Liu chien-an, 劉健安

碩士 / 嘉南藥理科技大學 / 生物科技系暨研究所 / 95 / Two cysteine proteinase inhibitors, designated as CST-I and -II, were purified from the oocyte of crucian carp. The purification included acidification,CM-Sepharose FF, and Sephacryl S-100 HR chromatographs. The purification fold and recovery of CST-I were 98, and 22%, while those of CST-II were 112 and 20% respectively. The molecular masses (M) of CST-I and II estimated on SDS-PAGE were 14 kDa and 9.2 kDa. However, both inhibitors were shown to be about 14 kDa o...

Xie, Shu Yun, 謝淑雲

碩士 / 國立中興大學 / 食品科學研究所 / 82 /

pengyuching, 彭雨菁

碩士 / 國立中興大學 / 生命科學系 / 93 / The Minimata disease caused by mercury contamination during 1950s, and the Itai-itai disease caused by cadmium contamination during 1960s had made people pay more attention to the heavy metal pollution problems that may disturb the ecological environment of ocean and arise the toxic risks of human’s daily life. In the water environment, tilapia shows higher tolerance than other kinds of fishes and was known as a biological marker for heavily polluted water areas. Since...

Hsien-Wei Fan, 范憲維

碩士 / 國立中興大學 / 生命科學系 / 93 / The purpose of the present study is to examine the expression of branchial Na+,K+-ATPase (NKA) in milkfish (Chanos chanos) upon hyperosmotic challenge. The present biochemical and molecular studies including were performed to compare the milkfish (1) exposed to salinity of 0, 35, 60 ‰ environments for 4 weeks; (2) transferred from seawater (SW) to hypersaline water (HSW). The results revealed that: (1) in plasma, osmolality in fish of SW and HSW groups are higher than ...

Wen-yi Wu, 吳玟儀

碩士 / 國立中興大學 / 生命科學系 / 93 / Milkfish (Chanos chanos) is an euryhaline teleost which is able to survive in environment of salinities from 0‰ to 158‰. The kidney, like the gill, plays an important role in osmoregulation of euryhaline fish. The purpose of this study is to clarify the structures and localization of NKA in milkfish kidney, and to reveal renal NKA expression in milkfish adapted to water of different salinities (long-term experiment) and changes of renal NKA expression during FW- and HS...

You-ru Lin, 林宥汝

碩士 / 雲林科技大學 / 環境與安全工程系碩士班 / 96 / In this research, the biological toxicity test was performed on zebrafish(Danio Zenio) in gold nanoparticles solutions. The experimental results showed that different preparation method of solutions could result in different biological influences. The zebrafish was dead within 96 hours in the solution prepared via the seeding growth method containing CTAB (N-Cetyl-N,N,N-trimethylammonium bromide). However, the solution prepared via sodium citrate-tannin reduction...

Hsiu-Hua Ling, 凌秀華

碩士 / 國立彰化師範大學 / 生物技術研究所 / 96 / Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is one of the antioxidant enzymes known to directly reduce phospholipids hydroperoxides (PLOOH) within membranes and lipoproteins. We have cloned two isoform PHGPx, include PHGPx4A and PHGPx4B by RACE-PCR from zebrafish. The amino acid sequence of these two genes showed highly conserve with 66~88% similarity when compare with Homo sapiens, Rattus norvegicus, cow, Gallus gallus, Sus scrofal, Mus musculus, Dro...

Chin-Yu Cheng, 鄭進裕

碩士 / 國立彰化師範大學 / 生物技術研究所 / 96 / Copper (Cu) is an essential trace element for all organisms, but excess copper exposure can exert a variety of pathological effects in fish. In this study, we treated zebrafish embryos with 2.5-10 ppm either Cu+ or Cu ++ for 48h, survival rate was decreased and abnormal embryos was increased significantly. Lethal Concentration Fifty (LC50) was 5 ppm for either Cu+ or Cu++ treated. Reactive oxygen species (ROS) of embryos under copper ions treated were elevated by D...

Jin-Jiang Kao, 高錦江

碩士 / 國立臺灣大學 / 醫學工程學研究所 / 93 / In this research, we developed Microelectrode Array(MEA) chip and measured platform for zebrafish and zebrafish embryo ECG signals and other bio-potential signals .This platform also used in any MEA chip to measure any bio-potential signals. 　　MEA chip is fabricated by using Micro-Electro Machanical System(MEMS) processes. The substrate of MEA chip is glass, and the conduction-line-layer is used Ti(Titanium), Cr(Chromium), ITO(Indium Tin Oxide), and etc. The Bio-Con...

