Chen, Jian-Chih, 陳建誌

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 99 / The purpose of this study is to establish the process conditions for preparing collagen peptide from seaperch scale and to study its functionality on calcium-binding (Ca-B) activity. Powder samples from fish scale of seaperch were treated with alcalase, flavourzyme, papain, trypsin, and protamex. The collagen peptides obtained were examined and compared with their Ca-B activity. Among them, peptides from fish scales treated with alcalase, flavourzyme, papain sh...

Ya-Wen Hsu, 許雅紋

碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 96 / Escherichia coli Nitroreductase (NTR) is a enzyme that needs prodrugs to induce it. It can not only be used in non-divided or divided cells, but also controls the toxicity and time. When removing the prodrugs, the damaged cells will restore. Thus NTR is a very good tool to study the function and the development of organs. Use Sperm-t, SAMP14, ZP3, and AKAP would be specific in gonad to be the promoters and forming gene sequences. Use microinjection to settle the ge...

Hsiao-Ting Lee, 李筱婷

碩士 / 國立臺灣海洋大學 / 食品科學系 / 96 / In quantifying dietary zinc (Zn) requirements from organic source for juvenile tilapia, Oreochromis niloticus × O. aureus, zinc methionine was added to the basal diet at 0, 5, 10, 15, 20, 30, and 50 mg Zn/kg diet providing the actual dietary value of 1.16, 3.23, 8.62, 14.16, 19.48, 26.15 and 52.04 mg Zn/kg diet, respectively. Each diet was fed to triplicate groups of fish (initial body weight 1.29 ± 0.01 g) in a recirculated rearing system for 8 weeks. Results indica...

Whae-Ling Chuang, 莊蕙凌

碩士 / 國立海洋大學 / 食品科學系 / 87 / Mackerel muscle was homogenized with deionized water at a ratio of 35:70 (w/w) for 2 min and boiled for 10 min then cooled. Protease N was added to hydrolyze the homogenate at 50oC for 2h, then heated at 90oC for 10 min to stop the reaction. The supernatant obtained after centrifugation and filtration was the mackerel protein hydrolysate (MPH). The fraction molecular weight of MPH was distributed from 10 Da~178Da, 200Da, 40Da and 15Da. In vitro studies, MPH ...

Yang, Huey- Chyau, 楊惠喬

碩士 / 國立海洋大學 / 食品科學系 / 87 / Quality changes of raw tilapia meat (sashimi) resulted from postmortem storing at 4oC and 25oC as well as its cooked meat resulted from not water dip-cooking where the raw meat had pre-aged to different extents were investigated. Rigrometer, DSC and various biochemical analyses were adopted to study muscle protein and its related physiochemical changes. Meat eating quality indices, including muscle shortening, dripping loss, water holding capacity (WHC), shear (penetra...

Kao, Yang-Yen, 高揚彥

碩士 / 國立臺灣師範大學 / 生命科學研究所 / 100 / Aquaporins (AQPs) are integral membrane proteins that facilitate water transport across cell membrane. In addition, some type of AQPs was also found to facilitate diffusion of CO2, glycerol, NH3, and urea. Recently, in vitro studies with Xenopus oocytes showed that AQP8 is able to facilitate CO2/NH3 diffusion. However, in vivo study was lacking to support AQP’s function in CO2/NH3 transport. In this study, AQP8AA were identified in the gills and larval skin of zeb...

KO HSIN-CHENG, 柯信成

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 102 / Tilapia (Oreochromis niloticus) is a focus aquaculture of farmed fish in freshwater on the world, and named one of the future major source of animal protein . Tilapia is also important aquaculture species in Taiwan, because of it has high temperature, fast growth, disease resistance, omnivorous and euryhaline and other features which have very suitable for farming in tropical and subtropical regions, as well as improved varieties of single-sex farming success....

