YU LEE, 李昱

碩士 / 國立高雄應用科技大學 / 化學工程與材料工程系博碩士班 / 104

Zhang,Yong-Qing, 張詠晴

碩士 / 國立中正大學 / 化學暨生物化學研究所 / 105 / Dunaliella salina is an algae rich in natural carotenoids. This study was focused on the anti-oxidation activity and inhibition of tyrosinase activity of Dunaliella salina . In the first part, the carotenoids in the Dunaliella salina were isolated and purified as main components as lutein, zeaxanthin, α-carotene, trans-β-carotene and 9-cis-β-carotene by high performance liquid chromatography. The 9-cis-β-carotene was the most abundant part of carotenoids in Dunal...

Tsung-Hua Hsieh, 謝宗樺

碩士 / 國立臺東大學 / 生命科學系碩士班 / 104 / In recent years, water eutrophication and cyanobacteria blooms have been serious problems influencing the landscape and ecological functions of water bodies. How to control cyanobacteria blooms and water eutrophication efficiently is recognized as vanguard and hot-spot issue in the field of water environment research. The inhibitory allelopathy of plants on harmful algae growth have been receiving world-wide concern recently. In this study, inhibitory effects of Ba...

Cheng-Hsien Lin, 林政賢

碩士 / 國立中央大學 / 化學工程與材料工程學系 / 104 / Although electrospun alginate scaffolds can be used to enrich cancer cells with high drug resistance, the low cell adhesion and proliferation restricts its application. Therefore, we incorporated polycaprolactone (PCL) for coelectrospinning to prepare composite nanofibrous scaffolds. The composition of nanofibers can be regulated through adjusting the perfusion rates of polymer jets. These composite scaffolds were applied to culture cancer cell lines. The enrich...

Ying-Ru Fang, 方盈茹

碩士 / 國立臺灣大學 / 化學工程學研究所 / 104 / Microalgae has become a potential resource of biodiesel in recent years for its fast growth, non-food source, and CO2 capture ability. However, efficient and economic production of biodiesel from microalgae is still a challenge. In this study, we presented a novel strategy of direct saponification-esterification conversion (DSEC) from wet microalgae (Chlorella vulgaris) to biodiesel. The process was accomplished with two consecutive additions into wet algae. With t...

Chi-Wen Chen, 陳琦雯

碩士 / 大葉大學 / 生物產業科技學系 / 101 / Due to the quick development of human activities and consumption of fossil fuels, the energy crisis and environmental pollution associated problems are a major concern of society. Algae have been proposed as a potential renewable fuel source was compared with the conventional oil crops, microalgae are more attractive as feedstock for biodiesel production, due to their high lipid content and photosynthesis efficiency. The apparent benefits of combining microalgal biod...

Wen-MinLee, 李文敏

碩士 / 國立成功大學 / 化學工程學系碩博士班 / 99

Chia-Jung Li, 李佳蓉

碩士 / 國立東華大學 / 海洋生物科技研究所 / 98 / Marine invertebrates often form the obligate symbiosis with microalgae Symbiodinium, such as the endosymbiosis during the Cnidaria-Symbiodinium association. The cellular and molecular mechanism of the endosymbiosis remains unclear. The aim of the present study is to study how the division or Symbiosinium in endosymbiosis is regulated by examining the role of cAMP in cell cycle regulation. Results showed that dynamic level of intracellular cAMP correlated with cell ...

Wang, Wen-Mei, 王文美

碩士 / 國立交通大學 / 環境工程系所 / 99 / Low turbidity along with abundant algae is frequently found in raw water taken from reservoirs by water treatment plants (WTPs). Because the density of the algae is too low to be effectively removed from the water by coagulation-sedimentation, the burden on the operation of the filtration unit in the WTP is significantly increased. In addition, the algae may clog the filter and slow down or stop the filtration. While a hybrid pre-oxidation/coagulation-sedimentation pr...

Chao-Chin, 胡超群

博士 / 中山醫學大學 / 生化暨生物科技研究所 / 96 / Functional foods can be defined as the foods contains special integrants which can improve the health condition and modulate biological function in human. The special benefit integrants in functional food verifies as the cultivated environment, process of extraction and restore. The goal of this research is to develop a dependable method to separate and determine the bioactive integrants in functional food. During recent years, steroidal saponins have attracted a ...

