Chuang,Ming-Hong, 莊明��

碩士 / 國立臺灣大學 / 生化科學研究所 / 82 / Gamma-Crystallin is a ubiquitous lens protein of most vertebrate eye lenses and the major component in lenses of fishes and many mammals, especially during embryonic and neonatal stages. To date it seems to be absent in the birds and some reptiles. To facilitate the cloning and sequencing ofγ- crystallin gene,cDNA was constructed from poly(A)+RNA of shark lenses (Chiloscyllium c...

Hsiao,Pei-Wen, 蕭培文

碩士 / 國立臺灣大學 / 生化科學研究所 / 82 / CDC2蛋白激脢的活化與高等動物細胞週期的分裂期進行有關。而在動物組 織中，已知至少存在六種結構和 CDC2相似的CDK分子，配合著不同的 cyclin擔任調節細胞週期的不同角色。利用RT-PCR在鯉魚組織找到數種類 似cdk的DNA片段，並在卵巢組織 cDNA 基因庫篩選出一個全長1.3Kb的 cDNA clone，可轉譯一個含 302個氨基酸的CDC2 蛋白激脢，此蛋白激脢 和各種真核生物的CDC2比較，相同的氨基酸約佔80%。為了研究 CDC2的基 因調控，我們進一步從鯉魚染色體基因庫中篩選出含 cdc2基因的phage clone，並定出長約 5Kb 的部份DNA序列，此序列涵蓋cdc2基因的啟動子 區域和cdc2基因的蛋白質轉譯區，分析 cdc2 基因的啟動子區域DNA 序列 ...

Li, Qing Quan, 李清泉

碩士 / 國立海洋大學 / 漁業科學研究所 / 82 /

Yang, Dun Yi, 楊惇懿

碩士 / 國立海洋大學 / 水產養殖研究所 / 82 /

Li, Xin Mei, 李欣玫

碩士 / 國立海洋大學 / 水產食品科學研究所 / 82 /

Zeng, Wen Peng, 曾文鵬

碩士 / 國立海洋大學 / 水產食品科學研究所 / 82 /

Li, Jia Zhan, 李嘉展

博士 / 國立海洋大學 / 水產食品科學研究所 / 82 /

Lin, Mei Feng, 林美鳳

碩士 / 國立海洋大學 / 水產食品科學研究所 / 82 /

Hsiao-Hui Chuang, 莊曉慧

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 95 / After Taiwan joined WTO, the import fish customs duty was greatly reduced. This created a large amount of domestically cultivated fish. However Taiwan has had more than hundred years of history in fishery cultivation. Grouper has more than 20 years cultivation history in emerging cultivation fingerlings. It greatly raises the technology to achieve complete breeds from the production of roe, the fry breeds, to the juvenile fish length, and adult fish, and again...

Hang Wang, 王涵

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Abstract Two subsequent growth trials were conducted to elucidate the effects of dietary nucleotides (NT), including inosine monophosphate (IMP), adenosine monophosphate (AMP), guanosine monophosphate (GMP), uridine monophosphate (UMP) and cytidine monophosphate (CMP), supplementation on growth and immune responses of juvenile grouper, Epinephelus malabaricus. Basal diet containing 0.5, 1.0, 1.5 and 2.0 g/kg diet mixture of IMP, AMP, GMP, UMP and CMP (1:1:1:1:1) (mix...

Ting-Yi Ko, 柯亭伊

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 95 /

Tz-Hau Kuo, 郭子豪

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / A research was carried out to evaluate factors that affect the profitability of the Taiwanese eel industry, focussing on culture system, input costs, and drugs additives. The factors were cross ahalysed by examining eel fry production in Asia and considering the trade of eel to Japan, in total eel production and price, and the interaction between the two, and other factors. A SWOT analysis to compare the competetiveness of the eel industry between Taiwan & China yie...

Jyun-Cing Cao, 曹俊清

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / Abstract The objectives of the study were to investigate the effects of different salinities and chemical stimulus in CRH, CRHR, HSP70, IL-1β and Na+/K+-ATPase gene expressions in vivo and in vitro gill of black porgy (Acanthopagrus schlegeli ). In vivo experiment, CRH, CRHR, HSP70, IL-1β and Na+/K+-ATPase gene expressions were compared in different tissues. In vitro gill culture, CRH had more effects in the gill expression of genes than dexamethasone. The results ...