Chen-Yun Lee, 李承運

碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 100 / Dragonets (Callionymidae) are benthic fishes living mostly in sandy or muddy bottom. There are 182 species belonged to 10 genera worldwide. Previously the phylogenies based on morphology were reviewed by Nakabo (1982, 1983a b) and Fricke (1983, 1986), but there are still some differences on genera and species classification needed to be resolved. The aims of this study were to review dragonets in Taiwan and using both mitochondrial and nuclear genes to test generi...

Hui-Sheng Young, 楊惠珊

碩士 / 國立臺灣大學 / 漁業科學研究所 / 95 / Abstract We use the transgenic zebrafish TG(fli1：EGFP)y1 that express GFP in vessel endothelial cells to study the function of dll4 (delta-like 4) gene in vascular development. Previous research suggested that dll4j16e1 is a dominant mutation causing disconnected arteries in the caudal fins of adult fish. The dll4/dll4 homozygotes, however, died between 7 to 14 days post fertilization without evident deficiencies in vessels. Hence, my project is to identify causes to...

Gong-Her Wu, 吳恭和

碩士 / 國立清華大學 / 分子與細胞生物研究所 / 94 / Partial or complete genomic duplications have occurred several times during the evolution of vertebrates, resulting in several duplicated genes derived from a common ancestral gene. Ionotropic glutamate receptors (iGluRs) mediate excitatory synaptic transmission in the vertebrate central nervous system. The ionotropic glutamate receptors are classified into three major subtypes, the AMPA-, kainite-, and NMDA-preferring receptors. Zebrafish expresses two duplica...

Ming-Jei Lo, 羅明吉

碩士 / 國立東華大學 / 生物技術研究所 / 92 / Mossabicus tilapia (Oreochromis mossabicus), euryhaline and mouthbrooder, is easily reared and short reproductive cycle becoming a high economic fish. Most recent researches focus on the genetic mapping in tilapia and lacking in functional genes assay. The study was conducted to find morphological changes, development of digestive tract, and metamorphosis or development related genes by using anatomic microscope and PCR-select subtractive hybridization (subtraction P...

CAI,YA-NI, 蔡雅妮

碩士 / 國立臺灣師範大學 / 生物學研究所 / 78 /

CHEN,GUAN-RU, 陳冠如

碩士 / 國立海洋大學 / 水產養殖研究所 / 79 /

WANG, XIAN-JUE, 王先覺

碩士 / 國立臺灣大學 / 生物學研究所 / 74 /

蘇麗文

碩士 / 國立海洋大學 / 食品科學系 / 89 / Two experiments were conducted to quantify the optimum dietary ferric citrate (Fe-citrate) and ferrous sulfate (FeSO4·7H2O) requirements of juvenile hybrid tilapia (Oreochromis niloticus × O. aureus). ExperimentⅠ. Purified diets with 8 levels (0, 10, 30, 50, 100, 150, 200 and 400 mg/kg diet) of supplemental Fe-citrate were fed to tilapia (mean body weight 0.63 ± 0.02 g) for 8 weeks. Each diet was fed to triplicate groups of fish. Results indicated that fish ...

Su-Mei Wu, 吳淑美

博士 / 國立臺灣師範大學 / 生物研究所 / 89 / Abstract The purpose of the present thesis was to study the physiological functions of metallothionein (MT) in developing larvae of tilapia (Oreochromis mossambicus). Firstly, MT was purified and characterized, and the quantitative method for MT was also established. The roles of MT in larval devlopment and metal detoxification were studied. The results were surrmanized as follows: (1) Purification and characterization revealed that tilapia MT, ...

WU,SHU-MEI, 吳淑美

碩士 / 國立臺灣大學 / 海洋研究所 / 78 /

Chi-Chang , Lai, 賴繼昌

碩士 / 國立海洋大學 / 環境生物與漁業科學學系 / 91 / In the present study, the selectivity of gill nets and trammel nets for Kyphosus cinerascens and Kyphosus lembus were estimated from samples collected with nets of 7.8, 8.7,10.0, 10.8 ,12.5 cm(gill nets) and 10.4cm(trammel nets) mesh sizes. Sampling were caughted in the coral reef areas along the coast of Aodi in june and july of 2001 and 2002. The 8.7 cm mesh size was the most efficient for two of the target species. IN gill nets , most fishes were...