Li Chang, 張莉

碩士 / 國立臺灣大學 / 動物學研究研究所 / 93 / Nervous necrosis virus （NNV） is a critically important pathogen for reared marine fish, and has caused mass mortality in more than 30 fish species in recent 10 years. In this study, we cloned anti-NNV bacteria from the intestinal microflora of grouper, and investigated the possibility of using intestinal bacteria as a biological control for viral nervous necrosis（VNN） disease. The normal flora from grouper intestine was first isolated and preliminary identified by G...

Chen-Min Lin, 林晨敏

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 93 / Troponin I (TnI), a troponin component, inhibits the generation of acto-myosin and controls muscle contraction. Cardiac TnI is highly sensitive and specific markers of myocardial injury in acute coronary syndromes. Although troponin I genes have been identified in birds and mammals that encode the isoforms expressed in cardiac muscle, slow skeletal muscle and fast skeletal muscle, there is extremely little known about troponin I genes in lower vertebrates. In ord...

Chih-Wei Weng, 翁志偉

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 93 / Myf5 is the first expressed muscle regulatory factor in many species and the transcription mechanism is dedicated controlled. In zebrafish, myf5 is activated in presomitic mesoderm (PSM); prolong expression at newly forming somites and down-regulation in the elder one. However, the activation mechanism of myf5 in PSM has never been reported. Mesp family, including mespo, mesp-a, are expressed in PSM or somitomere that function as transcription factors. Here, we p...

Lin Ping-Chung, 林炳中

碩士 / 國立中興大學 / 資源管理研究所 / 86 / Aquaculture has been developed rapidly for several decays in Taiwan. It not only makes traditional farmers richer, but also earns lots of foreign exchange during 80''s. However, the development of aquaculture generates some environmental problems, such as land subsidence. Eel''s farming needs higher quality water, therefore, for keeping the good water quality, the eel''sfarmer must pump lots of groundwater. However, the shortage of groundwater has great important on ...

Chang, Chung-Kai, 張忠凱

碩士 / 國立中興大學 / 植物學系 / 86 / Abstract In the filamentous nitrogen-fixing cyanobacteria Anabaena sp. strain CH1 and CH2 a comparative study has been made on the GS protein synthesis and activity between heterocysts and vegetative cells growing in N2, ammonium and nitrate grown conditions. A procedure was developed for the isolation of heterocysts from cyanobacterial filaments and a polyclonal anti- ...

Hsue, Kuo-Chiang, 徐國強

碩士 / 國立中興大學 / 食品科學系 / 86 / AbstractMuscle of tilapia（Orechromis niloticus）was stored under high hydrostatic pressure from 500 to 3,000 atm for 12 hr at 25℃ to investigate the changes of freshness and the denaturation of muscle proteins. The tilapia muscle after pressurizing was also stored at normal condition（25℃, 1 atm） to determine the changes of K values and microorganism numbers in order to investigate...

Lin Chia-Hao, 林家豪

碩士 / 國立清華大學 / 生命科學系 / 90 /

King, Huei-Zeon, 金蕙蓉

碩士 / 國立臺灣體育學院 / 體育研究所 / 90 / King, Huei-Zeon (2002).The title of thesis: The Study on the Obstacle Factors for Leisure Activities of Countryside Women -in YuCht, Nanto-Unpublished master’s thesis. National Taiwan College of Physical Education, Taichung. Total page number; 176 pages. ABSTRACT This research is a study on the samples from a specific region and group of people. The main purpose of it was to unveil the factors that keep the women in the countryside ...

Feng-Cheng Wu, 吳豐成

博士 / 國立中山大學 / 海洋生物研究所 / 90 /

Wei-I Lu, 呂威頤

碩士 / 國立中山大學 / 海洋生物研究所 / 90 / The family Pegasidae is found composed of 5 species in two genera, Eurypegasus Bleeker and Pegasus Linnaeus. They contain two (E. draconis (Linnaeus) and E. papilio (Gilbert)) and. three species (P. laternarius Cuvier, P. volitans Linnaeus and P. Lancifer Kaup) , respectively these species are all small, benthic, and covered with plastron from head to tail. Three species have been recorded in Taiwan, but there are many differences between the species names and the sp...