Yoni Alfonzo Cardona Torres, 優尼

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 95 / A bioeconomic analysis of tilapia (Oreochromis spp) farming in Taiwan was carried out with the aim to evaluate the economic performance of the Taiwan tilapia grow-out sector. For the study a total of 46 farms from different counties (Taoyuan County, Hsinchu County, Tainan County, Chiayi County, Pintung County and Kaohsiung County) were selected to be evaluated, based on the importance that these counties represent to the total tilapia country production. Multivaria...

Chun-An Chen, 陳俊安

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Abstract Cobia skin was hydrolyzed by protamex (PX), Prozyme 6 (P6) and Protease N (PN) for 2 or 5 h. The inhibitory effects of cobia skin hydrolysates against angiotensin I converting enzyme (ACE) and antihypertensive effect were investigated. The highest value of peptide content (400mg/g) of hydrolysates was prepared by PN hydrolysis for 5 h (PN5). PN5 hydrolysate showed the lowest IC50 on ACE being 0.728 mg powder/mL, and PX5 hydrolysate (IC50=0.820 mg powder/mL)...

Yi-Chun Lee, 李依純

碩士 / 國立臺灣大學 / 漁業科學研究所 / 96 / In mammal, bicarbonate transporters play an important role in pH regulation, and ten isoforms are grouped into the solute carrier 4 (SLC4) family. Some SLC4 members have been proposed to be associated with ion regulation and acid-base balance in fish, but no convincing evidence is available to support so far. The present study used zebrafish as a model to examine the role of SLC4 members in fish ion regulation and acid/base balance mechanisms. Ten genes of the zebraf...

Yao-Tsung Hsu, 許_耀琮

碩士 / 立德管理學院 / 應用資訊研究所 / 95 / Along with the increasing prevalence of internet, people need internet which has universality for shopping and gaming anytime and anywhere. New crime types are founded in the same time. One of the new crime types is internet phishing and it has affected people most. Phishing uses social engineering techniques for feinting and cheating target’s privacy information. Using phishing mail, websites and chatting technique makes users be mislead to divulge privacy informat...

Wei-Hung Li, 李偉弘

碩士 / 明志科技大學 / 機電工程研究所 / 96 / Due to the characteristic of the ultra-wide view angle of fisheye lenses, the range of the application of the fisheye lens would be broadly. However, all the fisheye lenses suffer from a serious barrel distortion. How to restore the problem will be the main topic for us. That is, we had already developed a rectify system which can successfully find the laws of all kinds of the fisheye lens in forming the images. Although different fisheye lenses have different projec...

Tzu-hung Huang, 黃子鴻

碩士 / 國立成功大學 / 系統及船舶機電工程學系碩博士班 / 96 / In the field of fluid mechanics, bio-inspired propulsion is important.The main purpose of this thesis is to investigate the possible application of bio-inspired propulsion in ships. Therefore, we study the propulsion method of bio-inspired fin by investigating the effect of the changes of geometry of fin to thrust trend. In our experiment, we define thin-triangle fin system. According to the changes of geometry, we compare the thrust trend of fin by using NBR...

Hung-du Lin, 林弘都

博士 / 國立成功大學 / 生命科學系碩博士班 / 96 / The primary fresh water family Cyprinidae represents the richest and diversification ichthyofauna in Taiwan and South China. Cyprinid fishes dwelling in island-like habitats provide an ideal model for phylogeographical studies. The present and historical connections among rivers are the basis of the interpretation of the observed genetic relationships among populations. We examined the phylogeographic structure of eight cyprinid fishes, including Ahyocypris kikuchi...

Lu-Peng Wang, 王律棚

碩士 / 國立東華大學 / 海洋生物多樣性及演化研究所 / 97 / Pomacanthidae are common coral reef fishes widely distribute in reef coasts of the world. They are of commercial values being captured as marine ornamental fishes due to the varied and attractive colorations. Most of the past studies were related to the reproduction and behavior of the fish, limited knowledge was obtained for their life history. An understanding of biology and ecology of the fish would be beneficial to the assessment of wild population and coul...

KUAN-YING JIAN, 簡寬穎

碩士 / 中山醫學大學 / 生化暨生物科技研究所 / 102 / Scratch gene play an important role in the regulation of neuron development. Recent study showed that downregulation of Scratch gene in mouse model impaired CNS differentiation. In present article, we want to examine whether knockdown of Scratch gene also cause the deficient in neurogenesis via zerbrafish model. According to the phylogenetic tree suggested zerbarfish scrt gene is high conserve with human and mouse counterpart. Based on PCR analysis and in situ hy...

