Pei-An Liu, 劉培安

碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 / To quantify the dietary manganese (Mn) requirements and its effects on immune responses of grouper, Epinephelus malabaricus, organic Mn (Mn peptide) and inorganic Mn (MnSO4) were each added to the basal diet at 1.75, 9.73, 15.23, 20.23, 23.89, 34.63, 54.54 mg Mn/kg diet, and 2.11, 3.73, 8.15, 12.07, 15.60, 24.92, 39.07 mg Mn/kg diet, respectively. Basal diet without Mn supplementation (containing 1.38 mg Mn/kg diet) was used as control. There were total of 15 exper...

Chong-Jing Huang, 黃重景

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / Color and integrity of tail fin of guppy (Poecilia reticulate) affects not only its market price but also mate selection and breeding within a population. Two experiments were therefore conducted: I. Effects of dietary carotenoids on pigmentation, wound healing and growth of tail fin of male guppy and II. Effects of illumination color and timing on guppy’s mate selection. Experiment I was to find out the effects of 4 diets supplemented with 200 mg/kg carotenoids (as...

Tsung-Lin Liu, 劉宗麟

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / bHLH-PAS is a protein family that play important functions in vertebrate development, cellular homeostasis, and metabolic balance. It includes the aryl hydrocarbon receptor nuclear translocator (ARNT) and its associate factor, hypoxia-inducible factor alpha subunit (HIF-α). The unstable HIF-α subunit act as intracellular oxygen sensor and it is stabilized by oxygen deficiency. Under hypoxia stress treatment, HIF-α subunit associates with ARNT to form a heterodimeri...

沈安鄉

碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 / Abstract Two experiments were conducted to quantify the optimum dietary selenium (Se) requirements of juvenile tilapia (Oreochromis niloticus × O. aureus) from either inorganic or organic source, and their effects on immune responses. Experiment I, sodium selenite and selenomethionine were used as inorganic and organic Se source, respectively, each was added to basal diet at 0, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5 and 1 mg Se/kg diet providing the actual dietary values of 0...

Ts''eng-Mei-Chuan, 曾美娟

碩士 / 國立海洋大學 / 食品科學系 / 88 / Abstract Seasonal variations in the level of extractive nitrogenous components were investigated in the muscle of ayu Plecoglossus altirelis cultured in mountain and plain areas of Ilan County. The ranges of proximate composition in the ayu were as follows: moisture 70.3-74.9%, protein 14.3-17.6%, fat 8.1-9.6%, and ash 1.0-1.5%. The level of protein was higher from June to August than that in other seasons, while the level of moisture was lower at the same t...

Chun-Huo Chiu, 邱春火

碩士 / 國立清華大學 / 生命科學系 / 89 / The stream environmental strategic changing affect strongly to fish on the fish larvae stage because of their weak environmental adaptation. Many references indicate that the survival and growth rate of fish larva may affect the community structure of fish. In the past, most of the researches studied on the relationships of fish and environment emphasized on the juvenile or adult stage of fish, few on fish larvae. The present research based on otolith analys...

Pei-Fen Li, 李佩芬

碩士 / 國立嘉義大學 / 食品科學系研究所 / 101

Pei-Hui Tseng, 曾培惠

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 102 / To elucidate the hygienic quality of commercial narrow-barred spanish mackerel products, 51 samples (including 12 raw fish cube and 39 fried fish cube samples) purchased from the supermarket, traditional market and network, were tested to determine chemical and microbial quality, and biogenic amine contents. The average levels of pH, ash content, moisture, water activity, salt content, total volatile basic nitrogen (TVBN), aerobic plate count (APC) and total c...

Hsi-Yuan Chen, 陳熙元

碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / The purpose of this study was to elucidate the effects of dietary phytic acid on zinc (Zn) utilization in juvenile hybrid tilapia (Oreochromis niloticus × O. aureus). The optimum dietary Zn requirements (NZn) was quantified for the species previously with organic Zn (Zn amino acid complex, Zn-AA) and inorganic Zn (ZnSO4) as 10 mg Zn/kg and 30 mg Zn/kg, respectively. In this study, basal diet with 1.5% phytic acid was supplemented with optimum, 5X optimum and 10X opt...

