Yin-Shan Chang, 張銀珊

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 99 / Zebrafish(Danio rerio) has been extensively used as a model organism for vertebrates and the alkylating agent ENU (N-ethyl-N-nitrosourea) is commonly used to generate a large scale of mutation because of its ability to cause DNA damages in spermatogonial stem cells. In this study, an ENU-generated zebrafish mutant(snake) was taken to examine whether changes in morphological features and defects in the gastrointestinal system had occurred. The eyes and heads of snak...

Yi-Chun Hsieh, 謝宜君

碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / Common carp (Cyprinus carpio) is a freshwater fish with high tolerance to low water temperature and low dissolved oxygen, and its optimal water temperature is around 20-27oC. They are still alive when water temperature decreases to 4oC. In addition, common carp in anoxia can also tolerate dissolved oxygen down to 1 ppm. Head kidney is the most important lymphohematopoietic organ in teleost fish. It was found that zinc contents in the head kidney of common carp are te...

Chang-Han Lee, 李昶漢

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / The objective of this study was to investigate the pantothenic acid metabolism in hybrid tilapia (Oreochromis niloticus × O. aureus) with a modified cannulated urine collector through the observations on the 24-hour changes of plasma and urine concentration of pantothenic acid and Coenzyme A at various sampling time points as well as the 24-hour organ accumulation of these two metabolites after the initiation of exogenous pantothenic acid input either by oral admin...

Hsi-Pi Huang, 黃熙弼

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / The objective of this study is to investigate the effect of dietary protein and lipid on the growth performance and body composition of juvenile rock porgy (Oplegnathus fasciatus). In experiment 1, by using white fish meal as protein source, cod liver oil as lipid source, α-cellulose and α-starch as energy regulators, duplicate groups of juvenile rock porgy were fed with five groups of isocaloric diets containing the same dietary lipid level (8 %) but different diet...

Ming-Jeng Chen, 陳明政

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 99 / Apoptosis, the programed cell death, plays an critical role in cancer therapy currently. BikDD, a T33D/ S35D mutant of pro-apoptotic protein Bik, has been used to eliminate cancer cells in pancreatic and lung cancers. Therefore, we hope to expand the application of BikDD to not only in cancer therapy but also in the researches of embryonic development and regenerative medicine. Fisrt, we designed a temporally and spatially controlled tissue-specific cell ablation...

Yun-Ting Juan, 阮沄庭

碩士 / 國立臺灣大學 / 漁業科學研究所 / 99 / The purpose of this study to know the effect of Schizophyllan is extracted from the culture medium of Schizophyllum Commune on non-specific immune response of Tilapia by oral administration. The preparation different concentrations of polysaccharide solution and with give different number of observations of different Tilapia non-specific immune effects. Daily observe of non-specific detection of immune factors, including the superoxide anion production, nitric oxide ...

Shih-Wei Tan, 覃世偉

碩士 / 國立臺灣大學 / 農業化學研究所 / 99 / Nanoscale zerovalent iron (nZVI) has been increasingly used for in situ groundwater and soil remediation because of its high redox activity and specific characteristics of nanoparticles (NPs). Recent applications are often involved in the use of surface-modified nZVI with the stabilizer that can enhance mobility of nZVI while travelling through the underground aquifer. However, the ultimate fate of iron NPs under- or above-ground aquifer and associated risks of expos...

Wei-Ren Chen, 陳暐仁

碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / The fisheries resources depleted in recent decades, fish stocking were widely adopted to enhance the resources in many countries. The release effects were usually poor due to its assessments not enough, wrong operation or low survival and foraging ability of fishes, especially for those group-living animals which often got social information to elevate individual fitness. The shoaling behavior is important for information transfer, anti-predator responses and foragi...

Huei-Jyun Hu, 胡惠鈞

碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / Fish face harsh and fluctuating ion composition in fresh water, therefore it is important that fish have to develop the regulatory mechanisms for ion homeostasis. Vitamin D3 is a group of fat-soluble secosteroids. It functions as a hormone and participates in many physiological reactions in animals. Vitamin D3 has been documented to be an endocrine of regulating ion uptake in mammals, however, the role of vitamin D3 on the different ions is still unclear in teleosts...