CHEN, YAN-CHANG, 陳衍昌

博士 / 國立臺灣大學 / 海洋研究所 / 81 /

Yun Yeh, 葉芸

碩士 / 國立臺灣大學 / 化學工程學研究所 / 102 / Microalgae, a photosynthetic organism, has the ability of fast-growing and lipid accumulating, which makes it a highly potential source of biofuel. However, there are some current technical barriers, such as high energy and organic solvent consumption for harvest, drying and lipid extraction. To make the process of producing biofuel production from microalgae economically feasible, an innovative process was designed to convert lipid inside microalgae into biodiesel...

Mei-YiKuo, 郭美怡

碩士 / 國立成功大學 / 化學工程學系 / 102 / In this study, a continuous flow dielectrophoresis (DEP) microfluidic chip was fabricated and utilized to sort out the microalgae (C. vulgaris) with different lipid contents. The proposed separation scheme is to allow the microalgae with different lipid contents experience different negative or no DEP force at the separation electrode pair under the pressure-driven flow. The microalgae which experience stronger negative DEP will be directed to the side channel while ...

Wen-Chen Chuang, 莊文貞

博士 / 國立中興大學 / 獸醫學系暨研究所 / 103 / part 1 Dunaliella salina (D. salina) has been shown to have antioxidant property and induce apoptotic cell death of human cancer cell in vitro. However, there is no information available on anti-leukemia effect or immunomodulatory activity in vivo. In this study, we applied D. salina to syngeneic leukemia-implanted mice (BALB/c and WEHI-3) to investigate its immunological and anti-leukemia properties. Oral administration of D. salina (184.5, 369 and 922.5 mg/kg) fo...

You-Liang Zeng, 曾右良

碩士 / 大仁科技大學 / 環境管理研究所 / 102 / Microalgae, which have a higher biomass production and a faster growth rate than other energy crops, has widely recognized as the feedstock for third-generation of biofuels. Many microalgae are exceedingly rich in lipid content for producing biodiesel. However, the accumulation of lipid and increase of cell growth rate depend on diverse factors, including cultivation conditions (photoautotrophic, heterotrophic, mixotrophic and photoheterotrophic cultivation), algal ...

Hsu, Chichuang, 許啟詮

碩士 / 東海大學 / 環境科學與工程學系 / 101 / The objective of this study is to use immobilized Laccase beads made from calcium alginate to degrade emerging contaminants in water, including ibuprofen, naproxen, indigo, and dimethylphenol. Results indicate that beads having the lowest Laccase leakage rate were observed when made from 2 % of alginate together with 3 % calcium chloride. In batch experiments, the BCRC Laccase was found to be ineffective in degrading ibuprofen, naproxen, and indigo. However, with t...

Chen, Lijuan, 陳儷娟

碩士 / 東海大學 / 化學工程與材料工程學系 / 101 / During cultivation, Rhodotorula glutinis utilized C sorce and released CO2. Moreover, organic acids were synthesized and the pH of culture dropped. As a result, the growth of yeast was inhibited. Scenedesmus obliquus utilized CO2 and released O2 during photosynthesis. The cells growth of R. glutinis and S. obliquus would be raised with light exposed. The metabolites are inhibiative to R. glutinis and S. obliquus in the medium were removed and it was sutable to gro...

Yi-Ru Chen, 陳怡如

碩士 / 淡江大學 / 水資源及環境工程學系碩士班 / 99 / The high concentration nitrogen wastewater supplies microalgae with enough nutrient and suitable environment when using the microalgae treated the wastewater. The microalgae is provided with the characteristics of reproduce rapidly and quick adaptation, which have high efficiency to treat high carbon and high nitrogen concentration of wastewater. The microalgae would be a new clean energy source in this energy crisis days. In order to find the optimum parameters ...

Ching-hsiang Tsai, 蔡景翔

碩士 / 國立臺灣科技大學 / 化學工程系 / 98 / In 1887, cyanophycin is a non-ribosomal protein discovered by Borzi in cyanobacteria. Cyanophycin can be produced inside cyanobacteria. However, not all cyanobacteria are capable of producing cyanophycin. Cyanophycin is protein-like polymer which consists of equimolar amounts of aspartic acid and arginine arranged as a poly-aspartic acid backbone to which arginine residues are linked to the β-carboxyl group of each aspartate by its α-amino group. This study reports t...