Ya-Jean Wang, 王雅貞

碩士 / 國立成功大學 / 生物學系碩博士班 / 97 / Orange-spotted grouper (Epinephelus coioides) is a fish species with a high economic importance in the aquaculture industry in Taiwan. The high mortalities observed throughout early development such as viral nervous necrosis (VNN) causes the highest mortalities up to 100% always occur among 1-month-old larvae with total body lengths of 2.0 cm. Teleost is the oldest species has the adaptive immune system. However, there is a risk of inducing immunological tolerance i...

Yu-Ching Su, 蘇于菁

碩士 / 嘉南藥理科技大學 / 環境工程與科學系碩士班 / 93 / In the last decade, much of savant efforts have been on studying endocrine-disrupting chemicals (EDCs), or commonly known as environmental hormones. These chemicals can imitate natural hormones and interfere not only the normal endocrinal, but also immune, reproductive and nerve systems in animals and humans. Some EDCs can be found in different water bodies or even in sewage-treatment works (STWs) effluents. The aims of this study were to investigate the effect...

Fu-Chih Hsu, 許富智

碩士 / 國立臺灣大學 / 動物學研究研究所 / 94 / Nervous necrosis virus (NNV) and grouper iridovirus of Taiwan (TGIV) were two important pathogens of reared groupers. Serious mortality caused by NNV usually occurs among the groupers smaller than 2 inches, and mortality caused by TGIV always occurs among groupers larger than one inch. In nature, NNV and TGIV had been detected within the same host. The aim of this study is to study the interaction between NNV and TGIV during co-infection and dual-infection.The accum...

Chen wen-pin, 陳文彬

碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 97 / Amphiprion ocellaris not only is a most traded species of clownfish, but also a popular pet for marine aquarium hobbyists. The aims of this study are to observe breeding behavior and embryonic development and evaluate the effect of Isochrysis galbana tml – enriched rotifer （Brachionus sp.）and Artemia on growth rate, survival rate and stress resistance of Amphiprion ocellaris larvae. One pair of Amphiprion ocellaris ovulated 33 times a year, average spawning int...

Chiang lee-chun, 江麗君

碩士 / 國立海洋大學 / 食品科學系碩士在職專班 / 91 / Abstract The study was to estimate the optimum dietary zinc requirements of juvenile hybrid tilapia (Oreochromis niloticus ´ O. aureus) and grass prawn (Penaeus monodon) and its effect on immume function. In Experiment I, purified diets with 8 levels (0, 5, 10, 20, 30, 40, 50, 100 mg/kg diet) of supplemental zinc were each fed to triplicate groups of tilapia (mean initial body weight 0.55 ± 0.01 g) for 8 weeks. Results indicated that no significant dif...

Yao Chu, 朱曜

碩士 / 國立臺灣大學 / 農業化學研究所 / 103 / Previous study indicates that nPbO2(s) causes inhibition of acetylcholinesterase (AChE) activity in larvae of medaka fish (Oryzias latipes). The bioavailability of nPbO2(s) is affected by particle aggregation and precipitation in water. However the uptake mechanism of nPbO2(s) in medaka fish and its bioaccessibility, bioaccumulation, and toxicity potency to aquatic organisms remain unclear. The objectives of this study is to understand the uptake mechanisms, bioaccu...

楊大緯

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 103

CHENG, SHI-GUI, 陳石柱

碩士 / 國立中興大學 / 獸醫研究所 / 75 /

Chan Shu-Yun, 詹淑雲

碩士 / 國立海洋大學 / 食品科學系 / 89 / The highest viable cell count of Lactobacillus (Lb.) plantarum CCRC 12250 could be obtained while cultured in MRS broth at 37oC for 8 hr. After 36 hr incubation in the same conditions, the cultured broth present the highest relative viscosity (RV). As compare to the extracting efficiency of EAS from strain CCRC 12250 by different heat treatment, the better extracting treatment was under 80oC for 60 min (0.622 g/L). When strain CCRC 12250 cultured in MRS broth at 20,...

Hsu Sheng Han, 許聖翰

碩士 / 國立海洋大學 / 食品科學系 / 89 / Three tilapia surimi were prepared to prossessing special forms of AM, including free AM (type I), Ca++-bridged AM (type Ⅱ), and TGase-induced crosslinked AM. All of them were used as model surimi samples for cooking gelation mechanism studies. Four analytical techniques were adopted to study stepwise AM sol-gel transition of surimi during dynamic heating to form fish-gels. Among them, TSRM was used to measure the texture phase changes. DSC and a new techniq...