I-Tao Sun, 孫亦韜

碩士 / 淡江大學 / 國際事務與戰略研究所碩士在職專班 / 102 / Safeguarding the integrity of national territory has always been the prime concern for all governments in the world. Facing territorial dispute, almost no country shall concede the loss of any piece of land; accordingly, any international territorial dispute must have resulted from a conflict on competing sovereignty positions between rivalry nations. There are three primary causes for such territorial conflicts, including competing claims for sovereignty, st...

Yu-Tien Chou, 周雨田

碩士 / 淡江大學 / 國際事務與戰略研究所碩士在職專班 / 102 / Kenneth Waltz’s proposition of structural realism has been used as the theoretical basis. The author explores China-Japan’s strategic competition over the sovereignty of Diaoyutai Islands during 2010-2014. This paper will analyze the China-Japanese Diaoyutai issue by the balance-of-power theory. The purpose of this thesis is discussed Sino-Japanese strategies over the Diayutai disputes: (1)What are the main points of China government and Japan government in ...

Kuan-Chieh Peng, 彭冠傑

碩士 / 國立臺灣大學 / 漁業科學研究所 / 97 / For the purpose of understanding the application of antimicrobial peptides in aquaculture, transgenic zebrafish expressing epinecidin-1, was developed and reported here. First, we cloned zebrafish mylz2 promoter for this purpose. To characterize the activity of mylz2 promoter, various fragments of the mylz2 promoters were analyzed using firefly luciferase transient expression assay, in which maximum promoter activity was found in the 2.5 kb fragment. Besides, the 2.5...

Ren-Xiang Wang, 王任翔

碩士 / 國立臺灣大學 / 動物學研究所 / 97 / Nervous necrosis virus (NNV) is a positive single-stranded RNA virus. NNV infection was reported to induce the loss of mitochondria membrane potential. Autophagy is a unique membrane traffic system for engulfing long-lived proteins and organelles to maintain homeostasis of cellular energy during starvation. This research first reported the interaction between NNV and autophagy in fish cells. After transfection of pEGFP-LC3 plasmids, fluorescence of autophagosomes was ...

Kun-Chan Tsai, 蔡昆展

碩士 / 國立臺灣大學 / 動物學研究所 / 97 / Betanodaviruses, the causative agents of viral nervous necrosis disease, are classified into 4 genotypes, including red-spotted (RG), striped jack (SJ), barfin flounder (BF) and tiger puffer (TP) nervous necrosis virus (NNV). The aim of this study is to screen NNV in the wild fish collected from different aquatic area around Taiwan, and investigated the molecular phylogeny and epidemiology of those NNV isolates. Nested RT- PCR was applied for the screening of T4 segme...

Yu-Hsun Chang, 張譽薰

碩士 / 國立臺灣大學 / 食品科技研究所 / 97 / Chinese soft-shelled turtle (SST) is a traditional Chinese medicine, and was also confirmed that possessed various kinds of physiological functions. The objectives of this study were to produce anti-hypertension effect of SST powder hydrolysate digested by papain. SST powder was hydrolyzed under various conditions of hydrolysis time and enzyme-to substrate ratio (E/S, %) according to a response surface methodology (RSM) 2 factors rotatable central composite design, a...

Chun-Wei Lin, 林浚緯

碩士 / 國立臺灣大學 / 動物學研究所 / 97 / Cofilins are members of actin-depolymerizing factor (ADF)/cofilin protein family that regulate actin treadmilling and the extension of pseudopods in motile cells. To study cofilin functions in gastrulation cell migration, we have isolated cofilin 1 (cfl1) from zebrafish genome, and its expression and functions have been examined. By RT-PCR and whole-mount in situ hybridization, cfl1 has been found to be ubiquitously expressed during early embryogenesis. We have also d...

Yi-Chung Chen, 陳宜群

博士 / 國防醫學院 / 生命科學研究所 / 97 / Synuclein was first indentified in cholinergic presynaptic vesicles from Torpedo californica. It has been kwon that human synuclein family consists of α-, -, and -synucleins. Only α-synuclein has been implicated in the pathogenesis of neurodegenerative diseases, for example, parkinson’s disease, dementia with Lewy body, multiple system atrophy and Alzheimer disease. -Synuclein has the function in the vesicle trafficking. -Synuclein plays an important role in tumor...

