Jou-Ping Hsu, 許柔平

碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 / Abstract High concentration of zinc was found in the digestive tract tissue of common carp which was not seen in other fish species. A zinc-binding protein with a molecular weight of 43kDa located on the plasma membrane of the connective tissue cells was responsible for the high zinc in common carp. Using the “carp zinc-binding protein” as antigen, polyclonal antibody of the protein was prepared. An immunochemical assay method using the antibody against the “carp zi...

Hou-Chu Yin, 殷厚珠

博士 / 國立臺灣海洋大學 / 生物科技研究所 / 97 / Abstract Cytochrome P450 1B1 （CYP1B1） is a heme-containing monooxygenase that metabolizes various polycyclic aromatic hydrocarbons （PAHs） and arylamines, as well as retinoic acid and steroid hormones. Here we presented that zebrafish CYP1B1 is constitutively expressed in embryonic ocular cells, diencephalons, and midbrain-hindbrain boundary during pharyngula stage. After hatching, the constitutive-type of CYP1B1 transcription is attenuated. TCDD exposure elicited ...

Liang-Chian Liu, 柳良謙

碩士 / 國立臺灣海洋大學 / 海洋事務與資源管理研究所 / 97 / In recent years, enhancing resource for marine recreation has become one of the major objectives for artificial reef deployment. In Penghu area, artificial reefs (ARs) have been emplaced since 1974. Up to now, thirteen ARs sites and three warship sites were established, to construct numerous fishing grounds. In the future, apart from fishery yield, recreation value would be an important entry in assessing the effectiveness of ARs. As the recreation value of ar...

陳凱如

碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 /

Ling-Jyun Hunag, 黃凌均

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 97 / During embryogenesis, RNA-binding proteins (RBPs) play crucial roles in the control of gene expression and post-transcriptional regulation of RNAs. The PUF protein family consists of highly conservative proteins that also belong to RBPs. PUF proteins have been discovered in a wide range of species, including yeast, C. elegans, D. melanogaster, M. musculus, and humans. Asides from PUM1 and PUM2 of the PUF family, genetic sequencing of vertebrates reveals two additi...

Jie-Ting Chen, 陳玠廷

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 96 / The genus Priolepis Valenciennes, 1837 belongs to a group of small marine benthic gobies. The phylogenetic hypothesis established in previous research suggests that the presence of transverse rows of sensory papillae is regarded as plesiomorphic feature, and remaining species without those papilla pattern can be separated into two main groups. This research examine the systematics of Priolepis species from Taiwan, and reconstruct molecular phylogeny with partial ...

Pei-Ran Liu, 劉沛然

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / During embryogenesis, the processes of cell differentiation, organogenesis and response to environmental stimulation are all controlled by serious gene regulation system. The bHLH-PAS (Per-AhR/Arnt-Sim) protein family plays important roles in vertebrate development and xenobiotic metabolism. In the bHLH-PAS mediated pathway, Arnt protein plays as a central role, which can dimerize with other bHLH-PAS (such as AHR, HIF, and SIM) to regulate a variety genes. Previous...

Hung-Yen Chen, 陳宏彥

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / The main goal of my thesis is to study the regulatory mechanism of anterior gradient 2 (Agr2) gene expression in zebrafish. At the begining, I used Ensembl Genome Browser to search and download zebrafish Agr2 upstream/promoter DNA sequence, used Promoter scan to predict potential promoter and TATA box regions, used Family Relations II to search for conserved regions of Agr2 upstream/promoter DNA among different species, and used Matinspector of Genomatix website to...

Zhi-Hao Wei, 魏智浩

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 /

Chun-Cheng Hou, 侯君政

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 /

Yi-Ting Wu, 吳儀婷

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / Zebrafish embryos after 24 hpf were soaked in 2 μM β-Lapachone and washed away after 4 h. We clearly observed three developmental defects in β-Lapachone-treated embryos. The first, heart became linear with pericardial edema. The secondary, slow circulation with few or no blood cells were detected in 48 hpf β-Lapachone-treated embryos. The third, severe yolk and pericardial edema can be observed after 72 hpf. Results from β-Lapachone affected gene comparison by DNA ...