吳筱萍

碩士 / 國立高雄師範大學 / 生物科學研究所 / 90 / The objectives of this study were to evaluate the population expanding potential of an exotic fish species, many-rayed sailfin sucker catfish (Pterygoplichthys multiradiatus), and its impact on endemic lotic organisms in Taiwan. I investigate the population characteristics, reproductive cycle, and feeding content from December 1999 to November 2000. During the study period, 537 individuals were collected. The standard length of collected individuals ra...

Mu-Shiuan Shie, 謝沐璇

碩士 / 國立東華大學 / 自然資源管理研究所 / 90 / This study aims at realization on annual and seasonal variation of scombrid assemblage in the edge of Kuroshio, and exploration on the driving force of assemblage variation as well. The composition of scombrid assemblage is estimated from daily catches of set net from 1993 to 2000. The historical data of environmental factors is supplied form weather stations. Kuroshio current provides a transport mechanism to pelagic scombrid migrating to Taiwanese coast. The ...

Po-Hsin Su, 蘇柏欣

碩士 / 國立中興大學 / 獸醫學系 / 93 / The aims of this study are to develop an accurate method for determination of tissue residual levels of cobia fed with amoxicillin (AMX). AMX was extracted from various cobia tissues in phosphate buffer solution by solid-phase extraction. Then samples were subjected to derivatization by trichloroacetic acid (TCA) and formaldehyde in boiling water for 30 mins. HPLC separation of AMX was carried out on a XTerra® C18 column with mobile phase of 25 mM K2HPO4 (pH 8.5) and ac...

Wan-Chun Huang, 黃婉君

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 93 / The conditions of pretreatments with alkali reagent were conducted to investigate its effect on the quality improvement of gelatin extracted from tilapia skin using acetic acid or phosphoric acid. Acetic acid extraction of gelatin was taken after tilapia skin was soaked in 0.1 NaOH solution for 1 h and sufficiently washed, then divided to three groups and extracted at 50℃ for 1 h, 60℃ for 30 min and 70℃ for 25 min respectively. The effect of temperature on the ...

Tuan-cheng Lin, 林端正

碩士 / 逢甲大學 / 土木及水利工程所 / 95 / In Taiwan almost all of the newly constructed hydraulic structures have subsidiary fishway design at present. Most of them belong to the stepped pool-weir type fishways. The fishways are not very effective since the characteristics of the river geology and the fishes’ attributions are not considered in the designs. This research evaluates the feasibility of the full cross-section fishways in order to supply more useful passageways for the fishes to migrate upstream. T...

Wei-Liang Yan, 顏維良

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Tilapia were fed with feeds of isocalorie (3972 ± 38 kcal/kg) and equal protein content (48.29 ± 1.03 %) for 8 weeks. The plasma total cholesterol concentrations of groups fed freshwater clam extract (FC) and Japanese clam extract (JC) were 272.3 ± 64.2 and 271.8 ± 86.2 mg/dL, respectively. LDL total antioxidant capacity (TAC) of FC and JC as 0.279 ± 0.004 and 0.255 ± 0.004 mM Trolox equivalent, respectively. LDL α-tocopherol contents in FC and JC were 11.59 ± 1.17 a...

Kuo-Jung Chu, 朱國榮

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 95 / ARNT is one of the bHLH/PAS（Per-Arnt-Sim） proteins that forms heterodimers with HIF, SIM and AhR to regulate different gene expression, such as hypoxia inducible-related genes, xenobiotic metabolic and neuron development. Previous investigation found that knockdown of arnt2a/b/c prevented embryo retina neuron and lens cell differentiation and repressed neuroD transcription. During early stages of development, members of hif-α are constitutively expressed in the hin...

Yi-Chih Chen, 陳宜芝

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 95 / Abstract The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor that belongs to the basic helix-loop-helix PAS (bHLH-PAS) protein family. AHR mediates the biological and toxic effects of various environmental pollutants such as dioxin or polycyclic aromatic compounds. The AHR/ARNT complex also regulates numerous genes involved in a variety of physiological process. In mammalian system, a negative regulatory loop of AHR-related signaling path...