Li-Hsin Huang, 黃莉欣

碩士 / 國立臺灣大學 / 生化科技學系 / 101 / Mushroom molecular pharming, an emerging biotechnology application using mushrooms as bioreactors to produce pharmaceutical proteins or industrial enzymes, exhibits advantages of being safe, easy in manipulation, and less expense. Agrobacterium-mediated transformation has been established and the heterologous gene expression has also been enhanced by various molecular biology strategies in our laboratory. The previous study shows that the major green fluorescence was...

Yi-Sen Wang, 王奕森

碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / Grouper (Epinephelus spp.) are highly economic value farmed fish in Taiwan. In addition to the farming competition from China and Southeast Asia countries, the high mortality caused by iridovirus infection became one of the thorniest issues in grouper aquaculture. Therefore, the study of mechanism involving in the iridovirus infection is the most important current events. Using cDNA microarray assay, our laboratory had identified 21 immediate early genes from groupe...

Zi-Hua Chen, 陳姿樺

碩士 / 義守大學 / 生物技術與化學工程研究所碩士班 / 99 / Anisomeles indica (L.) O. Ktze grows mainly in subtropical regions. In Taiwan,it frequently appears in forest edge or abandoned ground of the low-elevationmountains. People often used it as folk medicine to treat rheumatoid arthritis,gastroenteritis, and other inflammatory diseases. However, very few studies reported on the inhibitory effects of A. indica on the proliferation, metastasis, migration and invasion of oral cancer cells. The statistic data from Depa...

Lu, Wen-Chen, 盧玟丞

碩士 / 國立高雄餐旅大學 / 餐旅管理研究所在職專班 / 99 / ABSTRACT The purpose of the study is to explore the development of black tea industry in Taiwan. It takes the most centralized area of Taiwan black tea-Yuchih Township as the study subject to research the industry development and future vision. The reasons why we take black tea producers in Yuchih Township as the study subject are as follows: the area is the most centralized and most famous place for planting black tea. Especially after the 921 earthquake,the g...

Ching-I Li, 李靜宜

碩士 / 輔英科技大學 / 護理系碩士班 / 99 / The interaction between human and pet animals could improve effectively social interaction; reduce pain, loneliness and depression of cancer patient, psychological patient, long term institutional habitant and pediatric patient. The pet therapy usually took dogs or cats as subject, pet fish was seldom chosen. However, the former cost and spend more time than latter because of staffing, training, health keeping and infection controlling. Most of studies of pet therapy ...

Chin - Wei Chang, 張晉維

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 100 / Autocrine motility factor receptor (AMFR) is a glycoprotein originally named gp78. AMFR/gp78 plays a dual role as a cytokine receptor on the cell surface or as an E3 ubiqui¬tin ligase involved in endoplasmic reticulum associated degradation (ERAD). AMFR has important clinical implication, which has prognostic value in clinical cancer research. However, the exact function of AMFR in embryo¬nic development remains un¬clear. Using in situ hybridization to detect ...

Chen, Mei-Hua, 陳梅華

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 99 / Tilapia skin protein (TSP) was hydrolyzed by using proteinases with various hydrolysis times (1-11h). Radical scavenging activities (including 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and superoxide anion radicals) of the TSP hydrolysates (TSPHs) were evaluated. In addition, angiotensin Ι converting enzyme (ACE) inhibitory activity and the protective effects on H2O2-induced DNA oxidative damage (DNA protective effect) of the TSPHs were investigated. The...

Huang, Hueiya, 黃慧雅

碩士 / 國立高雄海洋科技大學 / 海洋環境工程研究所 / 99 / Three sampling points (upstream Bakong Bridge, midstream Dehuei Bridge and downstream Xingzhong Bridge) are set at Hou-Jing River area from March to November 2010, in order to perform the sampling of water, sediment and Tilapia. Inductively coupled plasma atomic emission spectroscopy (ICP-OES) is used to perform heavy metal concentration analysis of Environmental Protection Bureau standard, including arsenic, lead, cadmium, chromium, copper, zinc and nickel, an...