Mei-Chun Chen, 陳美均

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / The aryl hydrocarbon receptor repressor (AHRR) is a transcription factor belonging to the basic helix-loop-helix PAS (bHLH-PAS) superfamily. The induction of AHRR by various AHR agonists was investigated in mice and rats. In vertebrate, AHR mediates the biological and toxic effects of various environmental pollutants, such as dioxin and polycyclic aromatic compounds. Via AHR activation, a number of target genes, including cyp1a1 and Ahrr, could be induced significa...

Wei-Chih Chien, 簡緯志

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 96 / The purpose of this study is to know the resource of moray eels in the southeastern waters of Taiwan. This research recorded 30 species 6 genera 2 subfamilies 4574moray eels from July 2004 to June 2008. We also took specimens from the sampling fisherman at Changbin, Taitung since July 2004 to October 2007. Total recorded 33 specie 7 genera 2 subfamilies 3247 moray eels from the fisherman. By the basic data of the moray eels, such as amount and weight (W) and total l...

Pei-Shan Chen, 陳珮珊

碩士 / 國立陽明大學 / 環境衛生研究所 / 88 / Vitellogenin, a yolk-precursor protein normally found only in the blood plasma of sexually mature female teleosts and other egg-laying vertebrates, was used as an indicator of exposure of male carp（Cyprinus carpio）to exogenous estrogens. Alkylphenol polyethoxylates（e.g., 4-Nonylphenol（NP）and 4-tert-Octylphenol （OP））are probable estrogenic. The purposes of this study were twofolds：to examine the estrogenic effect of different alkyphenolic compounds to male carp, and t...

Ming-Yean Lin, 林明岩

碩士 / 國立臺灣大學 / 畜產學研究所 / 88 / Abstract In this study, we used a carp pit-1 gene DNA fragment containg POU domain as probe to screen zebrafish POU family gene. We successfully obtained a POU-domain containg gene, oct-1, named clone 3-1 from zebrafish brain DNA library. Computer comparison of the sequence indicated that this sequence was identical to zebrafish oct-1 gene. By using this partcial oct-1 DNA fragment as probe to screen the zebrafish 5''-stretch plus cDNA library, we got two ...

Ching-yi Hsiao, 蕭青毅

碩士 / 國立臺灣大學 / 海洋研究所 / 88 /

Yin Wang, 王尹

碩士 / 國立海洋大學 / 水產生物技術研究所 / 88 /

Fei-Lan Liu, 劉斐蘭

碩士 / 國立海洋大學 / 水產生物技術研究所 / 88 / Abstract When cells are subjected to environmental stress conditions, they rapidly response to synthesize a suite of stress proteins, or referred as heat shock proteins (HSPs). These stress proteins are highly conserved from bacteria to human. Under stress conditions, a number of these stress proteins act as molecular chaperons that enable cells to survive in serious environmental conditions by preventing protein denaturing. In addition, some stress prot...

Huang, Chi-Ruei, 黃啟瑞

碩士 / 國立海洋大學 / 水產生物技術研究所 / 88 /

Kuo-Yu Cheng, 鄭國有

碩士 / 國立海洋大學 / 漁業科學學系 / 88 /

CHiu LIMAN, 邱麗滿

碩士 / 國立海洋大學 / 海洋生物研究所 / 88 / In telestean fishes, the egg surface contained a protein responsible for initial sperm recognition and binding as well as for the induction of the acrosome reaction. The sperm-binding protein was defined as female-specific proteins, which in the extracellular coat and layer or/and the egg plasma membrane. The female-specific proteins are very species-specific. The 97 kDa protein was isolated and purified from the egg surface of Epinephelus malabaricus and assayed as ...

Yu-Hong Lin, 林鈺鴻

碩士 / 國立海洋大學 / 食品科學系 / 88 / The first study was to investigate the utilization of carbohydrate and the protein-sparing effect by different carbohydrates in juvenile malabar grouper (Epinephelus malabaricus). White fish meal of dietary protein was incorporated into the diet providing 50, 46 and 42% of dietary protein. The carbohydrate sources were starch and glucose. After 8 weeks of feeding, weight gain of glucose-fed and starch-fed fish were similar (p>0.05) at each dietary protein level. Weight...