LIANG, HONG-XIANG, 梁宏祥

碩士 / 國立海洋大學 / 水產食品科學研究所 / 81 /

ZHUANG, JIE-CHENG, 莊界成

碩士 / 國立海洋大學 / 水產食品科學研究所 / 81 /

Nai-Hui Wang, 王迺卉

碩士 / 國立臺灣大學 / 環境工程學研究所 / 96 /

Han-Sheng Wang, 王漢昇

碩士 / 國立臺灣大學 / 漁業科學研究所 / 97 / Tilapia is one of the main cultured fish in the world and the aquacultured production in 2007 was about 2,500,000 tons. Therefore, the demand of feed in tilapia aquaculture is enormous. In General, tilapia could use plant protein sources and carbohydrate efficiently. So the lipid sources and its efficiency may cause important effect for cost of feed. In present study, red tilapia diets were supplemented with additional 5% lipid from different proportion (0%, 2%, 3% o...

Wen, Zong-Han, 溫宗翰

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 102 / The mudskippers and eel gobies are living in mangroves, estuaries, lagoon as well as the coastal waters with sandy to muddy substratum. In Taiwan, the species diversity of this group has been revised at least consisting of 9 genera and 10 valid species. These two kinds of gobies are divided into two subfamilies of Oxudercinae and Amblyopinae currently. However, the further to detect and research for phylogenetic relationship of this group and advise these two subf...

YI-Da Wang, 王亦大

碩士 / 國立中興大學 / 生命科學系所 / 97 / This research is about antimicrobial peptides, Epinecidin-1 and Hepcidin 1-5, which can help the immune factors of medaka(Oryzias latipes) and grouper(Epinephelus coioides) resist against Nervous Necrosis Virus (NNV). In this research, we discovered that when Epinecidin-1 or Hepcidin 1-5 interacts with NNV in medaka or grouper, the survival rate of medaka would raise to 72% by injecting the mixure of NNV and Epinecidin-1 1μg/fish into the fish ten minutes later since...

CHANG, CHIN-WEI, 張至維

碩士 / 國立臺灣大學 / 漁業科學研究所 / 85 / Grey mullets Mugil cephalus are not only important coastal or inshore species but also very desirable aquaculture species in Taiwan. Adults migrate to the southwestern coasts of Taiwan for spawning during late November through the following late January. However juveniles are captured from early November in the coastal estuaries. These phenomena indicate the disagreement in t...

Lin, Guang, 林廣

碩士 / 國立海洋大學 / 水產生物技術研究所 / 86 /

Ming-Yu Chiu, 邱名玉

碩士 / 中臺科技大學 / 食品科技研究所 / 100 / Lizardfish (Trachinocephalus myops) surimi is commonly used for a additive in surimi-based product processing because of the low price and appreciable whiteness. However, the poor gel-forming ability of lizardfish surimi caused by endogenous proteinase activities might limit their use value. The objective of this study is to determine the biochemical properties of the autolytic proteinases in lizardfish surimi and to investigate the influence of intrinsic and extrin...

Ming-Ann Tsai, 蔡明安

碩士 / 國立屏東科技大學 / 獸醫學系 / 93 / Lactococcus garvieae is a zoonotic pathogen and rare pathogen with a low virulence in human infection. In Taiwan, it causes infection grey mullet and giant freshwater prawn during summer. Gross pathological showed clouding of cornea, splenomegaly and external haemorrhage. Histopathologically, they showed meningoencephalitis. Physiological and biochemical characteristics of all L. garvieae strains were Gram positive cocci. Almost strains were able to grow at high tempe...

Shu-Hua, Hsu, 許書華

碩士 / 國立嘉義大學 / 生物農業科技學系研究所 / 104 / Paclobutrazol (PBZ), a well-known growth retardant of triazole family, is mostly used to control growth and size of crops, vegetables, flowers and turf around the world. Wide and long-term usage of PBZ in agriculture and natural environment has resulted in residual PBZ in food, soil and water; this may lead to high risk of health impairment in organisms. Our previous study indicated PBZ impaired digestive system (including liver, gut and pancreas) development in...

Tsai, Chi-Hang, 蔡季航

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 104

Fang Chun Hsien, 謝豐群

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 103 / In this study, we were to detect of environmental hormone by the only male of tilapia (Oreochromis niloticus), an important species for Taiwan aquaculture. We treated the juvenile of tilapia in 17β-estradiol (E2) and 17α-methyltestosterone (MT), and analyze the mRNA expression of dmrt1, cyp19α and vtg by Q-PCR. The dmrt1 mRNA of gonad was significantly reduced by E2 at 7.2 μM, but has no significant difference of the control group. They have dose dependent of vtg...