Zheng-you Chen, 陳政佑

碩士 / 慈濟大學 / 分子生物暨人類遺傳學系碩士班 / 99 / Liver regeneration is a complex process which involves regulations of various signaling pathways. Inhibition of fibroblast growth factor (Fgf) signaling specifically in hepatocytes results in delayed liver regeneration after partial hepatectomy (PH) in transgenic mouse and zebrafish. therefore, Fgf signaling is crucial for 　 regeneration process. However, the detailed mechanisms are still unclear. In this study, we first established different PH models with vari...

Hsin-pei Hsieh, 謝欣蓓

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 99 / Nervous Necrosis Virus (NNV) is the viral nervous necrosis disease pathogen which infection grouper larvae, cause high mortality, and belonging to the family Nodaviridae, genus Betanodavirus. The aim of this study was to develop a high-sensitivity, specificity and quantifying of NNV TaqMan qRT-PCR assay. In addition, we used an internal RNA standard for the detection to increase accuracy. NNV TaqMan qRT-PCR assay will be useful for the development of aquacultur...

Jia-Jhou Lee, 李鎵州

碩士 / 國立高雄海洋科技大學 / 漁業生產與管理研究所 / 98 / This study is aimed to investigate the reproductive biology of big head pennah croaker, Pennahia macrocephalus, in the coastal waters off southwestern Taiwan. A total of 1,110 specimens were collected monthly from the catches of trawl between September 2008 to September 2009, from the landings on Ketzliau fish market of Zihguan Township, Kaohsiung County. By means of histological examination and the oocyte development can be clearly categorized into nine stage...

Wu, Meichih, 吳美枝

碩士 / 國立聯合大學 / 客家語言與傳播研究所 / 99 / This research focuses on the area of Li Yu Tan Village in San Yi Town. Due to its unique geographic location, this area appears to have more diversified ethnic groups compared with other areas in the San Yi Town, notwithstanding the majority Hakka population. The prevalence of Hakka language has gradually declined as the Hakka people started to come into contact with other ethnic groups in San Yi Town, leading towards bilingualism. This phenomenon is particularly ...

Chung Yun-Lung, 鐘云隆

碩士 / 亞洲大學 / 保健營養生技學系碩士班 / 98 / Cytochrome b gene (Cyt b) is a functional gene between tRNAGlu and tRNAThr in mitochondria DNA (mtDNA), and which was usually used to study about phylogeny and evolution in vertebrate. In 2003, the so-called “DNA barcode” was proposed and allocated to identify organisms based on the sequences of cytochrome c oxidase subunit I (COI) gene in the mitochondria. In order to identify the original species of raw materials and processed products, the complete Cyt b (1137 b...

Ming-Yen Tzeng, 曾明彥

碩士 / 國立臺灣大學 / 漁業科學研究所 / 98 / To understand the life history traits and the migratory behavior of grey mullets in the Tansui River System, 826 grey mullets were collected during different seasons from 34 sampling sites located on the river mouth of Tansui River to the upper streams, Xindian and Keelung Streams, from April, 2008 to December, 2009. At the same time, the environmental factors such as salinity and algal and benthos densities were also measured. The morphological traits and the monthl...

Yi-Zhen Lin, 林依臻

碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 98 / Glycogen synthase kinase3β (GSK3β) has multiple biological functions. However, the role of GSK3β in angiogenesis during embryonic development is unclear. To address this issue, we firstly analyzed the expression patterns of GSK3β during early embryogenesis. Immunostaining revealed that GSK3β was distributed in somites, especially at the somite boundary. Cell transplantation studies showed that GSK3β modulated angiogenesis in a non cell-autonomous manner. Next, we...