Gwo-Chin Ma, 馬國欽

碩士 / 國立臺灣大學 / 漁業科學研究所 / 87 / There exists a highly conserved region located at -166 to +94 bp between two types of carp rhodopsin genes. Two putative cis-acting elements, BAT-1-like and NRE-like, were found within this region based on sequence alignment with the corresponding regions of rhodopsin genes of other known species. A novel putative Crx binding site, GTAATCC, located at -52 to -46 bp adjacent to the TATA box, was also found. Three oligonucleotide probes, named cBAT-1, cNRE and cCSE, we...

Shih-Ling, Huang, 黃世鈴

博士 / 國立臺灣大學 / 動物學研究所 / 87 / This paper describes the epizootiology of a disease of tilapia with clinical signs including the presence of white nodules and microscopical granulomatous formations. The diseased fish showed splenomegaly with diffusion of numerous white nodules. The most severe lesions are presented in the spleen and anterior kidney. Morphological, biological and biochemical characteristics of microorganisms isolated from the diseased tilapia were examined and classified by Baird-P...

Chu-Hsuan Chen, 陳筑瑄

碩士 / 國立臺灣大學 / 生化科學研究所 / 87 / Nephrosin is a newly discovered member of the astacin family, a group of secreted or membrane metalloproteinases found in the 1990s. It is the first astacin enzyme found in lymphohematopoietic tissues (kidney, head kidney, and spleen in fish). Carp nephrosin in its secreted form contains 198 amino acid residues whose predicted relative molecular weight is 23k. Four putative heparin binding motifs can be identified by which secreted nephrosin may be localized to the h...

許惠真

碩士 / 國立海洋大學 / 水產生物技術研究所 / 87 /

Chun-Ming Lin, 林俊銘

碩士 / 國立海洋大學 / 漁業科學學系 / 87 /

Li Chia-Yun, 李佳芸

碩士 / 國立海洋大學 / 食品科學系 / 87 / The aim of this study was to investigate the optimum conditions for the recovery of native proteins from freeze-denatured fish muscle using the freeze-dried crude NADPH-sulfite reductase produced by Escherichia coli. It was found that the optimal culture condition for Escherichia coli (CCRC 11634) was in broth of 4.0% sucrose, 0.4% yeast extract and 0.4% tryptone at pH 7.5 with shaking (100 rpm) at 37C for 18 hr. The cells were harvested by centrifugation and the cr...

Lin Mei-Hsin, 林玫欣

碩士 / 國立海洋大學 / 食品科學系 / 87 / The mackerel meat and viscera were hydrolyzed by autolysis and with the addition of Protease A and Protease N. The pH value of meat hydrolysates decreased and then increased during hydrolysis for 24 hr. However, the pH of the viscera hydrolysates decreased gradually. The NH3 of all hydrolysates increased with the hydrolysis time. The soluble proteins of enzymatic hydrolysis of the meat increased and then decreased during hydrolysis, but those of the viscera hydrolysate...

Yeh-lungchi, 葉龍吉

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / Grouper farming is an important industry in Taiwan. A farm survey was conducted to analyze the economics of production and marketing system. The research indicated that the fry cost is a major production cost. To reduce the fry cost is therefore a major issue. The analysis of concentration ratio(CR)was reported at CR4=65.5%，which revealed that the grouper marketing is a tight oligopoly market. As a result，a cooperate system of production and marketing is suggested to ...

林俊麒

碩士 / 國立海洋大學 / 水產養殖學系 / 87 /

陳榮芳

碩士 / 國立海洋大學 / 水產養殖學系 / 87 / Pichia pastoris system has potential for very high level production of foreign protein. In this experiment, we expressed it as the eel growth hormone and the expression of recombinant eGH was produced after methanol induction in 24 hours. We tried to use the ammonium sulfate instead of YNB and we found that it is feasible to achieve the goal of economical cost. Biologic activity of recombinant growth hormone was determined by feeding Trionyx Sinensis. Over ...