Hui=Ling Hsu, 徐惠玲

碩士 / 國立海洋大學 / 食品科學系 / 91 / The study was aimed at quantifying the optimal p-aminobenzoic acid (PABA) requirements of juvenile hybrid tilapia (Oreochromis niloticus ´ O. aureus). Purified diets with 8 levels (0, 40, 80, 120, 200, 400, 800, 1200 mg/kg diet) of supplemental PABA were each fed to triplicate groups of tilapia (mean initial body weight: 0.65 ± 0.01 g) for 8 weeks. Fish fed diets supplemented with 400 and 800 mg PABA/kg had higher (p<0.05) weight gain percent and feed efficiency than ...

Tseng Hsin Chieh, 曾心捷

碩士 / 國立海洋大學 / 食品科學系 / 91 / Two experiments were conducted to estimate the optimum dietary calcium (Ca) requirements of juvenile tilapia, reared in freshwater and in seawater.(Exp. 1) and the dietary phosphorus (P) requirements of tilapia reared in freshwater.(Exp. 2) In Experiment I, purified diets with eight levels (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.7 and 1.0%) of Ca were each fed to triplicate groups of tilapia (mean body weight 0.52 ± 0.01 g) for eight weeks in either freshwater or seawater. Resu...

Chen,Shue Ling, 陳淑鈴

碩士 / 國立海洋大學 / 水產養殖學系 / 81 /

Xu, Xin Liang, 徐新亮

碩士 / 國立海洋大學 / 水產養殖研究所 / 82 /

You-Hui Lin, 林慧宥

碩士 / 國立臺灣海洋大學 / 食品科學系 / 95 / Abstract This study was conducted to determine the dietary pantothenic acid and folic acid requirements of juvenile grouper, Epinephelus malabaricus. Experiment I. Pantothenic acid (PA) was added into basal diet at 0, 5, 10, 20, 40, 60, 100 and 200 mg PA/kg diet providing the actual dietary values of 0, 4.60, 9.89, 19.40, 34.31, 50.24, 98.87 and 192.81 mg PA/kg diet, respectively. Each diet was fed to triplicate groups of fish (initial body weight 15.28 ± 0.3 g) in a...

Jen-Hua Hsueh, 薛人華

碩士 / 國立臺灣大學 / 獸醫學研究所 / 97 / This study described the first isolation of Staphylococcus lugdunensis from sturgeon, Acipenser schrencki, in Taiwan. The pathogenicity experiments were performed by immersion challenge in juvenile sturgeons. Fish were received 8.1×108CFU/mL, 1.95×109CFU/mL and 1.95×109CFU/mL of bascterial suspension and triple duplicate, and the cumulative mortality was 83%, 66.6％and 33.3％, respectively. It revealed chronic pathogenicity of S. lugdunensis for sturgeons. During the ne...

Wan-Li Lin, 林婉麗

碩士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Abstract The study was aimed at quantifying the optimal dietary vitamin B2 requirements of juvenile grouper (Epinephelus malabaricus). Grade levels (0, 2, 4, 6, 8, 12 and 20 mg/kg diet) of riboflavin were added to the basal diet which upon analyzed to contain 1.09, 3.25, 4.62, 6.63, 8.57, 12.46 and 20.32 mg/kg diet, respectively. Each diet was fed to triplicate groups of grouper (mean initial body weight 7.65±0.08 g) for 8 weeks. Results indicated that fish fed d...