Li-Che Chen, 陳立哲

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 95 / Abstract The p53 is a tumour suppressor protein.The ability of p53 to suppress tumorigenesis is mediated through its response to cellular stress, which culminates in cell growth arrest, apoptosis or senescence.The mdm2 (murine double minute 2 ) gene is an oncogene. In unstressed cells, p53 is maintained at low levels by the action of MDM2, an oncogenic E3 ubiquitin ligase (ubiquitin-protein ligating enzyme).The E3 ubiquitin ligase activity of MDM2 is essential to m...

Jian Ya-Huei, 簡雅蕙

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / Abstract Grey mullet, Mugil cephalus is an important commercial species cultured in Taiwan. Due to their valuable ovary product cultured grey mullet can make more profits than other fish species. There are five grey mullet strains found in Taiwan. The superior quality of the ovary product was obtained in some strains and the poor ovary maturation will impede in this industry. However, it has been difficult to distinguish from their ovary size and gonadosomatic inde...

Sheng-Ming Wang, 王聖銘

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 /

Zhi-Gu Huang, 黃至谷

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 /

Wang Yu-Chen, 王玉珍

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / Abstract 　　Streptococcus iniae causes a fatal meningoencephalitis and is associated with high mortality in farmed fishes. It may appear chronic or unobvious infection. Characteristic symptoms include exophthalmia (which may be bilateral), corneal opacity, melanosis, lethargy, loss of orientation, swimming erratically, dorsal rigidity, vertebral deformity, tachypnoea, anorexia, emaciation, or petechiae around the anal zone. This study amplified the pgm gene of Strep...

彭偉莉

碩士 / 國立臺灣大學 / 生化科學研究所 / 73 /

江盛宗

2010年9月「中」日釣魚台海域撞船扣人事件，最終在中共使出撒手鐧：「禁止對日輸出稀土」，始獲日本願意放人而平和落幕。然而中共這式絕招，卻坐實了美國等西方國家的深刻隱憂，即擔心中共食髓知味，日後頻以稀土資源作為談判施壓的手段。源自1990年起，中國大陸開始大幅開採境內稀土礦賣資源，使之逐步成為世界上主要的稀土礦物出口供給國家，而基於政治上的對立以及對本國高附加價值工業的保護，美國等34個國家特別針對中共、伊朗及北韓等國訂定「瓦聖那協定」英文：The Wassenaar Arrangement），限制中共在稀土礦產提煉技術的取得、研發與發展，將中共的提煉技術約束在「低階技術」層面。同時，美、日等國為因應中共大量開採稀土資源而建立了稀土戰略儲備機制；此兩項國際間針對中共而訂立的限制，使得中共啟發要建立如OPEC(Organization of the Petroleum Exporting Countries，石油輸出國組織)國家一般，對於本國天然資源有壟斷、獨佔、定價權以及取得國際事務上更強而有力的影響力與發言權的構思。中共利用控制稀土出口配額限制的策略，更在2010年9月中日釣魚台事件...

Chu, Wai-Kwan, 朱慧君

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 107 / Dead end (dnd1) gene encoding an RNA binding protein with conserved RNA recognition motif (RRM) is specially expressed in primordial germ cells (PGCs) and germ cells of vertebrates. The DND1 is essential for PGCs migration, survival and may also play an important role in maintaining PGCs cell fate. In this study, we report targeted mutagenesis of dnd1 gene in the zebrafish model and ornamental freshwater angelfish (Pterophyllum scalare) by genome editing to obtain ...

Yang, Chia-Hsun, 楊佳勳

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 107

Chen, Sih-Hua, 陳ㄙ華

碩士 / 國立臺灣海洋大學 / 海洋事務與資源管理研究所 / 104 / The possible stock structure of bony flyingfish (Hirundichthys oxycephalus) and yellowing flyingfish (Cypselurus poecilopterus) in surrounding waters of Taiwan were examined based on both otolith morphometry (including morphometric indices and elliptical Fourier analysis) and microchemistry of core of the otolith (including Sr/Ca, Ba/Ca, Mg/Ca, Zn/Ca and Mn/Ca ratios) in company with other previous studies. Fish samples were collected from northern, southern ...

Min-Ting,Liao, 廖敏廷

碩士 / 中國文化大學 / 化學系應用化學碩士班 / 107 / Part1. Genetic pathway of maternal transmission in Topiramate-treated zebrafish model Zebrafish (Danio rerio) is one of model organism for developmental biology in recent years. As an experimental animal, zebrafish has many advantages. For example, its embryos are easy to collect and reproduce. Zebrafish grows rapidly and has similar heredity to humam beings. Therefore, it is widely used in animal genetics, embryonic development, drug screening, toxic drug t...