Shin-Yan Chen, 陳新言

碩士 / 國立清華大學 / 生命科學系 / 89 / Onychostoma alticorpus（Oshima, 1920）is an endemic species in Taiwan, which distributes in Kaoping River, Hsiukuluan River, and Peinan River, mainly the south and east of Taiwan. This study also analyzes two other species. Onychostoma sima（Sauvage et Dabry, 1874）, distributes in Mainland China, which shares many morphological similar characters with Onychostoma alticorpus. Another taxonomic closed species, Onychostoma barbatulus（Pellegrin, 1908）, distributes in Taiwan a...

Yu-Ping Poh, 繁玉萍

碩士 / 國立清華大學 / 生命科學系 / 89 / Taiwan Island locates at the margin of Eurasia plate. Lots of species have dispersed from the mainland of Eurasia to Taiwan after the formation of the island. The main biota of Taiwan has formed consequently. Central Mountain Range has kept uplifting for about 5 million years since the appearance of Taiwan Island. The uplifting of Central Mountain Range significantly affects the surface of Taiwan and certainly shapes the biota composition. Therefore, many re...

Ching-Shui Ke Wang, 柯清水

碩士 / 國立中山大學 / 海洋資源學系研究所 / 89 / ABSTRACT The mortalities for aquaculture animals in a newly established pond tend to be high. The phenomenon can be described as the “new pond syndrome”. The main reason for the phenomenon is still unclear. However, it is suspected that the accumulation of ammonia due to insufficient nitrifying bacteria in the system may have been the cause. The purposes of this thesis are to test the theory and to rectify the problem by introducing a nitrifying trickling filtrat...

Li-Hsueh Wang, 王立雪

博士 / 國立中山大學 / 海洋資源學系研究所 / 89 / Neurotransmitters are widely distributed in the central nervous system of tilapia during development. In the present study, the effects of gonadal steroids, temperature, and neurotransmitters on the development of central neurotransmitter systems in tilapia, Oreochromis mossambicus, were investigated. Exogenous E2 before 10 days posthatching resulted in an inhibitory effect on the activity of central 5-HT system via decreasing TPH activity and increasing MAO acti...

Wei-Fen Sheu, 許偉芬

碩士 / 國立中興大學 / 植物學系 / 87 / Hydrogenase activity in N2-fixing cyanobacterium Anabaena CH1 and CH2 have different physiological reactions in different environments, The growth rates of Anabaena CH1 and CH2 reduces with the decreasing light intensities in different illumination degree. In the dark, both Anabaena CH1 and CH2 do not grow. The hydrogen formation activity of the extract of Anabaena CH1 were lower in 25℃ than 4℃. The hydrogen formation activity of the extract of Anabaena CH2 ...

Wen-Teng Chen, 陳文騰

碩士 / 國立中興大學 / 食品科學系 / 87 / Tilapia (Orechromis niloticus) was used as raw materials to investigate the changes in freshness and sanitary quality during transportation. The applicability of the fish in different quality was also investigated. Tilapia stored at 0℃ was regarded as control to detect the sanitary quality index for muscle properties including gel forming ability, color, activity of actomyosin Ca-ATPase, and the number of microorganisms. The results obtained were as follows: ...

Yang-Kai Cheng, 鄭揚凱

碩士 / 國立中興大學 / 食品科學系 / 87 / The provincially aquacultured tilapia was used as materials to prepare a smoked dainty product. Tilapia fillets immersed with seasonings were semi-dried by hot-air and subsequently dried by smoking. The appropriate seasonings and processing process were investigated via hedonic sensory evaluation. Quality of the smoked dainty product was also determined. The results obtained were as follows: 1. Moisture content, water activity, salt content, and labelled...

Chi-Cheng Yu, 游啟政

碩士 / 國立中興大學 / 食品科學系 / 87 / Actomyosin（AM）extracted from ordinary muscle of tilapia（Orechromis niloticus） was pressurized at 500~3,000 atm for 10-60 min (4℃）or heated at 25~95℃ for 10-60 min. Changes in chemical, conformational, physiological and thermal gelation characteristics of the treated AM were measured. The results obtained were as follows: 1. Surface hydrophobicity and surface sulfhydryl groups of AM increased with the increase of the treated pressure. The ...