林明杰

碩士 / 國立臺灣師範大學 / 音樂學系 / 96 /

Yu -Cheng Lin, 林俞呈

碩士 / 國立臺灣海洋大學 / 應用經濟研究所 / 94 /

Chieh-Yu Pan, 潘婕玉

碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 94 / Abstract Pleurocidin, an antimicrobial peptide, plays important roles in fish to protect themself from microbial infection. Using a plaque screening assay, we isolated cDNA and genomic DNA clones encoding a novel pleurocidin wsa isolated from grouper (Epinephelus coioides) cDNA library and genomic DNA library. The pleurocidin cDNA consists of 519 bp with an open reading frame of 201 bp. The deduced protein has 67 amino acid residues, a calculated molecular...

Chih-Yang Lin, 林志洋

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / The process of smoked shark product includes steaming of shark fillet and smoking with sugar or sugarcane. The chemical composition, freshness, quality and sanitation of commercial smoked shark products were investigated. The changes in quality and flavor of smoked shark during storage at 25oC and 4oC were also studied to evaluate the shelf life of the product. The moisture contents of commercial smoked shark ranged from 75.7 to 82.0%, protein 17.6 to 21.1%, ash 0.97...

Chein-Tso Chan, 詹健佐

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / Abstract We have previously cloned a zebrafish KLF4a cDNA, which encodes 396 amino acids. Amino acid sequence comparison showed that zebrafish KLF4a shares 65.8％ sequence similarity with mammalian KLF4. KLF4a is a meternal mRNA, it was expressed in the eyes, otic vesicle, midbrain-hindbrain boundary (MHB), ventricular zone at 24 hpf (hour post fertilization), and was expressed in the liver, exocrine pancreas, intestine between 48 to 96 hours of development. Pre...

Chun-Yen Tsai, 蔡君彥

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / Previously we obtained a zebrafish cdx2 cDNA and named it cad2 from a zebrafish cDNA library screening. Cad2 encodes a 255 amino acids long polypeptide that containing a caudal like activation domain near N-terminal region and a 60 amino acid homeobox DNA binding domain near the C-terminus. In Pei-Yi Cheng master thesis, embryos that have been injected with antisense morpholino oligomer-1 (cad2-MO1), which complemented with the 5’-untranslated region including the ...

Yu-Xuan Weng, 翁玉宣

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / Abstract This study investigated the immunological protection of experimental divalent vibrio vaccines on the juvenile grouper (Epinephelus sp.). The measurements of immuno-response in the adults included agglutinating activity of fish antisera, serum bacteriocidal assay and rocket immunoelectrophoresis assay. The V. alginolyticus strain S4Y and V. carchariae strain EmI 82 KL, originally isolated from moribund groupers were passed in the fish to increase its viru...

Yi-Hui Chen, 陳怡慧

碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 94 / Abstract The objectives of the this study were to investigate the possible roles of corticotropin-releasing hormone (CRH), corticotropin-releasing hormone receptor (CRH-R1) and Interleukin-1β (IL-1β) in the gill of black porgy. We designed acute salinity change, cortisol oral administration and in vitro gill culture experiments to study CRH, CRH-R1 and IL-1β gene expressions in gill and hypothalamus. The quantitative PCR measurement of the respective genes was ...

Han-Hieh Kuo, 郭瀚介

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 94 / The ZP3 is a female specific protein on the surface of eggs for binding the sperm in reproduction. The purpose of this thesis is to gate ZP3 gene sequnce by using genatic clonning method, and by using scanning electron microscopy to observe microstructure of egg surface of the tilapia including Oreochromis niloticus, O. aureus and Oreochromis sp.. We tried to identified three kinds of tilapia by cloning ZP3 gene and analyzing similarity of gene and protein sequence...

Wel-Lung Tsai, 蔡維隆

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / Abstract Moray eels are the commercially important fish species in Taiwan. These fish distribute in the tropical and subtropical coral reef district in the world extensively and are carnivorous and edible important species. Otherwise, the food poison incident occasionally occurred when people ate them. Therefore, these fish are commonly recognized as ciguateric species. Recently, one of ciguateric toxins, ciguatoxin, has been isolated from moray eel Gymnothorax java...

Huei-Lin Huang, 黃琳惠

碩士 / 國立臺灣海洋大學 / 食品科學系 / 94 / The purpose of this study was to investigate the effects of dietary fiber addition on the qualities of surimi-based product of threadfin bream (Nemipterus virgatus). The setting treatment at 40℃ significantly (p＜0.05) increased breaking force, breaking point, gel strength, rigidity, lightness and whiteness of surimi-based product as compared to those without setting. The setting period of 30 minutes showed the most effective. The breaking force, breaking point, gel s...