Liou, Zih-Ling, 劉子綾

碩士 / 國立臺中教育大學 / 科學教育與應用學系碩士班 / 106 / To reduce the side effect of chemical compounds in human body, traditional Chinese herbs for whitening is popular in nowadays. Persicae semen、Armeniacae amarum semen and Coicis semen are in this category. The purpose of this study was compared the effects of three traditional Chinese herbs－Persicae semen、Armeniacae amarum semen and Coicis semen on the development of zebrafish melanin. We used zebrafish (pronounced star-AB) from Taiwan Zebrafish Core Facility ...

Yu-CheHsieh, 謝侑哲

碩士 / 國立成功大學 / 機械工程學系 / 106

Chang, Hung-Ju, 張弘儒

碩士 / 南臺科技大學 / 生物科技系 / 106

Luo Jie, 羅捷

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 105 / Duckweed is an aquatic plant that can play the best role in an aquaponics system for its being very productive and capable of cleaning the water in which the aquatic animal inhabits. Here we used Lemna minor, one of the most studied duckweed species. In Experiment 1 (Chapter 2), we successfully employed image analysis system to get covering area of duckweed and estimate duckweed fresh dry weight through weight-area correlation analysis. Considering the convenience ...

Chen, Sai-Wei, 陳思瑋

碩士 / 國立屏東科技大學 / 生物科技系所 / 105

Wan-LunTaung, 唐婉倫

碩士 / 國立成功大學 / 生物科技研究所 / 105

Hsin-Chuan Hsiao, 蕭新泉

碩士 / 國立海洋大學 / 水產養殖學系 / 88 / Two separate experiments were conducted to determine the levels of dietary cod liver oil needed for maximum weight gain of grouper in experiment Ⅰ（Ⅰ-1 andⅠ-2）. Seven isontrogenous and isoenergetic diets with levels ranging from 0 to 12% in 2% increments and 0 to 10% in 2% increments and a control group （fat-free ingredients）were evaluated in each experiment. After termination of both experiments, groupers fed treatment diets had insignificant difference in weight gain...

Ting-Chang Chang, 張庭彰

碩士 / 國立臺灣大學 / 漁業科學研究所 / 102 / There have been many studies to enhanced the percent weight gain and feed efficiency by added digestive enzymes in the commercial diets of stock raising,but less in aquaculture.The aim of present study was to assess the best amount according to the weight gain、feed efficiency and protein efficiency ratio(WG、FE、PER) and observation the intestine morphology of adding natural digestive enzymes in the diets of orange-spotted grouper(Epinephelus coioides) for five weeks....

Chen, Chien-Hung, 陳建宏

博士 / 國立臺灣海洋大學 / 食品科學系 / 102 / Food safty is an issue that becomes more important around the global. Recently, for different reasons, including religion, race, allergies, the abuse of endangered species and the heedless use of cheap or toxic fish, previously developed techniques in species identification have been improved in manufactured products. DNA-based methods offer an alternative approach to species identification by using polymerase chain reaction (PCR). In the present studies, mitochondr...

WU, LONG-JING, 吳龍靜

碩士 / 國立海洋大學 / 漁業研究所 / 77 /

Chin-Yan Tsai, 蔡青彥

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 97 / bHLH-PAS is a protein family that play important functions in vertebrate development, cellular homeostasis, and metabolic balance. It includes the aryl hydrocarbon receptor nuclear translocator (ARNT) and its associate factor, hypoxia-inducible factor alpha subunit (HIF-α). The unstable HIF-α subunit act as intracellular oxygen sensor and it is stabilized by oxygen deficiency. Under hypoxia stress treatment, HIF-αsubunit associates with ARNT to form a heterodimeric...

Tzu-Husan Yu, 游子萱

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 97 / Hypoxia-inducible factors (HIFs) are belong to bHLH-PAS protein family. HIFs are composed of two subunit, HIF-1a and HIF-1β (aryl hydrocarbon receptor nuclear translocator, ARNT). During hypoxia, HIF-1a stabilizes oxygen homeostasis by associating with ARNT to form a heterodimer complex and specifically binds to a 5’-RCGTG-3’ hypoxia-responsive element (HRE) in the promoter or enhancer of various hypoxia inducible genes to stimulate transcription of target genes. 　...