Shinn-chuang Lai, 賴信川

碩士 / 華梵大學 / 東方人文思想研究所 / 87 / Title of thesis: The Research of Gyo-san-sho-myo-shu-- The Investigation of Development of Chinese Buddhism Ritual Music Name of Institute: The Institute of Oriental Humanities of Hufan University Graduate date: June 1999 Degree conferred: Master Student: Shinn-chuang Lai 賴信川 Advisor: Prof. Wan-nai Chen 陳萬鼐 Prof. Kwong-yim Ho 何廣棪 Abstract ...

Juang, Bi-Tzen, 莊碧簪

碩士 / 台北醫學院 / 細胞及分子生物研究所 / 84 / Snakehead fish (Channa maculata) is a bimodal breathing fish, able to acquire oxygen from water as well from air directly. Hemoglobin of this fish may therefore be an excellent model for illustrating the functional aspect of the structural evolution in respiratory protein. To date, however, neither the structure nor the oxygen transport properties of this respiratory hemoglobi...

Tsai, Hui-Hsin, 蔡蕙歆

碩士 / 國立陽明大學 / 微生物暨免疫學研究所 / 82 /

羅祥雲

碩士 / 東吳大學 / 微生物學研究所 / 82 / From the analysis of the possible relationships between the heritable melanoma formation in the xiphophorine hybrids and the retrovirus, many DNA sequences in xiphophorine genome with homology with the reverse transcriptase gene pol has been found. The proposed characteristics of these DNA sequences were similar with the dispersed repetitive sequences named as retroposon found in the vertebrate. In this srudy, we used these DNA sequences isolated from a cDNA library ...

Mai, Sha Li, 麥莎麗

碩士 / 國立臺灣大學 / 漁業科學研究所 / 82 /

Wang, Ran-Juh, 王仁助

碩士 / 國立臺灣大學 / 食品科技研究所 / 82 /

Huang Yun Li, 黃筠澧

碩士 / 國防大學政治作戰學院 / 政治研究所 / 102 / Diaoyutai Islands, an Inherent Territory of Republic of China which locates in the center of 1st island chain, is the center of western pacific air and ship transport. The significance of its geographical position is obvious. According to the Emery’s Report in 1969, “Diaoyutai Islands” is one of the rare continental shelf with immeasurable gas resource that have not been drilled. Therefore, the territorial sovereignty becomes a dispute in the world. Recently, ...

Po -Yen Chen, 陳柏諺

碩士 / 國立臺灣師範大學 / 生命科學研究所 / 100 / Epithelial Mitochondria-rich cells (MRCs) in fish gills play a critical role in acid-base regulation. In our previous studies, we have found Na+/H+ exchanger (NHE3) and rhesus glycoprotein (Rhcg1) were involved in the acid and base excretion at the apical membrane of MRCs in medaka (Oryzias latipes). To date, little information is known on the acid-base regulation as fish faced the hypercapnia environment. In the present study, a scanning ion-selective electrode t...

Lo, Yu, 羅聿

碩士 / 國立清華大學 / 分子與細胞生物研究所 / 102 / Freshwater fish in Taiwan has high biodiversity, however, has been imperiled due to hydraulic engineering, development of water source, pollution, and abuse of pesticide and introduced species and global climate change. Three taxa have been presumed extinct and 18 are endangered among 69 native freshwater fish in Taiwan. We confirm 1960 is the last recorded year of observation of the endemic species, Salanx acuticeps Regan, 1908, and suggest the conservation stat...

Hsieh Yuan Hsin, 謝元信

碩士 / 國立高雄海洋科技大學 / 漁業生產與管理研究所 / 101 / With two stages of questionnaire surveys, this study made it clear of the point of view of how the industry-government-academy feels about the artificial reefs (ARs) which were set by government. There were 45 items in the first stage’s questionnaire, and only 36 were left by statistical item analysis. Seven hundred and twenty eight questionnaires were mailed back and used to analyze the view point of the industry-government-academy on setting ARs. The K...