Hao-Hsuan Hsu, 徐浩軒

碩士 / 國立臺灣大學 / 漁業科學研究所 / 98 / Ionocytes, mitochondrion-rich cells (MRCs), are specialized epidermal cells which maintain ionic homeostasis of body fluid by actively taking up or excreting ions. The purpose of this study is to examine the regulatory mechanism of cell turnover and transporters expression of ionocytes in Japanese Medaka (Oryzias latipes) during seawater (SW) acclimation. Four types of ionocyte were identified in medaka embryo, the freshwater (FW) type were NHE cell, NCC cell and HA ...

Pei-lin Chao, 趙珮伶

碩士 / 國立臺灣師範大學 / 生命科學研究所 / 99 / Aquaporins (AQPs, water channels) are integral membrane proteins that facilitate water transport across cell membrane. However, some member of AQPs was also found to facilitate transport of carbon dioxide (CO2), glycerol, ammonia, and urea. According to their properties, AQPs were divided into three functional groups: aquaporins, aquaammoniaporins, and aquaglyceroporins. In mammalian studies, AQP1-deficient erythrocytes were found to decrease permeability to CO2. I...

Jun-wei Ye, 葉峻瑋

碩士 / 大同大學 / 生物工程學系(所) / 99 / D-Amino acid oxidase (DAO) is a flavoenzyme ubiquitously present in eukaryotes. It can catalyze the oxidative deamination of D-amino acids to produce the corresponding keto acids, NH3 and H2O2. Besides being well-known as industrial biocatalyst, this enzyme has been proposed to have novel physiological functions in animals. In this study, we traneformed and overexpressed DAO cDNA genes from zebrafish、Haematonectria haematococca 9404010、94071401and 96030313 (Hh01, 02 ...

You-nan Lin, 林祐男

碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 99 / In this study, a LC/MS/MS method for the determination of ampicillin antibiotic in fish muscle tissue was developed and accredited according to Commission Decision 2002/657/EC. The metabolism of ampicillin in tilapia was them studied in serum, liver and muscle. The homogenized fish tissue was first extracted with MeOH-H2O(4:1), C18 sorbent was added to remove lipids and impurities, the extract was then evaporated to dryness with a steam of nitrogen gas at ...

Yu-Guang Chen, 陳昱光

碩士 / 國立中山大學 / 經濟學研究所 / 99 / The grey mullet have been regarded as the treasure, so it is the very precious fishery resource in Taiwan. However, from the historic catches, it showed the amount of the grey mullet is decreasing every year and the reasons are not only influenced by the climate change, but also the catches from the vessels of PRC. The main purpose of the study is the effects of the implementation on individual transferable quota of the cross-strait in grey mullet to the living of fis...

Meng-chung Wu, 吳孟珺

碩士 / 國立中山大學 / 海洋生物研究所 / 99 / Persistent organic pollutants (POPs) have attracted the attention of scientists because they have the following characteristics: long-distance transmission, persistence, bioaccumulation and chronic toxicity. Due to their high hydrophobicity, POPs are easily absorbed by the suspended particles in the marine environment. The suspended particles then settle into the soil may pass through the food chain, resulting in bioaccumulation and biomagnifications and causing pote...

Hsiao,Chin Shin, 蕭志欣

碩士 / 國立勤益科技大學 / 冷凍空調系 / 98 / This study is aimed at exploring the feasibility of applying renewable energy to post-modern agriculture. This research utilized solar PV system and DC driver high-efficiency water pumps to set up hydroponics and aquaponics, which is a symbiotic cultivation of plants and aquatic animals in a reciprocity environment. This study shows that the electrical equipment and the environment control technology adopted in ...

Hung,CHhiaoling, 洪巧齡

碩士 / 明道大學 / 設計學院碩士班 / 101 / This research sees pearls as a visual symbol. For they are round and bright, as a metaphor or a symbol, pearls can carry feminine and tender messages. To clarify what “Pearl” and “Tears of the Mermaids” in common both as a cultural symbol, this research use Andersen’s fairy tale “The Little Mermaid” as the original text to find the story elements related to “Pearl”. As a result, this research finds out the important symbols of this story: “Ocean”, “Feminine” and “Tole...