Jau-Huan Chen, 陳昭寰

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / Activin is a reproductive hormone and belongs to transforming growth factor β superfamily (TGF-β). It has been shown activin can simulate FSH and GnRH secretion, and gonadal steroidogenesis in mammals. In present study, a 1959 nucleotides of activin βA cDNA by RT-PCR method, encoded 458 amino acids was isolated from tilapia (Oreochromis mossambicus), containing a 238 bp 5’ untranslation region (UTR) fragment and a 344 bp 3’ UTR fragment. Activin βA genomic DNA cloni...

Mei-Fang Lin, 林美芳

博士 / 國立臺灣海洋大學 / 食品科學系 / 93 / Abstract The purpose of the study was to estimate vitamin C requirements of juvenile grouper, Epinephelus malabaricus by using L-ascorbic acid (AA) and its four derivatives namely, L-ascorbyl-2-monophosphate-Mg (C2MP-Mg), L-ascorbyl-2-monophosphate-Na (C2MP-Na), L-ascorbyl- 2-sulfate (C2S) and L-ascorbyl-2-polyphosphate (C2PP). The effects of these vitamin C sources on stress and immune responses in grouper were also monitored. The requirement established was 45.3 mg...

Jhih-Jie Tsai, 蔡志杰

碩士 / 中原大學 / 奈米科技碩士學位學程 / 101

Hong-Jie Lin, 林宏傑

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / Progranulin (PGRN) is a multi-functional growth factor in regulating cell proliferation, tumorgenesis, anti-apoptosis, neuron survival, anti-inflammation, would healing and development. Only one progranulin (pgrn) gene encoding PGRN composed of 7 granulin (GRN) units with 12 conserved cysteines exists in mammalian genome. Progranulin undergoes proteolysis by neutrophil serine protease into small GRN peptides to induce proinflammatory cytokine IL-8. In contrast to ma...

Chien-Wei Liu, 劉千維

碩士 / 國立臺灣大學 / 漁業科學研究所 / 101 / High economic benefits make groupers important aquacultural species in Taiwan. However, the disease caused by grouper iridovirus (GIV) has resulted in economic losses due to high mortality in grouper culture. Therefore we take Epinephelus coioides which easily raised as our model to study the interactions between groupers and GIV. In 2012, Liu and Wu injected poly(I:C), GIV and LPS into orange-spotted groupers and discovered the expression pattern of CD9 resembling ...

李河慧

University of Macau / Faculty of Social Sciences and Humanities / Department of Chinese

HongYou Chang, 張鴻猷

碩士 / 國立中興大學 / 獸醫微生物學研究所 / 88 / The aims of this instigation are to study the effect of five disinfectants, iodorin-200, caflon BKC 80, formalin, malachite green, and SCI-62, on the Staphylococcus aureas subsp. aureus., Streptococcus sp., Aeromonas hydrophila, Edwardsiella tarda, Escherichia coli, Pseudomonas anguilliseptica, and Vibrio anguillarum from the soft-shell turtle and tilpia . At the same time, the acute toxicities of the above disinfectants to the soft-shell turtle and tilapia were st...

Shinn-Shuenn Tzeng, 曾鑫順

博士 / 國立海洋大學 / 食品科學系 / 90 / Cathepsins B and L were considered to be the most active cysteine proteases and revealed high MHC-degradation activities at 40-75°C, in fillet and surimi made from chum salmon, mackerel and/or pacific whiting. They were very difficult to remove during surimi processing and also very stable on frozen storage, which consequently caused gel softening during surimi-based product process. Preincubation of surimi at 20-60°C before heating at 90°C substantially lowered the ge...

kuei-ying Lin, 林桂英

碩士 / 國立海洋大學 / 食品科學系 / 90 / In search of the growth and the immunity promoting substance from food components. We used mammalian cell lines with serum-free medium under animal experiment model. We reported here with the growth and the immunity promoting substances from fish species. Fresh material were homogenized and then boiled at 100℃ for 30 mins. After centrifugation, the supernatant was further concentrated by vacuum evaporation. Through 30%-50% ammonium sulfate saturation precipitation (WG3...