Chang, Wei-Tung, 張為棟

碩士 / 東海大學 / 環境科學與工程學系 / 99 / Due to the climate change, the atmospheric CO2 is considered to be one of the main causes of global warming. Microalgae based wastewater treatment also releases CO2 but the microalgae consume more CO2 while growing than traditional wastewater treatment plants. The microalgae incorporated photobioreactor is a highly efficient biological system for converting CO2 into biomass. In this study, the packed tower with the sodium hydroxide solution and dairy wastewater was t...

Hsueh, Chiuchin, 薛秋琴

碩士 / 國立澎湖科技大學 / 食品科學研究所 / 99 / In this study, 156 bacteria have been isolated from soil and decayed brown algae. Strain P44 showed the strongest alginate lyase activity among isolates which can use alginate as sole carbon source. After gram staining, spore staining. catalase test, API 50CHB identification and 16S rRNA sequencing, P44 identified as Bacillus megaterium. Bacillus megaterium P44 can break down poy-1,4-D-mannuronate and poly-1,4-L-guluronate in the alginate polymer, produce protease ...

Yu-Ya Su, 蘇郁雅

碩士 / 淡江大學 / 水資源及環境工程學系碩士班 / 98 / Taiwan has been actively implementing the national sewage-based system, centralized sewage, reducing the amount of sewage discharged to rivers.It is the same way to cultivation of microalgae and sewage treatment system to provide a suitable environment and adequate nutrition source of biological purification mode of operation. It is replaced by cultivating microalgae to deal with sewage treatment, and it is not only improved the traditional sludge processing issu...

Hsi-Ju Hsueh, 薛希如

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 97 / Abstract The Order Gelidiales, consisting of a single family Gelidiaceae, comprises a dominant component of the marine flora in northern Taiwan. No taxonomic studies had been focusing on the Gelidiaceae from Taiwan, and the records of the Gelidiaceae were only seen in the previous marine floristic surveys of Taiwan. Therefore, a monograph of species diversity and systematic studies on Taiwan's Gelidiales based on morphological and molecular datasets is carried out...

Chun-Yi Wu, 吳駿逸

碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 / The purpose of this study was to investigate effects of the Spirulina platensis on blood glucose and lipid metabolism in type 2 diabetic rats. In this study, Wistar rats were separated into five groups as follows: (a) healthy rats fed on normal diet group (NA), (b) healthy rats fed on S. platensis diet group (NB), (c) type 2 diabetic rats fed on normal diet group (MA), (d) type 2 diabetic rats fed on S. platensis diet group (MB) and (e) type 2 diabetic rats fed on gl...

陳素蓮

碩士 / 台北醫學院 / 細胞及分子生物研究所 / 88 / Corticotropin-Release Hormone (CRH), a 41 amino acid neuropeptide, is a major regulator of the HPA axis, which release leads to pituitary production of adrenocorticotropic hormone (ACTH) followed by glucocorticoid secretion by the adrenal cortex. CRH has been implicated as an important neuro-hormone participating in seizure generation particularly in early life of both human and animals. It is possible that not only CRH presents as a pro-convulsant in the developi...

Reou-Ching Sheu, 許瑞卿

碩士 / 國立海洋大學 / 水產生物技術研究所 / 87 / Nucleotide excision repair (NER), a highly versatile DNA repair mechanism, is capable of removing various types of DNA damage including those induced by UV radiation and chemical mutagens.NER has been well characterized in yeast and mammalian systems but its presence in plants has not been well know in plants. NER in response to UV irradiation, cisplatin damageand mitomycin C (MMC) damage has been detected in soluble extracts of unicellular algae Chlorel...

Lu Ya Hui, 呂雅蕙

碩士 / 國立海洋大學 / 食品科學系 / 87 / Abstract The toxic dinoflagellate Alexandrium minutum was isolated from southern Taiwan and cultured in modified K medium. To find the best condition for cell growth and toxin production of A. minutum, some environmental and nutritional factors of modified K medium were modulated. It was found that the optimal environmental and nutritional conditions for those of A. minutum were as follows: temperature 25 oC, pH 7.5, light strength 6000 Lux, salinity 15 pp...

Yeh Yu-Ping, 葉玉萍

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / This study attempts to investigate the characteristics and pathogenicity of vibrio strains RGSW and RG3 isolated from disease fry of yellowfin seabream with a syndrome of red gut. RGSW and RG3 strains were identified to be Vibrio alginolyticus and V. harveyi according to a series of standard biochemical and physiological tests, and biochemical tests using API 20E and BIOLOG GN systems. RGSW strain was resistant to ampicillin、chloramphenicol、novobiocin、oxyte...