Shean, Bean-Jean, 沈必正

碩士 / 國立海洋大學 / 水產養殖學系 / 84 / Abstract The experiment was to simulate the effects of acid rain on the accumulation of heavy metals in muscle of tilapia (Oreochromis niloticus, L). Tilapia averaged 10.5 g were reared for 8 weeks in water containing 4 metals with 3 concentrations (Cu: 3.5, 7.0 and 14.0 ppm; Cd: 0.4, 0.8 and 1.6 ppm; Pb: 7.0, 14.0 and 28.0 ppm; Zn: 13.0, 26.0 and 52.0 ...

XIA, HUI-LIAN, 夏蕙蘭

碩士 / 輔仁大學 / 生物學研究所 / 74 /

Lin, Zheng-Yi, 林正義

碩士 / 國立臺灣大學 / 海洋研究所 / 69 /

Lin, Shih-Hsiu, 林式修

碩士 / 國立海洋大學 / 水產製造學系 / 69 / Eel, culture-breeding fish, is one of most important export fishes in Taiwan. Usually, content of amino acid component can be used as an index of eel flesh quality. For rapidly, sensitively and accurately measuring amino acid component, this study used gas chromatography to survey amino acid content. In this study, we wanted to find out the best conditions for measuring the amino acid content of eel flesh and processing products. The results are as follow: 1. ...

Lin, Chien-Chun, 林建君

碩士 / 國立中興大學 / 食品科學系 / 85 / The cultured milkfish were used as raw materials for washing in difference ion strength. Membrane technology was applied to recover the proteins from washed solutions and to improve pollutionproblems. Gel properties of milkfish meat added with protein powderprepared by membrane were determined. The effect of washing conditions containing washing cycles, time, and water : flesh rat...

Guo, Jia-Chun, 郭嘉淳

碩士 / 國立中興大學 / 食品科學系 / 85 / The gel strength, whiteness, and water holding capacity of theminced milkfish gel induced by heating, heating after pressurizing,and two-step heating heating were compared. Enzymatic activity of temperature, pH, hydrostatic pressurization, and heat treatment on the protease activity were also investigated. The results obtainedwere as follows:1.The suwari of unwashed milkfish meat ...

Yu, Hao-Cheng, 余浩成

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 93 / Tilapia is the most general aguacultivating fish in the fresh water and has characteristics of fast growing and well disease resistance. Thus tilapia is an important animal model for relative aquaculture researches. Hepatocyte nuclear factors (HNFs) are liver-enriched transcription factors, which can activate the expressions of tissue-specific, growth and development related genes. The expression of HNFs was detected in the gonads of tilapia previously, and which...

Chih-Chien, Hsu, 許志堅

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 94 / Hepatocyte nuclear factor (HNF)-4 is a liver-enriched transcription factor and belongs to the highly conserved member of the nuclear receptor superfamily. HNF-4 together with other factors play a key role in the tissue specific expression of a large number of genes involved in lipid and glucose metabolism. However, its function in fish is still poorly understood. In our previous study, RNAs and proteins of HNF-1, HNF-1, and HNF-3are detected in the liver, ova...

Huang Li Fang, 黃麗芳

碩士 / 國防管理學院 / 國防資訊研究所 / 94 / With the growing development of information technology and Electronic Commerce (EC), the criminal behavior emerges in an endless stream. For the user who is fond of internet activity, it brings serious harm. Phishing is one of the internet criminal behavior. Though the fabricated email subject and content to steal information and do illegal act. These behavior are seriously become threat to the information safety. Army is also able to develop Military-Net. It not onl...