陳慧如

碩士 / 國立海洋大學 / 水產養殖學系 / 90 /

Yong Feng Qian, 錢永峰

碩士 / 國立海洋大學 / 水產生物技術研究所 / 90 / A full-length cDNA clone of 803 bp encoding a putative copper / zinc-superoxide dismutase ( Cu/Zn-SOD ) from malabar rockcod ( Epinephelus malabaricus ) was cloned by PCR approach. Nucleotide sequence analysis of this cDNA clone revealed that it comprises a complete openreading frame coding for 154 amino acid residues. The deduced amino acid sequence showed high similarity (53~91%) with the sequences of the Cu/Zn-SOD from other species. Computer analysis of the res...

De-Feng Weng, 翁得峰

碩士 / 國立海洋大學 / 水產生物技術研究所 / 89 / Abstract A full-length cDNA clone of 774 bp encoding a putative copper / zinc-superoxide dismutase ( Cu/Zn-SOD ) from red porgy (Pagrus major ) was cloned by PCR approach. Nucleotide sequence analysis of this cDNA clone revealed that it comprises a complete epenreading frame coding for 154 amino acid residues. The deduced amino acid sequence showed high similarity (53~91%) with the sequences of the Cu/Zn-SOD from other species. Computer analysis of the r...

Hsuan-Ching Ho, 何宣慶

博士 / 國立臺灣海洋大學 / 海洋生物研究所 / 98 / The results of present study include three topics: review of all Indo-Pacific genera; provide a hypothesis of phylogeny of all genera; and provided the biogeography of family Ogcocephalidae. Seven genera with 55 species are recognized from the Indo-Pacific Ocean, including 7 species in Coelophrys; 14 species in Halieutopsis; 1 species in Dibranchus; 4 species in Solocisquama; 15 species in Halieutaea; 6 species in Halicmetus; and 8 species in Malthopsis. Totall...

Lim Chin Chew, 林健秋

碩士 / 國立臺灣海洋大學 / 食品科學系 / 98 / Two experiments were conducted to quantify the optimum dietary iron (Fe) requirements with organic iron amino acid complex for juvenile tilapia (Oreochromis niloticus × O. aureus), and its effects on immune responses and disease resistance. In experiment I, iron amino acid complex was supplemented to the basal diet at 20. 40, 70, 150, 200 and 400 mg Fe/kg diet, providing 25.87, 40.61, 78.50, 188.27, 219.12, 458.87 mg Fe/kg diet, respectively. Basal diet without Fe su...

Chia-Liang Liu, 劉家良

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 98 / The purpose of this study was to assess the effects of using trimethprim to enhance sulfamonomethoxine, to decrease the dosage used in curing disease and examined it’s residues in the muscle of hybrid tilapia. The result in the experiment shows that the sensitive of trimethoprim, sulfamonomethoxine, and trimethoprim-sulfamonomethoxine to A. hydrophila, E. tarda, Streptococcus sp., V. carchariae, V. alginolyticus are 275 μg/ml, 130μg/ml, 25μg/ml in turn. The trimet...

Kuan-Huang Liu, 劉冠宏

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 98 / The tilapia, a gonochoristic fish with an XX/XY sex-determining system (GSD), provides an excellent model for studing gonadal sex differentiation controlling by temperature (TSD = Temperature Sex Determination). In this study, we report the effects of the temperature during the period of sex differentiation on the growth rate, sex ratio and the gene expression of the Mozambique tilapia (Oreochromis mossambicus). Based on lethal temperature 50 analysis, the toleran...

Yen-Yu Lai, 賴彥佑

碩士 / 淡江大學 / 生命科學研究所碩士班 / 98 / Abstract: PART i : von Hippel-Lindau Disease is a rare condition associated with familial cancers. It is due to a mutation of the VHL tumour suppressor gene at chromosome site 11q13, 3p26-p25. VHL can degrade hypoxia-inducible factors (HIF1a, HIF2a) in the presence of oxygen, thus regulating a lot of hypoxia responsive genes indirectly. pVHL forms a complex with elongins B and C, Cullin2, Rbx1, thus regulating RNA polymerase II and inactivating transcription. After ...

Hsi-Chu Peng, 彭熙竹

碩士 / 淡江大學 / 生命科學研究所碩士班 / 98 / Part1.Acetaminophen(AAP) is widely used as an analgesic and antipyretic drug for decades. In mammals, over-dosed of AAP leads to liver and kidney damages but the toxic effects of AAP is little known during embryogenesis. Here, we used a green fluorescenct kidney line, Tg(wt1b:eGFP), as a model to observe the APAP-induced nephrotoxicity dynamically. We carried out a series of exposure experiments with different concentrations (2.25, 22.5, 45mM), durations (12, 24, 36...