Jing-Yi Gu, 古靜怡

碩士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Abstract A growth trial was conducted to investigate the optimal dietary magnesium (Mg) requirements of juvenile hybrid tilapia (Oreochromis niloticus × O. aureus) reared in freshwater and seawater. Semi-purified diets with eight levels (0, 0.003, 0.008, 0.015, 0.03, 0.05, 0.04, 0.05 and 0.07%) of Mg (MgSO4, Showa Chemical Co., Ltd. Japan) were each fed to triplicate groups of tilapia (mean body weight 0.88 ± 0.01 g) reared in either freshwater or seawater for 8 week...

Fu, Ming-Key, 傅民祺

碩士 / 國立海洋大學 / 海洋生物研究所 / 84 / (略)

Yu, Ming Jun, 余明俊

碩士 / 國立臺灣大學 / 漁業科學研究所 / 83 / The effects of environmental Cd and calcium (Ca) on the mRNA expression of metallothionein (MT), Cd accumulation, calcium content and growth in tilapia larvae were examined. In tap water, high Cd concentration had lethal effect on tilapia larvae. The contents of Cd at 96 h was higher in 50 than 150 ug/l Cd treatments, which suggests that failure in self-protective function may lead to death. Both the contents of Cd and Ca o...

Yang, Chi-Hwa or Chi-Hua, 楊濟華

碩士 / 國立臺灣大學 / 漁業科學研究所 / 85 / The present work is to study the modulation of calcium uptake in tilapia larvae ( Oreochromis mossambicus ). Changes in the total length, body weight, yolk diameter, cadmium content, calcium content and calcium influx of tilapia larvae during cadmium treatment and detoxification were examined.(1) In low-calcium medium larvae treated with 150 ug l-1 cadmium showed over 70% morta...

Lu, Fu-I, 盧福翊

碩士 / 國立臺灣大學 / 漁業科學研究所 / 86 / Calcium ion plays an important role for larvae development. The present study, using different calcium concentrations as environmental fators, investigated the calcium balance mechanism in developing tilapia larvae. The abilities to modulate calcium influx in larvae varied with different acculimation periods and developmental stages. In the artificial freshwater with 0.2 mM [Ca2+], tilapi...

Lin, Li-Yih, 林豊益

碩士 / 國立臺灣大學 / 漁業科學研究所 / 86 / The present study is to study the possible role of cortisol in water balance in tilapia (Oreochromis mossambicus)larvae. The seawater(SW)- adapted larvae were immersed in 10 ppm cortisol for 24 h, then wet weight, water cotent, and drinking rate of larva were determined. Cortisol-immersed group showed lower values in wate content, wet weight and drinking rate than those of control group. Howev...

Hsueh-chen Tsai, 蔡雪貞

碩士 / 國立屏東科技大學 / 食品科學系所 / 96 / Taiwan produces 60,000 tons of pacific saury (Cololabis saira) yearly, yet the utilization has been highly restricted due to tiny bones and easy lipid oxidation. This study aimed at investigating the prospect of manufacturing restructured steaks. Restructured pacific saury (Cololabis saira) steaks were prepared by pacific saury mince and frozen marlin (Makaira indica) surimi. The starting materials for the restructured steak would consist of white muscle and whole m...

Jung-Wei Tsai, 蔡鎔蔚

碩士 / 國立屏東科技大學 / 食品科學系 / 93 / The contents of the abstract in this thesis: There is boneless meat about 0.5 cm wide at the both sides of the vertebra in a milkfish, usually being used in milkfish porridge or other snacks. However, the back portion of milkfish has numerous fishbones, which is seldom eaten directly. This portion is mainly used in making milkfish ball. Although dried milkfish floss is tasty, it costs much to remove the fishbones in home-made production. The other way is to use b...

Yu-Sen Wang, 王有森

碩士 / 國立臺北藝術大學 / 美術學系碩士在職專班 / 97 / Summary Rivers and oceans are like mothers to human civilization, for they have nurtured all living beings and have inspired numerous artists to compose masterpieces for centuries. Grown up in a seaside village, I have been very lucky to stay close to seashore and deeply inspired by the magnificent beauty and harmony of the underwater world. With the rhythms of waves, my ideas and inspiration are plentifully aroused. With my neighborhood experience, my lives ar...