Hsu Yung-Nien, 徐永年

碩士 / 大葉大學 / 分子生物科技學系碩士班 / 94 / Biosafety has been a new concept while the biotechnology making continuous progress and lots of transgenic and cloned animals are produced. Sterility is a necessary adjunct to the exploitation of transgenic animals unless completely secure and locked facility is available. The development, growth, reproduction and physiological metabolism of animals are tightly connected with endocrine system. The hypothalamus-pituitary-gonads axis and the expression of related gen...

jung-chang chu, 朱榮昌

碩士 / 國立海洋大學 / 食品科學系碩士在職專班 / 90 / ABSTRACT The solubility of mackerel viscera was 65.5 % after being hydrolyzed at 50℃ for 5hrs. The pH of the hydrolysate decreased with the progress of the reaction, while the amounts of total nitrogen (TN), non-protein nitrogen (NPN), free amino acid (FAA) and ammonia increased. The major FAA in the mackerel viscera hydrolysate were taurine (Tau), aspartic acid (Asp), alanine (Ala), glutamic acid (Glu), leucine (Leu), and lysine (Lys). As compared to ...

Fu-lung Yeh, 葉富隆

博士 / 國立海洋大學 / 水產生物技術研究所 / 90 / Heat shock proteins (HSPs) with different molecular weights are rapidly synthesized within stressed cells after exposure to environmental stresssors. Among the stress-inducible HSPs, the members of HSP70 function as a molecular chaperone. Besides the stress-inducilbe HSP70, a form of HSP70 called heat shock cognate protein 70 (HSC70), which is constitutively expressed in unstressed cells under physiological conditions. A spontaneous high expression of heat shock pr...

林順啟

碩士 / 國立海洋大學 / 水產生物技術研究所 / 90 / Abstract Zebrafish hoxa1a is a homeobox gene. It encodes a polypeptide including a 60 amino acid homeodomain. Seven diagnostic residues that confering specific DNA binding locate in the homedomain. In addition, there are conserved hexapeptide sequence, WMKVR, that lies 19 or 20 amino acids N-terminal of the homeodoamin. Zebrafish hoxa1a has a splice variant resulting two different length mRNA. Results of whole-mount in-situ hybridization showed that hoxa...

黃宗賢

碩士 / 國立海洋大學 / 水產生物技術研究所 / 90 / Abstract A full-length cDNA with 1100 bp encoding a putative Manganese-superoxide dismutase ( Mn-SOD ) from diatoms ( Thallassiosira weissflogii ) was cloned. Nucleotide sequence analysis indicated that it comprises a complete open reading frame coding for 218 amino acid residues. The deduced amino acid sequence showed high similarity (49 ~ 65%) with that of Mn-SOD from other species. Computer analysis of the residues required for coordinating the single...

C.Y. Shih, 施錦毅

碩士 / 國立海洋大學 / 水產生物技術研究所 / 90 / The expression of distorted DNA-binding factors was studied in developing zebrafish using UV-damaged DNA as the binding target. A strong and high-shifting binding activity was detected in the extracts of zebrafish embryo (12 h after fertilization), and the expression of the activity dramatically decreased in 60 to 84-h-old zebrafish. Unlike the embryonic extract, the extracts of 84-h-old larvae produced only low-shifting binding complexes when incubated with a CPD-...

Jia-Pey Wang, 王嘉佩

碩士 / 國立海洋大學 / 水產生物技術研究所 / 90 / Proteins of the Bcl2 family play important roles in animal development; however, the underlying molecular mechanisms of their actions are poorly understood. As part of our undergoing effort to develop zebrafish embryos as a model system for the embryonic study of the Bcl2 family proteins, here we described the cloning and characterization of the third members of the zebrafish Bcl2 protein family, zfBax1. The zfBax1 cDNA is 1938 nucleotides long, encoding a polype...

Yi-Hui Hwang, 黃議輝

碩士 / 國立海洋大學 / 食品科學系 / 90 / Abstract The mucus which being secreted by the mucus cells present in the fish epidermal layer was considered to decrease the resistance during fish swimming and protect fish from predator, microorganism and parasite attack. In early study, the composition of fish mucus has reported to contain glycoproteins or proteins which had biological activities. Japanese eel (Anguilla Japonica) is majority of cultured eel in Taiwan. On food processing, eel is one kind ...

姚政源

碩士 / 國立海洋大學 / 食品科學系 / 90 /