Shing-Yi Yeh, 葉幸宜

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 94 / Abstract Cytochrome P450(CYP) are a superfamily of heme-thiolate containing mono-oxygenases involved in the oxidative metabolism of a diverse range of compounds, including steroid hormone ,fatty acids, and toxicants. Members of CYP1, CYP2, and CYP3 family are responsible for a variety of xenobiotics and drug metabolism. In previously study, it was shown that TCDD could enhance zebrafish CYP1A1, CYP1C1and CYP3A65 transcription during larva stages. It suggests that T...

Hsiao-Wei Kuo, 郭曉薇

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 93 /

Chu-mei Yu, 余茱玫

碩士 / 國立中山大學 / 海洋生物研究所 / 94 / The purpose of this study was for building the data on blood and serum chemistry of grouper ‚ and expect these data can be helpful to know the fish condition in early stages‚ in order to remedy as soon as possible and decrease the loss that caused of fish disease. Three experiments were conducted to establish the data on blood and serum chemistry of grouper. In the first experiment‚ the clinical chemistry including red and white blood cell counts‚ Glutamic oxalacetic...

Tsung-jung Wu, 吳宗榮

碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 94 / Glutamine synthetase (GS; EC 6.3.1.2; L-glutamate ammonialigase) catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. Due to its key role in nitrogen metabolism, including nucleotide, amino acid and urea biosynthesis, the enzyme has been ascribed an extraordinarily long evolutionary history. Thus, GS has been used as a molecular clock to establish phylogenetic relationship between different species. Through the National Center of Bio...

Sheng-hui Huang, 黃升暉

碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 94 / In tilapia , sex determination is controled by genetic and triggered by the environmental factors. Expressed sequence tags ( EST ) derived from the developing tilapia brain is cloned in our lab. The cDNA full length of the gene, F10A83 was cloned. In the present study, F10A83 is a gene with 1526 bp of cDNA sequence, and deduced 176 amino acids of protein sequence. F10A83 was overexpressed as a GFP fusion protein in mouse neuroblastoma Neuro-2a cells. F10A83 is...

Wan-Chi Lai, 賴婉綺

碩士 / 國立中興大學 / 森林學系所 / 94 / This is the first paper to evaluate a natural product used as an antisilverfish agent all over the world. Silverfish (Lepisma saccharina), primitive wingless insects, feed on a variety of materials, including paper, cotton, starch and cereals. It is a general common pest of house. In this study, the essential oils of Cryptomeria japonica were selected to evaluate its effect of repellent and death against silverfish. Before repellency and anti-silverfish assay, the cult...

Yi-Chun Liu, 劉怡君

碩士 / 長庚大學 / 基礎醫學研究所 / 94 / Enterovirus 71 (EV71) is a positive-stranded RNA virus in the genus Enterovirus of the family Picornaviridae. EV71 infection can cause hand, foot, and mouth disease (HFMD), herpangina and associated with fatal pulmonary edema as well as severe neurological complications, including encephalitis, meningitis, and a poliomyelitis-like syndrome in children. Several major outbreaks of EV71 have occurred in recent years in Taiwan. In this study we screened 22 Chinese medic...

Hui-Ju He, 何惠茹

碩士 / 國立臺灣海洋大學 / 應用經濟研究所 / 92 / Taiwan exported frozen roasted eel to Japan successfully since 1968 and the eel production and aquaculture acres in Taiwan had increased rapidly since then. Taiwan had become the largest eel exporting country in the world from 1980 to 1990. Facing the competition from China, Japan had decreased their imports from Taiwan for 24％ in 1979 and had reduced it’s import further in the following years. Facing the domestic consumer behavior is different from Japan, the d...

Shih-Chieh Hung, 洪士傑

碩士 / 國立臺灣海洋大學 / 應用經濟研究所 / 92 / Eel aquaculture plays an important role in the fishery. However, how to move on for eel aquaculture of Taiwan is an issue because of fierce contention. Therefore, this study focuses on the relationship among cost, revenue, and profit of eel bred, trying to analyze the production factors based on Cobb-Douglas cost function, willing to investigate an function that fitted the market according to supply-demand model and 2SLS method, and finally, finding a way to raise ...