Shih, Mulaine, 施慕蘭

碩士 / 國立清華大學 / 材料科學工程學系 / 104 / This study is to investigate the detection of rhythmic electrical signals of adult Zebrafish and its larvae using both three dimensional (3D) gold microelectrode and 3D functionalized biocompatible direct-growth carbon nanotube (CNT) microelectrodes. This study also demonstrates the applicability of 3D microelectrode as a tool to study living organisms pathology in the future. The CNTs were directly grown on 3D gold microelectrodes at 400 °C. With high surface ...

Jhih-Jyun Jhuang, 莊智鈞

碩士 / 國立勤益科技大學 / 電子工程系 / 104 / Fishhook wiring in the production line still relies on labor so far in industry. However, it becomes a big challenge for the factory with increasing labor cost. Therefore, this paper aims to develop the system software for automatic fishhook wiring process along with the mechanism design. The proposed software covers the following major mechanism: 1. Fishhook feeding system (vibration plate). 2. Fishhook wiring system. 3. Finished product collection. 4. Human-machin...

Yi-Jhan Lyu, 呂易展

碩士 / 國立勤益科技大學 / 電子工程系 / 104 / Currently, the fishhook wiring process depends on the human operation only in this industry. In order to improve the production efficiency, the proposed automatic fishhook mechanical wiring system used SolidWorks to design and simulate its 3D performance action. Therefore, it can provide more precision in mechanism before the system is realized. It can also avoid possible interfere between mechanical parts. Beside, PLC is used as the crucial controller, and the touc...

Jhe-Yi Yang, 楊哲一

碩士 / 國立臺灣大學 / 生物環境系統工程學研究所 / 104 / Aquaponics have brought about a new concept for food production since 1970. Aquaponics combined aquaculture and hydroponics, utilizing purified wastewater by nitrification from aquaculture, and use the water for plants. This simplified process can reduce water wasting, and creates a new way of sustainable development for agriculture. However, the nutrients balance between the ecretion from aquatic and the abosorb from the absorption of plants which is the key t...

HSIEH,HUI-SHU, 謝慧淑

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 105 / The objective of present work is to study the process conditions for preparing peptidechelated calciumcomplexes (Peptide-Ca)from collagen peptide of perch scale. Collagen peptides were first obtained from perch scale treated with alcalase plus flavourzyme at 50 oC, pH 7.5 for 2 hrs.The optimal conditions for preparing Peptide-Cawere listed as follows: reaction temperature 50 oC at pH 7.5 for 2 hrs.; ratio of peptide to calcium chloride, 2: 1 and calcium chela...

Chang-Yuan Mo, 莫長原

碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 98 / The purpose of this study is to establish the process conditions for preparing hydrolysate from milkfish in order to develop a marine seasoning. The proteinases of alcalase, bromelain, flavourzyme, papain, pepsin, protamex, protease-N and trypsin were used and compared to their reactivity for preparing hydrolysate from milkfish. Alcalase, protamex and flavourzyme showed higher activity for the hydrolysis of milkfish. Thus, alcalase, protamex and flavourzyme wer...

Chih- Wei Hsu, 許值瑋

碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 99 / There were two aims in this study: (1) to confirm which type of thyroid hormone of tilapia was gifted from maternal origin; (2) when did thyroid hormone start to synthesize in tilapia larvae. We identified that 100 % of the yolk disappeared at 9 dph (day post hatching). We also observed whole body of T3 content was gradually lower before 11dph, but T4 contents can’t be detected at the same stage. Besides, TU can not inhibit T3 synthesis in 0- 13 dph, but inhibited...

Lin Tsung, 宗麟

碩士 / 國立臺灣師範大學 / 生命科學研究所 / 99 / Ammonia excretion in seawater (SW) teleost was rarely studied. In this study, an ion-selective microelectrode technique (SIET) was applied to analyze the ionic (H+ or NH4+) activity at the skin cells of SW-acclimated medaka (Oryzias latipes) larvae. Results showed that H+ and NH4+ were mainly excreted by mitochondrion-rich cells (MRCs). The excretion rate of NH4+ was significantly higher in SW than in FW. Elevated ammonia excretion in SW-larvae alkalized the skin s...

Tsao, Ching-Yu, 曹欽玉

碩士 / 國立海洋大學 / 水產製造學系 / 69 / The studies were carried out for the purpose of tracing the denaturation in muscle proteins, deterioration in freshness and changes in freezing tolerance of frozen mackerel and amber-fish muscle during storage for a year. Fresh mackerel (scomber tapeinocephalus) and amberfish (Decapterus maruadsi) were first washed with tap-water. The whole fish were frozen to body temperature below -18℃ with an air-blast freezer (air tem-perature: -35 ℃, wind velocity: 3.0m/sec.). Su...

Huang Pau-Yu, 黃保諭

碩士 / 國立中興大學 / 農業經濟學系 / 88 / ABSTRACT The influence of quantities of aquatic products by breeding to their prices has a phenomenon of time lag that is reflected by its biological characteristics. The appearance of things comes into existence as the cobweb theory of economics. Consequently, the price of aquatic products by breeding has been seriously impacted, the income of fishing population affected, and development of fishery industry blocked. Because of the conditions as...

林四美

碩士 / 國立海洋大學 / 食品科學系碩士在職專班 / 90 / Abstract The study was conducted to determine the optimum dietary manganese requirement of juvenile hybrid tilapia (Oreochromis niloticus × O. aureus). Purified diets with 8 graded levels (0, 3, 6, 10, 15, 20, 30 and 60 mg/kg diet) of supplemented MnSO4˙H2O were fed to tilapia (mean body weight 0.64  0.02 g) for 8 weeks. Each diet was fed to triplicate groups of fish. Results indicated that no differences (P > 0.05) in weight gain (WG), feed effi...

Yeh-Chen Ning, 倪燕貞

碩士 / 國立海洋大學 / 食品科學系碩士在職專班 / 90 / The study was conducted to determine the optimum dietary copper requirement of juvenile hybrid tilapia (Oreochromis niloticus ×O. aureus). Purified diets with 8 graded levels (0, 1, 2, 3, 4, 8, 12 and 20 mg/kg diet) of supplemented CuSO4 were fed to tilapia (mean body weigh 0.79 ± 0.03g) for 8 weeks. Each diet was fed to triplicate groups of fish. Results indicated that tilapia fed diet supplemented with 2 mg Cu/kg diet had significantly (P<0.05) higher weight ga...

Yu-Jiuan Hung, 黃玉娟

碩士 / 國立海洋大學 / 食品科學系碩士在職專班 / 90 / Tilapia was cultured in fresh water and sea, while black chinese roach only in fresh water were select for this study to investigate the changes of meat texture quality and biochemistry of their dorsal muscle during postmortem storage at 4℃ and 20℃. New indices of meat texture were established, including penetration force（PF）, water holding capacity （WHC）and inosine monophosphate（IMP） content were represented for the chew-ability, juiciness and meaty sweetness （u...

Meng Ju, Chou, 周孟儒

碩士 / 國立海洋大學 / 食品科學系 / 90 / Parrotfish (Scaridae) is a dominant fish species living in coral reefs. It is considered a high priced food fish transported and sold live. Due to the market demand and overfishing, parrotfish have been endangered in nature. Thus created stress to the coral reef by overgrowth of algae on coral, otherwise are cleaned up and consumed by parrotfish. Although, the health of coral reef has gone through annual checkup in Taiwan, and the ecology of parrotfish has also been in...

Liang Si-Mei, 梁思梅

碩士 / 國立臺灣大學 / 動物學研究所 / 92 /

Chen, Wen-Yi, 陳文義

碩士 / 國立海洋大學 / 漁業科學研究所 / 68 /